Maintaining genomic integrity is crucial for cell survival. In Escherichia coli, Rec-Amediated homologous recombination (HR) plays an essential role in the repair of DNA double-strand breaks (DSB) and the SOS response through a series of highly dynamic interactions with the chromosome. A greater understanding of the mechanism of homologous recombination requires quantitative analysis of genomic studies in live cells. The aim of this thesis was to investigate the dynamics of the RecA-DNA interactions in vivo following the induction of a site-specific DSB in the chromosome of E. coli. This DSB is caused by the cleavage of a DNA hairpin by the hairpin-specific endonuclease, SbcCD. The DNA hairpin is formed only on the lagging strand template of replication by a 246 bp-interrupted palindrome. As a result cleavage only occurs on one sister chromosome, leaving one unbroken chromosome to serve as a template for repair by HR. Here, this system has been used as a basis to develop a method that combines chromatin immunoprecipitation with quantitative PCR (ChIP-qPCR) and next-generation sequencing (ChIP-Seq) to quantify RecA protein binding during the active repair of a single chromosomal DSB. This study reports that DSB-dependent RecA binding is stimulated in response to the eight base DNA sequence Chi (5’-GCTGGTGG-3’). Increasing the number of Chi sites close to the DSB stimulates more RecA loading to DNA, with ChIP-Seq analysis also revealing a role for subsequent Chi sites in RecA binding during DSBR. If the Chi sites close to the DSB are removed then Chi-dependent RecA binding to DNA can be observed at distances greater than 100 kb from the DSB, suggesting that these subsequent Chi sites can be engaged in DSBR. Through collaboration, these in vivo data were combined with stochastic modeling to determine that, in vivo, Chi is recognised by the RecBCD complex with an efficiency of 20- 35%. The genomic analysis also revealed two unexpected aspects of RecA protein binding. First, ChIP-Seq analyses identified that following a DSB at lacZ there is RecA enrichment detected in the terminus region of the E. coli chromosome. This RecA binding is Chi-dependent, indicating a role for HR. Second, DSB-independent binding was observed at the RNA encoding genes dispersed throughout the chromosome. A temporal analysis of RecA dynamics was also performed. These analyses revealed that RecA binding to DNA near the DSB is extremely dynamic, cycling between periods of high RecA enrichment and periods of low RecA enrichment. This is the first in vivo study of DSB-dependent RecA-DNA distribution and dynamics in recombination proficient E. coli cells.

