The purpose of this thesis was to investigate age related variations in anterior ocular characteristics and their effect on the response to short-term (one-hour) open eye orthokeratology (OK) lens wear and overnight hydrogel lens wear. Sixty-three volunteer subjects were divided into three groups comprising children (Group I), young adults (Group II) and older adults (Group III). Anterior ocular characteristics that were measured included corneal topography with the Medmont corneal topographer, total, stromal and epithelial thickness with the Holden-Payor optical pachometer, microscopic cell characteristics with the confocal microscope, corneal aesthesiometry, corneal modulus of elasticity, and tear film, palpebral and eyelid characteristics. The older cornea was found to be more spherical in shape (asphericity Q = -0.24 ?? 0.07 in group III compared to -0.34 ?? 0.09 and -0.32 ?? 0.17 in groups I and II respectively) and showed a tendency from with-the-rule to against-the-rule astigmatism. Older subjects also had a decreased endothelial cell density (2596 ?? 111 cells/mm2 in group III compared to 2793 ?? 138 cells/mm2 in group II) and reduced eyelid tension compared to the younger groups. Children were found to have lower blink rates than adults. It was concluded that these changes might have a significant impact on contact lens wear. The responses to two contact lens based techniques, orthokeratology (OK) and hydrogel overnight wear (ON), were studied. Sixty subjects (20 per group) wore reverse-geometry lenses (BE, Ultravison Aust Pty Ltd) of Boston XO material in one eye under open eye conditions for one hour in one eye only. Changes in unaided visual acuity, corneal curvature, corneal thickness and confocal microscopic variables were measured after OK lens wear. All subject groups showed statistically significant improvements (p &lt 0.05) in unaided visual acuity, a trend for more positive (less prolate) corneal asphericity, increase in apical corneal radius and decrease in central total corneal thickness, after OK lens wear. When the groups were stratified, statistical significance was obtained between the older group and the other two younger groups for change in apical corneal radius (0.23 ?? 0.01 mm in group I, 0.15 ?? 0.01 mm for group II, 0.06 ?? 0.33 for group III). The change in asphericity also showed significant differences between the older age group (0.10 ?? 0.08 mm) and the young adult group (0.21 ?? 0.13). Central corneal thinning obtained after one hour of OK lens wear revealed significant differences between the older adult group (-1.9 ?? 3.2 ??m) and the other two groups (-5.0 ?? 5.9 ??m in group I, -5.0 ?? 2.0 ??m for group II). Sixty subjects wore hydrogel lenses (One-Day Acuvue, Johnson and Johnson Visioncare Pty Ltd) made of etafilcon A in one eye only overnight during sleep at their home. Changes in unaided visual acuity, corneal curvature, corneal thickness, confocal microscopic variables and slitlamp variables were measured after ON lens wear. All subjects showed significant thickening (p &lt 0.05) of the cornea with ON wear, more in the lens-wearing eye. This study reported that the edema response induced by Acuvue contact lenses is stromal in origin. The edema response of the older group (33.9 ?? 11.5 ??m) was lower than the other two groups as demonstrated by the difference in the change in central stromal thickness (49.3 ?? 20.3 ??m for group I and 51.0 ?? 20.0 ??m for group II). However, a statistically significant difference in the change in thickness was not reached either for the central total corneal thickness or for other topographic locations. Corneal and visual changes found in this study confirm previous reports of the effects of short term OK and hydrogel ON lens wear. Older lens wearers showed a reduced or delayed response to short-term lens wear. Studies investigating the effects of age with long lens-wearing durations are warranted to quantify these effects further.

There is considerable evidence to indicate that most gas permeable contact lenses do not transmit sufficient oxygen to supply all the corneal oxygen requirement. This problem is further exacerbated by non-valid methods of characterizing the oxygen performance of such lenses. The current methods of using oxygen permeability (Dk) and oxygen transmissibility (Dk/L) as indices of oxygen performance of contact lenses is completely erroneous. Dk and Dk/L pertain to contact lens materials in flat sheet form having uniform thickness and equal diffusion path at all points on the surface. Finished contact lenses, of necessity, are curved surfaces and of varying thickness. Consequently the concept of Dk and Dk/L cannot be applied to contact lenses. To date there are no studies to determine the absolute oxygen tension under gas permeable contact lenses on the human eye. All attempts to quantify the oxygen tension under a lens have been by indirect methods or by predicting the p02 from Dk values, using mathematical equations. These results do not match the clinical findings. This study was done to show that oxygen flux through a contact lens, measured in vitro, is a better determinant of the in vivo oxygen performance of gas permeable contact lenses. A special cell was designed to measure the oxygen flux, in vitro under standardised conditions. Contact lens microelectrodes were designed to measure the oxygen tension in vivo. The data obtainedwas used to develop a model for the oxygen performance of rigid gas permeable lenses on the human eye. / Thesis (M.Optom.)-University of Durban-Westville, 1989.

