This thesis deals with the factors responsible for induction of the formation of tuber potato (Solanum tuberosum L.) in in vitro conditions. The aim was to observe cultivation of the nodal segments of stems on the induction medium with reduced content of inorganic nitrogen 12 umol, 80 g/l sucrose and the addition of 10 mg/l BA for the for-mation of tubers. The frequency of tuberisation was evaluated, as well as morfological changes, size and weight of the tubers. Three groups of explants were established diffe-ring in lenght of cultivation on the induction medium. These were monitored for chan-ges in the content of endogenous ABA in the nodal segments of stem and stolon. Moni-toring of the changes in content of endogenous cytokinin, nitrogen content, production of ethylene, ethane and CO2 was performed as well. Permanent microscopic preparations were prepared to detect transformation of the axillary bud into stolon, resp. tuber. Increased content of ABA during the tuber formation demonstrates its effect on tuberi-zation. Amongs cytokinins, the biggest effect of cytokinins on tuberization has BA, iP and iPR. Conversely, Z and ZR had no influence. Contents of ethylene, ethane,CO2 and nitrogen are related to the lenght of culturing on the induction medium.

While group homes seemed to be the ideal solution for the deinstitutionalization movement started in the 1950s, many group homes face significant challenges regarding staffing, and staff training and performance. Organization behavior management (OBM) may offer some solutions to increasing staff performance. Additionally, choice is a topic rarely researched in OBM and applied behavior analytic (ABA) research. The purpose of this study is not only to create a treatment package that increases data collection among group home staff, but also to examine choice, and whether increased choices related to a behavioral intervention package impacts the efficacy of the treatment package. Participants were divided into two groups. The choice group was able to make four decisions regarding the procedures used in the treatment package, while the no choice group had those decisions imposed upon them in the treatment package. The results of the groups were compared. The four-component treatment package was successful in increasing average weekly data collection from 0% during baseline to over 50% in the four weeks of intervention. The choice group performed higher than the no choice group every week of intervention, showing that the minimal effort needed to take choice into consideration in an intervention would be worthwhile.

Les stress abiotiques sont la cause principale des pertes de rendement agricole dans le monde. Aujourd'hui, le développement d'espèces capables de résister à ces stress est d'une importance majeure, en particulier dans le contexte de la croissance démographique actuelle et du changement climatique mondial. La phytohormone acide abscissique (ABA) contrôle divers processus cellulaires et induit un signal de protection des plantes contre les stress abiotiques. Parmi les différents évènements moléculaires impliqués dans la voie de signalisation de l'ABA, les cascades de signalisation des mitogen activated protein kinase (MAPK) jouent un rôle important dans la transmission du signal. Cependant, seulement un nombre réduit de MAPK ont été identifiées et caractérisées jusqu'à maintenant.J'ai isolé 2 proches homologues MEKK-like MAPKKKs d'Arabidopsis, MAPKKK17 et MAPKKK18, dont le niveau d'expression étaient fortement induit en réponse à l'ABA et aux stress abiotiques. Chez les mutants insensibles à l'ABA, pyr1/pyl1/pyl2/pyl4 et hab1G246D, l'expression ABA- et sel-dépendant de ces deux gènes était fortement réduite, indiquant que ces 2 kinases agissent en aval du complexe de signalisation de l'ABA. L'utilisation