No description available.

Thesis (M.S.- Watershed Management)--University of Arizona. / Includes bibliographical references (leaves 88-93).

Thesis (M.S.)--Oregon State University, 2006. / Printout. Includes bibliographical references (leaves 101-109). Also available on the World Wide Web.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1957. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 103-109).

The experimental work reported in this thesis quantified the productivity of lucerne over a two-year period (2000-2001) for a Mediterranean climate at Roseworthy in South Australia (34°32′S, 138°45′E), and determined associated dynamics for water and nitrogen in duplex soil. Shoot growth of dryland lucerne was limited primarily by the pattern and amount of incident rainfall, but high temperature (30-35oC) also constrained summer production. These high summer temperatures induced greater production when irrigation was applied, but under the normally dry summer conditions high temperatures combined with soil water deficit (up to 200mm) caused growth to cease. Thus, shoot dry matter yield under rainfed conditions was 4.9 t ha⁻¹ in 2000 (from 7 harvests) and 1.8 t ha⁻¹ in 2001 (from 5 harvests) whereas summer irrigation increased yield to 14.9 t ha⁻¹ in 2000 (7 harvests) and 7.1 t ha⁻¹ in 2001 (5 harvests). Under rainfed conditions the RUE was 0.55 g DM MJ⁻¹ PARi compared with 1.08 g DM MJ⁻¹ PARi in the irrigated treatment in 2000, reducing to 0.4 g DM MJ⁻¹ for the rainfed and 0.7 g DM MJ⁻¹ under limited irrigation in 2001. Lucerne plant population declined from 69 to 20 (plants m⁻²) in the rainfed treatment and the plants partially compensated for this in 2000 by increasing stem density from 300 to 400 m⁻² in 2000 although this declined back to 300 m⁻² in 2001. In all treatments more than 70% of root biomass was in the top 40 cm soil, this was partially due to the vertical distribution of plant available water but also to subsoil constraints to root development below 0.6m. Nevertheless, lucerne was able to extract water and nitrate to 1800 mm soil depth. Large amounts of irrigation >400mm) over summer (Dec 1999-Mar 2000) increased total soil water content, approaching the drained upper limit; causing a 600% increase in shoot dry matter yield, similarly higher growth rate (71 kg DM d⁻¹) and higher RUE (~1.7 g DM MJ⁻¹ ), confirming that water availability was the main constraint to lucerne growth. Delayed benefits of summer irrigation, especially in the subsurface treatment, were also observed later (July to October) when lucerne was able to scavenge excess irrigation water and nitrate stored in the 600-1800 mm soil profile, which resulted in increased shoot growth. Drainage below the effective rooting depth was negligible, even under irrigation, confirming that lucerne can dry soil profiles and reduce deep drainage. Average annual water use efficiency was 9 kg DM ha⁻¹ mm⁻¹ under rainfed conditions compared to ~15 kg DM ha⁻¹ mm⁻¹ under irrigated conditions. Shoot dry mattter production was closely related to evapotranspiration in all treatments, however, under rainfed conditions losses from evaporation were proportionally higher compared to irrigated treatments. Sub-surface drip irrigation proved superior to surface irrigation using 22% less water compared to surface sprinkler irrigation treatment with comparable yields. Biological N₂fixation was strongly related to shoot production with 18 to 27 kg N fixed per tonne of shoot dry matter across all seasons and treatments. Dependence on N₂fixation appeared to be unrelated to soil mineral N concentration and amounts of nitrate in the profile (to 1m) were generally quite low <35 kg N ha⁻¹). Soil water dynamics under both rainfed and surface irrigated treatments were adequately simulated by the Agricultural Production System Simulator (APSIM) with RMSD < 10% of the observed means and R² > 0.80 for the total soil profile (0-2000 mm). Simulation of growth and development was less satisfactory. For example, the RMSD was ~50% of observed mean for shoot biomass (R² = 0.68) in the rainfed treatment, and 36% (R² = 0.77) in the irrigated treatment. Overall, simulation of shoot DM production was close to observed values during the growing season (Apr-Nov), however the model was unable to capture the observed shoot yield in response to summer irrigation, with simulated shoot DM 40% less than the observed value in 2000 and 35% less in 