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Eutrophication is a major concern in the Baltic Sea and it is affecting macrophyte communities by promoting the growth of opportunistic algae and decreasing the cover of perennial macrophyte species via shading. It is however uncertain how common eutrophication and its symptoms are in the northern parts of the Baltic Sea, the Botnian Sea. The aim of this study was to evaluate if Sörleviken, a bay in the Bothnian Sea, show signs of increased eutrophication pressure in 2020 compared to 2007 based on changes in macrophyte cover and composition. The macrophyte community was inventoried with under-water video techniques in 2020 along three transects, matching transects previously inventoried by a diver in 2007. The three transects were located in the inner, middle and the outer parts of the bay. The results showed that macrophyte diversity was lower in 2020 than in 2007 along the outer transect, but overall, the total cover of macrophytes, relative cover of opportunistic algae, species richness and evenness remained unchanged. A possible higher presence of Stuckenia pectinata (former Potamogeton pectinatus) and a possible lower presence of Chara aspera in 2o2o compared to 2007 might be evidence of higher eutrophication pressure in 2020. However, by observing the general changes in the macrophyte community, this study only provides weak or inconclusive signs of increased eutrophication pressure, thus Sörleviken shows no signs of either improvement of or further increases in eutrophication pressure by 2020 compared to the observations in 2007.

This study aims to account for the potential of algae as future innovative foods in the Swedish market. The methods for collecting the empirical material were quantitative content analysis and mapping of how many food companies there are in the Swedish market that have algae in their products. The second method was a qualitative interview survey in which the respondents were allowed to express their opinions and perceptions about circular design, food technology and algae as alternative foods. Through results and analysis, it has been shown that there are only a couple of food companies with a focus on algae on the Swedish market, but that the market is in its infancy. A lot of research is done on the subject and large investments are made on algae research in Sweden. This in turn shows that there is interest and potential for these innovative and more climate-friendly foods. Unfortunately, it has been difficult to establish innovative food companies, as Sweden is a country that has valued the safety and development of existing foods. On the other hand, Swedes are proving to be the most open consumers to new trends and embrace new foods. This is good for the development of testing new foods in the Swedish market before export. Through the Government's action plan for the 2030 agenda, innovation is a cornerstone that opens up better conditions for food innovation. It is obvious that it is necessary to invest in innovation in the food chain in order for Sweden to be globally competitive and achieve these goals to reach a sustainable future. The global goals that will form this study are the following, 2 - zero hunger, 12 - responsible consumption and production, 13 - climate action, 14 - life below water.

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Copper (Cu) is required by marine microbes for essential biological processes, including photosynthesis and nitrogen fixation, but can be toxic above a certain threshold. Copper bioavailability in seawater is regulated by complexation with dissolved organic ligands of unknown source and structure. Culturing experiments have demonstrated the production of high-affinity Cu-binding ligands by marine algae in response to metal stress or limitation, suggesting they function either as metal ‘sponges’ to reduce copper toxicity or ‘carriers’ that promote uptake. The goal of my thesis research was to develop methods for the recovery and characterization of Cu ligands from seawater that could then be applied to natural samples to investigate sources and structures of recovered ligands. Using natural seawater spiked with model Cu ligands, I developed an immobilized Cu(II)-ion affinity chromatography (Cu(II)-IMAC) protocol which was shown to be effective in quantifying an operationally defined subset of natural Cu ligands. I then applied Cu(II)-IMAC to seawater collected along transects in the Canadian Arctic and NE Pacific Ocean to assess the abundance of this ligand pool across a diverse set of samples. Ligand distribution profiles and their covariance with other components of seawater (e.g. chlorophyll) were consistent with in situ biological production of some Cu-binding ligands. Model ligands spiked into seawater and recovered by Cu(II)-IMAC were also used to develop protocols for structural characterization of Cu ligands by solid-phase extraction (SPE) and tandem mass spectrometry (MS/MS). This research provides new tools for the isolation and characterization of copper ligands in natural samples, and new insights into the biogeochemical cycling and ecological significance of Cu in the ocean. / Graduate