de plantes transgéniques exprimant, sous le contrôle de son propre promoteur, le gène MAPKKK18 fusionné à une étiquette PC2 ou YFP a permis de montrer par western blot que la protéine s'accumulait suite à un traitement à l'ABA et non pas en réponse au stress abiotiques. Ces données montrent que l'ABA est le régulateur majeur de la fonction de MAPKKK18.Suite à une approche de yeast-2-hybrid, j'ai pu identifier MKK3 comme la seule MAPKK interagissant avec MAPKKK17 and MAPKKK18. Ces résultats ont pu être confirmés via la technique de BiFC. Dans des protoplastes de mésophylle, il apparait que MAPKKK17 et MAPKKK18 activent MKK3, indiquant que ces deux gènes codent pour des kinases fonctionnelles. Afin d'apporter des preuves génétiques, j'ai isolé les T-DNA knockout mutants de ces 3 gènes. Des analyses de germination révèlent que mkk3-1 est hypersensible à l'ABA, au sel et au mannitol tandis que la lignée de surexpression Gain-de-fonction présente un phénotype opposé. Cependant, les doubles mutants mapkkk17/18 ne présentent pas de phénotype de germination. D'autres analyses ont pu montrer que mkk3-1 est sensible à la sécheresse et au stress salin tandis que les lignées surexpresseures sont plus tolérantes. Le double mutant mapkkk17/18 est quant à lui seulement sensible au NaCl. Pris dans leur ensemble, ces résultats indiquent que MAPKKK17/MAPKKK18 et MKK3 forment un complexe régulant la réponse des plantes aux stress abiotiques selon une voie dépendantes de l'ABA. / Abiotic stresses are the principal cause of crop failure worldwide. Developing crop plants better able to withstand these stresses has assumed great importance especially in the context of current population growth and global climatic change. The phytohormone abscisic acid (ABA) regulates diverse cellular processes and transduces signals to protect plants from abiotic stresses. Among the molecular elements working in ABA signaling, the mitogen activated protein kinase (MAPK) cascades play important roles in regulating the signaling network. To date, however, only a handful of MAPKs have been identified and characterized in ABA signaling. I isolated 2 closely related Arabidopsis MEKK-like MAPKKKs, MAPKKK17 and MAPKKK18, whose transcript expressions were highly induced by ABA and abiotic stresses. In 2 ABA insensitive mutants, pyr1/pyl1/pyl2/pyl4 and hab1G246D, the ABA- and NaCl-dependent expression of MAPKKK17 and MAPKKK18 was strongly reduced, indicating that these 2 kinases act downstream of the core ABA signaling complex. Western blot analysis of transgenic plants that expressed either a PC2 or YFP tagged MAPKKK18 under endogenous promoter revealed that MAPKKK18 protein strongly accumulated in response to ABA treatment but not in response to other abiotic stresses. This data indicated that ABA is the major regulator of MAPKKK18 protein function.Using yeast-2-hybrid approach, I identified MKK3 as the downstream MAPKK interactor of MAPKKK17 and MAPKKK18, and confirmed these interactions via BiFC assays. In mesophyll protoplasts, MAPKKK17 and MAPKKK18 activated MKK3, indicating that these 2 genes encode functional kinases. To provide genetic evidence of their functions, I isolated T-DNA knockout mutants of these genes. Germination assays reveal that mkk3-1 mutant was hypersensitive to ABA, NaCl and Mannitol stress whereas the over-expression line was resistant. The double homozygous mutant of mapkkk17/18 was not affected in germination. Further analysis revealed that mkk3-1 seedlings were sensitive to NaCl and terminal drought whereas the over-expression lines were resistant. The mapkkk17/18 seedlings were susceptible to NaCl but not terminal drought. Taken together, these results suggest that MAPKKK17/MAPKKK18 and MKK3 form complexes to regulate plant responses to abiotic stress in an ABA-dependent manner.

