Malgré de nombreux travaux menés, les mécanismes d’assemblage des espèces adventices en communautés restent à identifier. Or cette compréhension est cruciale pour concevoir des stratégies de gestion permettant de préserver la biodiversité des plantes adventices tout en maintenant la production agricole. Ce travail de thèse avait pour objectif de comprendre les mécanismes à la base de la structure fonctionnelle des plantes adventices dans les agroécosystèmes. Trois axes de recherche ont été menés. Le premier axe a permis de proposer un cadre d’analyse général des mécanismes d’assemblage à l’origine de la diversité fonctionnelle dans le cadre de l’approche dites modèle nul. Le second axe, basé sur des analyses empiriques, a mis en évidence (1) le rôle prépondérant des processus stochastiques dans l’assemblage des espèces adventices entre types de culture dans les parcelles agricoles et (2) le maintien d’une flore fonctionnellement plus diversifiée due à la coexistence de stratégies de tolérance et d’évitement de l’ombrage en interface, compartiment adjacent au champ. Enfin, dans le troisième axe, nous avons montré expérimentalement que la compétition entre la plante cultivée et la communauté adventice promeut une unique stratégie d’évitement de l’ombrage se traduisant, à l’échelle individuelle, par un mimétisme fonctionnel des traits de la plante cultivée par les plantes adventices les plus performantes. Ainsi, processus stochastiques et déterministes influencent l’assemblage des communautés adventices, et ce à différentes échelles spatiotemporelles. / Despite numerous studies, weed community assembly mechanisms still remain unidentified. However, a better understanding of these mechanisms is crucial to design management strategies promoting weed biodiversity while maintaining agricultural production. The aim of this PhD was to understand the mechanisms underlying the functional structure of weeds in agroecosystems.Three research axes were conducted. The first axis enabled to propose a general analytical framework of community assembly in the context of a statistical approach called null model. The second axis, based on empirical analyses, revealed (1) the predominant role of stochastic processes in weed community assembly between crop types and (2) the coexistence of two, shade-tolerance and shade-avoidance, ecological strategies, thus explaining the higher functional diversity observed in crop edge, a field element adjacent to the field. Finally, on the third axis, based on experimentation, we have shown that the competition between the crop and the weed community promotes a unique shade-avoidance strategy resulting on a functional mimicry of the crop by the most successful weed plants. Thus, both stochastic and deterministic processes influence weed community assembly, besides at different spatial and temporal scales.

The contamination of heavy metals in the environment is a looming concern worldwide. Egeria densa (Planch) (Submerged aquatic plant) from two ponds: Site A with co-ordinates (32º 48’22.04”S; 26°48’58.79” E) and Site B with co-ordinates (32°48’33.25”S; 26°48’33.25”S) in Alice (Eastern Cape) was evaluated for its ability to absorb heavy metals, phytochemical constituents, antimicrobial activity and ultra-structure using standard analytic procedures. Cadmium (Cd), copper (Cu), iron (Fe), lead (Pb), manganese (Mn), and zinc (Zn) were measured in water, sediments and plant. The concentrations of these metal elements were determined with use of Inductively Coupled Plasma- Optical Emission Spectrometry (ICP-OES). In sediments, the heavy metals (mg/kg) decreased in the order of their average concentration as follows: Fe (40.320) > Zn (1.259) > Pb (0.564) > Mn (0.186) > Cu (0.037) in Pond 1 whereas in Pond 2 Fe (61.527) > Cd (0.999) > Mn (0.648) > Pb (0.586) > Zn (0.156) > Cu (0.045). The highest concentration of Fe was detected in both sites and Cu being the least. The concentrations of the metals in the plants sample (from Pond 1) were found in order of Mn > Pb > Cu > Fe whereas cadmium and zinc were not detected, while the concentration in Pond 2 decreases in order of Zn > Mn > Pb > Cd > Fe > Cu. In the water samples, concentrations of heavy metals (mg/L) decreased in the order of their average concentrations as follows: Pb (35.36) > Fe (3.07) > Mn (0.238) > Cu (0.104), both cadmium and zinc were below the limit of detection in Pond 1, whereas in Pond 2 the concentrations decreased as follows: Pb (13.033) >Fe (1.69) > Cu (0.270) > Mn (0.248) > Cd (0.004) and Zinc was not detected. Phytochemical analyses of the plant extracts revealed the presence of phenols, flavonoids, proanthocyanidin, flavonols, saponins, alkaloid and tannins in all the extracts (water, acetone and n-hexane). Both acetone and water extracts, showed high concentration of proanthocyanidin, while tannin was the lowest in acetone extract. Antimicrobial evaluation using, Gram positive (Staphylococcus aureus, Bacillus pumilus, Bacillus cereus, Streptococcus pyogenes, Enterococcus faecalis) and Gram negative (Klebsiella pneumonia, Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris and Serratia marcescens) bacteria showed negative results for all the strain, except Streptococcus pyogenes which was inhibited at MIC of 0.1 mg/ml. Scanning electron microscopy (SEM) of ultra-structure of Egeria densa, showed that certain bacteria attached to the leaf, However more work has to be done on E. densa to verify the mechanism by which it accumulates heavy metals. The study shows that E. densa has a potential of accumulating heavy metals especial Manganese in plant.

