The contemporary orthopaedics and traumatology of the musculoskeletal system and stomatology have been witnessing a substantial increase in the number of surgeries using metallic implants. The issue of reconstruction of bone defects covers a large area of study, where the surface properties of the implants are extremely important. Bone defects often occur as a result of open fractures, radical cancer treatment or limb lengthening, which is very common in paediatric orthopaedics. In the treatment of these conditions, the surface of the applied materials should provide a favorable environment for bone cells and support bone formation. In endoprosthetics it is highly desirable to achieve the strongest possible fixation between the implant surface and the bone. During the surgery, primary stability of the implant fixation is ensured by the proper positioning of the implant, based on the appropriate shape of the implant and the quality of bone cut. The initial stability is only temporary, being estimated to last approximately three months. After this period, the secondary stability starts, determined by the bone ingrowth into the implant surface structure. Osteogenic differentiation and extracellular matrix (ECM) mineralization can be enhanced by the presence of bone morphogenetic proteins (BMPs),...

Final thesis deals with research of exploitation of "sur lie" method in production of white wine. The focus is given on stability of wine, especially, stability against crystallization of tartrate salt acid in bottle, proteins stability and antioxidative nature of fine lees. First part of the thesis describes process of wine production by method of aging on fine lees. Composition of lees and cells of Sacharomyces cerevisiae together with its risks and benefits are illustrated in theoretical part. Experimental work monitored development of the wine stability in micro samples. The wine used for research purposes was variety of Silvánske zelené. Antioxidative capacity was monitored by FRAP method in the time of 16 weeks. Stability of proteins was measured by heat protein test and by turbidity meter. Content of tartrate salt was measured by filtration of samples and then weigh on laboratory scales. Sensory analysis was made by blind wine tasting degustation of professional commission. The results confirm higher protein stability which is about 30% more than in control sample. Risk of tartrate salt crystallization was reduced about 80 % in comparison with initial state. Antioxidative capacity of wine demonstrates slight decrease, but real required dose of SO2 added into wine was reduced. Key words: sur lie, bâtonnage, tartrate salt stabilty, antioxidative capacity, fine lees, mannoproteins, yeast cell, protein stability

Cieľom práce je zanalyzovať aktuálny výrobný proces dráh pre hydraulické motory a navrhnúť jeho optimalizáciu. Na základe analýzy výrobného procesu a prieskumu dostupných možností automatizácie sú vytvorené viaceré návrhy automatizovanej výrobnej bunky. Práca sa zaoberá technicko-ekonomickou analýzou projektu a porovnáva viaceré možnosti automatizácie výroby.

Understanding and working with Microsoft Windows registry is an important ability from the perspective of security. This ability is used by malicious software as well as by software, which repaires the damage caused by activity of malicious software. However, applications accessing and working with the registry are platform dependent, which may not always be convenient and it can lead to other problems if the platform is not secure. Therefore, the aim of this work is to create a platform independent application for accessing and working with registry, which makes possible to analyse the effect of malware on registry.

Fast and precise quantification of circulating tumour cells (CTC) in lung adenocarcinoma is a pivotal step in acceleration of diagnosis, selection of early therapy and estimation of treatment prognosis. Development of a new type of microfluidic device based on detection and quantification of epithelial- and mesenchymal-type CTC by high-affinity and cell-type specific protein binders anchored to a microfluidic chip surface represents a highly innovative approach. In this work, we used EpCAM membrane glycoprotein as a target for generation of epithelial cell-specific protein binders by a directed evolution of proteins selected from highly complex combinatorial libraries derived from albumin-binding domain scaffold (ABD) or human muscle protein domain-derived "Myomedin" scaffold. Collections of EpCAM-binding candidates from the both used libraries were generated and particular binding variants were further characterized by DNA sequencing, biochemically and by functional cell-surface binding assays. The best candidates might serve as robust anchor proteins of a microfludic chip. Key words: epithelial cell, EpCAM, protein binder, ribosome display, combinatorial library, protein scaffold

This master’s thesis deals with the design of a robotic cell for the automation of woodworking machine tending. The task of the robot is to manipulate with the specified laminated chipboards of various sizes, before and after machining. The boards are brought to the cell on pallets in three possible placement variants. After a brief research part, conceptual designs of cell layout were created. The optimal and further elaborated design came as a result of selected evaluation criteria. The next part of the thesis consists of designs and selection processes of individual components. These were later used to create a simulation model in Tecnomatix Process Simulate software (Siemens), that helped to verify the functionality of the cell layout, design the control logic and acquire the time of the manipulation cycle used for informative calculation of annual production. In the end, the final design was evaluated from an economic point of view with an emphasis on the return of the initial investment.

Fast and precise quantification of circulating tumour cells (CTC) in lung adenocarcinoma is a pivotal step in acceleration of diagnosis, selection of early therapy and estimation of treatment prognosis. Development of a new type of microfluidic device based on detection and quantification of epithelial- and mesenchymal-type CTC by high-affinity and cell-type specific protein binders anchored to a microfluidic chip surface represents a highly innovative approach. In this work, we used EpCAM membrane glycoprotein as a target for generation of epithelial cell- specific protein binders by a directed evolution of proteins selected from highly complex combinatorial libraries derived from albumin-binding domain scaffold (ABD) or human muscle protein domain-derived "Myomedin" scaffold. Collections of EpCAM-binding candidates from the both used libraries were generated and particular binding variants were further characterized by DNA sequencing, biochemically and by functional cell-surface binding assays. The best candidates might serve as robust anchor proteins of a microfludic chip. Key words: epithelial cell, EpCAM, protein binder, ribosome display, combinatorial library, protein scaffold

The aim of this diploma thesis is to design a cell for robotic arc welding of steel frame. Three possible solutions are provided and one of them is to be chosen to work out. Construction designs of fixture, machine guarding and material racks were created and devices and components required for cell functionality were chosen. All of that with regard to the safety of workplace operators.The result is 3D model of welding cell, it´s implementation and virtual commissioning in the simulation software Process simulate. We will verify the weldability of assigned part, find out the production cycle, eliminate error and lastly the technical and economic evaluation will be work out.

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