Dissipative spots are found in physical experiments of many branches of natural science. In this thesis we use three-component reaction-diffusion systems on two-dimensional domains in order to generate these patterns. Using a dynamical system approach we proceed with a Fourier analysis on a linearized reaction-diffusion system in order to provide the bifurcation conditions for a given homogeneous state. We validate our results and establish it's limitations through numerical experiments. We report very interesting behavior during these simulations, notably hysteresis and multi-stability. We will then turn our attention to the relatively unexplored phenomenon of rotating spots. Based on previous work done for spiral waves, we investigate the effect of translational symmetry-breaking on a rotating spot mainly through careful numerical analysis.

<p>Polarization is important for the function and morphology of many different cell types. The keys regulators of polarity in eukaryotes are the Rho-family GTPases. In the budding yeast Saccharomyces cerevisiae, which must polarize in order to bud and to mate, the master regulator is the highly conserved Rho GTPase, Cdc42. During polarity establishment, active Cdc42 accumulates at a site on the plasma membrane characterizing the “front” of the cell where the bud will emerge. The orientation of polarization is guided by upstream cues that dictate the site of Cdc42 clustering. However, in the absence of upstream cues, yeast can still polarize in a random direction during symmetry breaking. Symmetry breaking suggests cells possess an autocatalytic polarization mechanism that can amplify stochastic fluctuations of polarity proteins through a positive feedback mechanism.</p><p> Two different positive feedback mechanisms have been proposed to polarize Cdc42 in budding yeast. One model posits that Cdc42 activation must be localized to a site at the plasma membrane. Another model posits that Cdc42 delivery must be localized to a particular site at the plasma membrane. Although both mechanisms could work in parallel to polarize Cdc42, it is unclear which mechanism is critical to polarity establishment. We directly tested the predictions of the two positive feedback models using genetics and live microscopy. We found that localized Cdc42 activation is necessary for polarity establishment.</p><p> While this explains how active Cdc42 localizes to a particular site at the plasma membrane, it does not address how Cdc42 concentrates at that site. Several different mechanisms have been proposed to concentrate Cdc42. The GDI can extract Cdc42 from membranes and selective mobilize GDP-Cdc42 in the cytoplasm. It was proposed that selectively mobilizing GDP-Cdc42 in combination with local activation could locally concentrate total Cdc42 at the polarity site. Although the GDI is important for rapid Cdc42 accumulation at the polarity site, it is not essential to Cdc42 concentration. It was proposed that delivery of Cdc42 by actin-mediated vesicle can act as a backup pathway to concentrate Cdc42. However, we found no evidence for an actin-dependent concentrating pathway. Live microscopy experiments reveal that prenylated proteins are not restricted to membranes, and can enter the cytoplasm. We found that the GDI-independent concentrating pathway still requires Cdc42 to exchange between the plasma membrane and the cytoplasm, which is supported by computational modeling. In the absence of the GDI, we found that Cdc42 GAP became essential for polarization. We propose that the GAP limits GTP-Cdc42 leak into the cytoplasm, which would be prohibitive to Cdc42 polarization.</p> / Dissertation

Title: Dynamical symmetry breaking in models with strong Yukawa in- teractions Author: Petr Beneš Department: Institute of Theoretical Physics, Faculty of Mathematics and Physics, Charles University Supervisor: Ing. Jiří Hošek, DrSc., Department of Theoretical Physics, Nu- clear Physics Institute, Academy of Sciences of the Czech Repub- lic Abstract: The primary aim of the thesis is to explore the possibility of spon- taneous symmetry breaking by strong Yukawa dynamics. Tech- nically, the symmetry is assumed to be broken by formation of symmetry-breaking parts of both the scalar and the fermion prop- agators, rather than by the scalar vacuum expectation values. The idea is first introduced on an example of a toy model with the underlying symmetry being an Abelian one and later applied to a realistic model of electroweak interaction. In addition, the thesis also deals with some more general, model-independent is- sues, applicable not only to the discussed model of strong Yukawa dynamics, but to a wider class of models with dynamical mass generation. First of these issues is the problem of fermion flavor mixing in the presence of fermion self-energies with a general mo- mentum dependence. It is in particular shown how to define the CKM matrix in such models and argued that it can come out in principle...