Of the potential feedstocks for biofuels, microalgae is the most promising, and raceway ponds are the most cost-effective method for growing mircoalgal biomass. Nevertheless, biofuel production from algae must be more efficient to be competitive with traditional fuels. Previous studies using arrays of airfoils, triangles, and squares at high angles of attack show an increase in mixing in raceways and can improve productivity by up to a factor of 2.2. Some researchers say increasing mixing increases growth due to the flashing light effect while others claim it is the decrease in the fluid boundary layer of the cells that increases mass transfer. Whatever the reason, increasing growth by increasing mixing is a repeatable effect that is desirable to both reduce operation costs and increase production. An experimental raceway is constructed to test the effect of a delta wing (DW) on raceway hydraulics in the laboratory using fresh-water. The DW is an isosceles triangle made of plate material that is placed at a high angle of attack in the circulating raceway flow. Results from this investigation can be scaled to larger growth facilities use arrays of DWs. Two vortices are found downstream of the DW when used in this way and create significant vertical fluid circulation. Stereo particle image velocimetry (PIV) is used to quantify and optimize the use of delta wings as a means to increase fluid mixing. Stereo PIV gives three components of velocity in a measurement plane at an instant. Three studies are performed to determine the optimal paddle-wheel speed, angle of attack, and DW spacing in the raceway based on mixing. Two new mixing quantities are defined. The first is the Vertical Mixing Index (VMI) that is based on the vertical velocity magnitude, and the second is the Cycle Time required for an algal cell to complete a cycle from the bottom to the top and back again in the raceway. The power required to circulate the flow is considered in all results. The Paddle-wheel Speed Study shows that the VMI is not a function of streamwise velocity, which makes it very useful for comparison. The Cycle Time decreases quickly with streamwise velocity then levels out, revealing a practical speed for operation that is lower than typically used and consumes only half the power. The angle of 40° is optimal from the results of the Angle of Attack Study for both VMI and Cycle Time. The third study is the Vortex Dissipation Study and is used to measure the distance downstream before the vortices dissipate. This information is used to optimize the DW spacing for profit considering the additional costs of adding DWs.

The Logan City Environmental Department operates a facility that consists of 460 acres of fairly shallow lagoons (~ 5'deep) for biological wastewater treatment that meets targets for primary and secondary treatments (solids, biological oxygen demand (BOD), and pathogen removal). Significant natural algal growth occurs in these lagoons, which improves BOD removal through oxygenation and also facilitates N removal through volatilization as ammonia under high pH conditions created by algal growth. Phosphorus, however, is non-volatile and stays in the water and likely cycles in and out of algal cells as they grow and die in the lagoons. In the near future, the regulatory limits on phosphorus released from the Logan wastewater treatment facility are likely to become significantly lower to counter potential downstream eutrophication. One way to potentially lower phosphorus levels in the wastewater effluent is through management of algal growth in the lagoons. As mentioned above, algae growth naturally occurs in the treatment lagoons and if the algal biomass is harvested when growth yields are highest, the phosphorus contained in the cells could be removed to obtain phosphorus-free water. The algal biomass could then be used for production of biofuels. This research focuses on laboratory and pilot assessments to show the ability of algae indigenous to the Logan lagoons to uptake phosphorus and produce biomass that can be used for biofuel production.

Turbulent mixing plays an important role in the distribution of sunlight, carbon dioxide, and nutrients for algae in the raceway ponds. For large-scale raceway ponds the choice of mixing technology still needs to be evaluated in order to prevent algae sedimentation and to enhance light utilization efficiency. In open ponds, mixing the algae culture is of great significance in terms of input energy costs and particularly productivity. A very small amount of research has been performed previously using different vortex generators in the algal raceway ponds, but the quantification of mixing depth relationships is not defined well. By accepting the premise from the literature review that mixing increases algal production, delta wings were selected to study mixing characteristics in the raceway. The main objective of this research was to study algae-raceway hydrodynamics with an emphasis on increasing vertical mixing. A clear acrylic raceway was designed and constructed for flow visualization studies. Experimental investigations were performed to quantify the vertical mixing with and without delta wings in a lab-scale raceway at approximately the same power input to the paddle wheel. Velocity vector profiles and turbulence parameters were measured using an Acoustic Doppler Velocimeter (ADV) at various locations along the entire length of the raceway. The results indicated that the addition of delta wings increases the vertical mixing intensity or circulation of algae cells over the raceway depth. Vortices were observed in the raceway up to a distance of around 3 m downstream of the delta wing. This sort of systematic vertical mixing plays an important role to produce the flashing light effect (light-dark cycles) on algae mass culture. In addition, turbulence dissipation rates were evaluated to compare them with the published literature and to estimate the microscales using the Kolmogorov hypothesis. Also, an energy model was developed to operate the paddlewheel-driven raceway with the delta wing.

The nature of the action of penicillin on the cell walls of bacteria is reviewed. The composition of bacterial cell walls is compared to cell walls of blue-green algae. The test organisms used were thermophilic: Anacystis nidulans grew optimally at 35⁰C, whereas Synechococcus lividus grew best at 45⁰ C. Growth was recorded by reading optical densities. Cells of these two thermophilic blue-green algae were treated with varying dosages of penicillin and streptomycin. Penicillin inhibited growth of Anacystis nidulans and Synechococcus lividus in concentrations of 3.0 Mg/ml and 0.03 Mg/ml respectively. However, when 0.3 Mg/ml of penicillin was added to Anacystis nidulans, an initial lag phase of growth was observed. Possible reasons for this lag are suggested and results of pertinent experiments are discussed. No similar lag was noted in Synechococcus lividus. Streptomycin inhibited growth of both species when present in 0.03 Mg/ml.