Low cover crop biomass production is a major obstacle to the success of conservation agriculture currently promoted as panacea to the inherent problem of soil erosion and loss of soil productivity in the Eastern Cape (EC). Therefore, this study evaluated cover crop management strategies for optimizing biomass production for better soil cover, soil nitrogen (N) and phosphorus (P) fertility, weed control and maize yields. The strategies tested are cover crop bicultures, selection of an adapted lupin cultivar and seeding rate, and the feasibility of rain fed winter cover cropping. The cover crop experiments were carried in rotation with summer maize between the winter of 2009 and summer of 2010/2011. Biculture trial was carried out by seeding oat (Avena sativa) and vetch (Vicia dasycarpa) at three mixture ratios and as sole crops under irrigation. On a separate irrigated trial, two lupin cultivars (Lupinus angustifolius var Mandelup & Qualinock) were seeded to a range of seeding rate, 40 to 220 kg ha-1. To study the feasibility of rain fed winter cover cropping, oat, vetch, rye (Lolium multiflorum), barley (Hordeum vulgare), radish (Raphanus sativa) and triticale (Triticale secale) were relayed into a maize crop in February, March and April of 2010. The irrigated trials were followed with SC701 maize cultivar, whilst the rain fed trial was followed with DKC61-25 maize cultivar. Bicultures gave higher cover crop biomass than sole vetch, increasing with an increase in the oat component of the mixture. Increased N and P uptake was observed with bicultures compared to sole oat, however, the levels were comparable to sole vetch. Sole vetch increased soil inorganic N and P at maize planting, whilst the slow decomposition by sole oat residue resulted in mineral lock up. Bicultured cover crop residues had intermediate decomposition rates and resulted in optimum levels of inorganic N and P for prolonged periods compared to sole crops. Weed suppression by the bicultures was comparable to sole cover crops. Biculturing technology significantly (P<0.05) increased maize grain yield compared to sole oat and the yields were comparable to those from sole vetch. For lupins, 206 kg ha-1 seeding rate gave the optimum biomass yield. Weed dry weights in both cover crop and maize crop decreased with an increase in lupin biomass. Comparable soil total N and inorganic P values at maize planting, were observed from plots planted to 120, 180 and 220 kg ha-1. Maize grain yield increased with an increase in lupin seeding rate. The study on rain fed winter cover cropping had most cover crop species’ biomass decreasing with each delay in planting except for radish, which increased. Vetch produced the highest amounts of biomass from February and March planting whilst radish had the highest biomass in April planting. The two species resulted in the greatest N improvement compared to the other species. Regardless of the grazing, the grass specie residues managed to persist to the next cropping season and the residue remaining were comparable to that of radish and vetch. Late-planted cover crops had the greatest residue remaining than early-planted, as a result, April planted cover crops provided better weed suppression than March and April planted. Vetch provided the highest maize grain yield (4005 kg ha-1) whilst all other species tested had comparable grain yields. The results suggested that bicultures could be grown to give sufficient biomass for both weed suppression and soil fertility improvement. Furthermore, increasing lupin plant densities improve its function as a cover crop with respect to weed suppression, soil fertility improvement and maize yields. The study also showed that for dry land systems, February and March planted vetch and April planted radish can provide the greatest biomass and maize yield improvement.

