The entrainment of microbes from agricultural land into overland flow during rainfall events is recognised as an important source of pathogenic microbes to surface water bodies and yet this transport process is poorly understood. In this study, a method has been developed to separate bacteria into the forms in which they have been postulated to exist in overland flow. Then Escherichia coli was used as a model organism to investigate the transported state of bacteria eroded from cowpats and their subsequent transport in overland flow. Simulated rainfall experiments were used to generate runoff direct from cowpats. Concentrations of E. coli in the runoff direct from cowpats were found to be directly proportional to the concentration in the cowpat, regardless of the age of the cowpat. It was also observed that E. coli were predominantly eroded from cowpats as individual cells. The interactions between E. coli and soil particles in overland flow were then examined in a small laboratory scale model system and showed that E. coli attached to large (>45 [mu]m) soil particles were transported significantly less than unattached cells. However, in the runoff from the model system, E. coli were found to be attached mainly to clay particles that were similar in size to the bacterial cells. Furthermore, the transport of E. coli through the model system appeared to follow the transport of a conservative chemical tracer implying that (a) the cells were being transported as a solute with the bulk of the water flow, and (b) that E. coli attached to small clay particles were as mobile in the overland flow as unattached cells. These observations imply that E. coli predominantly interact with small clay particles that are also being carried along in the overland flow. The transport of E. coli at a larger scale was then investigated using 5-metre long, 1-metre wide buffer strips operated under saturation excess conditions. In buffer strips using intact soils and existing pasture cover, E. coli removal was very poor (26 % removal) at the low flow rate of 2 L min⁻� with no removal observed at the higher flow rates of 6 and 20 L min⁻�. E. coli removal rates were increased to 41 % removal at 2 L min⁻� by cultivating the soils, with the removal rate again decreasing with increasing flow rate. E. coli in the overland flow from the buffer strips did not form into large flocs or attach to large soil particles, but were transported in small neutrally buoyant particles that remain entrained in the overland flow. Under saturation excess runoff conditions, E. coli in overland flow were not effectively removed by buffer strips as the small particles are transported either over the soil surface or, through large pores in the soil. This Thesis has shown that E. coli is transported in overland flow in small particle sizes that are difficult to trap or remove from overland flow thereby explaining the high fluxes of faecal bacteria observed in overland flow from agricultural land.

This thesis investigated the final products from the current effluent treatment system for woolscouring (wool washing) plants, namely, (i) sludge produced from the chemical flocculation of solids in the wastewater from the wash bowls, and (ii) concentrated suint (sheep sweat) produced from evaporation of the liquid phase separated from the sludge. In addition, fibrous wastes from the woolscouring process were studied. The aims of the study were to (i) investigate whether suint could be applied in a sustainable way to arable land as a potassium fertiliser, and (ii) assess the conditions under which the sludge could be composted for use as a soil conditioner to return organic matter to soil. Experiments involving suint were performed at both laboratory and glasshouse scales, while experiments involving the solid woolscour wastes were based both in the laboratory and using a small-scale (4.5 m3 total capacity) in-vessel composting unit established at a New Zealand woolscour. Decomposition was measured using net-nitrogen mineralisation and weight loss methods. Suint, the water-soluble contaminants on the fleece, contained high levels of potassium (20% on a dry weight basis) and also appreciable quantities of sulphur, sodium, and chlorine. Biological treatment before evaporation stabilised the resulting suint and improved the consistency of its composition. Suint did not affect the soil processes examined, in that it partly decomposed in soil, did not inhibit the turnover of model organic compounds, did not affect soil properties such as pH and electrical conductivity, and did not lead to increased leaching of mineral nitrogen. Suint was either neutral or positive towards plant performance when applied to soil at a rate of 100 kg potassium per hectare. Suint was therefore judged to be suitable for application to land and could be targeted to soils known to be deficient in potassium or to areas where crops with a high potassium demand are grown. Sludge, composed of dirt (soil particles, faecal matter, and skin and fibre debris) and wool grease, was highly variable in terms of its rate of decomposition, ranging from 0.8 to 27.8% of the initial total nitrogen mineralised over 30 days at 37℃. Fibrous wastes, such as opener (fibre and contaminants removed from the wool by agitation prior to scouring) and scoured wool cleaner (wool fibre and dust removed from scoured and dried wool) wastes, also showed variability in decomposition rates. Sludge decomposition was improved by as much as threefold when co-incubated with fibrous wastes. Although it was shown that the polyacrylamide and pesticide content of sludge did not inhibit its decomposition, the effect of the grease content was not fully understood. Chemical properties of woolscour sludge, such as the carbon to nitrogen ratio, suggested that sludge was a substrate of good resource quality. From a biological perspective, however, the data suggested that woolscour sludge was limited in available nutrients; sludge nitrogen was derived principally from keratin, which decomposed at a low rate resulting in the slow release of mineral nitrogen and low levels of microbial activity. Thus, sludge appeared a poor substrate for composting. However, the results from composting trials indicated that the sludge could be successfully processed after blending with a bulking agent such as sawdust. The blended material showed a 90% reduction in wool grease over 21 days of composting when the moisture content of the composting mass was kept optimal. Compost temperature exceeded 55℃ when wool fibre was added to the blend. Initial results from a case study involving the commercial composting of the entire sludge production (16 tonnes per day) from a New Zealand woolscour indicated that a saleable compost could be produced from a material that would otherwise go to landfill, and served to illustrate the commercial significance of these research results.

Thesis (M.S.)--Auburn University, 2007. / Abstract. Vita. Includes bibliographic references (ℓ. 80)

Thesis (Ph.D.)--University of Tulsa, 1991. / Bibliography: leaves 239-249.

Diss. (sammanfattning) Uppsala : Sveriges lantbruksuniv. / Härtill 5 uppsatser.

Thesis (Ph.D.)--Murdoch University, 2002. / Thesis submitted to the Divison of Science and Engineering. Bibliography: leaves 185-203.

Thesis (Ph.D.). / Written for the Dept. of Mining, Metals and Materials Engineering. Title from title page of PDF (viewed 2008/05/12). Includes bibliographical references.

Thesis (M.S.)--California Polytechnic State University, 2009. / Mode of access: Internet. Title from PDF title page, viewed on March 30, 2009. Major professor: Yarrow Nelson, Ph.D. "Presented to the Electrical Engineering Department Faculty of California Polytechnic State University, San Luis Obispo." "In partial fulfillment of the requirements for the degree of Master of Science in Civil and Environmental Engineering." "March 2009." Includes bibliographical references (p. 133-140). Will also be available on microfiche.

Thesis (Ph. D.)--University of Washington, 1993. / Vita. Includes bibliographical references (leaves 243-254).

Thesis (M. Sc.)--University of Hong Kong, 1999. / Includes bibliographical references.