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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
421

The function of TGF-beta1 in ICUAW and the characterization of Sfrp2, a TGF-beta1 target, in skeletal muscle atrophy

Zhu, Xiaoxi 08 January 2015 (has links)
Transforming growth factor beta 1 (TGF-beta1) ist ein multifunktionales Zytokin, welches eine Rolle in der Sepsis und in der Sepsis-induzierten Myopathie spielen könnte. Weiterhin könnten erhöhte TGF-beta1-Level zur Muskelschwäche, die mit der Intensivpflege assoziiert ist (engl. intensiv care unit-acquired weakness, ICUAW), beitragen. Der TGF-beta1- Signalweg wurde in Skelettmuskelbiopsien von ICUAW-Patienten heraufreguliert. Secreted frizzled related protein 2 (SFRP2) wurde in einer Gen-Set-Anreicherungsanalyse als das am höchsten regulierte Gen identifiziert. Im Mausmodell führten Sepsis und Hunger zu einer verringerten Sfrp2-Expression, während dies in der Denervation-induzierten Skelettmuskelatrophie nicht festzustellen war. In differenzierten C2C12-Myotuben führte TGF-beta1 zu einer verringerten Sfrp2-mRNA- und Proteinexpression. Luciferase-Assays deuteten auf eine TGF-beta1-abhängige Herunterregulation von Sfrp2 hin, welche auf Promoterebene durch mögliche negative regulatorische Elemente im Sfrp2-Promoter vermittelt wurde. Weiterhin wurde eine TGF-beta1 induzierte Muskelatrophie durch transkriptionelle Repression der myosin heavy chain Gene beobachtet. Im Gegensatz dazu veränderte TGF-beta1 nicht den proteasomalen Abbau muskulärer Proteine. Die Genexpression von Tripartite motif containing 63 und F-box only protein 32 war hingegen leicht herunterreguliert. TGF-beta1-induzierte Atrophie in differenzierten C2C12-Myotuben wurde teilweise durch rekombinantes Sfrp2 aufgehoben. Weiterhin wurde eine direkte physikalische Interaktion zwischen Sfrp2 und TGF-beta1 gefunden, welche diesen Effekt verursacht haben könnte. Zusammengefasst lässt sich feststellen, dass der TGF-beta1- Signalweg eine wichtige Rolle in der ICUAW durch Inhibition der myosin heavy chain Expression spielt. TGF-beta1-abhängige Herunterregulation von Sfrp2 könnte zu einer Feedback-Antwort, die das Ausmaß der Atrophie durch TGF-beta1 verstärkt, führen. / Transforming growth factor beta 1 (TGF-beta1) is a multifunctional cytokine that may play a role in sepsis and in sepsis-induced myopathy. Our group speculated that increased TGF-beta1 could contribute to intensive care (ICU)-acquired weakness (ICUAW), a catastrophic muscle disease in critically ill patients. We found that TGF-beta1 signaling in skeletal muscle biopsies of ICUAW patients was upregulated. Secreted frizzled related protein 2 (SFRP2) was the most regulated gene identified by gene set enrichment analysis (GSEA). I then studied the regulation and function of SFRP2 in different skeletal muscle atrophy models. In three mouse models, downregulated Sfrp2 expression was observed in sepsis and starvation, but not in denervation-induced skeletal muscle atrophy. In differentiated C2C12 myotubes, TGF-beta1 downregulated Sfrp2 expression on both mRNA and protein levels. Luciferase assays suggested that TGF-beta1-dependent downregulation of Sfrp2 was mediated at the promoter level through possible negative regulatory elements in the Sfrp2 promoter. I also observed that TGF-beta1-induced muscle atrophy was accompanied by transcriptional repression of myosin heavy chain genes. In contrast, TGF-beta1 did not increase proteasomal degradation of muscular proteins since gene expression of Tripartite motif containing 63 (Trim63) and F-box only protein (Fbxo32) was not upregulated; instead, they were slightly downregulated. TGF- beta1-induced differentiated C2C12 myotube atrophy was partially reversed by recombinant Sfrp2. This inhibitory effect could have resulted from direct interaction between Sfrp2 and TGF-beta1, since I found a physical interaction between these two proteins. Taken together, TGF-beta1 signaling pathway could play an important role in ICUAW via inhibition of myosin heavy chain expression. TGF-beta1-dependent downregulation of Sfrp2 may establish a feedback loop augmenting the atrophic effect of TGF-beta1.
422

