No description available.

Globally, karst ecosystems are experiencing anthropogenic impacts due to their high hydrologic connectivity, leading to issues including increasing color (browning) as noted in recent decades at the largest freshwater spring on Earth (Wakulla Springs, Florida, USA). Wakulla Springs is located within a karst landscape, characterized by numerous sinkholes connecting surface and groundwaters. Surface waters and associated dissolved organic matter (DOM) from both natural sources (e.g. Apalachicola National Forest) and anthropogenic sources (e.g. municipal wastewater facility, sprayfield farm and diffuse inputs from the city of Tallahassee) are transported through these features and discharged at Wakulla Springs’ vent. In this study we assess dissolved organic matter (DOM) composition via absorbance and fluorescence spectroscopy and Fourier Transform ion cyclotron mass spectrometry (FT-ICR MS) to evaluate seasonal changes in DOM composition and dissolved organic carbon (DOC) concentration. Samples were collected between August 2015 and August 2016, bi-weekly, at three wells and two fluvial sites throughout Wakulla Springs State Park. These wells represent conduits feeding from different locations in the springshed, encompassing the major inputs and sources of DOM to the Spring vent and downstream Wakulla River. Sample sites separated into distinct groups based on DOC concentration and optical and FT-ICR MS parameters indicative of autochthonous (clear groundwater) versus allochthonous (terrestrial) DOM. Seasonal trends in DOM composition at Wakulla Springs vent are apparent and predominantly driven by high precipitation events and associated inputs of DOM from the Apalachicola National Forest with increased color, DOC, and terrestrially sourced molecular signatures. Principle component analysis highlights the ability of optical parameters to show the dominance of sample sites draining from the southwest (i.e. Apalachicola National Forest) as responsible for the color rich water at the vent, whereas water draining from the north was comparatively clear. Multivariate analyses demonstrates how FT-ICR MS can clearly distinguish the molecular signatures of clear groundwaters and afforested blackwater inputs, and emphasize the dominance of conduits draining from afforested sites as controlling the DOM signature and associated color at the vent. Despite the hydrologic complexity of the aquifer, FT-ICR MS allowed for clear discrimination between the different DOM sources and so these methodologies may be applied to aquifers around the world to trace inputs and evolved signatures. Increasing colored rich waters at the vent suggests that either input from conduits draining from the southwest have increased, or the relative dilution with clear groundwater has decreased in the conduit system prior to discharge from the vent. Sea-level rise impacts in the region have been suggested to result in more blackwaters delivered to the vent, and ongoing extraction of clear groundwater reduces the dilution capacity on color rich waters. Thus, anthropogenic impacts in the region need to be addressed if the trend of increased colored inputs at Wakulla Springs is to be reversed. / A Thesis submitted to the Department of Earth, Ocean and Atmospheric Science in partial fulfillment of the requirements for the degree of Master of Science. / Spring Semester 2018. / March 28, 2018. / Absorbance, Biogeochemistry, Dissolved Organic Matter, Fluorescence, Fourier Transform ion cyclotron resonance mass spectrometry, Freshwater Springs / Includes bibliographical references. / Robert Spencer, Professor Directing Thesis; Jeffrey Chanton, Committee Member; Mariana M. P. B. Fuentes, Committee Member.

The thesis took an interdisciplinary, problem-oriented approach to address the research problem: ’Is farmed fish capable of replacing wild-caught fish in the Malaysian diet?’ The main objectives of the project were to assess current fish consumption habits, evaluate the impacts of these on sustainability of fish stocks and determine whether aquacultured products could be a suitable substitution. A combination of data collection methods was used, namely dietary assessments, systematic literature review, market research interviews and surveys and analysis of electronic and paper-based official records. The review of the Malaysian food balance sheets showed a significant transition in diet over the last three decades, specifically with respect to an increase in animal protein by approximately 60% over this time, with fish as the major source of protein. To further explore the contribution of fish to the diet of Malaysians, and any ethnic and geographical differences in consumption, a food frequency questionnaire (FFQ) was developed to assess habitual dietary intakes within selected coastal, rural and urban populations across different cultural groups in Klang Valley. The FFQ provided a clearer picture of the quantities of fish consumed by Malaysians and enabled the characterisation of Malaysian fish consumers based on the types of fish species consumed for sustainability assessment purpose. The nutritional contribution of wild versus farmed fish was compared and contrasted. Results from this study, combined with detailed studies of perception of wild versus farmed fish by consumers, aquaculturists and wholesalers, provided a greater understanding of the factors that influence consumers’ fish buying and consumption habits. The key findings are discussed with respect to the sustainability of the current situation, potential for expanding the aquaculture sector to replace wild fish in the Malaysian diet and recommendations for future research and issues for policy makers involved in the expansion of the industry.

