The process of ubiquitination plays an essential role in numerous cell functions, including apoptosis and the induction of immune responses. Ariadne 2 is a RING finger E3 ligase and is part of the highly conserved RBR (RING-B-Box-RING) superfamily, however, little is known of its function in mammalian systems. To further examine the physiological role, Ariadne 2 deficient mice were generated. In a mixed background, Ariadne 2 deficient (Arih2-/-) mice die prematurely after birth however lethality is not fully penetrant. Adult mice that escape lethality have lower body weight and reduced viability due to an apparent lymphoproliferative disorder. In a C57BL/6 background, Ariadne 2 deficiency leads to a fully penetrate embryonic lethality, occurring after embryonic day 16.5. Arih2-/- foetal liver have reduced cellularity and increased apoptosis, however haematopoietic cells are capable of differentiating into myeloid and granulocytic progenitors and can fully reconstitute lethally irradiated Rag1-/- recipient mice. These Rag1-/-Arih2-/- chimeras recapitulate the lymphoproliferative disorder observed in the mixed background Arih2-/- mice. Further analysis show Rag1-/-Arih2-/- chimeras display increased number of lymphocytes, granulocytes, macrophages and dendritic cells, increased serum immunoglobulin levels and pro-inflammatory cytokines, and dramatic heterogeneous cellular organ infiltration, consisting mainly of T cells. T cell homeostasis is also altered, as seen by increased activated and ‘memory-like’ T cells, elevated TH1 and TH2 cytokines, increased regulatory T cells (Treg), and increased T cell proliferation. This may be due to an observed premature maturation of Arih2-/- dendritic cells. Arih2-/- foetal liver derived dendritic cells (FLDC) express high levels of maturation markers CD80/B7.1, CD86/B7.2, CD83, CD40 and MHCII and are capable of activating T cells in the RIP-GP model of induced diabetes. This may be linked to modulation of the NFκB and ERK pathways, in particular increase in nuclear p65/RelA and phospho-p65/RelA leading to an increase in NFκB and AP-1 binding to DNA and sustained and hyperactive NFκB response in Arih2-/- dendritic cells. Overall, Ariadne 2 is shown to be a negative regulator in the activation of immune cells, in particular dendritic cells, and is a novel regulator in the maintenance of peripheral tolerance and the pathogenesis of autoimmunity.

The process of ubiquitination plays an essential role in numerous cell functions, including apoptosis and the induction of immune responses. Ariadne 2 is a RING finger E3 ligase and is part of the highly conserved RBR (RING-B-Box-RING) superfamily, however, little is known of its function in mammalian systems. To further examine the physiological role, Ariadne 2 deficient mice were generated. In a mixed background, Ariadne 2 deficient (Arih2-/-) mice die prematurely after birth however lethality is not fully penetrant. Adult mice that escape lethality have lower body weight and reduced viability due to an apparent lymphoproliferative disorder. In a C57BL/6 background, Ariadne 2 deficiency leads to a fully penetrate embryonic lethality, occurring after embryonic day 16.5. Arih2-/- foetal liver have reduced cellularity and increased apoptosis, however haematopoietic cells are capable of differentiating into myeloid and granulocytic progenitors and can fully reconstitute lethally irradiated Rag1-/- recipient mice. These Rag1-/-Arih2-/- chimeras recapitulate the lymphoproliferative disorder observed in the mixed background Arih2-/- mice. Further analysis show Rag1-/-Arih2-/- chimeras display increased number of lymphocytes, granulocytes, macrophages and dendritic cells, increased serum immunoglobulin levels and pro-inflammatory cytokines, and dramatic heterogeneous cellular organ infiltration, consisting mainly of T cells. T cell homeostasis is also altered, as seen by increased activated and ‘memory-like’ T cells, elevated TH1 and TH2 cytokines, increased regulatory T cells (Treg), and increased T cell proliferation. This may be due to an observed premature maturation of Arih2-/- dendritic cells. Arih2-/- foetal liver derived dendritic cells (FLDC) express high levels of maturation markers CD80/B7.1, CD86/B7.2, CD83, CD40 and MHCII and are capable of activating T cells in the RIP-GP model of induced diabetes. This may be linked to modulation of the NFκB and ERK pathways, in particular increase in nuclear p65/RelA and phospho-p65/RelA leading to an increase in NFκB and AP-1 binding to DNA and sustained and hyperactive NFκB response in Arih2-/- dendritic cells. Overall, Ariadne 2 is shown to be a negative regulator in the activation of immune cells, in particular dendritic cells, and is a novel regulator in the maintenance of peripheral tolerance and the pathogenesis of autoimmunity.

