Made available in DSpace on 2014-08-13T14:50:40Z (GMT). No. of bitstreams: 0 Previous issue date: 2013Bitstream added on 2014-08-13T18:01:28Z : No. of bitstreams: 1 000747189.pdf: 1571276 bytes, checksum: cb0e6f84e4a758217e41fa9ef75dba98 (MD5) / Changes in the Pentraxin 3 (PTX3) expression throughout pregnancy are not well established but in pregnancy complications higher expression have been described. To evaluate expression of PTX3 in amniotic fluid (AF) throughout the last weeks of normal pregnancies and in pregnancies complicated by preterm delivery (PTD). A crosssectional study was conducted with 120 pregnant women in term and PTD. Samples of AF from all patients were obtained during cesarean section. Amniochorion membranes were collected to localization of PTX3 using immunohistochemistry. Regarding term pregnancies, PTX3 expression increased slightly throughout weeks, but these expressions are not statistically different. Among preterm pregnancies, those with preterm labor (PL) had a higher PTX3 levels than those not in labor and there was a risk increased of 1% in the occurrence of PL when there was a rise of 1 pg/mL of PTX3. Amniotic epithelial, chorionic and decidual cells presented immunoexpression of PTX3. PTX3 is a physiologic constituent of the AF, and its expression is elevated in the presence of spontaneous PL suggesting that PTX3 play a role in the innate immune response during the gestational complications that are related with an infectious/inflammatory conditions. The amniochorion membranes are sources of PTX3

A Ceratoconjuntivite Seca (KCS Keratoconjunctivitis Sicca) é uma desordem imunomediada e resulta de alterações do componente aquoso do filme lacrimal e da deficiência dos componentes lipídicos e mucoso.Seu diagnóstico é baseado no Teste Lacrimal de Schirmer (TLS) e no Teste de Ruptura do Filme Lacrimal (TRFL) e tem como sinais clínicos: secreção mucopurulenta, hiperemia conjuntival, blefaroespasmos, fotofobia, incômodo, dor, vascularização, opacidade corneana e pigmentação, além de cegueira em casos avançados. O tratamento convencional consiste em aplicações diárias de Ciclosporina 0,2% ou Tacrolimus 0,03% (pomada ou colírio oftálmicos), que apesar de controlar a doença, são custosos, não curativos e exigem alto comprometimento da interação paciente-proprietário. A terapia celular usando células-tronco (CT) traz uma nova esperança para doenças sem tratamento efetivo. Neste trabalho utilizamos CT mesenquimais (CTM) obtidas a partir de membrana amniótica (CTMA) de cães obtidas a partir do descarte destes tecidos em campanhas de castrações em diferentes tempos gestacionais, sem formação tumoral quando submetidas ao teste tumorigênico durante 60 dias. Dois animais com KCS crônica foram tratados com duas injeções de CTMA com intervalo de 30 dias, sendo a primeira de 0,5x106 células e a segunda de 1x106 células em cada glândula. Na segunda semana após a terapia foi observado aumento da TLS sugerindo um benéficio da terapia que foi diminuindo com o passar das semanas. O TRFL oscilou durante os testes e não apresentou diferenças significativas. A terapia celular utilizando CTMA de cães melhorou a condição ocular nos dois casos em momentos e parâmetros variados, com repercussão na melhoria da superfície, mas não houve regressão do quadro clínico. Investigações futuras em estágios menos avançados da doença podem ajudar a elucidar os mecanismos pelos quais esse efeito foi obtido / Keratoconjunctivitis Sicca (KCS) is an immune-mediated disorder and results from changes in the aqueous component of the tear film and also from the impairment of lipid and mucous components. Diagnosis is based on Lacrimal Schirmer Test (LST) and Tear Film Break Test (TFBT) and its clinical signs: purulent discharge, conjunctival hyperemia, blefaroespasmos, photophobia, discomfort, pain, vascularization, corneal opacity and pigmentation, addition of blindness in advanced cases. The standard treatment consists of daily applications of 0.2% cyclosporin or tacrolimus 0.03% (ophthalmic ointment or drops), that in spite of controlling the disease are expensive, require the patient does not curative and and require greater involvement of the patient-owner interaction. Cell therapy using stem cells (SC) brings new hope for diseases without effective treatment. In this work we used CT mesenchymal cells (MSCs) obtained from dogs