Thesis (M.S.)--Worcester Polytechnic Institute. / Keywords: secondary metabolites; hairy roots; tetraploid; artemisinin. Includes bibliographical references (p. 83-106).

In most plant species artificial polyploidy generally enhances the vigor of determinate plant parts and may be favorable where vegetative organs and biomass constitute the economic product. Furthermore, artificial polyploidy has been considered a method of increasing production potential of plants secondary metabolites. However, despite considerable research on polyploid plants, very few cases of polyploid medicinal plants have been reported. Artemisia annua L. synthesizes artemisinin, an antimalarial sesquiterpene lactone. Artemisinin can be synthesized, but it is costly compared to the naturally derived product. Hairy root cultures of Artemisia annua L. (clone YUT16) show rapid growth and produce artemisinin. This culture offers a good model system for studying artemisinin production. Others have shown that tetraploid Artemisia annua L. plants produce more artemisinin/mg DW than diploids. These yields were offset, however, by decreases in biomass productivity. Little is known about how polyploidy may affect growth production of hairy roots. Using colchicine, we have produced four stable tetraploid clones of Artemisia annua L. from YUT16 hairy root clone. Compared to the diploid clone, these tetraploid clones showed major differences in growth and development. Nevertheless, artemisinin yields of these tetraploid clones were 2-5 times higher than the diploid and their production seemed to be by the age of the inoculum. This work will prove useful in furthering our understanding of the effects of artificial polyploidy on the growth and secondary metabolite production of hairy roots.

Artemisia Gentileschi and Caravaggio's Looking Glass is an ironic allusion to both the concave mirror and the biconvex lens. It was these simple objects, in colloquial terms a shaving mirror and a magnifying glass, which Artemisia Gentileschi and her father Orazio, learned from Caravaggio how to use to enhance the natural phenomenon of the camera obscura effect. Painting from a projection meant that Artemisia could achieve an extreme form of realism and detail in her work. This knowledge, which was of necessity kept hidden, spooked the Inquisition and also gave artists, who knew how to manipulate the technology, an extreme competitive edge over their rivals. This dissertation challenges the naive assumptions that have been made about Artemisia's working practices, effectively ignoring the strong causal links between art and science in Seicento Italian painting. Introducing the use of optical aids by Artemisia opens up her story to a whole new generation of scholarship. / Art History / M.A. (Art history)

Artemisia Gentileschi and Caravaggio's Looking Glass is an ironic allusion to both the concave mirror and the biconvex lens. It was these simple objects, in colloquial terms a shaving mirror and a magnifying glass, which Artemisia Gentileschi and her father Orazio, learned from Caravaggio how to use to enhance the natural phenomenon of the camera obscura effect. Painting from a projection meant that Artemisia could achieve an extreme form of realism and detail in her work. This knowledge, which was of necessity kept hidden, spooked the Inquisition and also gave artists, who knew how to manipulate the technology, an extreme competitive edge over their rivals. This dissertation challenges the naive assumptions that have been made about Artemisia's working practices, effectively ignoring the strong causal links between art and science in Seicento Italian painting. Introducing the use of optical aids by Artemisia opens up her story to a whole new generation of scholarship. / Art History / M.A. (Art history)