The creation of antidrug rules is a part of the whole system of specific primary drug prevention. These rules should make it possible for the school apart from other things to indentify quickly potential drug users among the pupils/students, to find out their parents possibly too and then to offer them goal-directed services within the bonds of a selective and indicative prevention. That{\crq}s why I paid particular attention to the system of antidrug rules in my work. I examined whether the schools had such a system, how clear, transparent and effective it was and which of the rules showed the highest effect. The research was carried out by application of the half-structured dialogue with the methodologists charged with the prevention at secondary schools. I wanted especially to put their practical experience to good use for suggesting new rules and for rethinking the present ones. I chose secondary schools for my research because just the students aged 15 to 19 years have problems with addictive drugs in many cases. They grow up physically and mentally, create new groups, become able and further develop their ability to solve problems, to take decisions, to think abstractly, to create hypotheses and to combine the facts. They become more independent gradually, leave their families and create conditions for the future life. The aim of this work was to evaluate the quality and effectiveness of antidrug measures implemented at chosen secondary schools. The methodologist charged with the prevention should try to involve the complete staff of the school in the programme, everybody should know his role as far as to the prevention and as far as to the solution of situations where the prevention itself does not appear sufficient any more. The schools prefer single prevention campaigns, but the prevention should be comprehensive, long-term and it should embrace all programmes, an individual approach, positive atmosphere at school, effective antidrug rules etc. The use of addictive drugs in the school ambience at the time when the school looks after entrusted persons or keeps them under supervision is a significant intervention in its life. It is therefore necessary to be aware of this phenomenon and to have at disposal an in detail developed crisis plan.

Graduation theses will research the actual attitudes towards problems of drugs, drug dependences and the antidrug preventive programs at schools of teachers {--} the school metodist of primary prevention and teachers of whom the primary prevention is not special part of their work.