amniotic membrane (AMSC) in the capanhas of castration at different times gestacionai without tumor formation tumorigenic when subjected to the test during 60 days. Two animals with chronic KCS were treated with two injections of CTMA with 30-day intervals, the first of which 0,5x106 cells and the second of 1x106 cells in each gland. In the second week after therapy was an increase in LST that has been decreasing over the weeks. The BTTF fluctuated during testing and no significant differences. Cell therapy using AMSC dogs improved the eye condition in both cases at times and different parameters, with consequent improvement in ocular surface but there was no regression of the clinical picture. Future research in less advanced stages of the disease may help to elucidate the mechanisms by which this effect was obtained

Orientador: Márcia Guimarães da Silva / Coorientador: Rodrigo Paupério Soares de Camargo / Banca: Gisele Alborghetti Nai / Banca: Denise Fecchio / Resumo: Não disponível / Abstract: Changes in the Pentraxin 3 (PTX3) expression throughout pregnancy are not well established but in pregnancy complications higher expression have been described. To evaluate expression of PTX3 in amniotic fluid (AF) throughout the last weeks of normal pregnancies and in pregnancies complicated by preterm delivery (PTD). A crosssectional study was conducted with 120 pregnant women in term and PTD. Samples of AF from all patients were obtained during cesarean section. Amniochorion membranes were collected to localization of PTX3 using immunohistochemistry. Regarding term pregnancies, PTX3 expression increased slightly throughout weeks, but these expressions are not statistically different. Among preterm pregnancies, those with preterm labor (PL) had a higher PTX3 levels than those not in labor and there was a risk increased of 1% in the occurrence of PL when there was a rise of 1 pg/mL of PTX3. Amniotic epithelial, chorionic and decidual cells presented immunoexpression of PTX3. PTX3 is a physiologic constituent of the AF, and its expression is elevated in the presence of spontaneous PL suggesting that PTX3 play a role in the innate immune response during the gestational complications that are related with an infectious/inflammatory conditions. The amniochorion membranes are sources of PTX3 / Mestre

Resumo: Determinar o efeito da hidratação oral materna com solução isotônica e água no índice de líquido amniótico (ILA) em gestantes com normodramnia. Sujeitos e métodos: Noventa e nove gestantes com ILA normal, e entre 33a e 36a semana de gestação foram randomizadas em três grupos: hidratação com solução isotônica (n=34), hidratação com água (n=30) e controle (n=35). As mulheres do grupo hidratação com solução isotônica e com água ingeriram 1,5L da solução correspondente e as do grupo controle ingeriram 200 mL de água durante o mesmo período. O ILA foi aferido antes e depois da hidratação e comparados entre os grupos. O ultra-sonografista desconhecia o grupo a que a gestante pertencia. Resultados: A diferença do ILA pós e pré-tratamento foi maior nos grupos solução isotônica e água (12.1 mm, p=0.02; 13.1 mm, p=0.05), quando comparado ao grupo controle (1.4 mm, p=0.74). Não houve diferença significativa entre os grupos com solução isotônica e água. A administração de solução isotônica e água aumentaram a chance de incremento de 20 % do ILA, em 10.2 e 6.0 respectivamente. Conclusão: A hidratação oral materna com solução isotônica ou com água aumentou o ILA em aproximadamente 8.5 % em gestantes com normodramnia. / Abstract: To determine the effect of maternal oral hydration with isotonic solution and water on Amniotic fluid index (AFI) in pregnant mothers with normohydramnios. Subjects and Methods: Ninety-nine pregnant women with normal AFI between the 33rd and 36th week of gestation were randomly divided into three groups: hydrated with isotonic solution (n=34), hydrated with water (n=30), and controls (n=35). The isotonic solution and water groups ingested 1.5L of liquid, and the controls ingested 200mL of water over the same period. AFI was compared before and after hydration and between groups. The ultrasound technician was blinded to which group a subject belonged. Results: The difference in pre and post treatment AFI was higher in the isotonic solution and water groups (12.1mm, p=0.02; 13.1mm, p=0.05) than the controls (1.4mm, p=0.74). There was no significant difference between the isotonic solution and water groups. Administering isotonic solution and water improved the chance of a 20% increase in AFI in 10.2 and 6.0 respectively. Conclusion: Maternal oral hydration with isotonic solution or water increases AFI by approximately 8.5% in pregnant women with normohydramnios. / Orientador: Vera Therezinha Medeiros Borges / Coorientador: Marilza Vieira Cunha Rudge / Banca: Francisco Mauad Filho / Banca: José Carlos Peraçoli / Mestre

