Immobilization of photosynthetic cultures has been used to generate biofuels and high value compounds through direct conversion of CO2 and water using sunlight. Compared with suspended cultures, immobilized bacteria can achieve much higher densities resulting in greater areal productivity. Limitations exist however, on the density that can be reached without compromising access to light and other nutrients. In this thesis an optofluidic approach to overcoming the challenge of light delivery to high density cultures of cyanobacteria is described and proof of concept experiments presented. This approach uses optical waveguides to deliver light to cells through bacterial interaction with the evanescent field and is tailored to meet each cell's need for light and nutrients. Experiments presented here demonstrate biofilm proliferation in the presence of evanescent fields. Illumination of surfaces by surface plasmon enhanced evanescent fields is also shown to be an effective and potentially useful technique to grow biofilms within optofluidic architectures.

The limitations of renewable energy technologies require that pathways are carefully chosen such that renewable resources are used most effectively in addressing modern energy challenges. Optimized decision-making is particularly challenging for the forest bioenergy sector because of the multitude of potential pathways and because profit is highly sensitive to biomass procurement costs. I assessed energy wood recovery and procurement costs during semi-mechanized selection operations in the tolerant hardwood forests of Ontario. Logging contractors were able to recover unmerchantable sections of branches normally discarded during conventional operations, amounting to 1.3 to 2.7 dry tonnes of additional biomass per hectare. Supply chain scenarios are used to show that the biomass can be brought to market at a cost similar to mechanized operations. The need for prioritization of decentralization, efficiency, and integration with the value-added forest sector is discussed with reference to the relative scarcity and high cost of the forest resource.

The limitations of renewable energy technologies require that pathways are carefully chosen such that renewable resources are used most effectively in addressing modern energy challenges. Optimized decision-making is particularly challenging for the forest bioenergy sector because of the multitude of potential pathways and because profit is highly sensitive to biomass procurement costs. I assessed energy wood recovery and procurement costs during semi-mechanized selection operations in the tolerant hardwood forests of Ontario. Logging contractors were able to recover unmerchantable sections of branches normally discarded during conventional operations, amounting to 1.3 to 2.7 dry tonnes of additional biomass per hectare. Supply chain scenarios are used to show that the biomass can be brought to market at a cost similar to mechanized operations. The need for prioritization of decentralization, efficiency, and integration with the value-added forest sector is discussed with reference to the relative scarcity and high cost of the forest resource.

Reports from various institutions claim that garbage management in Rwanda has had diverse effects on both the natural environment and human society. Such claims prompted for an exploratory study to find out an optimal system to handle solid waste in Kigali City. The study considered a literature review and primary data from 400 randomly selected citizens. They were surveyed about their opinions on which system they perceived to be the optimal to handle garbage in Kigali City. The computer software Web-Hipre was used to analyze data on the three systems considered to handle solid waste in Rwanda: briquetting, composting, and incineration.The results indicate briquetting as the optimal alternative to handle solid waste from homesteads and workplaces of Kigali City. Briquetting considers production of solid fuels that may reduce destruction of forests for fuel. Other major reasons for briquetting, highlighted by respondents, include improved kitchen hygiene and sanitation and replacement of charcoal for a less dusty fuel. Economic factors governed surveyed participants to prioritise briquetting system to handle solid waste in Kigali. Composting may be considered for transforming organic materials into mulch to support farming activities in rural areas as well as gardening in the towns. However, a centralised incineration system is presently not suitable. The private sector has so far not fully been engaged in the transformation of solid waste into bioenergy in Rwanda.

Reports from various institutions claim that garbage management in Rwanda has had diverse effectson both the natural environment and human society. Such claims prompted for an exploratory study to find outan optimal system to handle solid waste in Kigali City.The study considered a literature review and primary data from 400 randomly selected citizens. They weresurveyed about their opinions on which system they perceived to be the optimal to handle garbage in KigaliCity. The computer software Web-Hipre was used to analyze data on the three systems considered to handlesolid waste in Rwanda: briquetting, composting, and incineration.The results indicate briquetting as the optimal alternative to handle solid waste from homesteads and workplacesof Kigali City. Briquetting considers production of solid fuels that may reduce destruction of forests for fuel.Other major reasons for briquetting, highlighted by respondents, include improved kitchen hygiene andsanitation and replacement of charcoal for a less dusty fuel.Economic factors governed surveyed participants to prioritise briquetting system to handle solid waste in Kigali.Composting may be considered for transforming organic materials into mulch to support farming activities inrural areas as well as gardening in the towns. However, a centralised incineration system is presently notsuitable. The private sector has so far not fully been engaged in the transformation of solid waste into bioenergyin Rwanda.

