Submitted by Anaclaudia Mattos Villalba (anaclaudiamattosvillalba@gmail.com) on 2016-04-09T23:17:42Z No. of bitstreams: 1 youblisher.com-1121435-Disserta_o_Juliana_do_Prado_Alves.pdf: 930829 bytes, checksum: 7e45b5c57610f7484528d8a3a062676f (MD5) / Approved for entry into archive by cleuza maria medina dos santos (cleuzamai@yahoo.com.br) on 2016-05-09T13:32:46Z (GMT) No. of bitstreams: 1 youblisher.com-1121435-Disserta_o_Juliana_do_Prado_Alves.pdf: 930829 bytes, checksum: 7e45b5c57610f7484528d8a3a062676f (MD5) / Made available in DSpace on 2016-05-09T13:32:46Z (GMT). No. of bitstreams: 1 youblisher.com-1121435-Disserta_o_Juliana_do_Prado_Alves.pdf: 930829 bytes, checksum: 7e45b5c57610f7484528d8a3a062676f (MD5) Previous issue date: 2014 / Os ecossistemas aquáticos continentais são um exemplo de ecossistema de água doce, compreendendo rios, reservatórios, lagoas e áreas alagáveis. A planície costeira do Rio Grande do Sul possui três grandes corpos d´água continentais, dentre eles a Lagoa Mangueira é a terceira maior. Na Lagoa Mangueira encontra-se varias espécies de peixes, o Odontesthes bonariensis (peixe-rei) está entre as espécies mais abundantes da Lagoa. Essa espécie é nativa tendo importância no fluxo de nutrientes e nas estruturas tróficas do ambiente, além da grande importância econômica na região. Uma das possibilidades de manter a variabilidade genética, possibilitando proteger ou até mesmo recuperar espécies de peixe-rei, é a técnica de criopreservação seminal que consiste em resfriar e/ou congelar espermatozoides, utilizando diluentes e crioprotetores específicos. O presente estudo testou três tipos de amidas, dimetilacetamida (DMA), dimetilformamida (DMF) e metilformamida (MF) nas concentrações de 2, 5, 8 e 11%, tendo como controle o dimetilsulfoxido na concentração de 10% (DMSO). Cada tratamento foi diluído em Mounib modificado. Foi avaliada a motilidade (taxa e tempo) e as estruturas celulares (integridade de membrana plasmática, funcionalidade de mitocôndria, índice de fragmentação de DNA e fluidez de membrana plasmática). O DMSO 10% e a MF 8% obtiveram melhores resultados em relação à integridade de membrana plasmática (34, 8% e 29%), funcionalidade de mitocôndria (19,8% e 16,2%) e fluidez de membrana plasmática (39,4% e 46,4%), além disso, a taxa de motilidade (11,8% e 10%) e o tempo de motilidade (81,4 seg. e 71,8 seg.) mantiveram-se entre as melhores médias. Dessa forma, considerando os parâmetros avaliados, o DMSO10% e a MF8% foram associados à melhor criopreservação das células espermáticas de O. bonariensis. / The freshwater ecosystems are an example of freshwater ecosystem, comprising rivers, reservoirs, ponds and wetlands. The coastal plain of Rio Grande do Sul has three major continental water bodies, including the Mangueira Lagoon is the third largest. In Mangueira Lagoon is several species of fish, the Odontesthes bonariensis (pejerrey) is among the most abundant species of Lagoa. This species is native having importance in the flow of nutrients and trophic structures in the environment, in addition to the great economic importance in the region. One possibility to maintain genetic variability, enabling protect or even recover species of kingfish, is the seminal cryopreservation technique of cooling and / or freeze sperm, using specific diluents and cryoprotectants. The present study tested three kinds of amides, dimethylacetamide (DMA), dimethylformamide (DMF) and dimethylformamide (MF) at concentrations of 2, 5, 8 and 11%, with the control dimethylsulfoxide at a concentration of 10% (DMSO). Each treatment was diluted in modified Mounib. Motility was assessed (rate and time), and cell structures (plasma membrane integrity, mitochondrial function, DNA fragmentation index and fluidity of the plasma membrane). The 10% DMSO and MF 8% did better on the completeness of the plasma membrane (34.8% and 29%), functional mitochondria (19.8% and 16.2%) and plasma membrane fluidity (39.4% and 46.4%), furthermore, the motility rates (11.8% and 10%) and the motility of time (81.4 s. and 71.8 s.) remained among the best averages. Thus, considering the parameters evaluated, the DMSO10% and MF8% were associated with better cryopreservation of sperm cells of O. bonariensis.

Global climate change has been a point of concern over the past century. Some of its major consequences, which are already present, include melting of glaciers, increasing sea water level, temperature rise and excessive acidity of sea water. The natural fluctuations harm the ecology and the biological species will face increased extinction risk. The raise of the water level will cause sinking and gradually vanishing of the land’s surface as a natural resource and place for habitation. It has been estimated that if Greenland ice sheet melts completely, the water would be enough to cover the land with up to 6 meters. The project investigates the consequences of the rising sea levels due to the climate change and what impact this will have on the topography and the natural landscape. It proposes a utopian vision for a large scale strategy for agriculture which does not rely on the use of land. The structure comprises of inflatable spherical modules, which float on the water surface. It is a dynamic and expandable system, with minimal environmental footprint, designed for low-lying areas vulnerable to flooding and land shortage. The more the land surface is vanishing due to the increasing sea levels, the more the structure will stretch to compensate for the loss of farmland.

No description available.

Presented dissertation discusses the influence of electromagnetic fields on the weight of the particles, which are considered in this work as substances with the hub. Theoretical knowledge was practically tested on plant organisms (early somatic embryos, fungal pathogens), theoretically the influence of electromagnetic field on cell structure was modeled. It was subsequently confirmed by a practical experiment assumption about the influence of electromagnetic fields on the weight of the particles. These experiments were preceded by a theoretical study of the problem and the formulation of a solution using Maxwell's equations, from which other descriptive equations and formulas were derived. The results of the experiments were presented at several professional conferences and published in professional journals and proceedings.