This study applies molecular and morphological procedures to identify larval and adult stages of trematodes of the family Strigeidae in Iceland. Intermediate hosts (snails and fishes) and definitive host (birds) from 11 freshwater lakes were sampled and examined for the presence of trematodes. Recovered species were subjected to study of morphology, sequencing and phylogenetic analyses. A total of seven species of three genera were identified via phylogenetical analyses based on mitochondrial (cox1) and nuclear (28S) sequences, and morphological data. The life-cycle of Apatemon gracilis was fully elucidated in Iceland, and those of Australapatemon burti and Australapatemon minor in part (cercariae and adults). The relationship of Cotylurus sp. 'Lin. 1I' and Cotylurus sp. 'Lin. 2I' could not be resolved.