In this thesis I have attempted to assemble in some coherent form the fruits of two years' research into the renal handling of calcium and phosphorus.

No description available.

The effects of dietary broccoli on the body's ability to detoxify were studied in 18 male subjects between the ages of 22-40 years. The biological parameters used for measuring detoxification were the four major urinary conjugates, namely, mercapturates, sulfoconjugates, glucuronides, and amino acid conjugates. Dietary broccoli increased the urinary excretion of mercapturates and sulfoconjugates, but did not influence the excretion of glucuronides and amino acid conjugates. A significant linear trend was observed over the six-day broccoli diet treatment for both urinary mercapturate (P<0.005) and sulfoconjugate (P<0.0001) excretion. The linear trend for the mercapturate excretion was in a dose-dependent manner, resulting in a 1.3 and 2.1 fold increase by the third and sixth days, respectively, of the broccoli diet, compared to the control. For sulfoconjugates, an unexpected decrease was observed on the first day of the broccoli diet. However, within the six-day broccoli dietary treatment, a continuous increase in conjugate excretion was observed, resulting in a 2.5 fold increase by the sixth day compared to the first day. The excretion of sulfoconjugates was not necessarily dose-dependent, and increased excretion at the highest level of broccoli (500 g) could be due to a time effect. Overall, sulfoconjugate excretion was the highest (3.98-8.91 mmole/24 h) followed by the amino acid conjugates (3.06-5.99 mmole/24 h) and glucuronides (2.85-3.54 mmole/24 h). Mercapturate excretion was the lowest (0.16-0.34 mmole/24 h). In spite of its low excretion level, the level of urinary mercapturates appeared to be the most responsive urinary conjugate to the different levels of broccoli diet. / Ph. D.

In order to establish a profile of detoxification via glutathione conjugation, the level of urinary mercapturic acid excreted by a free-living male population and the effect of external environmental and genetic factors, such as consumption of vegetables, fruits, and meat, charbroiled food intake, tobacco, alcohol, caffeinated coffee, and marijuana use, exposure to chemicals and familial cancer incidence, were investigated. A subgroup of 30 subjects was randomly selected from 117 subjects who complied with the collection protocol. Three consecutive 24 hr urine samples of this subgroup were analyzed. The modified method of Seutter-Berlage et al (Chemical Porphyria in Man. Elsevier/North-Holland Biomedical Press, N.Y. 1979:233-236) was used for the quantitation of urinary mercapturic acid. The mean excretion of mercapturic acid was 0.27 mmole mercapturate (-SH)/24 hr and 18.1 umole -SH/mmole creatinine. An analysis of variance showed a large degree of inter- and intraindividual variability. The interindividual coefficients of variation in mmole -SH/24 hr and umole -SH/mmole creatinine were 37.7% and 31.2%, respectively. The intraindividual coefficients of variation in mmole -SH/24 hr and umole - SH/mmole creatinine were 32.4% and 30.1%, respectively. A higher (p≤0.05) excretion of mercapturic acid was observed among subjects with a high frequency of exposure to chemicals. The lack of significance of the other dietary, non-dietary, and genetic factors on the observed mercapturic acid excretion may be due to the large inter- and intravariability, the use of food consumption frequency in food intake analysis, and unequal sample sizes of subgroups. / Master of Science