2001. N dynamics were poorly simulated under these soil and climate conditions. Amounts of soil mineral nitrogen (kg NO⁻₃-N ha⁻¹) were adequately simulated in rainfed conditions but consistently over-predicted under irrigated conditions. This evaluation of APSIM highlights both good and poor model performance and the analysis indicates the need for caution when applying the model in situations where observed data is scarce. Areas requiring improvements to the model are identified. Overall this research has improved understanding of the limitations to potential production of lucerne in a Mediterranean environment on duplex soils and shown that APSIM-Lucerne can be used confidently for many applications, particularly soil-water dynamics. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1352515 / Thesis (Ph.D.) - University of Adelaide, School of Earth and Environmental Sciences, 2009

The experimental work reported in this thesis quantified the productivity of lucerne over a two-year period (2000-2001) for a Mediterranean climate at Roseworthy in South Australia (34°32′S, 138°45′E), and determined associated dynamics for water and nitrogen in duplex soil. Shoot growth of dryland lucerne was limited primarily by the pattern and amount of incident rainfall, but high temperature (30-35oC) also constrained summer production. These high summer temperatures induced greater production when irrigation was applied, but under the normally dry summer conditions high temperatures combined with soil water deficit (up to 200mm) caused growth to cease. Thus, shoot dry matter yield under rainfed conditions was 4.9 t ha⁻¹ in 2000 (from 7 harvests) and 1.8 t ha⁻¹ in 2001 (from 5 harvests) whereas summer irrigation increased yield to 14.9 t ha⁻¹ in 2000 (7 harvests) and 7.1 t ha⁻¹ in 2001 (5 harvests). Under rainfed conditions the RUE was 0.55 g DM MJ⁻¹ PARi compared with 1.08 g DM MJ⁻¹ PARi in the irrigated treatment in 2000, reducing to 0.4 g DM MJ⁻¹ for the rainfed and 0.7 g DM MJ⁻¹ under limited irrigation in 2001. Lucerne plant population declined from 69 to 20 (plants m⁻²) in the rainfed treatment and the plants partially compensated for this in 2000 by increasing stem density from 300 to 400 m⁻² in 2000 although this declined back to 300 m⁻² in 2001. In all treatments more than 70% of root biomass was in the top 40 cm soil, this was partially due to the vertical distribution of plant available water but also to subsoil constraints to root development below 0.6m. Nevertheless, lucerne was able to extract water and nitrate to 1800 mm soil depth. Large amounts of irrigation >400mm) over summer (Dec 1999-Mar 2000) increased total soil water content, approaching the drained upper limit; causing a 600% increase in shoot dry matter yield, similarly higher growth rate (71 kg DM d⁻¹) and higher RUE (~1.7 g DM MJ⁻¹ ), confirming that water availability was the main constraint to lucerne growth. Delayed benefits of summer irrigation, especially in the subsurface treatment, were also observed later (July to October) when lucerne was able to scavenge excess irrigation water and nitrate stored in the 600-1800 mm soil profile, which resulted in increased shoot growth. Drainage below the effective rooting depth was negligible, even under irrigation, confirming that lucerne can dry soil profiles and reduce deep drainage. Average annual water use efficiency was 9 kg DM ha⁻¹ mm⁻¹ under rainfed conditions compared to ~15 kg DM ha⁻¹ mm⁻¹ under irrigated conditions. Shoot dry mattter production was closely related to evapotranspiration in all treatments, however, under rainfed conditions losses from evaporation were proportionally higher compared to irrigated treatments. Sub-surface drip irrigation proved superior to surface irrigation using 22% less water compared to surface sprinkler irrigation treatment with comparable yields. Biological N₂fixation was strongly related to shoot production with 18 to 27 kg N fixed per tonne of shoot dry matter across all seasons and treatments. Dependence on N₂fixation appeared to be unrelated to soil mineral N concentration and amounts of nitrate in the profile (to 1m) were generally quite low <35 kg N ha⁻¹). Soil water dynamics under both rainfed and surface irrigated treatments were adequately simulated by the Agricultural Production System Simulator (APSIM) with RMSD < 10% of the observed means and R² > 0.80 for the total soil profile (0-2000 mm). Simulation of