The purpose of this thesis was to investigate age related variations in anterior ocular characteristics and their effect on the response to short-term (one-hour) open eye orthokeratology (OK) lens wear and overnight hydrogel lens wear. Sixty-three volunteer subjects were divided into three groups comprising children (Group I), young adults (Group II) and older adults (Group III). Anterior ocular characteristics that were measured included corneal topography with the Medmont corneal topographer, total, stromal and epithelial thickness with the Holden-Payor optical pachometer, microscopic cell characteristics with the confocal microscope, corneal aesthesiometry, corneal modulus of elasticity, and tear film, palpebral and eyelid characteristics. The older cornea was found to be more spherical in shape (asphericity Q = -0.24 ?? 0.07 in group III compared to -0.34 ?? 0.09 and -0.32 ?? 0.17 in groups I and II respectively) and showed a tendency from with-the-rule to against-the-rule astigmatism. Older subjects also had a decreased endothelial cell density (2596 ?? 111 cells/mm2 in group III compared to 2793 ?? 138 cells/mm2 in group II) and reduced eyelid tension compared to the younger groups. Children were found to have lower blink rates than adults. It was concluded that these changes might have a significant impact on contact lens wear. The responses to two contact lens based techniques, orthokeratology (OK) and hydrogel overnight wear (ON), were studied. Sixty subjects (20 per group) wore reverse-geometry lenses (BE, Ultravison Aust Pty Ltd) of Boston XO material in one eye under open eye conditions for one hour in one eye only. Changes in unaided visual acuity, corneal curvature, corneal thickness and confocal microscopic variables were measured after OK lens wear. All subject groups showed statistically significant improvements (p &lt 0.05) in unaided visual acuity, a trend for more positive (less prolate) corneal asphericity, increase in apical corneal radius and decrease in central total corneal thickness, after OK lens wear. When the groups were stratified, statistical significance was obtained between the older group and the other two younger groups for change in apical corneal radius (0.23 ?? 0.01 mm in group I, 0.15 ?? 0.01 mm for group II, 0.06 ?? 0.33 for group III). The change in asphericity also showed significant differences between the older age group (0.10 ?? 0.08 mm) and the young adult group (0.21 ?? 0.13). Central corneal thinning obtained after one hour of OK lens wear revealed significant differences between the older adult group (-1.9 ?? 3.2 ??m) and the other two groups (-5.0 ?? 5.9 ??m in group I, -5.0 ?? 2.0 ??m for group II). Sixty subjects wore hydrogel lenses (One-Day Acuvue, Johnson and Johnson Visioncare Pty Ltd) made of etafilcon A in one eye only overnight during sleep at their home. Changes in unaided visual acuity, corneal curvature, corneal thickness, confocal microscopic variables and slitlamp variables were measured after ON lens wear. All subjects showed significant thickening (p &lt 0.05) of the cornea with ON wear, more in the lens-wearing eye. This study reported that the edema response induced by Acuvue contact lenses is stromal in origin. The edema response of the older group (33.9 ?? 11.5 ??m) was lower than the other two groups as demonstrated by the difference in the change in central stromal thickness (49.3 ?? 20.3 ??m for group I and 51.0 ?? 20.0 ??m for group II). However, a statistically significant difference in the change in thickness was not reached either for the central total corneal thickness or for other topographic locations. Corneal and visual changes found in this study confirm previous reports of the effects of short term OK and hydrogel ON lens wear. Older lens wearers showed a reduced or delayed response to short-term lens wear. Studies investigating the effects of age with long lens-wearing durations are warranted to quantify these effects further.