Submitted by Elesbão Santiago Neto (neto10uepb@cche.uepb.edu.br) on 2018-05-03T19:20:13Z No. of bitstreams: 1 PDF - Mariana Preta Oliveira de Lyra.pdf: 38679325 bytes, checksum: 88f0e3bf9c14db4acd28916991abc29d (MD5) / Made available in DSpace on 2018-05-03T19:20:13Z (GMT). No. of bitstreams: 1 PDF - Mariana Preta Oliveira de Lyra.pdf: 38679325 bytes, checksum: 88f0e3bf9c14db4acd28916991abc29d (MD5) Previous issue date: 2014-02-07 / CAPES / This study analyzes how the issue of drug trafficking is incorporated into the Union of South American Nations (UNASUR) agenda. The analysis is based on the theoretical framework presented by the Copenhagen School of security studies, specifically Regional Security Complex Theory and (se) Securitization Theory (BUZAN et al, 1998). Thus, there are the following main arguments: South America Regional Security Complex has been going through a period of transition, emerging from a schema without centrality of power to a model centered on a regional power, Brazil; and UNASUR, through three thematic councils (the South American Defense Council, the South American Council for the Global Drug Problem and the South American Council on Citizen Security, Justice and Coordination against the Organized Delinquency), initiated the process of desecuritization of drug trafficking at the regional level. Importantly, the analysis developed in this work is related to the normative framework of UNASUR, since it is a young organization and it is still under construction. Nevertheless, even in the absence of empirical material to harmonize policies and practices, we reaffirm the value of examining the normative level of any international organization. Initially, we conducted a detailed analysis of the ideas of the Copenhagen School, nearing its premises to the South American reality, however, without neglecting its analytical limitations. Regarding the issue of drug trafficking, we drew up an overview of its process of securitization during the Cold War, as well as the detailing of the international drug control regime, indicating the main implementers and supporters of drug policy in the international system. Also, we tried to present the South American reality regarding the drug trafficking, drawing a parallel between the U.S. policy, major external player, and the impact on the management of the issue in South America. In sequence, we analyzed the normative arrangement of UNASUR regarding the theme, highlighting the work performed by the South American Defense Council, the South American Council for the Global Drug Problem and the South American Council on Citizen Security, Justice and Coordination against the Organized Delinquency. Finally, we presented a parallel between intentions and normative of UNASUR and the actions and guidelines of the U.S. drug policy perpetrated during the last 50 years in the region. The Union of South American Nations, therefore, has advanced in discussing the problem of drug trafficking in the region, seeking to expand the approach to the topic while presenting itself as an alternative to the U.S. militaristic model. Thus, we observe that the continuity and consolidation of policies designed by UNASUR may lead to the formation of a South American regime for the global drug problem, which may be more comprehensive and may achieve better results for regional security. / A presente dissertação analisa como a problemática do narcotráfico é incorporada à agenda da União de Nações Sul-Americanas (Unasul). A análise desenvolvida fundamenta-se no arcabouço teórico apresentado pela Escola de Copenhague, mais especificamente a Teoria dos Complexos Regionais de Segurança e a Teoria da (de) Securitização (BUZAN et al, 1998). Dessa forma, têm-se os seguintes argumentos centrais: o Complexo Regional de Segurança da América do Sul passa por um momento de transição, saindo de um esquema sem centralidade de poder para um modelo centrado em uma potência regional, o Brasil; e a Unasul, por meio de três conselhos temáticos (Conselho de Defesa Sul-Americano, Conselho Sul-Americano Sobre o Problema Mundial das Drogas e Conselho Sul-Americano em Matéria de Segurança Cidadã, Justiça e Coordenação contra a Delinquência Transnacional Organizada), iniciou o processo de desecuritização da problemática do tráfico de drogas em nível regional. É importante destacar que a análise desenvolvida nesta dissertação recaiu no âmbito normativo da Unasul, visto que se trata de uma organização jovem e ainda em construção. Entretanto, mesmo na ausência de material empírico que harmonize diretrizes e práticas, reafirma-se o valor do exame do nível normativo de qualquer organização internacional. Inicialmente, conduziu-se uma profunda análise das ideias da Escola de Copenhague, aproximando suas premissas da realidade sul-americana, no entanto, sem negligenciar suas limitações analíticas. No tocante à temática do narcotráfico, traçou-se um panorama do processo de securitização da problemática durante a Guerra Fria, bem como o detalhamento do regime internacional antidrogas, apontando os principais incentivadores e implementadores da política antidrogas no sistema internacional. Também, buscou-se apresentar a realidade sul-americana quanto ao tema, fazendo um paralelo entre a política estadunidense, grande player externo, e o impacto no gerenciamento da questão na América do Sul. Em seguida, analisou-se o arranjo normativo da União de Nações Sul-Americana com relação à problemática, destacando a atuação do Conselho de Defesa Sul-Americano, do Conselho Sul-Americano sobre o Problema Mundial das Drogas e o Conselho Sul-Americano em Matéria de Segurança Cidadã, Justiça e Coordenação contra a Deliquência Organizada Transnacional. Por fim, realizou-se um paralelo entre as intenções e normativas da Unasul com as ações e diretrizes da política antidrogas norte-americana perpetrada nos últimos quase 50 anos na região. A União de Nações Sul-Americanas, portanto, tem avançado nas discussões sobre a problemática do narcotráfico na região, buscando ampliar a abordagem do tema ao mesmo tempo em que se apresenta como uma alternativa ao modelo militarista estadunidense. Dessa forma, vislumbra-se que a continuidade e consolidação das políticas pretendidas pela Unasul podem levar a constituição de um regime sul-americano para o problema mundial das drogas, que seja mais integral e que possa obter melhores resultados para a segurança regional.

Diabetes has, in the past three decades, surged immensely. Because of this, new insulin analogues are constantly in the making.  In clinical studies, the presence of antidrug antibodies can prove a challenge when measuring insulin. In order to overcome the interference from antidrug antibody complexes on the total insulin measurement in human serum, several pre-treatment methods on insulin and polyclonal antibodies spiked samples were tried using ELISA analysis. Several different methods were tried, acid dissociation using a glycine buffer with and without ethanol in different concentrations, high ionic strength dissociation using MgCl2, Polyethylene glycol (PEG) and filtration. The best results were found when using the acid dissociation technique. Using glycine promising results were achieved, especially when 20 % ethanol was added to the acid mixture. Pre-treatment using PEG, MgCl2 and filtration was unsuccessful with the methods used. The main goal was reached through the use of glycine with the addition of 20% ethanol for acid dissociation. The proposed method still leaves significant room for optimisation and needs further verification on real patient samples. However, it is a good step in the direction of a global methodology using ELISA to overcome antidrug antibody interference for total insulin measurement in human serum.