growth and development was less satisfactory. For example, the RMSD was ~50% of observed mean for shoot biomass (R² = 0.68) in the rainfed treatment, and 36% (R² = 0.77) in the irrigated treatment. Overall, simulation of shoot DM production was close to observed values during the growing season (Apr-Nov), however the model was unable to capture the observed shoot yield in response to summer irrigation, with simulated shoot DM 40% less than the observed value in 2000 and 35% less in 2001. N dynamics were poorly simulated under these soil and climate conditions. Amounts of soil mineral nitrogen (kg NO⁻₃-N ha⁻¹) were adequately simulated in rainfed conditions but consistently over-predicted under irrigated conditions. This evaluation of APSIM highlights both good and poor model performance and the analysis indicates the need for caution when applying the model in situations where observed data is scarce. Areas requiring improvements to the model are identified. Overall this research has improved understanding of the limitations to potential production of lucerne in a Mediterranean environment on duplex soils and shown that APSIM-Lucerne can be used confidently for many applications, particularly soil-water dynamics. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1352515 / Thesis (Ph.D.) - University of Adelaide, School of Earth and Environmental Sciences, 2009

Thesis (M.A.)--West Virginia University, 2000. / Title from document title page. Document formatted into pages; contains v, 38 p. : ill. (some col.), maps (some col.). Includes abstract. Includes bibliographical references (p. 26-28).

Thesis (M.S.)--California Polytechnic State University, 2010. / Title from PDF title page; viewed on June 10, 2010. Major professor: Lynn E. Moody, Ph.D. "Presented to the faculty of California Polytechnic State University, San Luis Obispo." "In partial fulfillment of the requirements for the degree [of] Master of Science in Agriculture, with a Specialization in: Soil Science." "April 2010." Includes bibliographical references (p. 48-51).

Growth, productivity and fruit quality of grapevines are closely linked to soil water availability. Withholding of water for any length of time results in slowed growth. If drought continues yield may be lost. Vines can be manipulated to stimulate early defence mechanisms by decreasing soil water availability. By using an irrigation technique, which allows for separate zones with different soil moisture status, it is possible to stimulate response mechanisms of the root system which are normally related to water stress. The difficulty of separating 'wet' and 'dry' zones was initially overcome by using split-root plants with root systems divided between two containers. Such experiments on split-root model plants resulted in the development of an irrigation technique termed partial rootzone drying (PRD). Results from irrigation experiments using PRD have shown that changes in stomatal conductance and shoot growth are some of the major components affected (Dry et al., 1996). The idea of using irrigation as a tool to manipulate stress responses in this way had its origin in the concept that root- derived abscisic acid (ABA) was important in determining stomatal conductance (Loveys, 1984). Later experiments on split-root plants have demonstrated that many effects of water stress can be explained in terms of transport of chemical signals from roots to shoots without changes in plant water status (Gowing et al., 1990). The necessary chemical signals are provided by the dry roots, and the wet roots prevent the development of deleterious water deficits. The general hypothesis tested during this study was that partial drying of the root system gives rise to a change in the supply of root-derived chemical signals which determine changes in grapevine physiology, thereby affecting fruit quality. Experiments were conducted on split-root vines (Vitis vinifera L. cvs. Cabernet Sauvignon and Chardonnay) grown in pots of different sizes, on field-grown vines which had either their root system divided by a plastic membrane (Vitis vinifera L. cv. Cabernet Sauvignon on own roots or grafted on Ramsey rootstocks) or conventional vines with a non-divided root system (Vitis vinifera L. cv. Cabernet Sauvignon, Shiraz and Riesling) with a commercial PRD irrigation design. The irrigation treatments were vines receiving water on both sides (control) and PRD-treated vines, which