Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Study program: Pharmacy Author: Simona Novotná Supervisor: RNDr. Klára Konečná, Ph.D. Title of diploma thesis: Advanced antibacterial activity testing of candidate newly synthesized compounds Background: The aim of this thesis was to perform an extended study of the antibacterial activity of selected newly synthesized rhodanine derivatives. In this study, activity against clinical isolates of bacterial strains of the genus Staphylococcus and Enterococcus was evaluated. The main part of the work also includes the evaluation of the antibacterial activity of one selected substance in combination with commercially available antibiotics using the checkerboard method. Methods: Evaluation of the antibacterial activity of tested substances was performed using the broth microdilution method according to EUCAST guidelines (with minor modifications). The activity of these substances was evaluated against clinical isolates of bacteria of the genera Staphylococcus and Enterococcus and one Staphylococcus aureus MRSA reference strain (ATCC 43300, CCM 4750). For a selected compound with a demonstrably promising antistaphylococcal effect, the combined effect of this substance was tested with three...

The deals of thesis were described methods for designing safety applications, made analysis of possible microcontroller faults of long-run system, described software and hardware methods for fault detection in microcontroller and applied some March test to microcontroller. To application were chosen MATS+, PMOVI and March SS tests. These tests were modified to word-oriented memory. Further it was made analysis of modified tests to determination fault coverage, testing times and program memory requirement. To determination of fault coverage was created virtual memory with fault function models. March tests were compared with each other and with another pattern test (checkboard test).