Simulations of the circulation in the middle atmosphere during northern winter performed with a nonlinear, mechanistic, global circulation model show that the upper mesospheric jet is greatly overestimated and also the position with respect to latitude and height does not correspond to observations. Apart from that also the winter wind reversal in the mesopause region, evoked by breaking gravity waves (GWs), is located too low around 80km, but is observed to be usually around 100 km. These discrepancies are planned to be eliminated by modifying the distribution of GW amplitudes driving the GW parameterization. This distribution is currently based on potential GW energy data derived from GPS radio occultation measurements and has to be replaced by a distribution based on momentum flux estimates applying midfrequency approximation. The results show a weaker mesospheric jet more realistically tilted towards lower latitudes with height. Also the meridional circulation extending from the summer to the winter pole decelerates and less GWs are propagating into the mesosphere. By additionally varying the GW amplitudes in magnitude and time, the wind reversal is shifted upwards and the mesospheric jet is slowed down. / Simulationen der Zirkulation der mittleren Atmosphäre während des nordhemisphärischen Winters unter Verwendung eines nicht-linearen mechanistischen globalen Zirkulationsmodells ergaben beim Vergleich mit Messungen, dass der simulierte, mesosphärische Jet stark überschätzt wird und dessen Position von den Beobachtungen abweicht. Die in der Mesopausenregion einsetzende Windumkehr, hervorgerufen durch brechende Schwerewellen, befindet sich in etwa 80 km anstatt in 100 km. Diese Diskrepanzen sollen eliminiert werden. Hierfür wird die Verteilung der Schwerewellenamplituden, die die Schwerewellenparametrisierung innerhalb des Modells antreibt, am oberen Rand der Troposphäre modifiziert. Diese basiert derzeit auf global beobachteten, zonal gemittelten Daten der potentiellen Energie von Schwerewellen abgeleitet aus GPS Radiookkultationsmessungen und soll durch eine auf Impulsflüssen basierende Verteilung ersetzt werden. Das Modellexperiment zeigt, dass der mesosphärische Jet mit der Höhe in Richtung niedriger Breiten geneigt ist und abgebremst wird. Zudem schwächt die Meridionalzirkulation vom Sommer- zum Winterpol leicht ab und weniger Schwerewellen dringen bis in die Mesosphäre vor. Zusätzlich wird durch zeitliche und unterschiedlich starke Variation der Schwerewellenamplitude die Windumkehr verlagert und der mesosphärische Jet abgebremst.

The effect of maternal dietary fats on growth rate and bone development of commercial broilers was examined. Three hundred fifty female chicks were winged banded, weighed and equally divided among six starter pens (1.52 X 3.66m) with litter floors. At 20 wk of age, each pen was fed a basal laying diet supplemented with either 3% chicken fat (CF), soybean oil (SBO) or menhaden oil (MO). Each diet was provided with or without the antioxidant ethoxyquin, producing a total of six dietary treatments. Addition of fats [soybean (SBO), menhaden oil (MO), chicken fat (CF), soybean + antioxidant (SA), menhaden + antioxidant (MA), and chicken + antioxidant (CA)] to the maternal diet altered the tissue and yolk composition of hens to reflect the dietary source. Response variables measured were body weight, tibia weight and length, and breaking strength (stress, force, energy, bone wall, and diameter). Chick tissue from hens fed a MO and MA diet exhibited greater (P<0.01) amounts of DPA (22:5n3), DHA (22:6n3) and total n-3 fatty acids than the remaining dietary treatments. Tissues from chicks fed a SBO and SA diet displayed larger levels of 18:2n6 and total n-6 fatty acids when compared to all other treatments. Male and female chicks from the menhaden type diets (MO and MA) were lighter (P<0.01) during grow out period than from soybean (SBO and SA) and chicken (CF and CA) type diets. Chicks tibiae diameter from CF maternal diet tended to be larger than the MO maternal diet, with significance being noted at d 14 (P<0.01) and 28 (P<0.01). Increases were observed in shear force and stress required to break chick tibia from SBO maternal diet compared to those from the CF and MO maternal diets. The SBO maternal diet stimulates growth rate and bone development and strength of the progeny. (Key words: chickens, bone development, breaking strength, growth rate, fatty acids) / Master of Science

There are many ways to approach difficult subjects with children in preschool and the main focus in this thesis lays in the work with children and books. The purpose of the work has been to investigate the way teachers work with subjects that break the norm within books together with children in kindergarten. This study is based on four semi-structured interviews with teachers and Claes Nilholms concept of special needs education and Linda Pallas discourse analytic thinking. Furthermore, are the interviews based on eight questions that were open to interpretation and further conversations based on the participants' answers. The results show an awareness amongst the participants about the beneficial side of books as ways for integration. However, there seems to be a common conception that some subjects are only brought up in the group if there is a child that in some way represents the topic.

