A global outbreak of a new strain of H1N1 influenza virus caused the 2009 flu pandemic. Taiwan was one of the 12 countries in the world capable of producing H1N1 vaccine domestically. The domestic vaccine manufactured by Adimmune Corporation proved to be safe and effective. However, the public opinion generally criticized negatively toward this H1N1 prevention work. This study explored the various entities and relations surrounding the prevention. The first experiment looked into people¡¦s recall toward government¡¦s promotion. This experiment used EyeLink to track subjects¡¦ eye movements on the promotional posters. The data showed the correlation between eye movement and recall, as well as the amount of information received by the subjects. The second experiment looked into people¡¦s decision toward H1N1 prevention. This experiment constructed an AHP model to study the problem. The subjects from various fields answered their view toward H1N1 prevention alternatives, and reported the actual inoculation rate of H1N1 vaccine. The goal aimed to choose a prevention method. The criteria included convenience, confidence, safety, and comfort. The alternatives included vaccination, medication, and sanitation. The experiment outcome summarized the inoculation statistics, attention distribution, involvement correlations, and decision priorities. The experiment result shows the subjects do not receive government¡¦s promotion well. The result also shows the subjects prefer sanitation for prevention. In addition, this study investigates Taiwan¡¦s vaccine and disease prevention development status. The analysis examines Adimmune Corporation and the vaccine market from different aspects. Finally, the conclusion provides recommendations for the people, the vaccine factory, and the government.