Para a avaliação da relação entre o controle glicêmico e o volume de líquido amniótico, foram estudadas sessenta gestações em mulheres com diabetes pré-existente. Para isso, adotou-se a análise da relação entre o índice de líquido amniótico (ILA), avaliado semanalmente a partir de 27 semanas, e o perfil glicêmico (média glicêmica e percentual de valores anormais) obtido na semana anterior a realização do exame ultra-sonográfico. As 659 correlações obtidas foram analisadas por meio de gráficos e do teste de Spearman. Não foi verificada, desta forma, relação entre o ILA e o controle glicêmico / To examine the association between glycemic control and amniotic fluid volume sixty pregnancies with pre-existing diabetes were examined. Amniotic fluid index (AFI) was evaluated weekly after 27 weeks of gestation and self-monitoring blood glucose levels (expressed as mean blood glucose levels and percentage of abnormal values) were recorded the week before ultrasound examination. Six hundred fifty nine examinations were recorded and analysed by graphs and Spearman correlation test. There was no significant association between AFI and glycemic control

Made available in DSpace on 2014-06-11T19:27:44Z (GMT). No. of bitstreams: 0 Previous issue date: 2007-02-26Bitstream added on 2014-06-13T18:56:25Z : No. of bitstreams: 1 rososchansky_j_me_botfm_prot.pdf: 1947576 bytes, checksum: 929aea29e1f474a65eb0cbd2df6026c9 (MD5) / Financiadora de Estudos e Projetos (FINEP) / Determinar o efeito da hidratação oral materna com solução isotônica e água no índice de líquido amniótico (ILA) em gestantes com normodramnia. Sujeitos e métodos: Noventa e nove gestantes com ILA normal, e entre 33a e 36a semana de gestação foram randomizadas em três grupos: hidratação com solução isotônica (n=34), hidratação com água (n=30) e controle (n=35). As mulheres do grupo hidratação com solução isotônica e com água ingeriram 1,5L da solução correspondente e as do grupo controle ingeriram 200 mL de água durante o mesmo período. O ILA foi aferido antes e depois da hidratação e comparados entre os grupos. O ultra-sonografista desconhecia o grupo a que a gestante pertencia. Resultados: A diferença do ILA pós e pré-tratamento foi maior nos grupos solução isotônica e água (12.1 mm, p=0.02; 13.1 mm, p=0.05), quando comparado ao grupo controle (1.4 mm, p=0.74). Não houve diferença significativa entre os grupos com solução isotônica e água. A administração de solução isotônica e água aumentaram a chance de incremento de 20 % do ILA, em 10.2 e 6.0 respectivamente. Conclusão: A hidratação oral materna com solução isotônica ou com água aumentou o ILA em aproximadamente 8.5 % em gestantes com normodramnia. / To determine the effect of maternal oral hydration with isotonic solution and water on Amniotic fluid index (AFI) in pregnant mothers with normohydramnios. Subjects and Methods: Ninety-nine pregnant women with normal AFI between the 33rd and 36th week of gestation were randomly divided into three groups: hydrated with isotonic solution (n=34), hydrated with water (n=30), and controls (n=35). The isotonic solution and water groups ingested 1.5L of liquid, and the controls ingested 200mL of water over the same period. AFI was compared before and after hydration and between groups. The ultrasound technician was blinded to which group a subject belonged. Results: The difference in pre and post treatment AFI was higher in the isotonic solution and water groups (12.1mm, p=0.02; 13.1mm, p=0.05) than the controls (1.4mm, p=0.74). There was no significant difference between the isotonic solution and water groups. Administering isotonic solution and water improved the chance of a 20% increase in AFI in 10.2 and 6.0 respectively. Conclusion: Maternal oral hydration with isotonic solution or water increases AFI by approximately 8.5% in pregnant women with normohydramnios.

