601 |
Epstein-Barr virus characteristics and its correlation with the expression of cytotoxic proteins and cytokines in non-nasal peripheralT-cell lymphomasHo, Wen-ying. January 1999 (has links)
published_or_final_version / Pathology / Doctoral / Doctor of Philosophy
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602 |
Mosaic diseases of Chinese yard-long bean (Vigna sesquipedalis (L.) Fruwirth) in Hong Kong梁家慧, Liang, Kar-wai, Phoebe. January 1968 (has links)
published_or_final_version / Botany / Master / Master of Science
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603 |
Genetic polymorphisms of type I interferon receptor 1 affect the susceptibility to chronic HBV infection: association analysis and mechanistic investigation周婕, Zhou, Jie January 2007 (has links)
published_or_final_version / abstract / Microbiology / Doctoral / Doctor of Philosophy
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604 |
Identification of small molecule inhibitors of influenza A virus by chemical geneticsLau, Lai-shan., 劉麗珊. January 2007 (has links)
published_or_final_version / abstract / Microbiology / Master / Master of Philosophy
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605 |
Set up and validation of an automated PCR diagnostic and surveillance platform for influenza胡婉晶, Wu, Yuen-ching. January 2009 (has links)
published_or_final_version / Microbiology / Master / Master of Medical Sciences
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606 |
Evaluation of anti-human respiratory syncytial virus effects of short interfering RNAs and β-defensin-4Zhang, Ke, 张科 January 2014 (has links)
The human respiratory syncytial virus (hRSV) infection is a global public health burden in children aged under 2 years and immunocompromised or elderly adults. The choice for prophylaxis and therapy of hRSV infection was constrained to Palivizumab and Ribavirin. Therefore, this study aimed to develop effective anti-hRSV infection agents, such as short interfering RNA (siRNA) and β-defensin-4 (β-D-4).
Since there still is no compatible animal model to evaluate antiviral effect of anti-hRSV agents, we first attempted to establish suitable animal model. A clinical isolate of hRSV (KI-RSV-W) was adapted in BALB/c mice by serial passages. Old male mice (age > 8 months) were used for establishment of hRSV infection model, because the more efficient viral infection in lungs of the old male mice than that old female mice and young mice (age < 3 weeks). After the virus was propagated in old male mice for 20 passages, a virus variant KI-RSV-P70-4 exhibited more efficient infection/replication in the mice. Its viral load was about 100-fold higher than that of wild type strain KI-RSV-W. The infection of KI-RSV-P70-4 also caused more severe histopathological changes in lung tissues. Although KI-RSV-P70-4 could not result in death of the infected mice, both viral load and pathological change in lungs may be good indicators for evaluating antiviral effect. The mouse model and adapted hRSV strain solidly laid the foundation for evaluation of anti-hRSV agents.
We then designed and evaluated anti-hRSV effect of siRNAs. A total of 25 siRNAs targeting 4 viral genes (M2-1, NS2, N and F) were designed and their anti-hRSV effect was assessed in vitro. The results showed that 6 siRNAs respectively targeting M2-1, N and F genes exhibited higher anti-hRSV effect than that of the positive control, whereas those targeting NS2 gene did not show significant antiviral effect. The 50% inhibitory concentrations (IC50) of three most potent siRNAs (M2-1-361, N889 and F-1143) were 0.51, 2.14 and 0.64 nM, respectively.
Antiviral activity of β-D-4 against hRSV infection was evaluated in vitro and in vivo. In vitro experiments showed supreme antiviral effect with IC50 around 3.4 μg/ml when the virus was pretreated with β-D-4, but no significant inhibitory effect was observed when the cells were pretreated with β-D-4 or β-D-4 was maintained in the culture medium after viral infection. These results indicated that the inhibitory effect of β-D-4 was associated with direct interaction with the virus itself and blocked virus entry of the cells. Furthermore, a single dose (13.6 μg) of β-D-4 intratracheal (i.t.) administration in old male mice after the viral infection resulted in about 0.7 log reduce of viral load in lung tissues, while inoculation of premixed β-D-4 and the virus caused about 3 logs decrease of viral load in lungs. These results have demonstrated that β-D-4 may be an effective anti-hRSV agent.
Taken together, old male BALB/c mice might be used to establish hRSV infection model. Three siRNAs and the β-D-4 were validated as the potent anti-hRSV agents, respectively. / published_or_final_version / Microbiology / Doctoral / Doctor of Philosophy
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607 |
Structure and assembly of filamentous bacteriophagesRowitch, David Henry January 1987 (has links)
No description available.
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608 |
Expression, purification and structural characterization of the DNA-binding domain of BZLF1Brooksbank, Robert Alan January 1994 (has links)
No description available.
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609 |
Transcriptional regulation of the HPV-16 E6 and E7 oncogenes by the HPV-16 E2 proteinWebster, Kenneth January 2000 (has links)
No description available.
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610 |
Double infections with HSV in the mouseYirrell, D. L. January 1987 (has links)
No description available.
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