Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical analysis Candidate Michaela Beranová Supervisor doc. PharmDr. Miroslav Miletín, Ph.D. Title of thesis Preparation of fluorescent azaphthalocyanines for labeling of oligonucleotide probes I. Azaphthalocyanines (AzaPc) are macrocyclic compounds with large system of conjugated bonds and are therefore used as dyes and pigments. Their potential as diagnostic and therapeutic means is also under intensive examination. The aim of this thesis is a synthesis of precursors for cyclotetramerization of asymmetric fluorescent tetrapyrazinoporphyrazines (TPyzPz) via solid phase method bearing two various moieties, one suitable for binding to solid phase and the other intended for binding of the final TPyzPz to oligonucleotides. At the beginning of the thesis, basic terms related to AzaPc are defined. Also the main principles of synthesis of TPyzPz as well as possible modifications of their final structure are discussed. An important part of the thesis is the description of the typical properties of TPyzPz, the influence of the structure on the individual parameters and the fields of their use. Afterwards the preparation of each precursor with two different substituents for cyclotetramerization is...