Recent studies have demonstrated that the morphologically similar white marlin (Kajikia albida) and roundscale spearfish (Tetrapturus georgii) co-occur in the western North Atlantic, including the U.S. Mid‐Atlantic Bight. Differences in scale morphology have been proposed as one morphological character to discriminate these species, but a thorough analysis of scale morphology is lacking. Because the validity of the roundscale spearfish was not established until 2006, much of the biological information previously collected for “white marlin” may include data for both white marlin and roundscale spearfish. The objectives of this study were to obtain a better understanding of the movements and habitat utilization of positively identified white marlin that inhabit the U.S. Mid-­‐Atlantic Bight during summer months, and to describe the morphological variation of white marlin and roundscale spearfish scales. Eleven long‐term (6 or 12 month) pop-up satellite archival tags were placed on white marlin that were caught and released in the U.S. recreational fishery. Nine tags reported information on temperature, pressure (depth), and light levels for light‐based geolocation for periods of 8 days to 12 months. Most fish moved out of the Mid-Atlantic Bight in September, and overwintered in areas ranging from east of the Gulf Stream off the Carolinas to the Caribbean, and as far south as northern Brazil. of the seven fish that retained tags for more than 40 days, five spent time in known spawning grounds in waters of the Dominican Republic leading up to the spring spawning season. These data demonstrate a large degree of connectivity among white marlin in the western North Atlantic. as noted in previous studies, individuals spent a large proportion of their time in the surface waters (0-10m; 75% across all white marlin pooled), the vast majority of their time in the top 100 m of the water column (97%), and within eight degrees of sea surface temperature (98%), although definite shifts in habitat utilization were evident as fish departed coastal offshore waters of the Mid‐Atlantic Bight. Diel habitat utilization varied greatly, with white marlin spending 81% of total nighttime in the surface waters (0‐10m), and only 26% of total daytime in surface waters. Past studies have characterized the scales of white marlin and roundscale spearfish as being morphologically distinct, but little effort has been made to describe variation within an individual, among individuals, or between species. to better understand morphological variation of scales and squamation patterns of distinct body regions of these two species, individual scales were collected from 11 specific anatomical regions, and scale patches were collected from 3 specific regions of each white marlin and roundscale spearfish brought into marlin tournament weigh stations in the Mid-Atlantic Bight during 2012 and 2013. Scales were measured and described, and scale patches were cleared and stained to examine the level of imbrication of the scales, as well as the overall squamation patterns. In addition to the scales, denticular plates, ossified formations occurring on the surface layer of the dermis, were measured and described. Although considable morphological variation was observed among scales from different anatomical regions of individuals of both species, white marlin scales generally have pointed anterior ends, fewer posterior points, and are more heavily imbricated than those of roundscale spearfish, which are frequently rounded anteriorly, but often have many posterior points and are farther separated within the skin. Over all areas and individuals, roundscale spearfish scales were significantly wider and had a lower length-­‐to-­‐width aspect ratio than those of white marlin. Detailed scale descriptions allow for a more accurate characterization of the variation within and differences between these two species, and could potentially be a valuable tool for investigating istiophorid systematics.

No description available.

Thesis (doctoral)--Wageningen University, 2006. / Vita. "Propositions" ([1] leaf) inserted. Includes bibliographical references.

Epilithic biofilms were monitored for potential impacts of an experimental rainbow trout aquaculture operation at the Experimental Lakes Area in northwestern Ontario, Canada. Metabolic and particulate samples were collected from the middle littoral zone of the experimental and reference lakes before and during the aquaculture operation. Phosphorus stocks in the experimental lake (epilimnetic and epilithic) increased as a result of both food waste and fish excretion. These cumulative P inputs were predicted to increase epilithic biomass and productivity, cause major compositional changes in the dominant groups and increase epilithic algal toxin production (microcystins). However, no major aquaculture-related changes in algal biomass, productivity, toxins or group dominance were observed in the epilithon. The only observed changes occurred at the species-level. These species-level changes were transient but did allude to an ecosystem reacting to stress. / February 2009

Thesis (M. Agr. Sc.)--University of Queensland, 2002. / Includes bibliographical references.

Thesis (Ph. D.)--University of Queensland, 2002. / Includes bibliographical references.