Made available in DSpace on 2014-06-11T19:27:56Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-02-28Bitstream added on 2014-06-13T20:57:18Z : No. of bitstreams: 1 ramos_bra_me_botfm.pdf: 447116 bytes, checksum: 446e118d63a97d1a69658b7e651b69aa (MD5) / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Microbial invasion of the amniotic cavity has been described in term deliveries and its role on the immune modulation is of interest to the better understanding of the underlying labor processes. The aim of this study was to determine the prevalence of Mycoplasma hominis and Ureaplasma urealyticum in the amniotic fluid of term pregnancies and to evaluate its influence on cytokines production at the end of pregnancy. A cross sectional study was conducted with fifty five pregnant women out of labor with intact membranes and gestational age between 37 and 41 weeks seen at the Bom Jesus hospital in Ariquemes, Rondônia, between June 2009 and May 2010. Amniotic fluid samples and fragments of chorioamniotic membranes were collected at cesarean section. M. hominis and U. urealyticum detection was performed by PCR and Interleukin (IL)-1β, IL-6, IL-8, IL-10 and Tumor Necrosis Factor (TNF)- levels were determined by ELISA. Chorioamniotic membranes were submitted to histopatological analyses. Presence of M. hominis was detected in 36.4% of amniotic fluid samples and any of them was positive for U. urealyticum. Regarding cytokines levels, 63.6% and 90.9% of samples have not shown detectable concentrations of TNF- and IL-1β. The median concentration of IL-6 and IL-8 were 107.9 pg/mL (0-517.1) and 208.1 pg/mL (0-1897.4), respectively. Interleukin-1, IL-6, IL-8 and TNF- concentrations were not associated with the presence of M. hominis in amniotic fluid, regardless the gestational age. No sample had detectable IL-10 levels. The histopatological analyses have shown no chorioamnionitis in any of the membranes, only a discreet mononuclear infiltration in the decidua could be observed in 40.4% of the samples. Presence of M. hominis was detected in 36.4% of amniotic fluid samples and any of them was positive for U. urealyticum. Regarding cytokines levels, 63.6% and 90.9% of samples have not... (Complete abstact click electronic access below)

Orientador: Nereu Carlos Prestes / Banca: Maria Denise Lopes / Banca: João Carlos Pinheiro Ferreira / Banca: Márcia Guimarães da Silva / Banca: Daniel Bartole de Sousa / Resumo: Tendo em vista, a incidência de problemas fetais observados no momento do parto de animais produzidos in vitro (PIV) e a escassez de literatura nesta área, o presente estudo teve por objetivo determinar os níveis de fator de necrose tumoral-alfa (TNF-), interferon-gama (IFN-), interleucina-6 (IL-6) e IL-8 no fluido amniótico de bezerros oriundos de PIV comparando com aqueles obtidos pela transferência de embrião convencional e inseminação artificial no momento do parto, na tentativa de auxiliar na resolução de problemas ainda pouco conhecidos que são observados a campo durante emprego comercial da PIV. Foram utilizados sessenta animais divididos em grupo 01: vinte vacas gestando bezerros da raça Nelore PO de inovulações de embriões oriundos de doadoras superovuladas pelo método convencional (TE); grupo 02: vinte vacas gestando bezerros Nelore PO de inovulações de embriões oriundos de PIV; grupo 03: vinte vacas Nelore PO gestando bezerros oriundos de inseminação artificial (IA) - controle. Todos os partos dos animais do experimento foram observados. Durante a fase de expulsão e após a ruptura do alantocórion, foi realizada a punção do âmnion para a colheita de 15mL de líquido amniótico utilizando-se agulhas e seringas descartáveis compatíveis. O material foi depositado em tubo plástico e congelado em freezer para posterior análise laboratorial. O acompanhamento do padrão de desenvolvimento dos bezerros no periparto foi realizado. Após a descongelação das amostras foi realizado ensaio imunoenzimático (ELISA) para a dosagem de TNF-, com emprego do Kit comercial: ESS0011 - Bovine TNF Alpha, para IFN- foi Kit comercial: ESS0026 - Bovine IFN-, para IL-6 foi Kit comercial: ESS0029 - Bovine IL-6 da Pierce Biotechnology e para IL-8 Kit... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: In view, the incidence of fetal problems seen at the time of birth of animals in vitro production and the be short of the literature in this area, this study aimed to determine the levels of tumor necrosis factor (TNF-), interferon-gamma (IFN-), interleukin-6 (IL-6) and IL-8 in amniotic fluid of calves from in vitro production (IVP) compared with those obtained by conventional embryo transfer and artificial insemination at the delivery, in an attempt to assist in solving problems that are still little known observed in simulations and the experimental field of commercial employment during IVP. Sixty animals used were divided into groups: 1 - Twenty Nelore cross-breed cows pregnant with Nelore calves of embryos transfer conventional method (ET); 2 - Twenty Nelore cross-breed cows pregnant with Nelore calves obtained by in vitro production after follicular aspiration; 3 - Twenty pregnant Nelore cows bearing calves obtained by artificial insemination (AI). All births of the animals of the experiment were observed. During the expulsion phase the amnion was punctured and 15mL of fluid were collected using a needle and syringes compatible. The material was deposited on plastic tube and frozen in freezer for later analysis. Monitoring the pattern of development of the calves in peripartum was performed. The cytokines levels were measured by immunoenzymatic assay (ELISA). The commercial kit used was: ESS0011 - Bovine TNF Alpha, ESS0026 - Bovine IFN- gamma, ESS0029 - Bovine IL-6 Pierce Biotechnology and comercial Kit: D8000C - Human IL-8 of the R & D Systems. The protocol was performed according to the manufacturer's recommendations. The densities were evaluated in automatic optical reader ELISA. The well plates were read at 450nm. The Kruskal-Wallis Test was used for statistical analysis... (Complete abstract click electronic access below) / Mestre

Submitted by (edna.saturno@ufrpe.br) on 2016-08-11T11:55:17Z No. of bitstreams: 1 Arthur Nascimento.pdf: 397417 bytes, checksum: 40f7d9ee2e954be3d6ecb575b4fe1359 (MD5) / Made available in DSpace on 2016-08-11T11:55:17Z (GMT). No. of bitstreams: 1 Arthur Nascimento.pdf: 397417 bytes, checksum: 40f7d9ee2e954be3d6ecb575b4fe1359 (MD5) Previous issue date: 2008-02-11 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / In order to achieve the identification of sex in fetal sheep, were subjected to the technique of Polymerase Chain Reaction (PCR), 45 samples of DNA extracted from amniotic fluid of pregnant uterus, 24 samples of males and 21 females. The amniotic fluid was obtained by amniocentesis guided by ultrasound for greater accuracy in aspiration and also for subsequent application of the technique in vivo, as simulation. Gestational age was laid on the measurement of fetuses with caliper, which showed variation of 3.3 to 46.5 cm. The samples were divided into three groups based in Kadu and Kaikini (1987): Group 1 (zero to 8.6 cm) from conception to the 60th day of gestation, group 2 (8.7 to 26 cm) among 61th and 105th day, and Group 3 (26,1 cm on) 106th day until the birth. The DNA was extracted using the method phenol-chloroform, obtaining a quantity of less than 50 ng per μL, quantified by comparing through - lambda and visualized in 1% agarose gel. For amplification of genetic material, were used "primers" to the SRY gene (116pb), found in the Y chromosome present only in males, and Aml-X (300pb), autosomal, present in both sexes. In all samples was possible to sex the fetuses and the primers used shown to be quite effective. The sex was confirmed by morphological analysis of fetuses, aside from the concept who genital tubercle still had not moved, proving the effectiveness of the PCR molecular tool for this examination. / Com o objetivo de realizar a identificação do sexo fetal em ovinos, foram submetidas à técnica de Reação em Cadeia da Polimerase (PCR), 45 amostras de DNA extraídas do líquido amniótico de úteros prenhes, sendo 24 amostras de machos e 21, fêmeas. O fluido amniótico foi obtido por amniocentese guiada por ultra-sonografia para maior precisão na aspiração, como também para posterior aplicação in vivo da técnica, com efeito de simulação. A idade gestacional foi estipulada a partir da medição dos fetos com paquímetro, a qual apresentou variação de 3,3 a 46,5 cm. As amostras foram divididas em três grupos segundo Kadu e Kaikini (1987): Grupo 1 (zero a 8,6cm) da concepção ao 60o dia de gestação, Grupo 2 (8,7 a 26 cm) entre o 61o e o 105o dia e Grupo 3 (26,1 cm em diante) 106o até o nascimento. O DNA foi extraído através do método fenol-clorofórmio, obtendo-se uma quantidade inferior a 50 ngpor μl ao ser quantificado por comparação através de -lambda e visualizado em gel de agarose 1%. Na amplificação do material genético, foram utilizados os “primers” referentes aos genes SRY (116pb), encontrado no cromossomo Y presente apenas em machos, e Aml-X (300pb), de caráter autossômico, presente em ambos os sexos. Em todas as amostras foi possível realizar a sexagem fetal e os primers utilizados demonstraram ser bastante eficientes. O sexo foi confirmado pela análise morfológica dos fetos, com exceção do concepto cujo tubérculo genital ainda não havia migrado, comprovando a eficácia da ferramenta molecular PCR para este exame.