Achieving high biomass yields of cover crops has been a challenge to the success of Conservation Agriculture (CA) practices in the Eastern Cape (EC). A study was conducted to evaluate strategies for optimizing cover crop biomass production. Trials were carried out to screen summer and winter cover crops, as well as evaluate intercropping patterns and planting dates for biomass, weed suppression and subsequent maize yield under irrigation. Four summer legume cover crop species were evaluated under a Randomised Complete Block Design (RCBD) design. The cover crops were fertilized with 13.34 kg ha-1 of N, 20 kg ha-1 P and 26.66 kg ha-1 K. In the 2008/09 summer season a maize crop was superimposed on the 2007/08 screening trial under no-till. The crop was fertilized with 60 kg ha-1 of N. An intercropping trial was conducted over two seasons as a way of investigating the best way of incorporating cover crops into farmers cropping systems. This was done bearing in mind the limitation of resources such as land. The trial evaluated 3 factors laid as a 2 x 2 x 3 factorial arranged in a split-plot design. The main factor was cover crop planting date (planting at maize planting or 2 weeks after maize planting). The sub plot factor was intercropping pattern (strip intercropping and between row intercropping). A trial was also conducted to evaluate the effect of planting date (End of April and mid May) and four winter legume cover crop species on cover crop biomass, weed suppression and maize grain yield. The experiment was laid out as a Randomised Complete Block Design (RCBD) replicated 3 times. In the subsequent summer season a maize crop was superimposed on the winter trial to test the residual effects of the cover crop species. Another study was conducted to evaluate winter cereal cover crop species for biomass accumulation, weed suppression and subsequent maize grain yield. The cover crops as well as a weedy fallow control plot treatments were laid out as a Randomised Complete Block Design replicated 3 times. In the subsequent summer season a maize crop was superimposed on the site under no-till to evaluate the residual effect of the cover crops on maize. The results showed sunhemp, cowpea and lablab as the best cover crops with high biomass and weed suppression whilst mucuna was the least. Sunhemp consistently yielded higher cover biomass averaging 11200 kg ha-1 over the two seasons whilst mucuna had a consistently lowest average biomass yield of 4050 kg ha-1. These cover crops were above the critical 6 t ha-1 for effective weed suppression. There was a significant (p<0.01) relationship of cover crop dry weight and weed dry weight in both seasons. Subsequent maize grain yield was significantly higher in the sunhemp plots (64.2 %) than the weedy fallow plot. Mucuna, lablab and cowpea had maize grain yield increases of 16.6%, 33% and 43.2% respectively. Intercropping cover crops at maize planting yielded higher cover crop dry weights than a delay in intercropping cover crops. A delay in intercropping resulted in significantly higher average maize grain yield of 4700 kg ha-1 compared to intercropping at maize planting (3800 kg ha-1) and sole maize (4300 kg ha-1) over the two seasons. Strip intercropping also yielded higher (5000 kg ha- 1) average maize grain yield compared to row intercropping (3600 kg ha-1) and sole maize (4300 kg ha-1). There was a significant (p<0.05) relationship between cover crop dry weight in the 2007/08 season and maize grain yield in the 2008/09 season. Early planting grazing vetch gave the highest biomass yield of 8100 kg ha-1 whilst early planted red clover had the lowest biomass of 635 kg ha-1. Low weed dry weights were also obtained from the early planted grazing vetch as opposed to the other treatments. There was a significant (p<0.001) relationship of cover crop dry weight and weed dry weight. In the subsequent 2008/09 summer season early planted grazing vetch had the highest maize yield of 7500 kg ha-1 which was 56.3 % more than the weedy fallow plot had 4800 kg ha-1. The weedy fallow plot also had high weed infestation than the cover crop plots. There were significant (p<0.01) relationships between cover crop dry weight and maize grain yield, winter weed dry weight and maize grain yield and summer weed dry weight and maize grain yield. The results also showed triticale (13900 kg ha-1) as the best winter cover crop for biomass production. Italian ryegrass (6500 kg ha-1) produced the least amount of biomass. In The subsequent maize crop white oats gave highest maize grain yield (6369 kg ha-1) which was 33 % more than the weedy fallow plot (4784 kg ha- 1). There were also significant (p< 0.01) relationships of maize grain yield and winter weed dry weight, maize grain yield and summer growing weeds. The various studies demonstrated that there is opportunity for high biomass production under small scale farmers irrigated conditions using cover crops both in winter and summer. Best bet cover crops were sunhemp, cowpea and lablab for summer and triticale, white oats, barley, Italian ryegrass and grazing vetch for winter. Cover crops can also be incorporated into farmers cropping systems as sole crops or intercrops within the maize based cropping systems. Strip intercropping can be used by farmers as a way of introducing cover crops. Critical to achievement of high biomass is the time of planting cover crops with high biomass when planting is done early. A 2 week delay in strip intercropping cover crop into maize can be used as a way of incorporating cover crops into farmers cropping systems with minimal maize yield reduction.