In the hospitality industry, the role of the frontline employee is integral. These employees are the face of the organization and have a strong role in shaping and forming the opinions of consumers by way of their product and service delivery. Therefore, the decisions an employee makes during the product or service delivery is critical in maintaining the relationship with the customer. Employees may be faced with opportunities to better service a customer at the cost of breaking an organizational rule or procedure. When an employee is faced with this dilemma and decides to break the rule on the behalf of the customer knowing the risks involved, this is called prosocial rule breaking. One distinct difference between this concept and general rule breaking is that this is performed as a nonselfish gesture; the employee does not receive any personal benefit. To examine this further, this study investigated the overall propensity to participate in prosocial rule breaking and the impact of the Big Five personality dimensions on prosocial rule breaking. To gain a better understanding of these constructs, a review of literature related to ethical decision making, prosocial behavior, and the five factor theory of personality was conducted. To investigate the research objectives, a purposive sample of frontline employees from a nationally branded restaurant chain completed a four part self-administered questionnaire by answering questions on the five factor personality dimensions through the Big Five Inventory (BFI), a restaurant based scenario followed by Morrison's (2006) prosocial rule breaking scale, a section on demographic information, and an open ended section for qualitative comments. Overall, three-hundred and five (305) usable questionnaires were completed and interpreted. The results demonstrated that this sample of restaurant employees revealed a moderate propensity for prosocial rule breaking. Moreover, the results revealed that the Agreeableness dimension is the most common personality dimension for this group of restaurant employees, but the Conscientiousness domain was the best predictor of one's propensity not to participate in prosocial rule breaking. The implications for managers from this study indicate a need for managers to recognize and encourage prosocial behaviors from their employees. They also need to understand which personality domains contribute to prosocial behavior, which can ultimately have implications for hiring, selection, and training.

Soybean mosaic virus (SMV; Genus Potyvirus; Family Potyviridae) is one of the most widespread viruses in soybean (Glycine max [L.] Merr.). Hutcheson, a cultivar developed in Virginia, is resistant to the common strains of SMV. However, new resistance-breaking (RB) isolates of SMV have emerged in natural infections to break the resistance of Hutcheson containing the Rsv1y allele. These RB isolates are SMV-G5 and G6-like based on the differential reactions on soybean cultivars with the Rsv1 locus, and are more G6-like based on the amino acid sequence of the coat protein (CP). The CP of the RB isolates is diverse at the amino and carboxy termini and highly conserved in the core region. RB isolates reduce the yield of susceptible cultivars and cause mottling of the seed coat. Dual infection of soybeans with SMV and BPMV increased the severity of symptoms, including plant stunting and SMV titer in comparison to single SMV inoculations. The reactions of Hutcheson and herbicide-tolerant Hutcheson RR were similar with or without herbicide application. Resistance to SMV is controlled by single dominant genes at three distinct loci, Rsv1, Rsv3 and Rsv4. The mechanisms of resistance at the Rsv3 and Rsv4 loci were investigated by tracking virus accumulation and movement over time using leaf immunoprints. The mechanisms of Rsv3 resistance include extreme resistance, hypersensitive response, or restriction to virus replication and movement, which are strain specific. The Rsv4 gene was found to function in a non-strain specific and non-necrotic manner. The mechanisms of Rsv4 resistance involve restricting both cell-to-cell and long distance movement of SMV. The Rsv1, Rsv3 and Rsv4 resistance genes exhibit a continuum of SMV-soybean interactions, and include complete susceptibility, local and systemic necrosis, restriction of virus movement (both cell-to-cell and long distance), reduction in virus accumulation, and extreme resistance with no detectable virus. Cultivars containing two genes for resistance, Rsv1 and Rsv3 or Rsv1 and Rsv4, were resistant to multiple strains of SMV tested and show great potential for gene pyramiding efforts to ensure a wider and more durable resistance to SMV in soybeans. / Ph. D.

Several truncated models for market weights are discussed. To summarize, the new truncated ordered stick breaking model introduced give restrictions on the ranks of the markets weights and show better fitting results for real data sets. / Thesis / Master of Science (MSc)

No description available.