Environmental sustainability in Sweden is challenged by rising greenhouse gas emissions from transportation, input-intensive agriculture and potentially unsustainable removal of organic material from forests and farmland. The Swedish government is working towards sixteen environmental quality objectives in order to overcome these problems, among others.  The past decade gave rise to an international research community dedicated to investigate the age-old practice of applying charcoal to soil, practiced most notably by ancient civilisations in the Amazon region of South America. The high level of interest and controversy around this subject inspired this investigation of biochar’s potential benefits in Sweden.  A scientific study was conducted to examine the potential of biochar to enhance environmental sustainability in Sweden. This was largely a desk study, supplemented by expert interviews, GIS map work, an experiment and mathematical analysis.  It was found that there was insufficient research to date to prove the agronomic benefits of biochar in Sweden. More field studies are required to build up the evidence of its potential. Furthermore, as the rate of mineralization of the carbon content is dependent on numerous factors, including the composition of the soil to which the biochar is applied, it is difficult to conclusively define biochar’s carbon storage potential.  This study adds to the existing body of knowledge on the subject by integrating the conclusions from a variety of studies and expert opinions, as well as by providing maps indicating land areas in Sweden that would be likely to benefit from biochar application.

Titel: Träpellets: en växande affärsmöjlighet Produktion av träpellets i Brasilien för export till Europa Nivå:        D-uppsats i ämnet företagsekonomi Författare:             Susanna Tosterud Handledare:                      Jonas Kågström Datum:                           2012 – Juni Syfte:                                                    Syftet med denna studie är att undersöka de ekonomiska förutsättningarna som finns att tillverka pellets i Brasilien för export till Europa. Metod:       Studien har använt en kvalitativ metodansats där datainsamlingen för empiriavsnittet består främst av semistrukturerade öppna intervjufrågor, som skickades till respondenterna via mejl. Sedan skedde kontakt via telefonsamtal för att säkerställa att informationen var korrekt uppfattad. Respondenternas svar redovisas i empirikapitlet och har analyserats och bearbetats med hjälp av två teorier inom marknadsföring, nämligen marknadsföringsmix (de 4 P: na) och SWOT-analys. Resultat & slutsats:                               Resultatet visar att i nuläget är det inte lönsamt att exportera pellets från Brasilien till Europa. Priserna för pellets på den europeiska marknaden är för låga, en höjning med cirka USD 50/ton är nödvändig för en godtagbar lönsamhet. Den brasilianska pelletsproduktionen behöver vidareutvecklas med avseende på kostnadseffektivitet och kvalitetskrav. Detta för att vara konkurrenskraftig på den europeiska marknaden. Förslag till vidare forskning:            Den empiriska delen av studien har baserats enbart på kvalitativa intervjuer. Det vore intressant att följa upp studien med en kvantitativ ansats för att öka trovärdigheten i svaren. Endast två marknadsföringsteorier har använts i studien och det vore betydelsefullt att ta med en PESTLE analys (politiska, ekonomiska, sociologiska, tekniska, juridiska och miljöaspekter). Uppsatsens bidrag:                               Det diskuteras mycket kring klimatfrågor och de olika alternativ som finns för att ersätta fossila bränslen. Studien bidrar till en ökad förståelse av de förutsättningar som krävs för en lönsam pelletsexport från Brasilien till Europa. Nyckelord:                                         Pelletsmarknad, Brasilien, export, marknadsundersökning och bioenergi. / Title:                      Wood pellets: A growing business opportunity Production of Brazilian wood pellets for export to Europe Level: Final assignment for Master in Business Administration Author:                  Susanna Tosterud Supervisor:                Jonas Kågström Date:                      2012 - June Aim:                                The purpose of this study is to examine the existing economic conditions to produce pellets in Brazil for export to Europe. Method:             The study used a qualitative method where data collection for the empirical section consists mainly of semi-structured open interview questions that were sent to the respondents via e-mail. Then they were contacted by phone to make sure that the information had been correctly perceived. The respondent’s answers are reported in the empirical chapter and have been analyzed and processed with the help of two marketing theories: the marketing mix (the 4 Ps) and SWOT analysis. Result & Conclusions:                        The result shows that at present time it is not profitable to export pellets from Brazil to Europe. Prices for pellets in the European market are too low, an increase of approximately USD 50/ton is necessary for an acceptable profit. The Brazilian pellet production needs to be further developed with regards to cost and quality requirements. This is needed in order to become competitive in the European market. Suggestions for future research:      The empirical part of the study was based solely on qualitative interviews. It would be interesting to follow-up this study with a quantitative approach to improve reliability in the answers. Only two marketing theories have been used in this study and it would be important to also make a PESTLE analysis (political, economic, sociological, technological, legal and environmental aspects). Contribution of the thesis:       There are many discussions about climate change and the various options available to replace fossil fuels. This study contributes to a better understanding of the prerequisites for profitable pellet production in Brazil based on exports to Europe. Key words:                                  Pellet market, Brazil, exports, market research and bioenergy.