only received water on one side at any time. The frequency of alternation of 'wet' and 'dry' sides was determined according to soil moisture and other influences such as rainfall and temperature. In most of the experiments the irrigation was alternated from one side to the other every 10 to 15 days. Chemical signals from roots: the role of ABA and cytokinins Studies on chemical signals have concentrated on ABA and cytokinins (CK). An improved stable isotope dilution protocol, which enables analysis of ABA and CK from the same tissue sample, was developed. Analysis of cytokinins focused on zeatin (Z), zeatin riboside (ZR), zeatin glucoside (ZG) and iso pentenyl adenine (iP). Roots are relatively inaccessible, particularly in field situations. To enable easier access to roots of field-grown vines, split-root vines were planted in a trench which was refilled with a sandy soil. This created a homogenous soil substrate and did not restrict root growth while still allowing access to roots under field conditions. Analyses of root samples of field-grown vines have shown that cytokinins and ABA may originate in roots and their concentrations can be substantially altered during an irrigation cycle. Alternating soil water conditions showed that [ABA] in roots on the 'dry' side was significantly higher compared with the 'wet' side. Due to a reduction in CK on the 'dry' side of PRD-treated vines, the ratio between ABA and CK was substantially changed during an irrigation cycle. The ABA levels in root tissue and in petiole xylem sap were negatively related to stomatal conductance. This further suggests that ABA, mostly synthesized on the 'dry' side of the root system, might be responsible for a decline in stomatal conductance. Furthermore, a higher pH of petiole xylem sap was observed in PRD-treated vines which may also contribute to the regulation of stomatal conductance. Studies on stomatal patchiness showed that non-uniform stomatal aperture occurred in field-grown vines under natural environmental conditions and was more abundant under PRD conditions. The degree of stomatal opening, determined by using a water infiltration technique, correlated with measurement of stomatal conductance. Exogenous application of a synthetic cytokinin (benzyl adenine) can override the possible ABA-mediated stomatal closure resulting from PRD treatment, providing further evidence for the in vivo role of these growth regulators in the control of stomatal conductance. The effect of benzyl adenine was transient, however, requiring repeated applications to sustain the reversal. In addition, CKs may also be important in influencing grapevine growth. Following several weeks of repeated spray applications with benzyl adenine, it was found that the development of lateral shoots in PRD-treated vines was enhanced compared to PRD-treated vines sprayed with water only. This supports the idea that the reduction in lateral shoot development seen in PRD-treated vines is due to a reduced production of CKs (Dry et al., 2000a). By measuring shoot growth rate it was found that one common feature of PRD-treated vines, which were not sprayed with CK, was a reduction of lateral shoot growth. It can therefore be speculated that the reduction in lateral growth is related to a reduced delivery of cytokinins from the roots. Zeatin and zeatin riboside concentration in shoot tips and prompt buds/young lateral shoots were reduced by the PRD treatment providing further evidence in support of this hypothesis. Water movement from 'wet' to 'dry' roots Roots, being a primary sensor of soil drying, play an important role in long- and short-term responses to PRD. Using stable isotopes of water and heat-pulse sap flow sensors water movement was traced from wet to dry roots in response to PRD. The redistribution of water from roots grown in a soil of high water potential to roots growing in a soil of low water potential may be of significance with regard to the movement of chemical signals and the control of water balance of roots. Measurements of the relative water content (RWC) have shown a slower decline of RWC of the 'dry' roots of PRD vines relative to roots of vines which received no water, despite similar water content in soil surrounding those roots. The redistribution of water may help to sustain the response to PRD for longer periods possibly releasing chemical signals and to support the activity of fine roots in drying