Thesis (MA)--University of Melbourne, School of Historical Studies, 2010. / Typescript. Includes bibliographical references (p. 83-91)

This thesis takes Ariadne's abandonment as a case study in order to examine the literary processes of reception that underlie the transmission of classical myth in different eras and cultural contexts - from Classical Antiquity through the Italian Renaissance. Rather than focusing on the ways in which visual representations of Ariadne relate to literary treatments, it draws attention to the literary reliance on a cultural framework, shared by writer and reader, that enables dynamic storytelling. It argues that literary variation of the myth is central to its successful transmission, not least because it allows for appropriations and adaptations that can be made to fit new social and religious parameters, such as Christian conventions in the Middle Ages. In focusing on the important role played by the visual arts in the classical tradition, this research further challenges the still prevalent misconception that the visual arts are secondary to literature, and refutes the common assumption that the relationship between image and text is unidirectional. It highlights the visual impulses leading to paradigm shifts in the literary treatment of the abandonment narrative, and examines the ways in which writers engage with the visual tradition in order to re-shape the ancient narrative. Throughout, attention is drawn to the visual and cultural framework shared by ancient writers and readers, and to the lack of engagement with this framework in traditional classical scholarship. Through its focus on the literary narratives' visuality and mutability, this thesis offers a new paradigm for studying classical myth and its reception.

The thesis consists of detailed commentary on the first 201 lines of Catullus 64, together with an edited text and apparatus criticus. It represents about half a planned commentary on the whole poem, which will also include an introduction. The commentary begins by discussing the poem’s Argonautic opening, its use of allusion to negotiate generic relationships with epic and tragedy, and its exploration of narrative, pictorial and first-person ‘lyric’ modes. As the narrative jumps to Peleus’ wedding, the commentary examines the complicated moral signals about Roman luxury and the golden age sent by the description of the gleaming palace surrounded by abandoned fields. The transition to the description of Ariadne prompts an examination of how this ‘disobedient’ ecphrasis emphasises details that a picture could not literally convey, together with an analysis of the male narrator’s objectifying presentation of a woman in distress. The ecphrasis proper is then disrupted by a ‘flashback’ covering Ariadne’s first encounter with Theseus and his fight with the Minotaur: the commentary explores the ways in which shifting focalisation complicates the reader’s judgement of Theseus’ heroism. Finally, the thesis looks at Ariadne’s speech as an intertextual node, investigating the meanings generated by its relationships with other speeches from both earlier and later in Greek and Roman poetic traditions, and examining how each theme or topos is used in this particular situation both by the alluding poet and by Ariadne herself. More discursive notes introducing the various sections are interspersed with shorter lemmata considering textual, metrical, linguistic and cultural-historical issues as well as literary interpretation. The commentary aims both to open up the possibilities of meaning offered by individual words and phrases, and to advance critical understanding of key aspects of the whole poem, such as its narratorial voice, engagement with visuality and place in literary history.

The performance of my opera/art installation, Ariadne, on April 2 and 4, 1993, in Recital Hall, culminated a year of research, composition, production, and rehearsal upon the Ariadne Project, an interdisciplinary art collaboration. My project brought together the research, creative, and performance skills of many individuals throughout the university community and beyond. Designed as a companion paper to this composition and performance, this paper documents the inception, creation, production, and performance of the Ariadne Project. It also addresses the issues and sources dealt with and assessess the effectiveness of the product and the process. / Department of Art

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Thesis (M.V.A.)--University of Sydney, 2009. / Title from title screen (viewed November 26, 2009) Submitted in fulfilment of the requirements for the degree of Master of Visual Arts to the Sydney College of the Arts, University of Sydney. Degree awarded 2009; thesis submitted 2008. Includes bibliographical references.

This thesis uncovers and historicizes an overlooked aspect of America’s transgender history. The heterosexual male cross-dressers, or transvestites, of mid-century America constituted a group of individuals that espoused a particular discourse of respectability in their cross-gender practices, conceptualized unique bi-gender identities, and cultivated a community. Heterosexual male cross-dressers, under the leadership of Virginia Prince and Ariadne Kane worked to separate themselves from broader, and more recognizable, identities such as gay transvestites, drag queens, and homosexuals in an effort to define themselves as respectable. A critical historical analysis of Fantasia Fair indicates that Prince and Kane were not alone in their desire for a community of their peers, with whom to share ideas about sexological theories, personal stories, and tactics for self-preservation. As a direct response to the pervasive nature of transsexual narratives in the field of transgender history, this project demonstrates the important advances made by heterosexual male cross-dressers to our modern understanding of trans diversity. These cross-dressing narratives prompt historians of transgender phenomena to think critically about the diversity of identity categories that are encompassed in our present understanding of the term ‘transgender’. / Graduate / 2019-07-30