Die Funktion von Bx42/Skip im TGF-beta/Dpp Signal Transduktionsweg

Hachoumi, Mounia el 02 July 2007 (has links)
Die Notwendigkeit von Bx42 für Drosophila Entwicklung und seine Beteiligung an unterschiedlichen zellulären Prozessen wurde mit Hilfe von RNA Interference (RNAi) demonstriert. Das ubiquitäre Ausschalten oder die Reduktion der Bx42 Expression mittels RNAi führte dabei zu embryonaler Letalität. Weiterhin führte eine gewebespezifische Induktion von Bx42 in Abhängigkeit der verwendeten Treiberlinien bei unterschiedlichen Temperaturen zu mehreren verschiedenen adulten Phänotypen. Diese Phänotypen waren die Grundlage für die Annahme, dass Bx42 eine Rolle in der Regulation mehrerer verschiedener Zellsignalwege spielt. In der Tat interagiert Bx42 mit den Proteinen des Notch-Signalweges Suppressor of hairless [Su(H)] und Notch intracellular domain (N-IC). Zusätzlich werden bei einer Verminderung von Bx42 die Notch Zielgene cut (ct) und enhancer of split m8 [e(Spl)m8] reprimiert (Negeri et al., 2002). In dieser Arbeit wurde die Beteiligung von Bx42 am TGF-ß/Dpp Signalweg untersucht. Es wurde gezeigt, dass Bx42 mit den TGF-ß/Dpp-Signalweg Proteinen Mad und Medea sowohl in vitro als auch in vivo interagiert. Die dabei verwendeten Methoden waren das Hefe-Zwei Hybrid-Sytem und Ni-NTA-Pulldown-Assays. Domänen der Smad Proteine (Mad und Medea), die für die Interaktion mit Bx42 notwendig sind, wurden mit Hilfe von Deletionskonstrukten untersucht. Es konnte gezeigt werden, dass die stark konservierte MH2-Domäne dieser Proteine für die Interaktion notwendig ist. Zudem belegten Versuche die genetische Interaktion zwischen Bx42 und Medea, in denen ein Bx42-RNAi-Phänotyp durch die gleichzeitige Überexpression von Medea gerettet werden konnte. Es ist bekannt, dass das humane Bx42-Homolog Skip sowohl mit den Proteinen Smad2 und 3 des TGF-ß/Activin Signalweg, als auch mit den Onkogenen Sno und Ski interagiert. Skip wirkt hier als Antagonist der Ski/Sno-Wirkung auf den TGF-ß/Activin-Signalweg und fungiert als Koaktivator (Leong et al., 2001). Die Interaktion zwischen Bx42 und der TGF-ß/Activin-Signalweg Komponente dSmad2, sowie mit dem Onkogen dSno konnte in dieser Arbeit auch für Drosophila bewiesen werden. Die Bedeutung dieser Wechselwirkung muss noch in weiteren Arbeiten analysiert werden. Der Einfluss der Bx42-RNAi-Induktion auf die TGF-ß/Dpp Zielgene distal-less (dll), optomotor blind (omb) und spalt (sal) wurde anhand von Reportergen Untersuchungen mit enhancer-trap-Linien und RNA in situ Hybridisierung untersucht. Es konnte gezeigt werden, dass das Ausschalten von Bx42 die Expression dieser Gene in ähnlicher Weise reprimiert, wie eine Elimination des TGF-ß/Dpp-Signals. Diese Ergebnisse unterstützen die Annahme, dass Bx42 in der Lage ist, TGF-ß/Dpp Zielgene durch eine Wechselwirkung mit Mad und Medea zu aktivieren. / The importance of Bx42 in Drosophila development was demonstrated using Bx42-RNA interference. The ubiquitous downregulation of Bx42 generated embryonic lethality, indicating the importance of this protein in early development. The tissue specific induction of Bx42-RNAi resulted in several different phenotypes depending on the driver line and the temperature at which animals were raised. The phenotypes obtained were the key point for the assumption that Bx42 may play a role in the regulation of a number of different cellular signalling pathways. Indeed, within the Notch signalling pathway Bx42 interacts genetically with Suppressor of hairless [Su(H)] and Notch intracellular domain (N-IC). Additionally, the reduction of Bx42 negatively affected the expression of the Notch target Genes cut (ct) and enhancer of split m8 [e(Spl)m8] (Negeri et al., 2002). In this work, the involvement of Bx42 in the Dpp signalling pathway was investigated. It was shown that Bx42 interacts both in vitro and in vivo, as demonstrated by yeast two hybrid protein-protein studies and Ni-NTA pull-down assays, with the TGF-ß/ Dpp components Mad and Medea. Domains of Smads (Mad and Medea) required for Bx42 interaction were examined using deletion constructs of Smads and the importance of the well conserved MH2 domains of Mad and Medea for this interaction was revealed. Moreover, the rescue of the Bx42-RNAi phenotype by the simultaneous overexpression of Medea demonstrated the genetic interaction between Bx42 and Medea. Furthermore, evidences for the interaction of Bx42 with the TGF-ß/Activin pathway component dSmad2 and with the oncogene protein dSno were obtained from interaction assays. The human homologue of Bx42, Skip, also interacts with Smad2/3 or Sno. The meaning of this interaction in Drosophila has yet to be analysed. The influence of Bx42-RNAi induction on the expression of Dpp target genes distal less (dll), optomotor blind (omb) and spalt (sal) was also investigated using enhancer trap lines and RNA in situ hybridisation. In this way it was proven that these genes are suppressed as they are by elimination of Dpp signalling. These results suggest that Bx42 may be able to modulate positively TGF-ß/Dpp signalling through an interaction with the signalling transducer Mad and Medea.
423