Submitted by Sara Ribeiro (sara.ribeiro@ucb.br) on 2018-03-19T21:17:30Z No. of bitstreams: 1 NicoleBacellarZanetiTese2017.pdf: 3968663 bytes, checksum: ec2715db7ea51e10be127e391f9299ee (MD5) / Approved for entry into archive by Sara Ribeiro (sara.ribeiro@ucb.br) on 2018-03-19T21:17:59Z (GMT) No. of bitstreams: 1 NicoleBacellarZanetiTese2017.pdf: 3968663 bytes, checksum: ec2715db7ea51e10be127e391f9299ee (MD5) / Made available in DSpace on 2018-03-19T21:17:59Z (GMT). No. of bitstreams: 1 NicoleBacellarZanetiTese2017.pdf: 3968663 bytes, checksum: ec2715db7ea51e10be127e391f9299ee (MD5) Previous issue date: 2017-09-09 / This thesis discusses spirituality and sexuality, linking them to femininity, in a study of the familiarity of both phenomena by women practitioners of Tai Chi Chuan, also for investigating how they undergo and establish relationships between them. The study was grounded on the husserlian and merleaupontyan phenomenology and foucaultian approach. The methodology was inspired by phenomenology, allowing an exercise of understanding the convergences and divergences in the experiences of six Brazilian women and six British women practitioners of Tai Chi Chuan, whose semi-structured interviews were recorded and later transcribed. In the face of a comprehensive, contextualized and comparative perspective between the two groups, the results were systematized and analyzed according to the empirical-phenomenological method proposed by Amedeu Giorgi. It was possible to understand that the practices of the interviewees in Tai Chi Chuan are related to the search for meaning, as they attach a greater sense of integration to their existence after practicing it than previously reported. The body plays a key role in this integration. According to the merleaupontyan approach, it is a totality, a commonplace in which sexuality and spirituality express themselves. The modulating role of religiosity in the experience of sexuality was also verified. The empirical investigation allowed, therefore, one to investigate and understand how the interviewees meet the dimensions of sexuality and spirituality and establish relations between these two aspects of their psychic life in an in-depth way. Tai Chi Chuan has proved to be a practice capable of assisting the development of a harmonious and balanced coexistence between sexuality and spirituality. / Esta tese discute espiritualidade e sexualidade, relacionando-as com a feminilidade, num estudo da viv??ncia de ambos os fen??menos por parte de mulheres praticantes de Tai Chi Chuan, investigando o modo como elas os experimentam e estabelecem rela????es entre eles. O estudo fundamentou-se na fenomenologia husserliana e merleaupontyana e na abordagem foucaultiana. A metodologia foi inspirada na fenomenologia, permitindo exerc??cio de compreens??o das converg??ncias e diverg??ncias nas experi??ncias de seis mulheres brasileiras e seis mulheres brit??nicas que praticam Tai Chi Chuan, com as quais realizou-se entrevistas semiestruturadas, gravadas e posteriormente transcritas. Os resultados foram sistematizados e analisados segundo o m??todo emp??rico-fenomenol??gico proposto por Amedeu Giorgi, numa perspectiva compreensiva, contextualizada e comparativa entre os dois grupos. Foi poss??vel compreender que as viv??ncias das entrevistadas no Tai Chi Chuan est??o relacionadas ?? busca de sentido, pois elas atribuem um sentido maior de integra????o ?? sua exist??ncia depois de pratic??-lo do que atribu??am antes. O corpo desempenha papel fundamental nesta integra????o. Segundo a perspectiva merleaupontyana, ?? uma totalidade, lugar comum em que a sexualidade e a espiritualidade se expressam. Verificou-se tamb??m o papel modulador da religiosidade na viv??ncia da sexualidade. A investiga????o emp??rica permitiu, portanto, conhecer como as entrevistadas experimentam as dimens??es da sexualidade e da espiritualidade e estabelecem rela????es entre esses dois aspectos de sua vida ps??quica de maneira aprofundada. O Tai Chia Chuan mostrou-se como uma pr??tica capaz de auxiliar o desenvolvimento de uma conviv??ncia harmoniosa e equilibrada entre sexualidade e espiritualidade.

Xu Dongqing. / "June 2003." / Thesis (Ph.D.)--Chinese University of Hong Kong, 2003. / Includes bibliographical references (p. 140-154). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Electronic reproduction. Ann Arbor, MI : ProQuest Information and Learning Company, [200-] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Mode of access: World Wide Web. / Abstracts in English and Chinese.

In 1986, Vietnam opened up the country’s economy by launching the Doi Moi (renovation) policy, which made the process of economic liberalisation possible. Accordingly, Ho Chi Minh City, the country’s biggest city, has undergone rapid growth of population, urbanisation and industrialisation. While achieving remarkable economic growth, the city faces considerable urban environmental challenges. The rapid growth of urbanisation and industry has placed increasing pressure on available freshwater resources, through excessive water use and increasing volumes of untreated wastewater. Firstly, the urban water sector has had to meet growing demand for water use for domestic purposes during the dry season. Secondly, groundwater levels have experienced a substantial drawdown in outlying urban districts as a result of over-abstraction. Thirdly, rapidly expanding industrial activities are causing severe demand on water resources. The city’s major supply sources have developed an alarming level of pollutants discharged by industry. The aim of this thesis is to formulate an integrated approach to the sustainable management of water resources in Ho Chi Minh City. In order to achieve this, the research was designed to examine institutional frameworks and arrangements and to explore the perception of water value by water users and stakeholders. The underlying reasons for ineffective management are anchored in fragmented management practices which result from inadequate institutional frameworks and arrangements, inadequate regulations and inappropriate water governance. Weak enforcement of law and insufficient cooperation between government agencies and departments in Ho Chi Minh City and their counterparts in neighbouring provinces also limit management efficacy. The research results show that stakeholders have different perceptions of water resources. Overall, water value has been considered as a social and economic good by both the urban respondents and government officials. However, most urban residents view water as a social good rather than an economic one. Public involvement in the water sector is limited. Most urban dwellers have little understanding and knowledge about the city’s water issues or the available channels to access information on water resources. Many government officials are inadequately trained, poorly qualified, inexperienced and have irrelevant or outdated background knowledge about their field of management. Government respondents did not provide consistent data and information on the water profile because there is no shared common information on water issues in place. Findings from the fieldwork show that decentralisation, privatisation and using rainwater as a potential alternative water source are preferred. Finally, the study proposes a schematic revision of existing management structures and mechanisms between local government agencies. This thesis proposes a model for a water conservation strategy for which the management and use of water resources is aligned with adequate institutional arrangements and effective regulations. Water governance and management of water resources need to work with economic and urbanisation growth. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1331639 / Thesis (Ph.D.) - University of Adelaide, School of Social Sciences, 2008

