Global ETD Search

1191	Studies on the Effects of Carbon Nanomaterials and Efflux Pump Inhibitors on Biofilm Formation and Lipid Biosynthesis in Mycobacterium smegmatis Rashmika Gunda (17555157) 07 December 2023 (has links) <p dir="ltr">Tuberculosis remains a global health challenge, ranking as the second leading cause of mortality worldwide in 2022. The resilience of <i>Mycobacterium tuberculosis</i>, the causative agent of tuberculosis, is enhanced by the high expression of efflux pumps that confer antibiotic tolerance and the formation of biofilms that confer resistance to antibiotics. Carbon nanomaterials (CNMs) exhibit a broad-spectrum of antibacterial efficacy, making them promising candidates for combating drug-resistant bacterial strains. The effects of the novel carbon allotropes called fullertubes (C<sub>90</sub>) on any living cell have not been studied. In our study, we employed <i>Mycobacterium smegmatis</i> as a model organism for <i>M. tuberculosis</i> and exposed it to fullertubes and fullerenes. We explored the impact of these CNMs on efflux activity and biofilm formation through biochemical assays like ethidium bromide transport assay and crystal violet assay. We also investigated their impact on lipid biosynthesis associated with log-phase growth and biofilm formation using metabolic radiolabeling studies. We also investigated the effects of the efflux pump inhibitors (EPIs) piperine, berberine, 1-(1-naphthylmethyl)-piperazine and thioridazine on efflux activity, biofilm formation, and lipid biosynthesis associated with log-phase growth and biofilm formation in <i>M. smegmatis.</i> We utilized metabolic radiolabeling methods using <sup>14</sup>C-palmitic acid and <sup>14</sup>C-acetic acid which are precursors of lipid biosynthesis and analyzed the lipids by silica-thin layer chromatography and autoradiography. Our studies revealed that CNMs do not influence efflux activity. However, efflux pump inhibitors effectively block efflux activity in <i>M. smegmatis</i>. Biofilm formation was decreased by CNMs and EPIs. In biofilm cells, fullertubes increased the incorporation of radiolabeled <sup>14</sup>C-palmitic acid into glycopeptidolipids on the cell surface as well as inside the cell. Piperine and berberine affected the incorporation of the radiolabels into lipids such as trehalose monomycolate, phosphatidylethanolamine and cardiolipin in planktonic and biofilm cells. Our study provides insights into the diverse effects of CNMs and efflux pump inhibitors on <i>M. smegmatis</i>.</p> Analytical biochemistry Infectious agents Fullerenes C 60 Fullertubes C 90 Efflux pump inhibitor Mycobacterium smegmatis cell envelope Biofilm formation
1192	The O-Antigen Capsule of Salmonella Typhimurium in Acute and Chronic Infection Marshall, Joanna M. January 2013 (has links) No description available. Biomedical Research Microbiology Molecular Biology Microbial Pathogenesis Salmonella Typhi Salmonella Typhimurium O antigen capsule Biofilm Lipopolysaccharide Flagella
1193	In situ measurement of mercury ecotoxicological effects on stream periphyton in southwest Ohio Klaus, Jaclyn Elizabeth 05 September 2014 (has links) No description available. Biology Aquatic Sciences Chemistry Environmental Science Periphyton MMHg monomethylmercury ecotoxicology primary producer metabolic activity biomass toxicity sediment biofilm in situ
1194	Mechanism of Biocorrosion Caused by Biofilms and Its Mitigation Liu, Jialin January 2017 (has links) No description available. Biomedical Engineering Microbiology Biochemistry 2205-Cu duplex stainless steel Pseudomonas aeruginosa Desulfovibrio vulgaris Microbiologically influenced corrosion Biofilm Antimicrobial mechanism
1195	Effect of Yarrowia Lipolytica biofilm on corrosion behavior of carbon steel in simulated biodiesel storage tanks Nabati, Zahra January 2017 (has links) No description available. Chemical Engineering Engineering
1196	An Investigation into the Impact of Cell Metabolic Activity on Biofilm Formation and Flux Decline during Cross-flow Filtration of Cellulose Acetate Ultrafiltration Membranes Mohaghegh Motlagh, Seyed Amir H. January 2011 (has links) No description available. Civil Engineering Environmental Engineering ultrafiltration membrane crossflow filtration biofouling membrane biofilm cell metabolic activity adenosine triphosphate ATP CTC
1197	Investigation of Nutrient Limitation of the Biofilm Community in Acid Mine Drainage Impaired and Remediated Streams Keil, Emily J. January 2016 (has links) No description available. Ecology Freshwater Ecology nutrient diffusing substrates acid mine drainage biofilm fatty acids extracellular enzyme activity remediation nutrient limitation
1198	Identification of microorganisms in food ecosystems and characterization of physical and molecular events involved in biofilm development Luo, Hongliang 02 December 2005 (has links) No description available. Real-time PCR detection Alicyclobacillus juice Listeria monocytogenes CluA conjugation biofilm Lactococcus lactis antibiotic resistance food ecosystem
1199	Noninvasive immunization strategies to target dendritic cells and protect against experimental otitis media due to nontypeable <i>Haemophilus influenzae</i> Novotny, Laura Anne 21 March 2011 (has links) No description available. Immunology Microbiology otitis media nontypeable Haemophilus influenzae vaccine biofilm chinchilla dendritic cell LB1 OMP P5 PilA Type IV pilus
1200	Characterization of Ultrafiltration Membranes and Effect of Biofouling on Their Water Treatment Performance Zaky, Amr M. 09 June 2011 (has links) No description available. Civil Engineering Environmental Engineering ultrafiltration membrane biofouling chemical force microscopy atomic force microscopy crossflow filtration morphology analysis characterization techniques biofilm

Search results