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The use of bone morphogenetic proteins and growth factor morphogens in tissue engineering of articular cartilageMotaung, Shirley Caroline Keolebogile January 2009 (has links)
Thesis (DTech. degree in Biomedical Sciences)--Tshwane University of Technology, 2009. / Damaged articular cartilage requires tissue engineering based therapeutic methods
because of its minimal self-repair capacity. Articular cartilage is a heterogeneous
avascular, aneural tissue that provides a smooth bearing surface for movement. It
consisting of superficial, middle, and deep layers with varying matrix composition. The
superficial zone is the site of superficial zone protein synthesis that functions as a
boundary lubricant. The middle and deep layers abound in collagen II and proteoglycan
aggrecan.
Since articular cartilage consists of zones that are specific in their functions, the
replication of these zones may be important in obtaining a functional tissue-engineered
construct. Understanding the differences in gene expression between these layers is
necessary for tissue engineering.
The aim of the study is to use bone morphogenetic proteins (BMPs) and growth factor
morphogens that may help in the regeneration and repair of articular cartilage through
stimulation of chondrocyte proliferation and differentiation. The role of various
transforming growth factor beta (TGF-)/Bone morphogenetic proteins (BMPs)
superfamily on expression of the following proteins: cartilage oligomeric matrix protein
(COMP), superficial zone protein (SZP), cartilage intermediate layer protein (CILP),
SRY-related (sex determining region Y)-sox 9 (SOX 9), aggrecan (AGG) and collagen
type II from three distinct zones of cartilage (superficial, middle and deep) was
investigated.
It is hypothesized that the chondrocytes from the superficial, middle, and deep zone of
articular cartilage would have different rates of proliferation and biosynthetic activity,
and that these proteins would respond differently when treated with the (TGF-)/ bone
morphogenetic proteins (BMPs) superfamily.
Stifle joints (n=28) from three month-old calves were obtained from a local abattoir.
Articular chondrocytes were isolated from three distinct zones of cartilage and cultured
as monolayers in a serum-free chemically defined medium. The results are presented
as the mean and standard deviations for each experiment. A one way analysis of
variance (ANOVA) with Tukey’s HSD (Honestly Significant Differences) to account for
multiple comparisons was used to determine the effects TGF- _1, BMP-7, GDF-5, and
Activins A, B and AB isoforms on COMP, SZP, CILP, aggrecan, sox 9 and collagen type
II accumulation in different zones of articular cartilage. A significance level of p < 0.05
was used to determine the difference between the control and treated cells and p < 0.01
the dose-dependence of morphogens within the zones.
Accumulation of COMP, SZP, CILP, aggrecan, sox 9 and collagen type II in culture
medium in response to various members of the BMP/TGF-_ superfamily was
determined by enzyme-linked immunosorbent assay (ELISA) and messenger
ribonucleic acid (mRNA) by quantitative real-time PCR. TGF-_1 significantly stimulated
the expression of COMP and SZP at the mRNA and protein level in the superficial zone
in a time- and dosage- dependent manner, CILP at the mRNA in the middle zone,
aggrecan, sox 9 and collagen type II at the mRNA in the middle and deep zone. An
unexpected discovery was that the superficial chondrocytes were more responsive to
morphogens than the deeper layers. BMP-7 and growth differentiation factors (GDF-5)
also up-regulate COMP, SZP, CILP, aggrecan and collagen type II expression, although
the effects were not as potent as with TGF-_1. Activins A, B and AB demonstrated no
effects on all the genes accumulation in all zones. In conclusion, TGF-_, BMP-7 and
GDF-5 are important regulators of different genes in different zones of articular
cartilage.
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Functional genomic analyses of the impact of global hypomethylation and of tumor microenvironment in a rat model of human chondrosarcomaHamm, Christopher Allan. Soares, Marcelo B. January 2009 (has links)
Thesis supervisor: Marcelo B. Soares. Includes separate files (Appendix A-E) for thesis supplements. Includes bibliographic references (p. 102-115).
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Three-dimensional chondrocyte culture : in vitro and in vivo applications /Cook, James L. January 1998 (has links)
Thesis (Ph. D.)--University of Missouri--Columbia, 1998. / "December 1998" Typescript. Vita. Includes bibliographical references (l. 132). Also available on the Internet.
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Three-dimensional chondrocyte culture in vitro and in vivo applications /Cook, James L. January 1998 (has links)
Thesis (Ph. D.)--University of Missouri--Columbia, 1998. / Typescript. Vita. Includes bibliographical references (leaf 132). Also available on the Internet.
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Chondrocyte behavior in monolayer culture : the effects of protein substrates and culture mediaBrodkin, Kathryn Rhea 12 1900 (has links)
No description available.
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Molecular pathogenesis of abnormal chondrocyte differentiation in a transgenic mouse model /Tsang, Kwok-yeung. January 2006 (has links)
Thesis (Ph. D.)--University of Hong Kong, 2006.
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Chondrocyte death in the development of osteoarthritis in STR/ort miceMistry, Deepika January 2002 (has links)
No description available.
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Modeling of articular cartilage optimization, large deformation, and microstructure /Lei, Fulin. January 2006 (has links)
Thesis (Ph.D.)--University of Delaware, 2006. / Principal faculty advisor: Andras Z. Szeri, Dept. of Mechanical Engineering. Includes bibliographical references.
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Development and design of a test device for cartilage wear studies /Burkhardt, Bettina M., January 1988 (has links)
Thesis (M.S.)--Virginia Polytechnic Institute and State University, 1988. / Vita. Abstract. Includes bibliographical references (leaves 116-125). Also available via the Internet.
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Morphologische Untersuchungen über die Entwicklung des Nasenknorpels bei MäuseembryonenGoetzel, Raymond, January 1980 (has links)
Thesis (doctoral)--Freie Universität Berlin, 1980.
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