In 1986, Vietnam opened up the country’s economy by launching the Doi Moi (renovation) policy, which made the process of economic liberalisation possible. Accordingly, Ho Chi Minh City, the country’s biggest city, has undergone rapid growth of population, urbanisation and industrialisation. While achieving remarkable economic growth, the city faces considerable urban environmental challenges. The rapid growth of urbanisation and industry has placed increasing pressure on available freshwater resources, through excessive water use and increasing volumes of untreated wastewater. Firstly, the urban water sector has had to meet growing demand for water use for domestic purposes during the dry season. Secondly, groundwater levels have experienced a substantial drawdown in outlying urban districts as a result of over-abstraction. Thirdly, rapidly expanding industrial activities are causing severe demand on water resources. The city’s major supply sources have developed an alarming level of pollutants discharged by industry. The aim of this thesis is to formulate an integrated approach to the sustainable management of water resources in Ho Chi Minh City. In order to achieve this, the research was designed to examine institutional frameworks and arrangements and to explore the perception of water value by water users and stakeholders. The underlying reasons for ineffective management are anchored in fragmented management practices which result from inadequate institutional frameworks and arrangements, inadequate regulations and inappropriate water governance. Weak enforcement of law and insufficient cooperation between government agencies and departments in Ho Chi Minh City and their counterparts in neighbouring provinces also limit management efficacy. The research results show that stakeholders have different perceptions of water resources. Overall, water value has been considered as a social and economic good by both the urban respondents and government officials. However, most urban residents view water as a social good rather than an economic one. Public involvement in the water sector is limited. Most urban dwellers have little understanding and knowledge about the city’s water issues or the available channels to access information on water resources. Many government officials are inadequately trained, poorly qualified, inexperienced and have irrelevant or outdated background knowledge about their field of management. Government respondents did not provide consistent data and information on the water profile because there is no shared common information on water issues in place. Findings from the fieldwork show that decentralisation, privatisation and using rainwater as a potential alternative water source are preferred. Finally, the study proposes a schematic revision of existing management structures and mechanisms between local government agencies. This thesis proposes a model for a water conservation strategy for which the management and use of water resources is aligned with adequate institutional arrangements and effective regulations. Water governance and management of water resources need to work with economic and urbanisation growth. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1331639 / Thesis (Ph.D.) - University of Adelaide, School of Social Sciences, 2008