Bakalauro darbo tikslas buvo išsiaiškinti Taikomosios elgesio analizės (toliau – ABA) metodo taikymo veiksmingumą. Remiantis pedagoginės, psichologinės literatūros analize, atskleisti autizmo sindromo mokslinę sampratą; išnagrinėti vaikų, pasižyminčių autizmo sindromu, ugdymo(si) ypatumus. Pravedant vaikų, pasižyminčių autizmo sindromu, veiklos tyrimą, išanalizuoti komunikacijos ir kalbos įgūdžių įgijimo ypatumus, palyginti savarankiškumo lygmens kitimą, dirbant skirtingais metodais. Taip pat įrodyti naudojamų paskatinimų įtaką, dirbant ABA metodu, komunikacijos ir kalbos įgūdžių įgijimo spartai. Tyrime dalyvavo dvidešimt Kauno vaikų abilitacijos centro ugdytinių, pasižyminčių autizmo sindromu ar turinčių autizmo bruožų. Iš jų pagal lytį: keturios mergaitės ir šešiolika berniukų. Keturių tiriamųjų amžiaus vidurkis – 3,6 metai, šešių tiriamųjų – 4 metai, keturių tiriamųjų – 5 metai ir šešių tiriamųjų – 6 metai. Tyrimo rezultatai parodė, kad ikimokyklinio amžiaus vaikų, pasižyminčių autizmo sindromu ar turinčių autizmo bruožų, komunikacijos ir kalbos raida yra sudėtingas procesas, reikalaujantis nuodugnaus vaiko pažinimo, darbo pagal specialiąsias ugdymo programas, specialiųjų metodų ir priemonių taikymo. Rezultatų analizė leidžia teigti, kad, naudojant ABA metodą, komunikacijos ir kalbos įgūdžiai įgyjami sparčiau nei dirbant įprastiniais darbo metodais. ABA metodas lemia aukštesnį komunikacijos ir kalbos įgūdžių savarankiškumo lygmenį, paskatinimų įvairovė daro įtaką... [toliau žr. visą tekstą] / The aim of the paper is to find out the efficiency of the ABA method. Recently there have been more and more theories explaining autism, at the same time new ways and methods of intervention have been discussed and used. One of them – the ABA (Applied Behavioural Analysis) method, with the help of which specialists try to increase intelectual and academic capacity, social and emotional behaviour of children with autism. The teaching programme covers all areas of functioning, but is especially useful in developing speech and communication for autistic children. The tasks of the paper are to reveal the scientific meaning of an autism syndrom, referring to the analysis of pedagogical and psychological literature; also, to analyse the peculiarities of the development of autistic children. Another task is to analyse the peculiarities of acquiring speech and communication skills using the ABA method, to compare the change of independence level applying this method and usual methods working with autistic children. Moreover, we try to reveal the influence of different forms of stimulation on speech and communication.We propose the hypothesis that the application of the ABA method encourages more effective formation of speech and communication skills. 20 autistic children from Kaunas Abilitation Centre participated in the research: 4 girls and 16 boys – four children aged 3,5; six children - 4 years old, four participants aged 5 and six children - 6 years old. According to the age... [to full text]

In spite of its spontaneous and improvised character, the informal sector has provided virtually the only appropriate housing, in terms of the organization of the outdoor space, for the urban poor of the developing countries. Through an analysis of the outdoor spaces in Eziukwu-Aba, a low-income and informal settlement in Aba, Nigeria, this thesis investigates the organization and mode of use of outdoor spaces, in relation to the day-to-day activities of the urban poor. It focuses on the patterns of outdoor spaces, the categories of activities found in them and the periods of time of the occurrence of the activities. / The findings of this study are compared with those of earlier studies, and the broader implications of these findings on contemporary low-income urban housing in the developing countries are briefly outlined. This study posits that for the urban low-income group of the developing countries, (a) usable space takes precedence over aesthetics and permanence and (b) housing and environmental quality in terms of construction standards are of little significance, compared with employment.