Thesis (Ph. D.)--Ohio State University, 2003. / Title from first page of PDF file. Document formatted into pages; contains xv, 144 p.; also includes graphics (some col.) Includes bibliographical references (p. 129-144). Available online via OhioLINK's ETD Center

Contact lens manufacture requires a high level of accuracy and surface integrity in the range of a few nanometres. Amidst numerous optical manufacturing techniques, single-point diamond turning is widely employed in the making of contact lenses due to its capability of producing optical surfaces of complex shapes and nanometric accuracy. For process optimisation, it is ideal to assess the effects of various conditions and also establish their relationships with the surface finish. Presently, there is little information available on the performance of single point diamond turning when machining contact lens polymers. Therefore, the research work undertaken herewith is aimed at testing known facts in contact lens diamond turning and investigating the performance of ultra-high precision manufacturing of contact lens polymers. Experimental tests were conducted on Roflufocon E, which is a commercially available contact lens polymer and on Precitech Nanoform Ultra-grind 250 precision machining. Tests were performed at varying cutting feeds, speed and depth of cut. Initial experimental tests investigated the influence of process factors affecting surface finish in the UHPM of lenses. The acquired data were statistically analysed using Response Surface Method (RSM) to create a model of the process. Subsequently, a model which uses Runge-Kutta’s fourth order non-linear finite series scheme was developed and adapted to deduce the force occurring at the tool tip. These forces were also statistically analysed and modelled to also predict the effects process factors have on cutting force. Further experimental tests were aimed at establishing the presence of the triboelectric wear phenomena occurring during polymer machining and identifying the most influential process factors. Results indicate that feed rate is a significant factor in the generation of high optical surface quality. In addition, the depth of cut was identified as a significant factor in the generation of low surface roughness in lenses. The influence some of these process factors had was notably linked to triboelectric effects. This tribological effect was generated from the continuous rubbing action of magnetised chips on the cutting tool. This further stresses the presence of high static charging during cutting. Moderately humid cutting conditions presented an adequate means for static charge control and displayed improved surface finishes. In all experimental tests, the feed rate was identified as the most significant factor within the range of cutting parameters employed. Hence, the results validated the fact that feed rate had a high influence in polymer machining. The work also established the relationship on how surface roughness of an optical lens responded to monitoring signals and parameters such as force, feed, speed and depth of cut during machining and it generated models for prediction of surface finishes and appropriate selection of parameters. Furthermore, the study provides a molecular simulation analysis for validating observed conditions occurring at the nanometric scale in polymer machining. This is novel in molecular polymer modelling. The outcome of this research has contributed significantly to the body of knowledge and has provided basic information in the area of precision manufacturing of optical components of high surface integrity such as contact lenses. The application of the research findings presented here cuts across various fields such as medicine, semi-conductors, aerospace, defence, telecom, lasers, instrumentation and life sciences.

The cornea is an avascular transparent tissue exposed to the environment and therefore highly susceptible to damage. With an increase in corneal refractive surgeries, corneal transplants, and corneal injuries, understanding and improving corneal healing mechanisms are extremely important. Impaired healing of corneal wounds can lead to decreased visual acuity and extreme pain. Serum eye drops, amniotic membranes, pharmaceutical agents, biopolymers, and cell transplants are just a few approaches that have been employed to improve wound healing. Bandage contact lenses (BCLs) have been proposed as a simple method to facilitate wound healing while reducing pain. The synthesis of a silicone hydrogel contact lens capable of surface binding hyaluronic acid (HA) for corneal wound healing was explored in the current work. HA was used as both a wetting agent and a therapeutic. The work presented describes the synthesis, characterization, and cell testing of the HA binding model silicone hydrogels, composed of the hydrophilic monomer, 2-hydroxyethyl methacrylate (HEMA) and a hydrophobic silicone monomer, methacryloxypropyltris (trimethylsiloxy) silane (TRIS). Three different methods were evaluated for increasing HA binding and improving surface wettability. “Caged lenses” utilized the same base polymer with the incorporation of methacrylated N-Hydroxysuccinimide (NHS). Hydrophilic polyethylene glycol (PEG) chains were then tethered from the NHS, forming a “cage” with the potential to physically entrap HA. Although surface wettability was improved, less HA was entrapped in the caged lenses compared to model silicone hydrogels, presumably due to the increased hinderance resulting from the PEG chains. “Tethered HA” lenses utilized PEG as a spacer to conjugate HA to the lens surface in order to improve surface hydrophilicity. Methacrylated HA conjugation resulted in a significant decrease in contact angle (p <0.01) compared to model pHEMA-co-TRIS whereas tethered thiolated HA did not lead to a significant decrease (p >0.05) in contact angle. It was clear that neither of these methods would lead to sufficient HA binding. Ionic interaction lenses utilize monomers and small molecules that contain a positive charge to bind to the negatively charged HA under physiological conditions. The monomer diethylaminoethyl methacrylate (DEAEM) was polymerized directly into the polymer backbone, but resulted in no significant decrease (p >0.05) in contact angle. In comparison, surface functionalization using thiolene “click” chemistry allowed conjugation of the small molecule, dimethylamino ethanethiol (DMAET) and diethylamino ethanethiol (DEAET). DMAET and DEAET modified lenses showed significantly higher (p <0.001) HA binding compared to model pHEMA-co-TRIS controls at all time points. The modified lenses improved release kinetics preventing an initial burst release and showed consistent release when unloaded and reloaded with HA. The contact angle was significantly decreased (p <0.05) for the modified lenses with HA without affecting the equilibrium water content. Optical transparency was reduced following lens modifications although the thickness of the disks prepared was higher than a typical contact lens. Finally, the modified lenses did not exhibit any cytotoxicity in vitro with human corneal epithelial cells (HCECs). The synthesis of silicone hydrogels capable of surface binding HA have potential to be used as a bandage contact lens while improving surface wettability and enhancing comfort. / Thesis / Master of Applied Science (MASc)

