CATD was not only adequate for analyzing the pursuit of erratically moving prey but also worked well when analyzing the pursuit of prey that remained stationary. It cannot be fully concluded that bats utilize the CATD strategy to successfully capture erratically flying prey. The angle remains relatively constant but does not exhibit a zero change in angle as by definition. The large forces experienced by the bat were seen when the bat began to rotate its body about its pitch axis or when the bat made a large turn. Moments were seen specifically when the bat began to bank into its last and final turn towards its target.; Digital recordings of three different species of bats were studied in this thesis to determine the forces and moments that were experienced throughout the bat's flight. The recordings were also studied to determine the pursuit strategies that were most effective for the bat to quickly capture its prey. A pursuit strategy is a strategic way to travel that will allow a pursuer to capture/approach their target the quickest. Therefore when a bat utilizes a particular pursuit strategy, it will adjust its position/ direction vector in a particular way that will allow it to approach its target very quickly. Data was collected directly from the video by manual collection utilizing Microsoft Visual Studio to extract frames, collect and record the data. This research was conducted to determine when throughout the flight the bat would experience significant forces and moments. The location and magnitude of the forces were reported along with an explanation of why the bat was experiencing a peak at each specific time. The forces and moments that the bat experienced thought-out the flight pursuit were calculated by relative velocity and acceleration calculations. In all four scenarios the bat experienced forces in relation to rotating its body about its center of mass. Forces were specifically seen when the bat periodically began to rotate its body before the final plunge to capture its prey. Prey avoidance and pursuit strategies were also studied and observed in this thesis which included the constant bearing and the constant absolute target direction. The intent was to determine which pursuit strategy bats use to quickly capture their prey. The constant bearing strategy is utilized to pursue prey moving along a smooth path, on the other hand the Constant Absolute Target Direction (CATD) pursuit strategy is utilized to capture erratically moving prey. For most of the bats analyzed, it was seen that the CATD strategy proved to be the preferred pursuit strategy.

In recent years, the demand for Li-ion-type batteries has been increasing significantly in various fields of applications including portable electronics, electric vehicles, and also in renewable energy support. These applications ask for a highly efficient charging strategy in order to maintain a long life cycle of the batteries. Recently, a new charging technique referred as sinusoidal ripple current-constant voltage charging (SRC-CV) technique has been proposed and is in certain publications claimed to realize an improved charging per-formance on Li-ion batteries than conventional constant-current constant-voltage charg-ing (CC-CV) techniques. In this thesis, the charging performance of the SRC-CV charging method applied to a prismatic Li-ion cell for an automotive traction application is inves-tigated. An existing experimental setup is upgraded to realize charging of the Li-ion cells using the SRC-CV charging method. Electrochemical impedance spectrums of three Li-ion cells have been obtained using electrochemical impedance spectroscopy (EIS). These spectrums were used to determine the charging ripple-current frequency where the mag-nitudes of the ac impedance of the cell are minimized. Key parameters like charging time, discharging time, and energy efficiency are calculated in order to compare the charg-ing performance of the CC-CV and SRC-CV charging techniques. The results reported from the experimental results obtained in this thesis indicate that there is no significant improvement with the SRC-CV charging method (implemented using a constant ripple-current frequency) compared to the CC-CV method in terms of charging time and energy efficiency. / På senare tid har behovet av batterier av Li-jontyp ökat kraftigt inom ett flertal applikationsområden inkluderande portabel elektronik, elfordon och miljövänlig elenergiproduktion. I dessa applikationsområden behövs en högeffektiv laddstrategi för att möjliggöra ett stort antal cyklingar av batterierna. Nyligen har en new laddmetod, benämnd sinusoidal ripple current-constant voltage-laddning (SRC-CV-laddning) föreslagits och har i vissa publikationer demonsterat en förbättring av laddprestanda hos Li-jonbatterier jämfört med konventionell constant-current constant-voltage-laddning (CC-CV-laddning). I detta examensarbete undersöks laddprestandan hos SRC-CV och CC-CV-laddning när de appliceras på prismatiska Li-jonceller avsedda för traktionsdrift. En existerande experimentuppsättning har uppgraderats för att realisera laddcykling med SRC-CV-laddning. Med hjälp av elektrokemisk impedansspektroskopi på tre Li-jonceller har den frekvens vid vilken magnituden på cellernas impedans är minimerad identifierats. Nyckelparametrar såsom laddtid, urladdningstid och energieffektivitet har uppmätts för både SRC-CV- och CC-CV-laddning. De experimentella resultaten visar ingen signifikant förbättring mellan SRC-CV-laddning (implementerat med en konstant rippelströmfrekvens) och konventionell CC-CV-laddning.