Aphthona spp. flea beetles were released in the Little Missouri National Grasslands (LMNG) in western North Dakota in 1999 to control leafy spurge (Euphorbia esula L.). The changes in soil seed bank composition and leafy spurge density were evaluated on two ecological sites five (2004) and ten years (2009) after Aphthona spp. release to monitor the effectiveness of the insects on weed control and associated change in plant communities. In 2009, leafy spurge stem density averaged 2 and 9 stems m-2 in the loamy overflow and loamy sites, respectively, compared to 110 and 78 stems m-2, respectively, in 1999 and 7 and 10 stems m-2, respectively, in 2004. Leafy spurge constituted nearly 67% of the loamy overflow seed bank in 1999 compared to 17% in 2004 and 2% in 2009. In the loamy seedbank, the weed represented nearly 70% in 1999 compared to approximately 11% in 2004 and 15% in 2009. As leafy spurge was reduced, native species diversity and seed count increased ten years following Aphthona spp. release. High-seral species represented 17% of the loamy overflow seedbank in 2009, an increase from 5% in 1999. However, Kentucky bluegrass, a non-target weedy species, increased over 250% in the loamy overflow seedbank from 2004 to 2009. The reestablishment of native plant species has often been slow in areas where leafy spurge was controlled using Aphthona spp. A bioassay was completed to evaluate native grass establishment when grown in soil from Aphthona spp. release and non-release sites throughout North Dakota. Native grass production was not affected when grown in soil collected from established Aphthona spp. sites (1.5 g per pot) compared to soil without insects (1.6 g per pot). The cause of reduced native grass production in sites with Aphthono spp. previously observed is unknown but may have been due to a chemical inhibition caused by the insects within the soil that no longer exists. The native warm-season switchgrass (Ponicum virgotum L.) may be an alternative to corn for efficient biofuel production; however, control of cool-season grassy weeds has been a problem in switchgrass production. Various herbicides were evaluated for smooth bromegrass (Bromus inermis Leyss.) and quackgrass [Elymus repens (L.) Gould] control in an established switchgrass stand near Streeter, ND and a weed-infested field in Fargo, ND. Switchgrass yield was higher than the control 14 mo after treatment (MAT) when aminocyclopyrachlor or sulfometuron were applied early in the growing season, but no treatment provided satisfactory long-term grassy weed control. Herbicides were reevaluated at increased rates for smooth bromegrass or quackgrass control in Fargo. Sulfometuron provided 99% smooth bromegrass control when applied at 280 g ha-1 in the fall but injured other grass and forb species as well. Sulfometuron would likely be injurious to switchgrass and could not be used for biofuel production. Aminocyclopyrachlor did not injure other grass species but only reduced smooth bromegrass control by 76% when applied at 280 g ha-1 in the fall. No treatment provided satisfactory long-term quackgrass control.

