The goal of this thesis was to produce an animal model that develops atherosclerotic plaque featuring plaque neovascularization leading to intraplaque hemorrhage and is suitable for noninvasive imaging studies. Several strategies were tested for their effectiveness in producing such plaques in the rabbit aorta, including: a high cholesterol diet, vascular endothelial growth factor injections, therapeutic contrast ultrasound, and balloon catheter injury. It was found that a combination of the high cholesterol diet and balloon injury was able to achieve plaque neovascularization in a manner dependent on circulating plasma cholesterol levels. In addition, a contrast-enhanced magnetic resonance imaging technique implemented in the animal model was able to detect plaque neovascularization and monitor its change over time in a single group of animals. In conclusion, an animal model was created where plaque neovascularization occurs in a predictable fashion and can be studied with non-invasive magnetic resonance imaging.

This thesis presents a high-resolution volumetric technique to measure the longitudinal relaxation time T1 in the vessel wall using MRI. The method of Variable Flip Angles (VFA) was applied using a new strategy for flip angle selection that allows measurement of T1 with high accuracy (< 10% mean error) and precision (T1-to-noise ratio > 10) over the wide range of anticipated values (300-3000ms) in the vessel wall. This strategy was validated in simulation, phantom and volunteer spinal cord experiments. Initial validation of vessel wall T1 measurements was performed in ex-vivo thoracic aorta samples from cholesterol-fed rabbits. For in-vivo vessel wall T1 mapping, the technique was augmented with spatial saturation bands for flow suppression and applied to the carotid arteries of three volunteers. Preliminary results from volunteers suggest that this approach may be useful in characterizing T1 changes associated with high-risk atherosclerotic disease.

Dans la première partie de cette thèse, nous étudions le renversement de l'aimantation de nanofils d'alliage FePt à forte anisotropie magnétocristalline. Lorsque la largeur du fil devient inférieure à la taille des dendrites, nous avons montré qu'il existe une transition du processus de renversement de l'aimantation, de la croissance de dendrites vers la propagation d'une paroi magnétique unique qui renverse tout le fil. Au-delà, la diminution de la largeur du fil jusqu'à la taille caractéristique du désordre et/ou de la rugosité moyenne conduit au renforcement de la coercivité. Ceci conduit finalement dans les fils ultra-fins à un renversement consistant en un mélange de nucléation de domaines et de propagation de parois magnétiques. Dans la deuxième partie, nous rapportons l'utilisation de la magnétorésistance de Magnon (MMR), qui provient de la contribution des magnons à la résistivité, pour mesurer le renversement d'aimantation, dans des nanostructures avec aimantation perpendiculaire (FePt) ou planaire (NiFe). Nous avons montré que la MMR peut être utilisée pour détecter le retournement de l'aimantation dans les nanofils et nano-aimants, et en particulier pour détecter la position d'une paroi magnétique le long d'un nanofil fabriqués à partir d'une couche unique. Enfin, nous étudions dans une dernière partie la dynamique de dépiégeage de paroi magnétique sous champ et sous courant, dans les deux systèmes FePt et NiFe. Nous observons trois types de dépiégeage de paroi, qui dépendent de la nature des défauts ou de la géométrie de la constriction. L'analyse statistique du temps de piégeage montre que le processus de dépiégeage peut être décrit comme procédant d'un chemin simple, de chemins en série, ou de chemins alternatifs. En outre, l'effet du courant sur tous ces mécanismes de dépiégeage s'est révélé équivalent à l'effet du champ appliqué, ce qui permet de mesurer l'efficacité du transfer de spin dans ces systèmes. Keywords: Magnetization reversal, magnon magnetoresistance, domain wall, spins transfer torque.