The increasing commercial and industrial use of ethanol, i.e., in biofuel and gasoline, has generated increased incidents of vadose zone contamination by way of ethanol spills and releases. This has increased the interest in better understanding infiltration behaviours of ethanol in unsaturated porous media and the multiphase interactions in the vadose zone. Solute-dependent capillarity-induced focused flow (SCIFF) is a vertical, highly focused flow infiltration behaviour first reported by Smith et al. (2011) in butanol-contaminated sands. Through the use of highly controlled laboratory experiments, this thesis research investigates focused flow (SCIFF) and related behaviours through water infiltration into ethanol-contaminated unsaturated sand. Focused flow behaviours (SCIFF) were demonstrated through the infiltration of water into an ethanol-contaminated unsaturated sand using both constant flux and constant head methodologies. The observation of focused flow behaviours in ethanol-contaminated sand supported the primary hypothesis of this work. The secondary hypothesis was also supported, as focused flow behaviours were not observed, rather stable semicircular infiltration patterns were observed during ethanol infiltration into water-wet sand. Comparisons between constant flux and constant head application methods under similar flow rates and fluid volumes produced similar results. The zone of lower saturation, or the “halo effect” reported in previous literature, was strongly expressed during water infiltration in ethanol-contaminated sand. This halo effect is affected by the maximum (at 40% to 50%) of aqueous concentration of ethanol. This maximum enhances the zone of lower saturation and stabilizes the solute front. The SCIFF focused flow also overcame the effects of minor heterogeneities in the sand. However, additional laboratory and modelling work is required to further understand the extent of SCIFF behaviour. / Thesis / Master of Science (MSc) / Understanding the behaviour and interaction of water and contaminants in soils is important as environmental contamination and spills can have devastating environmental impacts. In recent decades, ethanol spills and accidental releases onto ground surface have increased as the commercial and industrial use of ethanol has increased. The goals of this work were to qualitatively visualize and quantify the unique nature of water infiltration into the ethanol-contaminated soil and understand the complex mechanisms behind water-ethanol interactions. This research showed that water infiltration creates an uncommon vertical, focused pattern when flowing into sand contaminated by ethanol. However, when ethanol is applied to standard water-wet sand, that behaviour is not observed. This work provided greater insight into the nature of ethanol-contaminated soils. These findings furthered the understanding needed to evaluate impacts that ethanol contamination can have on remedial efforts and the rate of migration of contaminants to groundwater.

