No description available.

Thesis (M.S.)--University of Delaware, 2008. / Principal faculty advisor: Ulhas Naik, Dept. of Biological Sciences. Includes bibliographical references.

Thesis supervisor: Joshua A. Weiner. Includes bibliographic references (p. 108-126).

Cell adhesion molecules enhance Interactions between adjacent cells In order to mediate a large variety of functions of the Immune system. An antibody against the murine lymphocyte surface antigen MALA-2 has previously been shown to Inhibit mixed lymphocyte response. A λgt10 cDNA library from NS-1 cells was screened and a cDNA clone, K3-1.1, was previously isolated. It had significant homology to the human ICAM-1 gene. This thesis covers the isolation of a second cDNA clone, K4-1.1, and its comparison to K3-1.1 In terms of expression, function and distribution. The two clones are identical in sequence with the exception of the 5’ ends. Expression of these two clones was examined using a transient expression system of COS cell transfection. Cell surface expression of the K3-1.1 clone could not be detected by FACS analysis. Even when the 5' untranslated region of the K3-1.1 clone (which has 10 potential translation start sites) was removed, protein could not be detected at the cell surface, intracellularly, or extracellularly. However, K4-1.1 expression was detected at the cell surface. Northern blot analysis reveals that there are two distinct messages which are likely to be represented by the two clones. When the northern blot was probed with the 5' end of the K3-1.1 clone, only one of the messages was detected. This together with the result of Southern blot analysis suggests that the two messages are likely the result of alternate splicing. In order to examine the interactions of the murine ICAM-1 with the surface of other cells, an expression system which would produce large amounts of a secreted soluble form was established. The soluble protein was purified from the supernatant of transfected cells by an antibody-affinity column and used in preliminary binding assays. / Medicine, Faculty of / Medical Genetics, Department of / Graduate

No description available.

Thesis (Ph. D.)--University of Chicago, Dept. of Biochemistry and Molecular Biology, June 1999. / Includes bibliographical references. Also available on the Internet.

published_or_final_version / Medical Sciences / Master / Master of Medical Sciences

Erratum is pasted on back end paper. / Bibliography: leaves 231-268. / xv, 268 leaves : ill. (chiefly col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / This thesis examines the expression of cell adhesion molecules on both endothelial cells (ECs) and circulating leukocytes, and investigates the role of these molecules in renal allograft rejection. The aims of the study are to establish an ovine model of renal allograft transplantation, to generate monoclonal antibodies (mAbs) against ovine endothelial cell adhesion molecules, to examine the efficacy of the mAbs in prolonging the survival of the sheep renal allografts and to study the kinetics of expression of cell adhesion molecules (CAMs) during cellular activation 'in vitro' and 'in vivo' during the onset of allograft rejection. / Thesis (Ph.D.)--University of Adelaide, Dept. of Medicine, 1996

Includes bibliographical references (leaves 281-318) / xxv, 318 leaves : ill. (some col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Characterises the antigen recognized by mAb UA009 and investigates whether it is an endothelial adhesion marker. The identification of a CD36-like molecule led to targeted histological studies on the expression of the molecule in specific tissues of interest. / Thesis (Ph.D.)--Adelaide University, Dept. of Microbiology and Immunology, 2001

Thesis (M. Med. Sc.)--University of Hong Kong, 2000. / Includes bibliographical references (leaves 82-86).