soil. Field vines, irrigated with PRD over several growing seasons, altered their root distribution relative to the control vines. PRD caused a greater concentration of fine roots to grow in deeper soil layers and this may contribute to a better water stress avoidance. The effect on root growth may be augmented by the water movement and by the large difference in ABA to cytokinin ratio, which are also known to alter root growth. PRD makes more efficient use of available water In experiments where both control and PRD-treated vines received the same amount of water many differences between the vines were demonstrated. Under conditions where water supply was adequate for both treatments, the stomatal conductance and growth of the PRD-treated vines was restricted as has been observed in many previous experiments. As total water input was reduced, however, the stomatal conductance of PRD-treated vines became greater than control vines, suggesting that the latter were experiencing a degree of water stress, whereas the PRD-treated vines were not. This may have been due to the greater depth of water penetration in the case of the PRD-treated vines, where water was applied to a smaller soil surface area. This distinction between PRD-treated and control vines, at very low water application rates, was also reflected in pruning weights and crop yields which were actually greater in PRD-treated vines. It was concluded that at low water application rates, the PRD-treated vines were more tolerant of water stress and made more efficient use of available water. Reduction in vigor opens the canopy. The initial aim of the research which led to the development of PRD was to achieve better control of undesirable, excessive shoot and foliage growth which, from a viticultural point of view, has many disadvantages. Grapevine shoot growth rate responds very sensitively to drying soil conditions. The irrigation strategy used in the PRD experiments maintained a reduction of both main shoot and lateral shoot growth. In response to PRD a decrease in shoot growth rate and leaf area was observed. Much of the reduction in canopy biomass was due to a reduced leaf area associated with lateral shoots, thus influencing the canopy structure. This was one major factor improving the light penetration inside the canopy. Control of vegetative vigour results in a better exposure of the bunch zone to light and, as a consequence, in improved grape quality. It is likely that changes in canopy density, as a result of PRD, is causing changes in fruit quality components. Anthocyanin pigments such as derivatives of delphinidin, cyanidin, petunidin and peonidin were more abundant in berries from PRD vines; by comparison the concentration of the major anthocyanin, malvidin, was reduced. When leaves were deliberately removed from more vigorous control vines, which improved bunch exposure, the differences in fruit composition were much reduced. This further supports the idea that a more open canopy, in response to PRD, improves fruit quality by affecting the canopy structure. Fruit quality consequently determines the quality, style and value of the finished wine. Wines from this study have been produced and data on wine quality from commercial wineries are also available. Sensory evaluations have demonstrated that high wine quality from PRD-treated vineyards can be achieved without any yield-depressing effects. This study has provided evidence to support the original hypothesis. The major findings were: a) Chemical signals, altered under PRD and mostly originating from roots, play an important role in the root to shoot communication in grapevines. b) The movement of water from 'wet' to 'dry' soil layers may help to sustain chemical signals as a response of grapevines to PRD and to support the activity of fine roots in drying soil. c) A reduction in vegetative growth, in particular of lateral shoots, was sustained using PRD and affected the canopy structure which in turn, due to a better light penetration into the canopy, improved the fruit quality. d) The reduction in irrigation water applied did not have a detrimental effect on grape yield and thus the efficiency of water use was improved. e) Application of relatively low irrigation rates showed that PRD-treated vines were more tolerant of water stress and made more efficient use of available water. / Thesis (Ph.D.)--Department of Horticulture, Viticulture and Oenology, 2000.