MENVM / Department of Ecology and Resource Management

No description available.

<p>  </p> <p>Environmental conditions, like light quality and herbivory, can induce changes in plant physiology. Light quality can be manipulated using LEDs (light emitting diodes) to improve crop quality and yield in horticultural production. The manipulation of light quality has also been utilized in the management of agricultural insect pests. Previous research has provided evidence that changes in light quality can have effects on the growth and chemistry of cannabis crops (<em>Cannabis spp.</em>). Varieties of cannabis are grown for the production of the valued cannabinoids CBD and THC. Pest of cannabis include herbivorous arthropods that reduce hemp yield and quality. Arthropod herbivory can induce chemical and physical changes in plants. This thesis further investigates the role of light quality on both cannabis biology and the crop-pest interaction between cannabis and the cannabis aphid (<em>Phorodon cannabis</em>). In the experiments described herein, the effects of light quality and <em>P. cannabis </em>herbivory on cannabis were assessed by measuring impact on biomass accumulation, yield, and the production of CBD and THC. The effects of light quality on the life history traits of <em>P. cannabis </em>were also measured. Cannabis grown under red-blue light quality saw a reduction in vegetative growth but no reduction in yield. Light treatments had no measured effect on the concentrations of CBD and THC in the cannabis buds. Aphid herbivory decreased bud yield but increased vegetative growth. Aphid herbivory reduced the concentration of both CBD and THC in cannabis bud tissue. Under blue-rich light quality, <em>P. cannabis </em>experienced higher reproductive rates when compared to red-blue and full-spectrum lighting. </p>

No description available.

Methyl bromide (MBr) was widely used as a soil fumigant to manage soilborne pests in plasticulture vegetable production; however, it has been banned by the United Nations Environment Programme. Alternatives to MBr must be implemented to sustain fresh market tomato productivity. Possible MBr alternatives include new fumigant compounds, improved plastic mulch, and grafting. Methyl iodide (MeI) and dimethyl disulfide (DMDS) were tested as fumigant alternatives to MBr for the control of yellow nutsedge and soilborne pathogens of tomato. Virtually impermeable film (VIF) and totally impermeable film (TIF) were tested for fumigant retention and yellow nutsedge control in tomato. Grafting onto resistant rootstocks was tested for bacterial wilt and nematode management in tomato. In the absence of a soil fumigant, TIF suppressed yellow nutsedge better than VIF. TIF increased fumigant retention compared to VIF at similar application rates. Reduced fumigant application rates could be used in combination with TIF while maintaining fumigant concentrations, weed control, and crop yields comparable to greater use rates with VIF. Shank applied DMDS rates could be lowered to 281 L/ha under TIF from 468 L/ha under VIF; shank applied MeI application rates could be reduced to 56 L/ha under TIF compared to 93 L/ha under VIF and drip applied DMDS could be reduced from 561 L/ha under VIF film to 374 L/ha under TIF. Grafting susceptible commercial tomato cultivars onto resistant tomato hybrid rootstocks increased yields and plant survival in bacterial wilt infested fields. "Cheong Gang", "BHN 998", and "BHN 1054" were the best performing rootstocks for bacterial wilt resistance and tomato fruit yield in severely infested fields. Grafting increased tomato yield and decreased root galling from root-knot nematodes in an infested field. Hybrid rootstock "RST 106" resulted in the lowest root-knot nematode galling. In conclusion, TIF with reduced rates of DMDS or MeI is a viable MBr alternative for fresh market tomato production to retain effective doses of fumigant, manage yellow nutsedge and maintain yields. Grafting is an effective MBr alternative to manage bacterial wilt and root-knot nematode and maintain tomato yields. / Ph. D.