Riverine fisheries in England are under pressure from a variety of activities, including increasing intensification of land-use, urbanisation, rising demands for water abstraction, pollution, proliferation of exotic species, climate change and recreational activities. As a result, the integrity of English rivers has changed. In this study, an attempt was made to measure the ecological health of 22 English lowland rivers from the Thames, Trent and Yorkshire Ouse catchments using a variety of tools. The objective was to modify the Index of Biotic Integrity (IBI) for use on English lowland rivers and compare it with existing indices. A number of diversity indices, Margalef (DMg), Simpson (Dsm) and ShannonWiener (H') were used to evaluate the status of fisheries in the study rivers. The Abundance / Biomass (ABC) method and computer-based multivariate analyses, UPGMA, TWINS PAN, DECORANA, were also used to evaluate the status of fish communities. In addition to these indices, the ABC method and multivariate analyses, the IBI, a multimetric index was also used to evaluate the ecological health of study rivers. The IBI is based on structural and functional attributes of fish communities and is capable of evaluating health and condition of an aquatic ecosystem. The IBI requires a reference condition with which to compare the output. In English rivers, no pristine (reference) sites were considered available, consequently best available data were used to develop a reference condition. In this study, the IBI was modified from Karr (1981), which was based on 12 metrics (community characteristics) of fish assemblages. For the study rivers, 15 metrics which described the status of the fish communities were selected to calculate the IBI. Each metric was scored on a simple scale from 0 (absence) to 5 (high quality). The sum of all the metrics (range 0 - 75) was used to assign sites to qualitative classes of biotic integrity. Six integrity classes on a continuous scale were chosen with the following class boundaries: Excellent (56 - 75), Good (42 - 55), Fair (28 - 41), Poor (16 - 27), Very Poor (1 - 15) and No Fish (0). In the study rivers, the DMg, Dsm and H' indices were unable to measure anthropogenic impacts on fish communities as all these indices were based on structural properties of fish communities. These indices also failed to take account of the presence of juveniles in the fish community in a river. Moreover, these indices were influenced by dominant species abundance and sampling strategies, giving an inaccurate assessment of the status of the fisheries. The ABC method was better at evaluating fish communities than diversity indices as the method considered fish abundance and biomass. However, the method did not include functional components of the fish community and was over influenced by juvenile fishes. Consequently, the ABC method was not considered a good indicator of ecosystem health based on fish assemblages. The UPGMA, TWINSPAN and DECORANA analyses, successfully grouped and separated river reaches with rich or poor fish stocks. These analyses however, did not take into account the functional attributes of the fish communities and were not sufficient to explain the status of a fishery without support from other indices. The IBI assessed the ecological health of the middle and lower reaches of the study rivers more accurately than the other diversity indices, ABC method and multivariate analyses. The selected IBI metrics were able to evaluate many perturbations and disturbances as the metrics represented both structural and functional attributes of fish communities. The DMg, Dsm, H', ABC, UPGMA, TWINSPAN and DECORANA were designed to highlight a specific attribute and lost information during calculation but the IBI included a greater variety of information and produced an appropriate index. Spearman's rank correlation indicated the IBI outputs were more similar to diversity indices than other measures, as significant relationships were found between the IBI and DMg, the IBI and Dsm, and the IBI and H' at a = 0.01 level. Significant relationships were probably due to the use of fish density and abundance in the models. However, this did not mean that all diversity indices and the IBI were similar in measuring ecological conditions of a river, rather it was probably numerical similarity. No significant relationship was found between the IBI and ABC, as the ABC index was a ratio of abundance and biomass while the IBI used absolute values of biomass and abundance separately. All the diversity indices, ABC method and multivariate analyses mentioned reinforced the view that the IBI developed in this study was an appropriate index at evaluating ecological health of the middle and lower reaches of the study rivers. The IBI, however, failed to predict the quality of the fisheries in headwater streams because of the exclusion of salmonid species, minor species and general low species diversity found in these zones. Consequently, it was identified that reference conditions and metrics chosen for the middle and lower reaches of the study rivers were not appropriate to assess the ecological health of headwaters. The existing monitoring programmes of the Environment Agency (EA) for fishery data collection, were considered appropriate for calculating IBIs. Sampling strategies of the EA, i.e. daytime, electric fishing both in summer and winter periods irrespective of lunar cycle and breeding season were also considered acceptable to calculate the IBI. Further research was recommended to test the IBI on a wide range of rivers to assess whether the IBI is appropriate for assessing ecological health of middle and lower reaches of rivers in all regions of the UK. Separate IBIs for headwaters, still waters and estuaries were proposed as these zones / waterbodies have different fish communities. Investigation should be directed at developing a simplified IBI using other cost-effective data sources if suitable resources are not available. It is also recommended that the possibility of including the IBI in wider aquatic resource monitoring programmes (e.g. WFD) be investigated. It is also recommended that the possibility of using the IBI to detect change in the pre and post implementation periods of any management action or anthropogenic activity be investigated. Research is also needed to integrate the IBI with other bioassessment methods (e.g. Habitat index, Diatom index, Microinvertebrate index, Chemical index and GQA index). For more effective application and understanding, the IBI may be built into a GIS (Geographical Information System) environment. It is suggested that a suitable computer package be developed to simplify calculations of the IBI. The interpretation should however, be carried out by the fishery manager or scientist.