Einfluss von Transforming Growth Factor - beta 1 (TGF-β1) und Hypoxie auf die Expression von Sulfattransportern (SAT-1 und NaSi-1) in den humanen renalen Zelllinien TK173 und TK188 / Influence of Transforming Growth Factor beta 1 (TGF-β1) and hypoxia on the expression of sulfate-transporters (SAT-1 and NaSi-1) in the human renal cell lines TK173 and TK188

von Fintel, Hendrik 10 October 2011 (has links)
No description available.
424

Étude des conséquences fonctionnelles de la mutation SGO1 K23E sur la voie de signalisation TGF-β

Gosset, Natacha 06 1900 (has links)
No description available.
425

Caractérisation moléculaire du syndrome CAID : mise en évidence des rôles non canoniques de SGO1 dans la régulation de la signalisation TGF-β et de l'épigénomique.

Piché, Jessica 07 1900 (has links)
Les contractions rythmiques résultent de l’activité stimulatrice du nœud sinusal dans le cœur et des cellules interstitielles de Cajal (CICs) dans les intestins. Nous avons découvert un nouveau syndrome résultant d’une combinaison de la maladie du nœud sinusal (MNS) et de la pseudo-obstruction intestinale chronique (POIC). Ce syndrome, que nous avons nommé Chronic Atrial and Intestinal Dysrhythmia (CAID), résulte d’une mutation récessive du gène SGO1 (K23E). Cependant, les rôles connus de SGO1 n'expliquent pas l'apparition postnatale du syndrome ni la pathologie spécifique, suggérant que des rôles non canoniques de SGO1 conduisent aux manifestations cliniques observées. Cette hypothèse est supportée par la comparaison de CAID avec les autres cohésinopathies qui présentent principalement des phénotypes développementaux sans ou avec des défauts légers du cycle cellulaire. Ce projet visait à une découverte non biaisée des mécanismes non canoniques expliquant le syndrome CAID en utilisant le dogme de la biologie moléculaire (ADN→ARNm→protéine) comme ligne directrice. Pour ce faire, nous avons effectué des criblages multi-omiques sur des fibroblastes de peau de patients CAID et de contrôles sains. Les résultats des criblages ont été validés par électrophysiologie, étude des voies de signalisation pertinentes, immunohistochimie, pyroséquençage des rétrotransposons LINE-1 et quantification des marques d’histones. Nos études multi-omiques ont confirmé des changements dans la régulation du cycle cellulaire, mais aussi dans la conduction cardiaque et la fonction des muscles lisses. Plus spécifiquement, plusieurs canaux potassiques étaient sous-régulés. L’électrophysiologie a confirmé une diminution du courant potassique rectifiant entrant (IK1). L'immunohistochimie des coupes intestinales de patients CAID a confirmé l’augmentation de l’expression de SGO1 et BUB1, un régulateur de la voie de signalisation TGF-β. De plus, la voie canonique de TGF-β est augmentée et est découplée de la voie non canonique. Au niveau épigénétique, une signature unique d’hyperméthylation et de fermeture de la chromatine a été observée. Ce qui est soutenu par l’augmentation de la méthylation de H3K9me3 et de H3K27me3. En conclusion, le syndrome CAID est associé à plusieurs changements ayant possiblement un effet