No description available.

Silicone hydrogels haves been widely utilized in many in ophthalmic and other biomedical applications due to the its comfort of hydrogels, their excellent biocompatibility, high oxygen permeability and transparency. For use as a contact lens, the silicone hydrogel with interacts with the tear film, cornea, and eyelid;, thus surface properties of the gel are crucial to be considered. The highly oxygen permeabilitye performance of the silicone hydrogel contact lens materials mainly relies on the incorporationng of the siloxane functional groups., Hhowever these groups are extremely mobile and surface active, which can result in an increase in the of lens surface hydrophobicity, as well as protein and lipid deposition. Therefore, there is a need for surface modification of silicone hydrogel contact lenses. Otherwise users may might have to choose to decrease the frequency and length of wearing duration of silicone contact lenses due to dryness or bio-fouling related issues. A novel biomimetic methacrylate monomer which contains a phosphorylcholine group, 2-mMethacryloyloxyethyl pPhosphorycholine (MPC) is was grafted onto the surface of novel silicone hydrogel materials surface to create a thick hydration layer in order to enhance the protein resistance and surface wettability. Low temperature air plasma has beenwas chosen to initiate grafting polymerization of MPC monomers onto silicone hydrogel substrates. Hydrogels were treated with plasma and exposed to air flow to yield hydroperoxides on the surface; the, and peroxides group acted as a photo-initiators for further thermal MPC grafting polymerization. After surface modification, the silicone hydrogels were characterized by XPS and ATR-FTIR to confirm the structure and elemental composition. A significant amount of phosphorus element was found shown on the XPS spectra of the modified materialsum,, demonstrating that so the MPC monomers were successfully grafted onto the gel surface. According to water contact measurement results, the modified samples possessed very hydrophilic surfaces, with advancing angles of about 27°, while compared the unmodified samples at around 110°. After surface grafting, between a around 20% and to 50%’s reduction in protein deposition was also observed, which aligned with water contact angle results. Other properties such as oxygen permeability, transparency, water equilibrium, and elastic modulus remained unchanged after the air plasma exposure and thermal MPC polymerization. / Thesis / Master of Applied Science (MASc)

No description available.