A membrana amniótica é uma membrana translucida sendo a membrana mais interna da cavidade amniótica, formada por uma monocamada de células epiteliais disposta sobre uma membrana basal. Com o crescente interesse na utilização de células-tronco provenientes de anexos fetais, esta se torna uma promissora fonte de células-tronco. Sendo assim em trabalho anterior realizado pelo nosso grupo tivemos como objetivo, o estabelecimento da cultura celular e caracterização das células-tronco fetais de membrana amniótica de cão para verificar se a mesma pudesse ser uma nova fonte celular a ser usada nos protocolos de terapia celular, uma vez que os cães têm sido considerados modelos animais atraentes para avaliar novas drogas ou realizar ensaios pré-clínicos. As células de membrana amniótica obtidas a partir do trabalho anterior foram caracterizadas in vitro, observando-se características semelhantes a outras células-tronco mesenquimais. Porém, quando foi analisado o seu o seu potencial carcinogênico observamos a formação de um tumor de crescimento rápido, aproximadamente um mês, após o inóculo dessas células em 10 camundongos imunossuprimidos nude, sendo o tumor identificado histologicamente como um carcinoma embrionário. Diante deste comportamento acreditamos ser de extrema importância analisar células provenientes de novas coletas verificando se a mesmas podem se comportar como as anteriormente estudadas. Com isso, temos como objetivo deste trabalho estabelecer e caracterizar as células provenientes de duas novas coletas em diferentes períodos gestacionais visando verificar se estas células se comportam da mesma que as anteriores isso é se quando inoculadas nos animais formam tumor e assim poder ter certeza de que essas células são ou não, boas alternativas para terapia celular. As células obtidas nestas novas coletas têm características de células-tronco mesenquimais expressando alguns marcadores, tem curva de crescimento semelhante às células-tronco mesenquimais, se aderem ao plástico e se diferenciaram em adipócitos. Diferentemente das células obtidas no estudo anterior estas células não geraram tumor quando injetadas em camundongos imunossuprimidos, nude em até 60 dias após inoculação. / Amniotic membrane is a membrane translucent with the inner membrane of the amniotic cavity, formed by a monolayer of epithelial cells disposed on a basal membrane. With the growing interest in the use of stem cells from fetal membranes, this becomes a promising source of stem cells. So in a previous study conducted by our group we aim, the establishment of cell culture and characterization of fetal stem cells from amniotic membrane from dog to see if it could be a new source cell to be used in cell therapy protocols, Once the dogs have been considered attractive animal models to evaluate new drugs or performing pre-clinical tests. The cells from amniotic membrane obtained from previous work were characterized in vitro, observing characteristics similar to other mesenchymal stem cells. But when it was analyzed its its carcinogenic potential observed the formation of a fast-growing tumor, approximately one month after inoculation of these cells into immunocompromised nude mice 10, and the tumor identified histologically as embryonal carcinoma. Given this behavior we believe is extremely important to analyze cells from new collections by checking if the same can behave like those previously studied. With this, we aim of this work to establish and characterize the cells from two new collections at different gestational periods to verify whether these cells behave the same as the previous ones is that when inoculated into animals form tumor and thus be able to make sure that these cells are either not good alternatives for cell therapy. The cells obtained in these new collections has characteristics of mesenchymal stem cells expressing some markers, growth curve is similar to mesenchymal stem cells, adhere to plastic and differentiated into adipocytes. Unlike the cells obtained in the previous study these cells did not generate tumors when injected into immunocompromised mice, nude within 60 days after inoculation.