Hemp is a versatile plant with many uses that has been around for thousands of years. The plant is highly adaptable andwill cultivate in almost any soil. It has excellent remediation properties and has shown to be relatively consistent when itcomes to crop yields. The aim of this literature survey is to examine how hemp can be used to be considered asustainable energy crop in Sweden through database searches and interviews. Hemps characteristics as an energy cropand its remediation properties is evaluated and compared with other plants that are used in Sweden. The result showsthat hemp is a competitive crop when used for solid fuel and that a closed-loop solution is achievable. Further studiesare needed for a closed-loop solution to be possible when using hemp as a biogas crop, mainly for establishing asustainable waste treatment.

Sustainable waste treatment and lignin development strategies targeted for biorefineries will benefit industry, consumers, and the environment. This dissertation demonstrates the feasibility of a novel biochemical pathway capable of converting sugars and lignin sourced from biorefinery waste streams into microbial oils suitable for biodiesel, cosmetic, and biopharmaceutical applications. This biochemical pathway also presents interesting avenues for the commercial production of higher-value intermediate metabolites such as catechol, protocatechuate, pyruvate, and succinate. Alternatively, this dissertation also demonstrates a unique polymerization strategy for lignin that can be adopted towards the production of green polymeric biomaterials. Overall, these strategies jointly present intriguing routes for lignin valorization.

Application of bioengineering technologies for enhanced biological hydrogen production is a promising approach that may play a vital role in sustainable energy. Due to the ability of several naturally occurring microorganisms to generate hydrogen through varying metabolic processes, biological hydrogen has become an attractive alternative energy and fuel source. One area of particular interest is the production of biological hydrogen in organically-rich engineered systems, such as those associated with waste treatment. Despite the potential for high energy yields, hydrogen yields generated by bacteria in waste systems are often limited due to a focus on microbial utilization of organic material towards cellular growth rather than production of biogas. To address this concern and to improve upon current technological applications, metabolic engineering approaches may be applied to known hydrogen producing organisms. However, to successfully modify metabolic pathways, full understanding of metabolic networks involved in expression of microbial traits in hydrogen producing organisms is necessary. Because microbial communities associated with hydrogen production are capable of exhibiting a number of phenotypes, attempts to apply metabolic engineering concepts have been restricted due to limited information regarding complex metabolic processes and regulatory networks involved in expression of microbial traits associated with biohydrogen production. To bridge this gap, this dissertation focuses on identification of phenotype-related biochemical processes within sets of phenotype-expressing organisms. Specifically, through co-development and application of evolutionary genome-scale phenotype-centric comparative network analysis tools, metabolic and cellular components related to three phenotypes (i.e., dark fermentative, hydrogen production and acid tolerance) were identified. The computational tools employed for the systematic elucidation of key phenotype-related genes and subsystems consisted of two complementary methods. The first method, the Network Instance-Based Biased Subgraph Search (NIBBS) algorithm, identified phenotype-related metabolic genes and subsystems through comparative analysis of multiple genome-scale metabolic networks. The second method was the multiple alignments of metabolic pathways for identification of conserved metabolic sub-systems in small sets of phenotype-expressing microorganisms. For both methodologies, key metabolic genes and sub-systems that are likely to be related to hydrogen production and acid-tolerance were identified and hypotheses regarding their role in phenotype expression were generated. In addition, analysis of hydrogen producing enzymes generated by NIBBS revealed the potential interplay, or cross-talk, between metabolic pathways. To identify phenotype-related subnetworks, three complementary approaches were applied to individual, and sets of phenotype-expressing microorganisms. In the first method, the Dense ENriched Subgraph Enumeration (DENSE) algorithm, partial "prior knowledge" about the proteins involved in phenotype-related processes are utilized to identify dense, enriched sets of known phenotype-related proteins in Clostridium acetobutylicum. The second approach utilized a bi-clustering algorithm to identify phenotype-related functional association modules associated with metabolic controls of phenotype-related pathways. Last, through comparison of hundreds of genome-scale networks of functionally associated proteins, the á, â-motifs approach, was applied to identify phenotype-related subsystems. Application of methodologies for identification of subnetworks resulted in detection of regulatory proteins, transporters, and signaling proteins predicted to be related to phenotype-expression. Through analysis of protein interactions, clues to the functional roles and associations of previously uncharacterized proteins were identified (DENSE) and hypotheses regarding potentially important acid-tolerant mechanisms were generated (á, â-motifs). Similar to the NIBBS algorithm, analysis of functional modules predicted by the bi-clustering algorithm suggest cross-talk is occurring between pathways associated with hydrogen production. The ability of these phenotype-centric comparative network analysis tools to identify both known and potentially new biochemical process is important for providing further understanding and insights into metabolic networks and system controls involved in the expression of microbial traits. In particular, identification of phenotype-related metabolic components through a systems approach provides the underlying foundation for the development of improved bioengineering technologies and experimental design for enhanced biological hydrogen production.