Growth, productivity and fruit quality of grapevines are closely linked to soil water availability. Withholding of water for any length of time results in slowed growth. If drought continues yield may be lost. Vines can be manipulated to stimulate early defence mechanisms by decreasing soil water availability. By using an irrigation technique, which allows for separate zones with different soil moisture status, it is possible to stimulate response mechanisms of the root system which are normally related to water stress. The difficulty of separating 'wet' and 'dry' zones was initially overcome by using split-root plants with root systems divided between two containers. Such experiments on split-root model plants resulted in the development of an irrigation technique termed partial rootzone drying (PRD). Results from irrigation experiments using PRD have shown that changes in stomatal conductance and shoot growth are some of the major components affected (Dry et al., 1996). The idea of using irrigation as a tool to manipulate stress responses in this way had its origin in the concept that root- derived abscisic acid (ABA) was important in determining stomatal conductance (Loveys, 1984). Later experiments on split-root plants have demonstrated that many effects of water stress can be explained in terms of transport of chemical signals from roots to shoots without changes in plant water status (Gowing et al., 1990). The necessary chemical signals are provided by the dry roots, and the wet roots prevent the development of deleterious water deficits. The general hypothesis tested during this study was that partial drying of the root system gives rise to a change in the supply of root-derived chemical signals which determine changes in grapevine physiology, thereby affecting fruit quality. Experiments were conducted on split-root vines (Vitis vinifera L. cvs. Cabernet Sauvignon and Chardonnay) grown in pots of different sizes, on field-grown vines which had either their root system divided by a plastic membrane (Vitis vinifera L. cv. Cabernet Sauvignon on own roots or grafted on Ramsey rootstocks) or conventional vines with a non-divided root system (Vitis vinifera L. cv. Cabernet Sauvignon, Shiraz and Riesling) with a commercial PRD irrigation design. The irrigation treatments were vines receiving water on both sides (control) and PRD-treated vines, which only received water on one side at any time. The frequency of alternation of 'wet' and 'dry' sides was determined according to soil moisture and other influences such as rainfall and temperature. In most of the experiments the irrigation was alternated from one side to the other every 10 to 15 days. Chemical signals from roots: the role of ABA and cytokinins Studies on chemical signals have concentrated on ABA and cytokinins (CK). An improved stable isotope dilution protocol, which enables analysis of ABA and CK from the same tissue sample, was developed. Analysis of cytokinins focused on zeatin (Z), zeatin riboside (ZR), zeatin glucoside (ZG) and iso pentenyl adenine (iP). Roots are relatively inaccessible, particularly in field situations. To enable easier access to roots of field-grown vines, split-root vines were planted in a trench which was refilled with a sandy soil. This created a homogenous soil substrate and did not restrict root growth while still allowing access to roots under field conditions. Analyses of root samples of field-grown vines have shown that cytokinins and ABA may originate in roots and their concentrations can be substantially altered during an irrigation cycle. Alternating soil water conditions showed that [ABA] in roots on the 'dry' side was significantly higher compared with the 'wet' side. Due to a reduction in CK on the 'dry' side of PRD-treated vines, the ratio between ABA and CK was substantially changed during an irrigation cycle. The ABA levels in root tissue and in petiole xylem sap were negatively related to stomatal conductance. This further suggests that ABA, mostly synthesized on the 'dry' side of the root system, might be responsible for a decline in stomatal conductance. Furthermore, a higher pH of petiole xylem sap was observed in PRD-treated vines which may also contribute to the regulation of stomatal conductance. Studies on stomatal patchiness showed that non-uniform stomatal aperture occurred in field-grown vines under natural environmental conditions and was more abundant under PRD conditions. The degree of stomatal opening, determined by using a water infiltration technique, correlated with measurement of stomatal conductance. Exogenous application of a synthetic cytokinin (benzyl adenine) can override the possible ABA-mediated stomatal closure resulting from PRD treatment, providing further evidence for the in vivo role of these growth regulators in the control of stomatal conductance. The effect of benzyl adenine was transient, however, requiring repeated applications to sustain the reversal. In addition, CKs may also be important in influencing grapevine growth. Following several weeks of repeated spray applications with benzyl adenine, it was found that the development of lateral shoots in PRD-treated vines was enhanced compared to PRD-treated vines sprayed with water only. This supports the idea that the reduction in lateral shoot development seen in PRD-treated vines is due to a reduced production of CKs (Dry et al., 2000a). By measuring shoot growth rate it was found that one common feature of PRD-treated vines, which were not sprayed with CK, was a reduction of lateral shoot growth. It can therefore be speculated that the reduction in