cumulatif plutôt que d’une seule voie de signalisation dérégulée. Nos résultats désignent la perturbation du courant IK1, la dérégulation de la signalisation TGF-β, l’hyperméthylation de l’ADN et la compaction de la chromatine comme éléments conducteurs potentiels des manifestations cliniques observées. La voie TGF-β et les changements épigénétiques peuvent être ciblées par des médicaments existants, constituant ainsi des cibles thérapeutiques prometteuses pour le traitement du syndrome CAID. / Rhythmic contractions are driven by the pacemaker activity of the cardiac sinus node and the intestinal interstitial cells of Cajal (ICC). We have discovered a new syndrome resulting from a combination of sick sinus syndrome (SSS) and chronic intestinal pseudo-obstruction (CIPO). This syndrome, which we have named Chronic Atrial and Intestinal Dysrhythmia (CAID), results from a recessive mutation in the SGO1 gene (K23E). However, the known roles of SGO1 do not explain the postnatal onset of the syndrome nor the specific pathology, suggesting that non-canonical roles of SGO1 lead to the clinical manifestations observed. This hypothesis is supported by the comparison of CAID with other cohesinopathies which mainly exhibit developmental phenotypes without or with mild cell cycle defects. This project aimed towards an unbiased discovery of noncanonical mechanisms explaining CAID using the molecular biology dogma (DNA→mRNA→protein) as a guideline. We performed multi-omic screens on skin fibroblasts from CAID patients and healthy controls. Screening results were validated by electrophysiology, study of relevant signaling pathways, immunohistochemistry, LINE-1 retrotransposon pyrosequencing, and histone marks quantification. Our multiomics analyses confirmed changes in cell cycle regulation, but also in cardiac conduction and smooth muscle function. More specifically, several potassium channels were downregulated. Electrophysiology studies confirmed a decrease in the inward rectifier potassium current (IK1). Immunohistochemistry in CAID patient’s intestinal sections confirmed overexpression of SGO1 and BUB1, a regulator of TGF-β signaling pathway. Additionally, the canonical TGF-β signaling was increased and decoupled from noncanonical signaling. At the epigenetic level, CAID patient fibroblasts have a unique signature of hypermethylation and chromatin closure. This is supported by the increased methylation of H3K9me3 and H3K27me3. In conclusion, CAID syndrome is associated with several changes that, may have a cumulative effect rather than a single deregulated signaling pathway. Our results reveal the disturbance of the IK1 current, the deregulation of TGF-β signaling, DNA hypermethylation and chromatin accessibility changes as potential conductors of intestinal and cardiac manifestations of CAID syndrome. In particular, the TGF-β pathway and epigenetic changes, may be targeted by existing drugs, thus constituting promising therapeutic targets for the treatment of CAID syndrome.
426

Räumlich-zeitliche Dynamik der laserinduzierten Hsp70-Expression in einem humanen Hautexplantatmodell