Made available in DSpace on 2016-12-08T16:44:47Z (GMT). No. of bitstreams: 1 PGPV14MA141.pdf: 972601 bytes, checksum: 14535d9511c9bee497994f0a47830cb7 (MD5) Previous issue date: 2014-02-17 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / The climate is very importante for grapevine cultivation, directly influencing the phenological stages of the plant,. In recente years, South Brazil has recorded frequente and excessive rains and low temperature range during the maturation period of the vineyards. These conditions have negatively affected the quality of the grape berries, wich have led to a lower coloration of red grapes and wine. Growth regulators have been used in various wine-growing regions of the world in order to oversome the problems of production and minimize problems caused by unfavorable weather conditions,providing higher quality grapes. The abscisic acid among other duties is responsible for the accumulation of pigments in the skin of grpae berries, and is one of geowth regulators that has been studied and reported in many cultivar of grapes and regons in order to clarify how the ABA operates and its effects on the plant. The objective of this study was to evaluate the exogenous application of diferent doses of abscisic acid Cabernet Sauvignon , Isabel and Ruby cultivars produced in Rio Grande do Sul State. The results for the 2012/2103 season show that the application of abscisic acid increases the contente of anthocyanins, total polyphenols and colour intensity in the skin of grape berries and in the wine and grape juice produced / As condições climáticas são muito importantes no cultivo da videira, influenciando diretamente nas fases fenológicas da planta. No Sul do Brasil, em alguns anos, tem se registrado chuvas freqüentes e em excesso no período de maturação, bem como baixa amplitude térmica, prejudicando a qualidade das uvas produzidas, as quais apresentam pouca coloração. Reguladores de crescimento têm sido utilizados em várias regiões vitícolas do mundo, a fim de superar os problemas de produção e minimizar os problemas causados por situações climáticas desfavoráveis, proporcionando uvas com maior qualidade. O ácido abscísico dentre outras funções é responsável pelo acúmulo de pigmentos, e é um destes reguladores de crescimento que vem sendo estudado e avaliado em muitas cultivares de uvas e regiões a fim de esclarecer como o ABA atua e seus efeitos na planta. Assim, o objetivo do presente trabalho foi avaliar a aplicação exógena de diferentes doses de ácido abscísico nas cultivares Cabernet Sauvignon , Isabel e Rubi nos municípios de Bento Gonçalves, Pinto Bandeira, Vacaria e Santana do Livramento no estado do Rio Grande do Sul. Os resultados obtidos na safra de 2012/2013 comprovam que a aplicação de ácido abscísico aumenta o teor de antocianinas, polifenóis e a intensidade de cor nas cascas das uvas e também no vinho e suco produzidos

Orientador: Michel Georges Albert Vincentz / Tese (outorado) - Universidade Estaulal de Campinas, Instituto de Biologia / Made available in DSpace on 2018-08-16T10:23:29Z (GMT). No. of bitstreams: 1 Tomaz_JuarezPires.pdf: 3125737 bytes, checksum: 8b543a90ff7edc4df818dc09a5ea0bc5 (MD5) Previous issue date: 2008 / Resumo: Na planta modelo eudicotiledónea A. thaliana quatro genes para fatores de transcrição do tipo bZIP que são homólogos a Opaco-2 (O2) do milho, uma monocotiledônea, foram identificados. O2 é um regulador chave do metabolismo coordenado de carbono e nitrogênio e da síntese de prolaminas de reserva durante o desenvolvimento da semente. Estes quatro genes, AtbZIP9, o par de parálogos AtbZIP10 e AtbZIP25, e AtbZIP63, o provável ortólogo de O2, formam o Grupo C de genes bZIP de Arabidopsis. Sabe-se que AtbZIP9 provavelmente desempenhe um papel no processo de desenvolvimento do floema, AtbZIP10 está associado com e resposta à estresses, além de, junto com AtbZIP25, participar na regulação de genes de proteínas de reserva na semente e que AtbZIP63 pode estar envolvido com o balanço energético da planta. Para acrescentar novas informações relevantes sobre a função dos bZIPs do Grupo C e, a longo prazo, entender como a função de O2 evoluiu em angiospermas, iniciou-se neste trabalho uma caracterização detalhada da regulação dos membros do Grupo C em resposta a diversos sinais hormônais e a açúcares. Mostramos que apenas as hexoses glicose e manose e o ácido abscísico (ABA) regulam de maneira transiente a expressão dos genes bZIP do Grupo C, sugerindo que eles representam intermediários mediando as respostas a estes sinais. A glicose reprime a expressão de AtbZIP9 e de AtbZIP63 e induz a expressão de AtbZIP25, ABA reprime a expressão