lateral growth is related to a reduced delivery of cytokinins from the roots. Zeatin and zeatin riboside concentration in shoot tips and prompt buds/young lateral shoots were reduced by the PRD treatment providing further evidence in support of this hypothesis. Water movement from 'wet' to 'dry' roots Roots, being a primary sensor of soil drying, play an important role in long- and short-term responses to PRD. Using stable isotopes of water and heat-pulse sap flow sensors water movement was traced from wet to dry roots in response to PRD. The redistribution of water from roots grown in a soil of high water potential to roots growing in a soil of low water potential may be of significance with regard to the movement of chemical signals and the control of water balance of roots. Measurements of the relative water content (RWC) have shown a slower decline of RWC of the 'dry' roots of PRD vines relative to roots of vines which received no water, despite similar water content in soil surrounding those roots. The redistribution of water may help to sustain the response to PRD for longer periods possibly releasing chemical signals and to support the activity of fine roots in drying soil. Field vines, irrigated with PRD over several growing seasons, altered their root distribution relative to the control vines. PRD caused a greater concentration of fine roots to grow in deeper soil layers and this may contribute to a better water stress avoidance. The effect on root growth may be augmented by the water movement and by the large difference in ABA to cytokinin ratio, which are also known to alter root growth. PRD makes more efficient use of available water In experiments where both control and PRD-treated vines received the same amount of water many differences between the vines were demonstrated. Under conditions where water supply was adequate for both treatments, the stomatal conductance and growth of the PRD-treated vines was restricted as has been observed in many previous experiments. As total water input was reduced, however, the stomatal conductance of PRD-treated vines became greater than control vines, suggesting that the latter were experiencing a degree of water stress, whereas the PRD-treated vines were not. This may have been due to the greater depth of water penetration in the case of the PRD-treated vines, where water was applied to a smaller soil surface area. This distinction between PRD-treated and control vines, at very low water application rates, was also reflected in pruning weights and crop yields which were actually greater in PRD-treated vines. It was concluded that at low water application rates, the PRD-treated vines were more tolerant of water stress and made more efficient use of available water. Reduction in vigor opens the canopy. The initial aim of the research which led to the development of PRD was to achieve better control of undesirable, excessive shoot and foliage growth which, from a viticultural point of view, has many disadvantages. Grapevine shoot growth rate responds very sensitively to drying soil conditions. The irrigation strategy used in the PRD experiments maintained a reduction of both main shoot and lateral shoot growth. In response to PRD a decrease in shoot growth rate and leaf area was observed. Much of the reduction in canopy biomass was due to a reduced leaf area associated with lateral shoots, thus influencing the canopy structure. This was one major factor improving the light penetration inside the canopy. Control of vegetative vigour results in a better exposure of the bunch zone to light and, as a consequence, in improved grape quality. It is likely that changes in canopy density, as a result of PRD, is causing changes in fruit quality components. Anthocyanin pigments such as derivatives of delphinidin, cyanidin, petunidin and peonidin were more abundant in berries from PRD vines; by comparison the concentration of the major anthocyanin, malvidin, was reduced. When leaves were deliberately removed from more vigorous control vines, which improved bunch exposure, the differences in fruit composition were much reduced. This further supports the idea that a more open canopy, in response to PRD, improves fruit quality by affecting the canopy structure. Fruit quality consequently determines the quality, style and value of the finished wine. Wines from this study have been produced and data on wine quality from commercial wineries are also available. Sensory evaluations have demonstrated that high wine quality from PRD-treated vineyards can be achieved without any yield-depressing effects. This study has provided evidence to support the original hypothesis. The major findings were: a) Chemical signals, altered under PRD and mostly originating from roots, play an important role in the root to shoot communication in grapevines. b) The movement of water from 'wet' to 'dry' soil layers may help to sustain chemical signals as a response of grapevines to PRD and to support the activity of fine roots in drying soil. c) A reduction in vegetative growth, in particular of lateral shoots, was sustained using PRD and affected the canopy structure which in turn, due to a better light penetration into the canopy, improved the fruit quality. d) The reduction in irrigation water applied did not have a detrimental effect on grape yield and thus the efficiency of water use was improved. e) Application of relatively low irrigation rates showed that PRD-treated vines were more tolerant of water stress and made more efficient use of available water. / Thesis (Ph.D.)--Department of Horticulture, Viticulture and Oenology, 2000.