Konz, Maximilian 06 October 2016 (has links)
Die Narbenbildung des Hautorgans stellt für die gegenwärtige Medizin weiterhin eine schwierige Aufgabe dar. Die frühzeitige Beeinflussung des Wundheilungspro- zesses hin zu einer verminderten oder narbenlosen Heilung scheint von entschei- dender Bedeutung. Ein vielversprechender Ansatz ist die präoperative Laserthe- rapie und dadurch erzeugte Hitzeschockantwort. Auf molekulare Ebene kommt es u.a. zur Expression von Hitzeschockproteine. Die vorliegende in-vitro Studie beschäftigte sich mit der laserinduzierten Hochregulation des Hitzeschockproteins 70 in den epidermalen Schichten. Hierfür wurden drei nicht ablative Lasersysteme mit insgesamt 12 verschiedenen Parametereinstellungen verwendet (1.540-nm Er:Glass- , 755-nm Alexandrit-, 1.064-nm Nd:YAG-Laser). Mithilfe eines humanen Hautexplantatmodells sollte unter gleichbleibenden Bedingungen Zeitpunkt und Konzentration der maximal induzierten Hsp70-Expression sowie epidermale Schä- digungen dargestellt werden. In der verfügbaren Literatur waren hierzu nur begrenzt Daten vorhanden. Alle drei Lasersysteme zeigten signifikante Hsp70-Expressionen. Der Zeitpunkt der maximalen Hsp70-Expression konnte zwischen Tag 1 und 3 festgehalten werden. Dabei zeigten die Lasersysteme unterschiedliche Hsp70- Maxima und unterschiedliche Epidermisschädigungen. Die Ergebnisse ließen schlussfolgern, dass eine potenzielle präoperative Narbenprävention tendeziell ein Tag vor dem chirurgischen Eingriff und mit den stärkeren Parametereinstellungen des 1.064-nm Nd:YAG Lasers durchgeführt werden sollte.
427

The effect of the TGF-β isoforms on progenitor cell recruitment and differentiation into cardiac and skeletal muscle

Schabort, Elske Jeanne 12 1900 (has links)
Thesis (PhD (Physiology (Human and animal))-- University of Stellenbosch, 2007. / Definition: Stem cells are unspecialised cells with the capacity for long-term self-renewal and the ability to differentiate into multiple cell-lineages. The potential for the application of stem cells in clinical settings has had a profound effect on the future of regenerative medicine. However, to be of greater therapeutic use, selection of the most appropriate cell type, as well as optimisation of stem cell incorporation into the damaged tissue is required. In adult skeletal muscle, satellite cells are the primary stem cell population which mediate postnatal muscle growth. Following injury or in diseased conditions, these cells are activated and recruited for new muscle formation. In contrast, the potential of resident adult stem cell incorporation into the myocardium has been challenged and the response of cardiac tissue, especially to ischaemic injury, is scar formation. Following muscle damage, various growth factors and cytokines are released in the afflicted area which influences the recruitment and incorporation of stem cells into the injured tissue. Transforming Growth Factor-β (TGF-β) is a member of the TGF-β-superfamily of cytokines and has at least three isoforms, TGF-β1, -β2, and -β3, which play essential roles in the regulation of cell growth and regeneration following activation and stimulation of receptor-signalling pathways. By improving the understanding of how TGF-β affects these processes, it is possible to gain insight into how the intercellular environment can be manipulated to improve stem cell-mediated repair following muscle injury. Therefore, the main aims of this thesis were to determine the effect of the three TGF-β isoforms on proliferation, differentiation, migration and fusion of muscle progenitor cells (skeletal and cardiac) and relate this to possible improved mechanisms for muscle repair. The effect of short- and long-term treatment with all three TGF-β isoforms were investigated on muscle progenitor cell proliferation and differentiation using the C2C12 skeletal muscle satellite and P19 multipotent embryonal carcinoma cell-lineages as in vitro model systems. Cells were treated with 5 ng/mℓ TGF-β isoforms unless where stated otherwise. In C2C12 cells, proliferating cell nuclear antigen (PCNA) expression and localisation were analysed, and together with total nuclear counts, used to assess the effect of TGF-β on myoblast proliferation (Chapter 5). The myogenic regulatory factors MyoD and myogenin, and structural protein myosin heavy chain (MHC) were used as protein markers to assess early and terminal differentiation, respectively. To establish possible mechanisms by which TGF-β isoforms regulate differentiation, further analysis included determination of MyoD localisation and the rate of MyoD degradation in C2C12 cells. To assess the effect of TGF-β isoforms on P19 cell differentiation, protein expression levels of connexin-43 and MHC were analysed, together with the determination of embryoid body numbers in differentiating P19 cells (Chapter 6). Furthermore, assays were developed to analyse the effect of TGF-β isoforms on both C2C12 and P19 cell migration (Chapter 7), as well as fusion of C2C12 cells (Chapter 8). Whereas all three isoforms of TGF-β significantly increased proliferation of C2C12 cells, differentiation results, however, indicated that especially following long-term incubation, TGF-β isoforms delayed both early and terminal differentiation of C2C12 cells into myotubes. Similarly, myocyte migration and fusion were also negatively regulated following TGF-β treatment. In the P19 cell-lineage, results demonstrated that isoform-specific treatment with TGF-β1 could potentially enhance differentiation. Further research is however required in this area, especially since migration was greatly reduced in these cells. Taken together, results demonstrated variable effects following TGF-β treatment depending on the cell type and the duration of TGF-β application. Circulating and/or treatment concentrations of this growth factor could therefore be manipulated depending on the area of injury to improve regenerative processes. Alternatively, when selecting appropriate stem or progenitor cells for therapeutic application, the effect of the immediate environment and subsequent interaction between the two should be taken into consideration for optimal beneficial results.
428