de AtbZIP63 e manose reprime a expressão de AtbZIP25 e de AtbZIP63. Em Arabidopsis, a hexoquinase1 (HXK1) é um sensor da glicose que ativa a síntese e sensibilidade ao ABA para inibir o desenvolvimento da plântula em resposta a glicose. Reportamos aqui que as repressões em curto prazo de AtbZIP9 e AtbZIP63 por glicose e de AtbZIP25 e AtbZIP63 por manose estão mediadas por vias de sinalização independentes de HXK1 e envolvem elementos relacionados a ABA. AtbZIP25 apresenta uma indução por glicose dependente de ABI5 e repressão por manose dependente de ABA2 e ABI4. A repressão de AtbZIP63 por glicose envolve uma via dependente de ABA2 e de ABI5 que é reprimida por ABI4. Já a repressão de AtbZIP63 por manose e de AtbZIP9 por glicose estão inseridas em vias independentes de ABA2, ABI4 e ABI5. A dependência diferencial de ABI5 e de ABI4 na regulação por glicose e manose de AtbZIP25 e de AtbZIP63, permite inferir que ambas hexoses atuam através de vias de transdução distintas e enfatiza a importância de manose como sinal metabólico de regulação. Observou-se ainda que ação conjunta de ABA e glicose apresenta um efeito sinérgico na repressão de AtbZIP63, provavelmente refletindo regulações pós-transcricionais da expressão deste gene. Os dados sugerem que AtbZIP63 representa um importante nó da comunicação entre a sinalização por ABA (estresse abiótico) e por glicose (nível energético) permitindo adequar eficientemente a resposta a estresse abiótico que seja compatível com o estado energético da organismo. / Abstract: In the model eudicot organism A. thaliana (Arabidopsis), four genes encoding bZIP transcription regulatory factors that are homologous to the maize Opaque-2 (O2) locus were identified. O2 is a key regulator of the carbon to nitrogen balance and of the prolamine type storage proteins synthesis during seed development. The Arabidopsis genes, AtbZIP9, the two paralogues AtbZIP10 and AtbZIP25 and AtbZIP63, the most probable O2-ortholgue, together form group C bZIP genes. AtbZIP9 is likely to be involved in phloem development while AtbZIP10 is related to stress responses but is also required for the regulation of seed storage protein genes very much like AtbZIP25. Finally, AtbZIP63 seems to be involved in the control of the energetic balance. In order to get new and relevant information about the role of the group C bZIP genes and consequently obtain new insight into the evolution of the O2-related functions in angiosperms, we initiated a detailed characterization of the regulation of group C members in response to hormonal signals and sugars. We show here that two hexoses, glucose and mannose as well as abscisic acid (ABA) are the only signals that transiently modulated the expression of group C bZIP genes, suggesting they are players in the response induced by these signals. While glucose is shown to repress the expression of AtbZIP9 and AtbZIP63 and to induce AtbZIP25 expression ABA is able to repress the expression of AtbZIP63 and mannose represses the expression of AtbZIP25 and AtbZIP63. In Arabidopsis, hexokinase1 (HXK1) is a glucose sensor that may trigger abscisic acid (ABA) synthesis and sensitivity to mediate glucose-induced inhibition of seedling development. We report that the short term regulation of the expression of AtbZIP9, AtbZIP63 by glucose and the repression of AtbZIP25 and AtbZIP63 by mannose are HXK1-independent and for AtbZIP25 and AtbZIP63, these regulations partly rely on ABA synthesis. It also shown that the activation of AtbZIP25 expression by glucose relies on ABI5 while its repression by mannose appears to be ABA2- and ABI4-dependent. Glucose repression of AtbZIP63 expression seems to involve an ABA2- and ABI5-dependent pathway which is repressed by ABI4. We also reveal that the regulations of AtbZIP63 by mannose and of AtbZIP9 by glucose do not require ABA, ABI4 or ABI5. The differential dependence of glucose and manose-induced regulation of AtbZIP63 and AtbZIP25 expression for ABI5 and ABI4 indicates that both hexoses act through distinct transduction pathways and highlights the importance of mannose as a regulatory metabolite. A synergetic repression of AtbZIP63 by ABA and glucose, which possibly reflects a post-transciptional regulatory scheme of AtbZIP63 expression, was uncovered. Together, the data suggests that AtbZIP63 is a key nod of the ABA (abiotic stress) and glucose (energetic balance) crosstalk network allowing to efficiently adjust the response to abiotic stresses according to the energetic status of the organism. / Tese (outorado) - Universidade / Genetica Vegetal e Melhoramento / Doutor em Genetica e Biologia Molecular

No description available.