In 1986, Vietnam opened up the country’s economy by launching the Doi Moi (renovation) policy, which made the process of economic liberalisation possible. Accordingly, Ho Chi Minh City, the country’s biggest city, has undergone rapid growth of population, urbanisation and industrialisation. While achieving remarkable economic growth, the city faces considerable urban environmental challenges. The rapid growth of urbanisation and industry has placed increasing pressure on available freshwater resources, through excessive water use and increasing volumes of untreated wastewater. Firstly, the urban water sector has had to meet growing demand for water use for domestic purposes during the dry season. Secondly, groundwater levels have experienced a substantial drawdown in outlying urban districts as a result of over-abstraction. Thirdly, rapidly expanding industrial activities are causing severe demand on water resources. The city’s major supply sources have developed an alarming level of pollutants discharged by industry. The aim of this thesis is to formulate an integrated approach to the sustainable management of water resources in Ho Chi Minh City. In order to achieve this, the research was designed to examine institutional frameworks and arrangements and to explore the perception of water value by water users and stakeholders. The underlying reasons for ineffective management are anchored in fragmented management practices which result from inadequate institutional frameworks and arrangements, inadequate regulations and inappropriate water governance. Weak enforcement of law and insufficient cooperation between government agencies and departments in Ho Chi Minh City and their counterparts in neighbouring provinces also limit management efficacy. The research results show that stakeholders have different perceptions of water resources. Overall, water value has been considered as a social and economic good by both the urban respondents and government officials. However, most urban residents view water as a social good rather than an economic one. Public involvement in the water sector is limited. Most urban dwellers have little understanding and knowledge about the city’s water issues or the available channels to access information on water resources. Many government officials are inadequately trained, poorly qualified, inexperienced and have irrelevant or outdated background knowledge about their field of management. Government respondents did not provide consistent data and information on the water profile because there is no shared common information on water issues in place. Findings from the fieldwork show that decentralisation, privatisation and using rainwater as a potential alternative water source are preferred. Finally, the study proposes a schematic revision of existing management structures and mechanisms between local government agencies. This thesis proposes a model for a water conservation strategy for which the management and use of water resources is aligned with adequate institutional arrangements and effective regulations. Water governance and management of water resources need to work with economic and urbanisation growth. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1331639 / Thesis (Ph.D.) - University of Adelaide, School of Social Sciences, 2008

<p>Syfte: Syftet med denna uppsats är att undersöka om det finns något samband mellan intellektuellt kapital och substansmultipeln. Undersökningen omfattade 81 av de största Nordiska börsföretagen sett till omsättning och marknadsvärde under perioden 2001 till 2005.</p><p>Metod: Vi har använt oss av ett deduktivt tillvägagångssätt då vi studerat årsredovisningar och utifrån dessa försökt finna belägg för vårt antagande om sambandet mellan intellektuellt kapital och substansmultipeln.</p><p>Resultat/Slutsatser:</p><p>Det är inte statistiskt säkerhetsställt att det finns ett samband mellan intellektuellt kapital och substansmultipeln. Det kan finnas förklaringar till resultatet i de ord som valts att basera undersökningen på och om de ska rangordnas eller inte.</p>

Syfte: Syftet med denna uppsats är att undersöka om det finns något samband mellan intellektuellt kapital och substansmultipeln. Undersökningen omfattade 81 av de största Nordiska börsföretagen sett till omsättning och marknadsvärde under perioden 2001 till 2005. Metod: Vi har använt oss av ett deduktivt tillvägagångssätt då vi studerat årsredovisningar och utifrån dessa försökt finna belägg för vårt antagande om sambandet mellan intellektuellt kapital och substansmultipeln. Resultat/Slutsatser: Det är inte statistiskt säkerhetsställt att det finns ett samband mellan intellektuellt kapital och substansmultipeln. Det kan finnas förklaringar till resultatet i de ord som valts att basera undersökningen på och om de ska rangordnas eller inte.

Falls among Canadian older adults is a growing problem, not only in terms of incident rates and its impacts, but most importantly, the vast scope of preventable morbidity and mortality. A decline in age-related physical function is acknowledged as a risk factor of falls. Community-based group exercise programs such as Yang style tai chi may help to reduce fall risk by improving components of physical function. To investigate, a pretest-posttest experimental design was conducted. Post-intervention assessments revealed Yang style tai chi practiced three times a week over eight weeks improved balance, muscle strength and muscle endurance. These findings suggest Yang style tai chi is moderately effective in reducing fall risk but further research is needed to determine the true effectiveness of tai chi as a fall prevention strategy. This study provides directions for future research guided by a proposed conceptual framework and offers some tentative recommendations for community health practice. / UOIT

Cognitive impairment is prevalent among older adults population. It brings restriction to older adults’ lives and bring huge burden to the society. Mind-body exercise has characteristics from both physical exercise and intellectual experience. Moreover, it has potential cognitive benefits to reduce the incidence even reverse cognitive impairment. Using meta-analysis to analyze findings form published research on mind-body exercise, this study will explore whether practicing mind-body exercise is beneficial for the management of cognitive impairment.