The purposes of this research were to: (1) describe selected fluid and electrolyte changes that occur during the first four hours after open heart surgery; (2) determine whether significant hourly changes occurred in: blood pH, fluid intake, exogenous potassum chloride replacement, fluid output, or serum and urinary sodium and potassium levels; and finally, (3) delineate which of the above variables were most strongly related to changes in serum and urinary, sodium and potassium levels. Fifty-three patients who had undergone cardiac surgery involving cardiopulmonary bypass were studied. Urine and blood samples were collected every hour for the first four hours postoperatively and were analyzed for sodium and potassium content using flame photometry. Blood pH and exogenous potassium chloride replacement were recorded from the anesthesiologist's and nurse's records. Fluid intake and fluid output were measured directly by the investigator. Analyses included both descriptive and correlational statistics. In addition, a repeated measures procedure (MANOVA) was performed to discern performance trends over time. The data showed that hypokalemia (defined as a serum potassium level less than 4.0 mEq/L) was present in approximately 52 percent of the subjects for the first two postoperative hours and that by the fourth hour only 15 percent of the subjects were hypokalemic. In addition, subjects were noted to retain 2.47 liters of fluid over the four hour period. Significant differences in fluid output were noted across time. Serum sodium levels did not change significantly across time even though serum potassium levels did. The data indicated that the best predictors of hypokalemia were fluid intake and fluid output. Both of these variables had a significance level of p = .000. Regression analysis showed that fluid intake and exogenous potassium chloride replacement explained 11.9 percent of the variance in serum potassium at a significance level of p = .008. Furthermore, fluid output explained 7.3 percent of the variance in urinary potassium (p = .030).

A dissertation submitted in partial compliance with the requirements for the Master's Diploma in Technology: Homoeopathy, TechniKon Natal, Durban, 1994. / This study contains a review of the major recognizable Industrial problem of chronic lead toxicity. Lead poisoning has been recognized for thousands of years, dating beclt to the Roman Empire. Today, Industries In South Africa and worldwide struggle to maintain low blood lead levels In their employees and companies have to abide by regulations to ensure they ere doing all that Is In their power. Cooltson Chemicals (Pty) Limited, In Durban, which has a factory which produces lead based chemicals, was approached with regard to treating the workers exposed to the lead dust and fumes with Homoeopathlc Slmlllimum. This Individualistic Homoeopathlc treatment depends on the person's symptoms and signs on a physical, mental and emotional level, and by treating the person as a whole, the excretion of toxic lead occurs. Thus lower lead levels result. According to Herneeepetntc methodology, It Is the sum of all the symptoms and signs In each Individual ease of a disease that Is the main pointer In the choice of the remedy. This Is where despite many complaining of a slmillar condition, or suffering from a common complaint, different Homoeopathlc remedies may be prescribed. Lower blood lead levels result In fewer retrenchments as once a factory employee continually presents with excessively high blood lead levels, he risks losing his Job. A sample group of twenty volunteers was obtained from this factory and each werker tooit his prescribed medication and had blood and urine tests talten at regular Intervals over five months. The precise methodology and the consequent results are explained In this paper. The results were analysed using the computer program, SGPLUS,to obtain summary statistics. The blood lead results were subjected to The Wilcoxon Signed RankTest to determine the 11kreasesand decreases In the levels over the monitoring period. Due to sample size and the presence to only one group In the research, the remaining results were from visual observation. Based on the problems encountered during this study, recommendations are made If further research Is to be done along similar guidelines. A final conclusion closes the study. The urine porphyrin tests are not a true Indicator of lead metabolism or excretion and these results are therefore not valid. A slight change did occur In the blood lead levels as Is discussed. A great deal of progress remains to be made with the aim of lowering blood lead levels In lead-exposed factory workers. Homoeopathy has the potential to alleviate this serious problem of chronic lead toxicity. Further research could provide Industry with the answer. Presently the emphasis Is on prevention of excessively high blood lead levels with only removal from exposure as the last possible means of alleviating the problem. If a method to solving lead toxicity In the Industrial sphere can be found, It has numerous possibilities In nonIndustry. Thus an opening exists for Homoeopathy. / M

Typescript (photocopy). / Digitized by Kansas Correctional Industries

Dated 1983. Bibliography: leaves 174-181.

Appendix 7, (3 leaves) in pocket. Includes bibliography.

A reverse-phase high pressure liquid chromatographic method for the quantitative analysis of unchanged ascorbic acid in human urine is described. Selection of an appropriate mobile phase and discussion of some of the analysis problems are presented. Twenty-four hour ascorbate excretion profiles from two subjects were determined. Standard redox titration procedures were used to corroborate the chromatographic method. When compared to classical titration or colorimetric redox procedures, the new assay features straightforward sample preparation and improved sensitivity.Ball State UniversityMuncie, IN 47306