The critically important nature of radical and radical ion mechanisms in biology and chemistry continues to be recognized as our understanding of these unique transient species grows. The work presented herein demonstrates the versatility of kinetic studies for understanding the elementary chemical reactions of radicals and radical ions. Chapter 2 discusses the use of direct ultrafast kinetics techniques for investigation of crucially important enzymatic systems; while Chapter 3 demonstrates the value of indirect competition kinetics techniques for development of synthetic methodologies for commercially valuable classes of compounds. The mechanism of decay for aminyl radical cations has received considerable attention because of their suspected role as intermediates in the oxidation of tertiary amines by monoamine oxygenases and the cytochrome P450 family of enzymes. Radical cations are believed to undergo deprotonation as a key step in catalysis. KIE studies performed by previous researchers indicate N,N-dimethylaniline radical cations deprotonate in the presence of the bases acetate and pyridine. By studying the electrochemical kinetics of the reaction of para substituted N,N-dimethylaniline radical cations with acetate anion, we have produced compelling evidence to the contrary. Rather than deprotonation, acetate reacts with N,N-dimethylaniline radical cation by electron transfer, generating the neutral amine and acetoxyl radical. Transport properties of reactants and solvent polarity changes were investigated and confirmed not to influence the electrochemical behavior forming the basis for our mechanistic hypothesis. To reconcile our conclusion with earlier results, KIEs were reinvestigated electrochemically and by nanosecond laser flash photolysis. Rather than a primary isotope effect (associated with C-H bond cleavage), we believe the observed KIEs are secondary, and can be rationalized on the basis of a quantum effect due to hyperconjugative stabilization in aromatic radical cations during an electron transfer reaction. Product studies performed by constant potential coulometry indicate N,N-dimethylaniline radical cations are catalytic in carboxylate oxidations. Collectively, our results suggest that aminyl radical cation deprotonations may not be as facile as was previously thought, and that in some cases, may not occur at all. Interest in design and synthesis of selenium containing heterocycles stems from their ability to function as antioxidants, anti-virals, anti-inflammatories, and immunomodulators. To establish synthetic feasibility of intramolecular homolytic substitution at selenium for preparation of selenocycles, we set out to determine what factors influence cyclization kinetics. A series of photochemically labile Barton and Kim esters have been syntheisized and employed as radical precursors. The effect of leaving radical stability on kinetics has been investigated through determination of rate constants and activation parameters for intramolecular homolytic substitution of the corresponding radicals via competition experiments. Notable leaving group effects on measured kinetic parameters show more facile reactions for radical precursors with more stable leaving radicals. Moreover, cyclizations to form six-membered (as opposed to five- membered) ring systems exhibited order of magnitude decreases in rate constants for a given leaving radical. Our results are congruent with expectations for radical cyclizations trends for the varied experimental parameters and suggest homolytic substitution affords a convenient means for synthesis of selenocycles. / Ph. D.

Master of Science / Department of Physics / Bharat Ratra / In this report, we discuss the four standard energy conditions of General Relativity (null, weak, dominant, and strong) and investigate their cosmological consequences. We note that these energy conditions can be compatible with cosmic acceleration provided that a repulsive cosmological constant exists and the acceleration stays within certain bounds. Scalar fields and dark energy, and their relationships to the energy conditions, are also discussed. Special attention is paid to the 1988 Ratra-Peebles scalar field model, which is notable in that it provides a physical self-consistent framework for the phenomenology of dark energy. Appendix B, which is part of joint-research with Anatoly Pavlov, Khaled Saaidi, and Bharat Ratra, reports on the existence of the Ratra-Peebles scalar field tracker solution in a curvature-dominated universe, and discusses the problem of investigating the evolution of long-wavelength inhomogeneities in this solution while taking into account the gravitational back-reaction (in the linear perturbative approximation).

Road vehicles are still largely a consumer product and as such the styling of a vehicle becomes a significant factor in how commercially successful a vehicle will become. The influence of styling combined with the numerous other factors to consider in a vehicle development programme means that the optimum aerodynamic package is not possible in real world applications. Aerodynamicists are continually looking for more discrete and innovative ways to reduce the drag of a vehicle. The current thesis adds to this work by investigating the influence of active flow control devices on the aerodynamic drag of square back style road vehicles. A number of different types of flow control are reviewed and the performance of synthetic jets and pulsed jets are investigated on a simple 2D cylinder flow case experimentally. A simplified ¼ scale vehicle model is equipped with active flow control actuators and their effects on the body drag investigated. The influence of the global wake size and the smaller scale in-wake structures on vehicle drag is investigated and discussed. Modification of a large vortex structure in the lower half of the wake is found to be a dominant mechanism by which model base pressure can be influenced. The total gains in power available are calculated and the potential for incorporating active flow control devices in current road vehicles is reviewed. Due to practicality limitations the active flow control devices are currently ruled out for implementation on a road vehicle. The knowledge gained about the vehicle model wake flow topology is later used to create drag reductions using a simple and discrete passive device. The passive modifications act to support claims made about the influence of in wake structures on the global base pressures and vehicle drag. The devices are also tested at full scale where modifications to the vehicle body forces were also observed.