Contact lens success is primarily driven by comfort of the lens in eye. Over the years, many modifications have been made to the lens surface and bulk material to improve comfort of the lens, however 50% of contact lens wearers still report dry eye symptoms while wearing their lenses. Wettability of the lens material plays a large role in lens comfort, primarily due to its influence in tear film stability. In vitro wettability of contact lenses has typically been assessed by measuring the water contact angle on the lens surface. Currently there are three techniques to measure the in vitro wettability of contact lenses, the sessile drop technique, captive bubble technique, and the Wilhelmy balance method. To date, there is much published on assessing wettability using the sessile drop and captive bubble technique, however there is no data published looking at the in vitro wettability of hydrogel contact lenses measured by the Wilhelmy balance method. Accumulation and deposition of tear components on the lens surface can also affect lens performance, by altering the wettability of the lens surface and causing lens spoilage. The majority of in vitro studies looking at deposition of tear components on the lens surface dope the lenses in tear solutions for a set period of time. None of these studies have investigated the impact of exposing the lenses to tear solutions, then exposing them to the air and then back into the tear solution, which mimics the process during blinking. In Chapter 2, an evaluation of the influence of lens preparation on the wettability of contact lenses measured by the sessile drop technique was conducted. The wettability of 6 silicone hydrogel and one conventional lens material was assessed. Lenses were blot dried on either a microfiber cloth or lens paper for different drying periods and contact angles were measured using the sessile drop technique. There were large variations in results using the microfiber cloth after all drying periods, but there was little variation in results after lenses were blot dried on lens paper for approximately 20 seconds. Thus, it was determined that for future contact angle analysis using the sessile drop technique that lenses should be blot dried for roughly 20 seconds on lens paper. This method was used consistently for the rest of the experiments in which the sessile drop technique was used to measure contact angles. The remainder of Chapter 2 compared the contact angles of different lens materials measured by the sessile drop technique and Wilhelmy balance method. The wettability of five different silicone hydrogel lens materials was assessed directly out-of-blister and after a 48 hour soak in saline. There were significant differences in contact angles for the lens materials between the two techniques. There were also significant differences in contact angles directly out-of-blister and after the 48 hour soak. Results from this study suggested that different methods of measuring wettability can produce different results and that blister pack solutions can alter the wettability of lens materials. Chapter 3 measured the physical properties of blister pack solutions of silicone hydrogel lenses. The pH, osmolality, surface tension, and viscosity of the blister solutions for 9 silicone hydrogel lenses, 2 conventional lenses, and 2 saline solutions were measured. The osmolality of the blister solutions followed a trend, in that blister solutions manufactured by the same company had the same osmolality. Products produced by Johnson & Johnson had the highest osmolality. Blister solutions that contained additional wetting agents had higher viscosities compared to blister solutions without added wetting agents. The main conclusion from this study was that adding wetting agents to blister solutions could alter the physical properties of the blister solutions. The purpose of Chapter 4 was to measure the physical properties of the blister pack solutions of daily disposable lenses and to evaluate the wettability of the lens materials and substantivity of the blister solutions, using a method in which lenses were cycled through 5 minute soaks in saline to mimic blinking. Five daily disposable lens materials were evaluated, one of which was shipped in a blister solution with added surfactants and wetting agents. The wettability of the lenses was assessed using the sessile drop technique and Wilhelmy balance method. The lens with the modified blister solution had a lower surface tension and higher viscosity compared to all the other blister solutions. The same trend in osmolalties as those reported in Chapter 3, were found with blister solutions made by the same manufacturer having the same osmolality. The wettability varied across lens materials. Overall, the lens material with the added components to the blister solution had the lowest contact angle. Chapter 5 investigated the deposition of tear components onto the surface of conventional and silicone hydrogel lens materials and looked at the impact of this on changes in wettability. Three lens materials used in Chapter 4 were exposed to a saline solution, lysozyme solution, and a complex tear solution for 5 minutes, 1 hour, 4 hours, and 8 hours. The wettability was assessed after each time point using the sessile drop and Wilhelmy balance methods. There was little to no deposition on the lens materials that had the highest in vitro CAs in Chapter 4, exemplified by no change in wettability after being soaked in the lysozyme and complex tear solutions. There was deposition on the lens materials with the lowest CAs in Chapter 4, exemplified by a significant increase in wettability after being soaked in the lysozyme and complex tear solutions. Results indicate that there is some deposition onto one lens material, as shown by the change in wettability of the lens surface. These results were further used to validate a method used in Chapter 6. The experiment conducted in Chapter 6 was similar to the experiment in Chapter 5, except that the lenses were not soaked in the three solutions but rather exposed to the solutions in a “model blink cell”. The model blink cell moves lenses in and out of solution at a set time interval, in an attempt to mimic blinking. The interval was set so the lenses would be placed for 1 second in solution and 5 seconds exposed to the air. The same lens materials used in Chapter 5 were used in for this experiment. The lenses were exposed to a saline solution, lysozyme solution and complex tear solution for 5 minutes, 1 hour, 4 hours, and 8 hours. Much like in Chapter 5, deposition on the lens materials was determined by a change in the lens wettability. There were differences in the results of this chapter and that of Chapter 5, with deposition occurring on two of the lens materials rather than just one. This result indicates that the drying of the lens surface for 5 seconds out of solution has an effect on the deposition of tear components on certain lens materials. Thus, the model blink cell may be a useful tool for future deposition studies. Overall this thesis demonstrated that preparation of the lens material can cause variation in contact angles. Different methods of measuring in vitro wettability of contact lenses can produce different results and thus the method used to assess wettability should always be stated. The physical properties of blister pack solutions can change with added wetting agents and surfactants, and components from blister solutions can alter the initial wettability of contact lenses. In vitro deposition of proteins onto the lens surface can vary with techniques, and finally, deposition of tear components onto the surface of contact lenses can alter the lens wettability.