Le rôle de la signalisation Wnt dans le phénotype anormal des ostéoblastes ostéoarthrosiques

Chan, Thomas 07 1900 (has links)
L’ostéoarthrose (OA) est une pathologie de forte incidence affectant les articulations. Elle est caractérisée principalement par une dégradation du cartilage articulaire, un déséquilibre au niveau du remodelage osseux et une sclérose de l’os sous-chondral. L’étiologie de cette pathologie reste encore méconnue, cependant il semble de plus en plus que tous les tissus composant l’articulation soient affectés dans cette pathologie. L’importance du rôle de l’os dans le développement de l’OA est incontestable et représente donc une cible thérapeuthique intéressante. Des études effectuées par tomodensitométrie ont démontré une structure et une organisation anormales du tissu osseux des patients OA. Parallèlement, les cultures primaires d’ostéoblastes (Ob) humains OA issus de l’os sous-chondral démontrent un phénotype altéré et une faible minéralisation in vitro. La signalisation Wnt, essentielle dans l’embryogenèse, a montré avoir un rôle clé dans la régulation de l’ostéogenèse en régulant notamment la différenciation terminale des Ob. Le facteur de croissance transformant-β1 (TGF-β1), un facteur agissant notamment sur la prolifération et sur le début de la différenciation des Ob, est surexprimé par les Ob OA et pourrait moduler cette signalisation. Aussi, deux populations de patients OA peuvent être différenciées in vitro par la production de prostaglandines E2 (PGE2) par leurs Ob et les PGE2, dans une étude sur le cancer colorectal, ont montré moduler la signalisation Wnt. Notre hypothèse de travail est que l’activation de la voie de signalisation Wnt/β-caténine est diminuée dans les Ob OA. Cette diminution est responsable de la sous-minéralisation et de l’altération du phénotype des Ob humains OA. Par ailleurs, DKK2, dont l’expression est contrôlée par TGF-β1, est responsable de la diminution de l’activité Wnt/β-caténine et les PGE2 peuvent en partie corriger cette situation. L’objectif général de cette étude est d’une part, de démontrer le rôle de TGF-β1, DKK2 et de PGE2 sur l’altération de la signalisation Wnt/β-caténine et d’autre part, de démontrer le lien entre TGF-β1 et DKK2 et l’effet de ces derniers sur le phénotype des Ob. Dans cette étude on a montré que la signalisation canonique Wnt est altérée dans les Ob OA et que cela était responsable de l’altération du phénotype des Ob OA. On a montré, parmi les acteurs de la signalisation Wnt, que l’expression de l’antagoniste Dickkopf-1 (DKK1) était relativement similaire entre les Ob OA et normaux contrairement à celle de l’antagoniste DKK2 qui était augmentée et à celle de l’agoniste Wnt7B qui était diminuée dans les Ob OA. On a également montré que les PGE2 pouvaient potentialiser l’activité de la signalisation Wnt dans les Ob OA. L’inhibition de DKK2 a permis d’augmenter l’activité de la signalisation Wnt et de corriger le phénotype anormal ainsi que d’augmenter la minéralisation des Ob OA. L’inhibition de TGF-β1, un facteur aussi surexprimé dans les Ob OA, a également permis la correction du phénotype et l’augmentation de la minéralisation dans les Ob OA. L’inhibition de TGF-β1 a aussi menée à l’inhibition de DKK2. Le contraire ne fût pas observé démontrant ainsi la régulation de DKK2 par TGF-β1. En conclusion, la signalisation canonique Wnt est diminuée dans les Ob OA et cela est dû au niveau élevé de DKK2 dans ces Ob. TGF-β1 régule positivement DKK2 et donc la surexpression de TGF-β1 entraîne celle de DKK2 ce qui a pour conséquences d’altérer le phénotype des Ob. Les PGE2 ont aussi montré pouvoir potentialiser