<p>This thesis shows how certain classes of binary constant weight codes can be represented geometrically using linear structures in Euclidean space. The geometric treatment is concerned mostly with codes with minimum distance 2(w - 1), that is, where any two codewords coincide in at most one entry; an algebraic generalization of parts of the theory also applies to some codes without this property. The presented theorems lead to a total of 18 improvements of the table of lower bounds on A(n,d,w) maintained by E. M. Rains and N. J. A. Sloane. Additional improvements have been made by finding new lexicographic codes.</p>

According to recent surveys on the use of systems development methodologies, many organizations claim that they are adapting systems development methodologies (Hardy et al. 1995; Russo et al. 1996; Fitzgerald, 1998). The purpose of this dissertation is to investigate the adaptation of systems development methodologies in South Africa. This problem was investigated by addressing the following research questions: • What are the perceptions of system developers regarding systems development methodologies? • Why do system developers adapt system development methodologies? • How do they adapt the methodologies? • Is there a difference in the quality of the systems which are developed with these adapted systems development methodologies opposed to those systems which are developed according to a specific formalised methodology? In this dissertation, interpretive case studies have been used to add to the researcher's knowledge concerning how and why systems development methodologies in South Africa are adapted. Qualitative interviewing was used as a data collection method. All interviews were recorded and transcribed. The next step was to analyse the transcribed data. In this study, content analysis with cross-case analysis was used. The findings obtained were confirmed by making use of triangulation and member checking. The results indicated that although the use of systems development methodologies is mandatory in organizations, it is not enforced by senior employees. Organizations use multiple systems development methodologies. Systems development methodologies are adapted due to several reasons, i.e. financial gains that is obtained, the lack of knowledge, time limitations, the fact that methodologies are not universally applicable, etc. Systems development methodologies are statically and dynamically adapted by adding and removing steps. The combination of methodologies and switching between methodologies also occur. The results indicate that developers realize that formal systems development methodologies produce systems of a higher quality. However, because it is so time-consuming, they are prepared to accept a lower quality system in order to gain a faster delivery time. / Thesis (M.Sc. (Computer Science))--North-West University, Potchefstroom Campus, 2007.

This thesis presents an investigation of fluorescence resonance energy transfer (FRET) as a reporting signal for protein-protein interactions. Quantitative optical assays to measure protein binding, conjugation and deconjugation are developed and results validated by conventional biochemical techniques. The optical techniques developed provide fast, cheap, quantitative and accurate alternatives to conventional methods. Fluorescent protein fluorophores ECFP and Venus-EYFP were chosen as they are a non-interfering FRET pair and provide an inexpensive and convenient cloning-based labelling method. The small ubiquitin-like modifier SUMO and the SUMOylation pathway leading to its conjugation to target proteins is investigated as a model system. These assays are hence particularly relevant to research on post-translational modification and ubiquitin systems. In protein-protein binding assays we utilise both steady-state and time-resolved FRET detection to measure the equilibrium binding constant of the well-characterised pair SUMO1 and Ubc9. An assay in multi-well plate format is also presented, which uniquely enables repeat measurements under varying conditions and under the addition of further substances. The multi-protein binding interactions of the SUMOylation pathway including RanBP2 are analysed in binding inhibition assays. Our results clarify the role of RanBP2: a covalent SUMO1-Ubc9 link is required for the formation of a trimeric complex, although mutual binding sites are present on all three proteins. Furthermore, the binding of SUMO1 and Ubc9 is disrupted by RanBP2, which may be an essential step in transferring SUMO1 to its target protein. A FRET-based kinetic study of this conjugation process to RanGAP1 is presented. An assay to monitor the deconjugation of SUMO1 by specific proteases is established using a doubly-tagged SUMO construct. This enables a quantitative analysis of protease and substrate specificity based on real-time kinetic data, a characterisation of crude cell extracts and a high-throughput screen for protease inhibitors using FRET. A screen of the National Cancer Institute (NIC) diversity set for SenP1 inhibition reveals nine suitable compounds, which are potential anti-cancer drugs. The results of two further projects, the study of protein-protein binding by measuring small refractive index changes and the autofluorescence of normal and neoplastic cervical tissue models are also presented. In the latter, principal component analysis was used to systematically identify emission regions of significant variation between samples, enabling discrimination between healthy and pre-cancerous tissue models.

