This diploma deals with the changes and challenges that transportation companies will face at the beginning of 2010 due to the liberalization of the public transportation market. It is structured into a first part describing the theory and a second part that applies the theory into praxis. The theory focuses on the development of public transportation, the nomenclature, and specific terms related to the public transportation market, legal changes, financials and other influencing effects. The complete diploma is written with focus on bus transportation. The second part analyzes the current situation on the Czech public bus transportation market and further expected changes starting with 2010. General recommendations are made and possible strategies described how to establish in the market. Finally there is made an explicit recommendation for a specific bus company