This thesis describes an investigation of the velocity field in a fluid around a solid sphere undergoing transient motion parallel to, and midway between, two plane walls. Particle Image Velocimetry (PIV) was used to measure the velocity at many discrete locations in a plane that was perpendicular to the walls and included the centre of the sphere. The transient motion was achieved by releasing the sphere from rest and allowing it to accelerate to terminal velocity. <p>To avoid complex wake structures, the terminal Reynolds number was kept below 200. Using solutions of glycerol and water, two different fluids were tested. The first fluid was 100%wt glycerol, giving a terminal Reynolds number of 0.6 which represents creeping flow. The second solution was 80%wt glycerol yielding a terminal Reynolds number of 72. For each of these fluids, three wall spacings were examined giving wall spacing to sphere diameter ratios of h/d = 1.2, 1.5 and 6.0. Velocity field measurements were obtained at five locations along the transient in each case. Using Y to denote the distance the sphere has fallen from rest, velocity fields were obtained at Y/d = 0.105, 0.262, 0.524, 1.05, and 3.15. <p>It was observed that the proximity of the walls tends to retard the motion of the sphere. A simple empirical correlation was fit to the observed sphere velocities in each case. A wall correction factor was used on the quasi-steady drag term in order to make the predicted unbounded terminal velocity match the observed terminal velocity when the walls had an effect. While it has been previously established that the velocity of a sphere is retarded by the proximity of walls, the current research examined the link between the motion of the sphere and the dynamics of the fluid that surrounds it. By examining the velocity profile between the surface of the sphere at the equator and the wall, it was noticed that the shear stresses acting on the sphere increase throughout the transient, and also increase as the wall spacing decreases. This is due to the walls blocking the diffusion of vorticity away from the sphere as it accelerates leading to higher shear stresses. <p>In an unbounded fluid, the falling sphere will drag fluid along with it, and further from the sphere, fluid will move upward to compensate. It was found that there is a critical wall spacing that will completely prevent this recirculation in the gap between the sphere and the wall. In the 80%wt glycerol case, this critical wall spacing is between h/d = 1.2 and 1.5, and in the 100%wt glycerol case the critical wall spacing is between h/d = 1.5 and 6.0.

Phase-contrast magnetic resonance imaging (PC-MRI) is a powerful tool for measuring blood flow and has a wide range of cardiovascular applications. Simulation of PC-MRI from numerical flow data would be useful for addressing the data quality of PC-MRI measurements and to study and understand different artifacts. It would also make it possible to optimize imaging parameters prior to the PC-MRI measurements and to evaluate different methods for measuring wall shear stress. Based on previous studies a PC-MRI simulation tool was developed. An Eulerian-Lagrangian approach was used to solve the problem. Computational fluid dynamics (CFD) data calculated on a fix structured mesh (Eulerian point of view) were used as input. From the CFD data spin particle trajectories were computed. The magnetization of the spin particle is then evaluated as the particle travels along its trajectory (Lagrangian point of view). The simulated PC-MRI data were evaluated by comparison with PC-MRI measurements on an in vitro phantom. Results indicate that the PC-MRI simulation tool functions well. However, further development is required to include some of the artifacts. Decreasing the computation time will make more accurate and powerful simulations possible. Several suggestions for improvements are presented in this report.

Within the confines of this study, numerical simulations of time dependent variation of downdrag forces on the diaphragm walls are analyzed for a generic soil site, where consolidation is not completed. As part of the first generic scenario, consolidation of a clayey site due to the application of the embankment is assessed. Then two sets of diaphragm walls, with and without bitumen coating, are analyzed. For comparison purposes, conventional analytical calculation methods (i.e., rigid-plastic and elastic-plastic soil models) are also used, the results of which, establish a good basis of comparison with finite-element based simulation results. Additionaly, the same generic cases are also analyzed during the stages of excavation, when diaphragm walls are laterally loaded. As the concluding remark, on the basis of time dependent stress and displacement responses of bitumen coated and uncoated diaphragm walls, it was observed that negative skin friction is a rather complex time-dependent soil-structure and loading interaction problem. This problem needs to be assessed through methods capable of modeling the complex nature of the interaction. Current analytical methods may significantly over-estimate the amount of negative skin friction applied on the system, hence they are judged to be over-conservative. However, if negative skin friction is accompanied by partial unloading as expected in diaphragm walls or piles used for deep excavations, then they may be subject to adverse combinations of axial load and moment, which may produce critical combinations expressed in interaction diagrams. Neglecting the axial force and moment interaction may produce unconservative results.