l’activité de la signalisation Wnt et auraient donc un rôle positif. Ensemble, ces données suggèrent que ces altérations au niveau des Ob OA pourraient être responsables de la structure osseuse anormale observée chez les patients OA. / Osteoarthritis (OA) is a disease affecting joints and it has a very strong incidence in the population. It is characterized by articular cartilage degradation, an abnormal bone remodelling cycle and subchondral bone sclerosis. The aetiology of this disease is still unknown although OA is now considered as a joint disease involving all the tissues of the joint. The importance of bone in OA pathogenesis is now considered as fundamental and thus bone represent an interesting target for treatments. Tomodensitometric studies have shown an abnormal structure and organisation of the bone tissue of OA patients. In parallel, primary human OA osteoblast (Ob) cultures grown from the tibiofemoral subchondral bone show an abnormal phenotype and a reduced mineralization in vitro. The Wnt signalling pathway, known primarily for its important role in embryogenesis, is of utmost importance in osteogenesis and it has been shown to regulate terminal Ob differentiation. Transforming growth factor-β1 (TGF-β1), known to act on proliferation and on early phases of the differentiation of Ob, is overexpressed by OA Ob and could also modulate the Wnt signalling activity. Furthermore, two populations of OA patients can be discriminated in vitro by the production of prostaglandins E2 (PGE2) of their Ob. In a study on colorectal cancer, PGE2 was shown to modulate the canonical Wnt signalling pathway. Our hypothesis is that canonical Wnt signalling is diminished in OA Ob. This reduction is responsible for the poor mineralization and the abnormal phenotype of human OA Ob. Furthermore, DKK2, overexpressed in OA Ob and whose expression is controlled by TGF-β1, is responsible for the diminution of Wnt signalling activity, and PGE2 can partly correct this situation. The general goal of this study is twofold: 1) to demonstrate the role of TGF-β1, DKK2 and of PGE2 in the Wnt signalling activity; 2) to demonstrate the link between TGF-β1 and DKK2 and their effect on the abnormal OA Ob phenotype. We have shown in this study that the canonical Wnt signalling pathway is altered in OA Ob and that this was responsible for the altered phenotype observed in OA Ob. Also, we have shown that among the mediators of the Wnt signalling pathway, the expression of antagonist Dickkopf-1 (DKK1) was similar between OA and normal Ob. In contrast, the antagonist DKK2 was overexpressed and the expression of the agonist Wnt7B was low in OA Ob. Moreover, PGE2 increased Wnt signalling activity in OA Ob. The inhibition of DKK2 expression also increased Wnt signalling activity and corrected the abnormal phenotype along with increasing the mineralization of OA Ob. The inhibition of TGF-β1 expression, also overexpressed in OA Ob, also resulted in the correction of the phenotype and increased the mineralization of OA Ob. Inhibiting TGF-β1 expression also led to DKK2 inhibition. As the contrary was not observed, this demonstrated that TGF-β1 could regulate DKK2 expression. To conclude, Wnt signalling is reduced in OA Ob and this is due to elevated DKK2 levels in these cells. High levels of TGF-β1 in OA Ob increased DKK2 expression which could be responsible, at least partially, for their altered phenotype. PGE2 was shown to also increase Wnt signalling activity in OA Ob. Taken together these data suggest that such alterations in OA Ob could be responsible for the abnormal bone structure observed in OA patients.
429