Methylmercury (MeHg) is a neurotoxic compound that threatens the well-being of humans and wildlife. It is formed through the methylation of inorganic mercury (HgII) under suboxic/anoxic conditions in soils, sediment and waters. The chemical speciation of HgII, including specific HgII species in aqueous and solid/adsorbed phases, plays a key role in MeHg formation. Chemical forms of HgII which have been reported to be available for uptake in methylating bacteria include neutral HgII–sulfide complexes, HgII complexes with specific low molecular mass (LMM) thiols, and nanoparticulate HgS(s). Accurate determination of the chemical speciation of HgII is thus crucial when elucidating the mechanism of MeHg formation. The concentration of HgII–LMM thiols complexes is predicted to be extremely low (sub fM range). Current analytical methods do not allow direct quantification of HgII complexes due to the very low concentration of these complexes, and therefore determination rely on thermodynamic modeling. Accurate stability constants for HgII–LMM thiols complexes and quantification of LMM thiol ligands in environments are thus required to precisely determine the concentration of such complexes. In this thesis, a novel analytical method was developed based on online pre-concentration coupled with liquid chromatography tandem mass spectrometry to determine the concentration of 16 LMM thiols (Paper I). This method was successful in detecting 8 LMM thiols in boreal wetland porewaters, with mercaptoacetic acid and cysteine being the most abundant. The total concentration of individual detected LMM thiols ranged from sub nM (LOD=0.1 nM) to 77 nM. Moreover, the stability constant (β2) for HgII complexes with 15 LMM thiols were directly determined for the first time by competing ligand exchange experiments combined with liquid chromatography ICPMS analysis (Paper II). Values of log β2 for the reaction Hg2+ + 2LMM-RS- = Hg(LMM-RS)2 ranged from 34.6 for. Based on the determined constants of Hg(LMM-RS)2 complexes and state-of-the-art constants from literature for other HgII complexes, we established comprehensive thermodynamic speciation models for MeHg and HgII in boreal wetlands (Paper III). The speciation of HgII was coupled with the HgII methylation rate constant (km) determined with different enriched Hg isotope tracers (Paper IV). There was a good correlation (R2=0.88) between the km determined by a HgII(aq) tracer added as Hg(NO3)2 with high bioavailability and a tracer where HgII was bond to thiol groups in natural organic matter (HgII-NOM(ads)) and has a lower bioavailability. The HgII(aq) tracer was consistently methylated at 5 times higher rate than the HgII-NOM(ads) tracer. A good correlation was observed between the concentration of biologically produced LMM thiols and km in the boreal wetlands. In a mesocosm study of estuarine sediment-brackish water systems, increased concentration of phytoplankton chlorophyll α due to macro nutrient additions led to an increase in HgII methylation rate of the HgII(aq) but not of the HgII-NOM(ads) tracer or ambient HgII species (Paper V). Furthermore, simulated newly deposited HgII species from atmospheric and terrestrial sources were exhibited significantly higher HgII methylation rates when compared with simulated aged sediment HgII pools. Through the development and adoption of novel analytical methods, this thesis reveals the significance of LMM thiols in Hg biogeochemistry by precise determination of HgII–LMM thiol complexes in natural environmental systems.