The art on the Berlin Wall has been looked at often for its social and political meaning. Instead, I intend to look at the artwork and text which appeared on the Berlin Wall as art. In this paper I will discuss the formal aspects of the art on the Berlin Wall as well as its import as an example of public art and as a forum created through visual representation.

The aim of this research is to develop an optimisation tool to support the preliminary design of gas turbine combustors by providing a partial automation of the design process. This tool is to enable better design to be obtained faster, providing a reduction in the development costs and time to market of new engines. The first phase of this work involved the analysis of the combustor design process with the aim of identifying the critical tasks that are suitable for being automated and most importantly identifying the key parameters describing the performance of a combustor. During the second phase of this work an adequate design methodology for this problem was defined. This led to the development of a design optimisation Toolbox based on genetic algorithms, containing the tools required for it's proper integration into the combustor preliminary design environment. For the development of this Toolbox, extensive work was performed on genetic algorithms and derived techniques in order to provide the most efficient and robust optimisation method possible. The optimisation capability of the Toolbox was first validated and metered on analytical problems of known solution, where it demonstrated excellent optimisation performance especially for higher-dimensional problems. In a second step of the testing and validation process the combustor design capability of the Toolbox was demonstrated by applying it to diverse combustor design test cases. There the Toolbox demonstrated its capacity to achieve the required performance targets and to successfully optimise some key combustor parameters such as liner wall cooling flow and NOx emissions. In addition, the Toolbox demonstrated its ability to be applied to different types of engineering problems such as wing profile optimisation.

Lignin is a key component of plant secondary cell walls, providing strength to the plant and allowing water transport. Lignin is a polymer of monolignols that are synthesized in the cell and transported into the cellulose rich cell wall. The primary goal of this thesis is to understand the mechanism(s) of monolignol deposition during xylogenesis. The currently accepted theory is that monolignols are exported by Golgi-mediated vesicle delivery to the secondary cell wall. When this theory was re-examined using cryofixed developing pine, quantitative autoradiography showed that monolignols did not accumulate in Golgi but were rapidly translocated from cytosol to cell wall. This suggests alternative mechanisms, such as membrane transporters, work in monolignol export. ATP binding cassette (ABC) transporters were chosen because they transport other secondary metabolites and some ABC transporter encoding genes are highly expressed in lignifying cells. Four candidate ABC transporters were selected in Arabidopsis (ABCB11, ABCB14, ABCB15 from the ABCB/MDR subfamily and ABCG33 from the ABCG/PDR subfamily) and shown to have overlapping, high vasculature expression patterns. Mutants with T-DNA insertions in single ABC transporter genes had no change in lignification of inflorescence stems. However, a reduced polar auxin transport phenotype was detected in mutants of ABCB11, ABCB14 and ABCB15. An additional approach was the use of inhibitors of ABC transporters. A new assay, which was developed to quantify lignification in primary xylem of Arabidopsis roots, demonstrated that ABC inhibitors did not change lignin deposition. Monolignols are exported and polymerized in the polysaccharide matrix of the cell wall, which includes hemicelluloses that may organize monolignols during polymerization. Since diverse lignified cell types are enriched in either G- or S-lignin, I hypothesized that this pattern could reflect different hemicellulose distributions, which was examined using antibody labeling of xylans or mannans in hybrid poplar xylem. While xylans were generally distributed in all secondary cell walls, mannans were enriched in fibers but not in the ray and vessel walls. In summary, during secondary cell wall deposition, monolignols are exported by unknown transporter(s) rather than Golgi vesicles. In developing poplar wood, the monolignols are deposited into diverse hemicellulose domains in different cell types.

No description available.