The Role of the Stroma and CYR61 in Chemoresistance in Pancreatic Cancer

Hesler, Rachel Anne January 2016 (has links)
<p>Pancreatic ductal adenocarcinoma (PDAC) is a lethal cancer in part due to inherent resistance to chemotherapy, including the first-line drug gemcitabine. Gemcitabine is a nucleoside pyrimidine analog that has long been the backbone of chemotherapy for PDAC, both as a single agent, and more recently, in combination with nab-paclitaxel. Since gemcitabine is hydrophilic, it must be transported through the hydrophobic cell membrane by transmembrane nucleoside transporters. Human equilibrative nucleoside transporter-1 (hENT1) and human concentrative nucleoside transporter-3 (hCNT3) both have important roles in the cellular uptake of the nucleoside analog gemcitabine. While low expression of hENT1 and hCNT3 has been linked to gemcitabine resistance clinically, mechanisms regulating their expression in the PDAC tumor microenvironment are largely unknown. We identified that the matricellular protein Cysteine-Rich Angiogenic Inducer 61 (CYR61) negatively regulates expression of hENT1 and hCNT3. CRISPR/Cas9-mediated knockout of CYR61 significantly increased expression of hENT1 and hCNT3 and cellular uptake of gemcitabine. CRSIPR-mediated knockout of CYR61 sensitized PDAC cells to gemcitabine-induced apoptosis. Conversely, adenovirus-mediated overexpression of CYR61 decreased hENT1 expression and reduced gemcitabine-induced apoptosis. We demonstrate that CYR61 is expressed primarily by stromal pancreatic stellate cells (PSCs) within the PDAC tumor microenvironment, with Transforming Growth Factor- β (TGF-β) inducing the expression of CYR61 in PSCs through canonical TGF-β-ALK5-Smad signaling. Activation of TGF-β signaling or expression of CYR61 in PSCs promotes resistance to gemcitabine in an in vitro co-culture assay with PDAC cells. Our results identify CYR61 as a TGF-β induced stromal-derived factor that regulates gemcitabine sensitivity in PDAC and suggest that targeting CYR61 may improve chemotherapy response in PDAC patients.</p> / Dissertation
430

Studies on Cell Injury Induced by Hypoxia-Reoxygenation and Oxidized Low Density Lipoprotein : With Special Reference to the Protectiove Effect of Mixed Tocopherols, Omega-3 Fatty Acids and Transforming Growth Factor-beta1

Chen, Hongjiang January 2003 (has links)
<p>Hypoxia-reoxygenation (H-R) injury is an important clinical phenomenon in patients with coronary artery disease (CAD). Endothelial injury is a critical step in the initiation and progression of atherosclerosis. Therefore, endothelial and cardiomyocyte protection has been considered an effective step in prevention and treatment of CAD.</p><p>To investigate the cardioprotective effect of tocopherols, omega-3 fatty acid [eicosapentaenoic acid (EPA)] and transforming growth factor-β<sub>1</sub> (TGF-β<sub>1</sub>) during H-R, calcium tolerant myocytes isolated from adult rats were cultured and subjected to hypoxia for 24 hrs followed by reoxygenation of 3 hrs. All strategies, including tocopherol preparations, EPA and TGF-β<sub>1</sub>, showed attenuation of H-R-induced myocyte injury indicated by reduction of lactate dehydrogenase (LDH) release. Both a-tocopherol and a mixed- tocopherols (α-, γ-, and δ-) decreased the effects of H-R on iNOS expression and SOD activity in cultured myocytes. The mixed-tocopherols was more potent than a-tocopherol alone. EPA inhibited H-R-induced lipid peroxidation, MMP-1 expression and p38MAPK phosphorylation. TGF-β<sub>1</sub> blocked the increase in iNOS and PKB phosphorylation as well as the decrease in eNOS expression in cultured myocytes exposed to H-R.</p><p> To further investigate the protective effect of omega-3 fatty acids [docosahexaenoic acid (DHA) and EPA] and TGF-β<sub>1</sub>, the cultured endothelial cells were exposed to oxidant injury mediated by oxidized low-density lipoprotein (ox-LDL). Ox-LDL markedly reduced TGF-β<sub>1</sub> release, increased the expression of TGF-β<sub>1</sub> receptors, upregulated the expression of adhesion molecules, P-selectin and ICAM-1, enhanced the adhesion of monocytes to endothelial cells, and decreased protein kinase B (PKB) activation. Both DHA and EPA blocked these effects of ox-LDL on endothelial cells. Exogenous recombinant TGF-β<sub>1</sub> also ameliorated ox-LDL-induced expression of adhesion molecules and monocytes adhesion, which were blocked by antibodies to the TGF-β<sub>1</sub> type 2, but not to the type 3 receptor.</p><p>These observations provide mechanistic insights into H-R and oxidant injury and tissue protection by three different strategies.</p>

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