by Dai Kang Sheng. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1989. / Bibliography: leaves 127-130.

Diss. (sammanfattning) Stockholm : Karol. inst. / Härtill 7 uppsatser.

Diss. (sammanfattning) Stockholm : Karol. inst. / Härtill 6 uppsatser.

Cyclosporin A immunosuppression following organ transplantation is associated with a reversible nephrotoxicity as manifested by elevations in serum urea and creatinine concentrations and urinary N-acetyl-B-D-glucosaminidase activities. The metabolism of Cyclosporin A is primarily via the hepatic cytochrome P-450 mono-oxygenase system with previous studies having demonstrated that the short term co-administration of phenobarbitone, an inducer of this enzyme system, ameliorates this nephrotoxicity in the non-renal allografted rat. Initial studies demonstrated that the induction of Cyclosporin A nephrotoxicity in the Lewis rat followed by the co-administration of phenobarbitone in the long term abolished nephrotoxicity in the female whilst alleviating it in the male animal. In addition Cyclosporin A hepatotoxicity was also abolished in female rats co-treated with phenobarbitone. A rat renal transplantation model was successfully developed to allow the study of Cyclosporin A nephrotoxicity in the renal allografted animal. Initial studies demonstrated a protective effect of phenobarbitone against Cyclosporin A nephrotoxicity within the first 14 days of treatment in female, but not male allografted animals. These studies also demonstrated a greater susceptibility of the male renal allografted and surgically intact rat to Cyclosporin A induced nephrotoxicity. The effect of ischaemia and sympathetic nervous system denervation were investigated using a series of Lewis syngeneic renal transplanted animals. Histological examination revealed Cyclosporin A induced renal damage to be more marked in non-transplanted than transplanted kidneys. The results are discussed in relation to cold and warm ischaemia and also to the role of the sympathetic nervous and renin-angiotensin-aldosterone systems in the potentiation of Cyclosporin A induced nephrotoxicity. The effect of long term phenobarbitone treatment on Cyclosporin A induced deteriorations in renal and hepatic function was determined using the Lewis syngeneic transplant model. The effect of intraoperative liver ischaemia and diethyl ether anaesthesia on hepatic function and their potentiation of Cyclosporin A hepatotoxicity is also considered. Finally the role of surgical stress and reduction of renal mass by unilateral nephrectomy, on Cyclosporin A nephrotoxicity was studied revealing a protective effect of unilateral nephrectomy against nephrotoxicity in the female animal. Previous studies have demonstrated that reduction in renal mass results in glomerulosclerosis with a progressive impairment of renal function. The protective effect of unilateral nephrectomy reported here is discussed in relation to a Cyclosporin A induced reduction in glomerular filtration rate and subsequent protection against glomerulosclerosis.

Diss. (sammanfattning) Stockholm : Karol. inst., 2001. / Härtill 7 uppsatser.

Diss. (sammanfattning) Stockholm : Karol. inst., 2002. / Härtill 5 uppsatser.

Thesis (Ph. D.)--University of Washington, 1997. / Vita. Includes bibliographical references (leaves [90]-110).

MMed thesis - Faculty of Health Sciences / The field of organ and tissue transplantation has necessitated an improved understanding of their associated pathophysiological pathways. Specific areas of interest involve the changes that follow ischaemia and derangement’s that accompany organ and tissue storage, reperfusion injury and the “no-reflow” phenomenon. Strategies have been devised to manipulate and modify these processes, improving tissue and organ survival and function. These have involved the use of preservation solutions. Although most research involves organ transplantation, these principles have been translated and applied to various tissues, surgical flaps and microvascular replantations. These studies have generally used the skin flap as their model with little knowledge regarding muscle flaps, the most vulnerable to the ischaemic process. This study targets the use of one such preservation system and uses skeletal muscle as its tissue model. The vascular anatomy of the rectus femoris muscle in the New Zealand white rabbit was studied anatomically and radiologically and thus described. The isolated rectus femoris muscle flap was harvested and perfused in-vitro with cooled, oxygenated University of Wisconsin solution (UWS) using a pulsatile renal perfusion pump. UWS was selected as it contains vital additives important in cryopreservation of organs. Monitoring of various physiological parameters was performed. The muscle was examined at 0, 4, 8, 12, 18 and 24 hours of extra-corporeal perfusion using warm and cold, non-perfused controls. The contralateral muscle served as the control. End-points were the percentage of muscle survival, as determined by a new grading system of muscle ischaemia, based on 3 light and 7 electron microscopic criteria. The overall percentage of muscle survival (combined light and electron microscopy scores) resulted in approximately 58% survival at 24 hours for the perfused muscle versus 31% for the cold stored muscle. The stored muscle had the same survival rate at 12 hours as did the perfused muscle at 24 hours. For all time periods beyond 4 to 8 hours, perfused muscle showed statistically improved survival rates compared to the stored muscle. Eight hours appears to be a crucial point beyond which survival in muscle deteriorates to a much greater degree without perfusion. Questions remain as to which method of preservation yields the best survival benefit and, as yet, there is no “ideal” perfusate. The future involves manipulating perfusion solutions and trying to arrest or reverse established warm ischaemia. Success of free tissue transfers and replantations of musclecontaining body parts may be enhanced. These techniques may also allow us to effectively store previously harvested flaps and eventually, to enter the realm of “banked” allograft tissue flaps.

Thesis (doctoral)--Lund University, 1994. / Added t.p. with thesis statement inserted.

Thesis (Ph. D.)--Biomedical Engineering, Georgia Institute of Technology, 2008. / Committee Chair: LaPlaca, Michelle; Committee Member: Bellamkonda, Ravi; Committee Member: Garcia, Andres; Committee Member: Hochman, Shawn; Committee Member: Wang, Yadong. Part of the SMARTech Electronic Thesis and Dissertation Collection.