This diploma work deals with a design of the accumulated chain conveyor with circuit palette. The work is concentrated on the design of workings and functional and strenght caltulations. The drawing dokumentacions are within the range detail drawings and assembly drawings.

The diploma thesis deals with a six-month monitoring and changes of water chemism in tanks of sugar factory in Hrušovany nad Jevišovkou. The monitoring was carried out between 17 April 2018 and 3 October 2018 for 3 sedimentation tanks and 1 accumulation tank. It was carried out outside of the main beet campaign. At the time of sampling from the tanks, values of the following quantities were recorded: pH, temperature, conductivity and transparency of the water. In next 24 hours the total amount of nitrogen, the amount of nitrogen in nitrites, nitrates and ammonia, as well as the total amount of phosphorus, the amount of orthophosphates, chemical oxygen demand, biochemical oxygen demand, acid and base neutralisation capacity, the amount of calcium, iron, chlorides, insolubles and chlorophyll a were determined in the laboratory of fisheries and hydrobiology Department at Mendel University based in Brno. The values of the indicators varied according to the season, the amount of the discharged waste floating and washing water and the temperature. The highest values of the pollution in average were determined for the sedimentation tank n. 1, to which more waste water had been launched in 2017 because of the yield sediment. The highest values there were for the indicators as the total amount of nitrogen 307,9 mg/l, the amount of nitrogen in ammonia 117 mg/l, chemical oxygen demand 11600 mg/l, biochemical oxygen demand 8200 mg/l and the amount of insolubles 1510 mg/l. However, the high values for the sedimentation tanks are not a major problem as they are getting lower during the year due to the digester sludge and its sedimentation. Also important fact is, that the water in the sedimentation tanks is being overflowed to the accumulation tank, where it is diluted and may be adjusted if needed. Then it is possible to drain the purified water from the accumulation tank to the river Jevišovka (IDVT 10100035), but parameters of the water need to meet the limits of Government Regulations n. 401/2015 Sb. If the values are higher than those limits (as it was found for the monitoring described in this diploma thesis), there is a baffle built near the draining mechanism of the accumulation tank, so the water may be adjusted there in a smaller amount. However, the sugar factory in Hrušovany nad Jevišovkou has never drained the water from the accumulation tank to the river Jevišovka, because of the fact that 50 cm of the water column evaporates from all 4 tanks each year. The baffle was built just because of preliminary experiments of effective purifying the waste water, that could be drained to the river in the future. The values of the parameters of the water from the accumulation tank were compared with the results gained in 2010, 2012, 2014 and 2016 in the same 2 months of sampling. The results of those 5 measurements are also compared to the permissible values stated by the Government Regulations n. 401/2015 Sb.

Subject of this masters thesis is the constructional project of the wooden lamelas manipulator. Thesis comprises design of the two possible solution for two main parts of the device, calculational part and constructional solution of the components. Part of the thesis is design documentation and the model of the manipulator.

Doctoral thesis deals with domain of electric energy storage. It seeks to define the methods of accumulation, which can be used in industrial applications and define the conditions for the use of storage systems in electric power systems with extended penetration of renewable energy sources. In the context of current developments in this field is analyzed detail one of the perspective storage systems - Vanadium Redox Battery (VRB). One of the outcomes of this work is economic and energy analysis of storage systems, which are conceived with a disproportion between production and consumption of energy. The work was supported by the Centre for Research and Utilization of Renewable Energy (CVVOZE) no. CZ.1.05/2.1.00/01.0014 and research project no. FEKT S-11-9.

The goal is to design a storage tank to the heat pumps air to water. The first part describes how a heat pump works and why it is advantageous to use the tank. Another section focuses on ways to accumulation of heat. Established a program that simulates the behavior of the heat pump system with storage tank, described how this program works and is designed tank size for a given heat pump. The last chapters deal with the calculation of minimum area of heat flow exchanger and the proposal inlet valve for improving the thermal stratification of the tank.

This diploma thesis deals with analysis of particular elements of ground water storage tanks, their comparison and describtion of their characteristic. Basic information of designing volume of accumulation tank is included in the first part followed by a key section where particular construction parts and elements are described. In the next section, new types of water tanks are mentioned. In the final section of this thesis, study of 5 existing water tanks was accomplished. A technical recommendation is summed up in the final part that can be taken into account when constructing a new water storage tank.

The thesis proposes heating the family log house. It contains a theoretical analysis of thermal insulation and thermal behavior of log house. The work on the proposed experiment termocentral box, who cares about the operation and management of the heating in the house with the possibility of control over the internet. The findings were practiced in the design of heating through termocentral box the heat source for wood, storage tank and preparation hot water.

This diploma thesis is focused on finite element analysis of storage tank. The designed computational model in this work is based on existing storage tank documentation. Diploma thesis is divided into parts. Introduction part consists of various storage tanks types description and basis of finite element method. Next part contains methodical process of computational model designs, its characteristics and purpose. Designed models are loaded with set of specific border conditions. These tests are evaluated and described more closely. After that, the table of results is created and von Misses stress of all models is compared. The best model matching real construction is selected after this compare. The final part of this work consists of suggested construction changes based on the analysis results.

This master`s thesis deals with low-temperature heating that employs a heat pump. The introduction gives reasons for utilization of the renewable heat sources for heating. The next part focuses on a practical application of renewables on the heating of a family house. It covers a detailed description of the building and the designed heating system which includes: the calculation of thermal losses, design of the low-temperature boiler, heat pump, safety devices, heating elements, sizing and control of the system. The last part is devoted to the assessment of the system performance.

Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Simona Suchá Supervisor: RNDr. Jakub Hofman, Ph.D. Title of diploma thesis: Study of ABC drug efflux transporter inhibition by selected tyrosine kinase inhibitors using accumulation methods with cytostatic substrates ATP-binding cassette (ABC) drug efflux transporters are transmembrane proteins that utilize the energy from ATP hydrolysis to drive transport of endogenous and exogenous compounds out of the cell. The overexpression of ABC transporters plays a crucial role in the development of multidrug resistance (MDR), a phenomenon responsible for the failure of chemotherapy. Tyrosine kinase inhibitors (TKI) represent novel beneficial therapeutic approach in cancer treatment. TKI block tyrosine kinases which regulate important cellular processes. Deregulation of these enzymes can lead to various types of cancers. In the present work, we investigated interaction potential of selected TKI (alectinib, brivanib, osimertinib, selumetinib) in MDCKII parent cell line and those transduced with human efflux transporters ABCB1, ABCC1 and ABCG2. Using the accumulation studies, we determined the amount of accumulated model substrates (daunorubicin, mitoxantrone) and evaluated the inhibitory effect of...