The length of the primary root and the density of lateral roots determine the architecture of the root. In this thesis the effect of NAA, ABA and the NO donor SNP alone as well as the combination of ABA or NAA with SNP on lateral root development was investigated. The interaction between CPTIO, a NO scavenger, and NAA or SNP is also reported. Following preliminary experiments in which it was observed that the aerial part of the seedling influenced LR growth and that there was a possible inhibitory effect of light on cultured root tips, experiments were conducted with excised roots tips in the dark. NAA was shown to have the potential to initiate LRs across a wide concentration gradient with the total number of LRs and initiated lateral root primordia (LRP) remaining constant across the range of concentrations tested. Over the last decade, nitric oxide (NO), a bioactive molecule, has been reported to be involved in the regulation of many biological pathways. The presence of NO in the system provided via sodium nitroprusside (SNP), promoted LRP initiation based on the NAA concentration gradient; but without changing the total LR initiation, that is LRs plus primordia density remained constant along the concentration gradient of NAA. The absence of LR and LRP in the treatments of CPTIO (a NO scavenger) with SNP or NAA suggests that NO regulates LRP initiation triggered by NAA, which is in agreement with the recent paper published after the commencement of this study (Correa-Aragunde et al., 2006). In agreement with previous studies, ABA inhibited lateral root development by reducing LR density and the number of LRs. The experiments with fluridone, an ABA biosynthesis inhibitor, may indicate that endogenous ABA was at sufficient concentrations in the excised root tips to inhibit primordia initiation. In this study, evidence is presented for the first time to show that SNP can relieve the inhibitory effect of ABA on LR density and number of LRs suggesting the NO, released from SNP, acts downstream of ABA. Overall these data confirm a critical role for NO in LR initiation.

Problemformulering: Vilka förväntningar har de anställdas inför implementeringen av en aktivitetsbaserad arbetsplats Syfte: Få en djupare förståelse av de anställdas förväntningar inför implementeringen av aktivitetsbaserad arbetsplats Metod: Då tidigare forskning saknas om anställdas förväntningar inför implanteringen av en aktivitetsbaserad arbetsplats har denna studie antagit ett induktivt förhållningssätt. För att kunna besvara syftet med vår studie på bästa sätt har vi valt ett intensivt upplägg i form av en fallstudie. Studien utgår från kvalitativa intervjuer med anställda inom en organisation, där organisationen är i processen att övergå till att en bli helt aktivitetsbaserad arbetsplats. Empirisk insamling: Den insamlade empirin består av två ostrukturerade intervjuer, två semistrukturerade intervjuer, samt en slutlig gruppintervju Slutsats: De slutsatser som vi har kommit fram till är att anställda inom vår undersökta organisation har förväntningar att arbetsmiljön i den aktivitetsbaserade arbetsplatsen kommer vara ny, fräsch och anpassad efter de anställdas behov, flexibel och rörlig, högljudd, utan fasta platser vilket gör att de anställda tvingas sitta med kollegor från andra enheter, samt att de ergonomiska förutsättningarna kommer vara sämre. De anställda förväntar sig att arbetssättet kommer påverkas på följande sätt: de anställda får större möjlighet till att arbeta på distans, de kommer att lättare kunna samarbeta över enheter, anställda kommer att störa varandra i större grad, den individuella prestationen kommer att bli sämre, samt att de anställda kommer sitta på samma platser. Den djupare förståelse som vi har fått är att de anställdas förväntningar har en stark koppling till information. Den information som de anställda har fått av organisationen har påverkat deras förväntningar, och således är information en underliggande faktor som går att använda för att styra förväntningar.