<p>High density sampling (n=50) was conducted in Cootes Paradise, a shallow wetland on the western shoreline of Lake Ontario near the city of Hamilton. Cootes Paradise is an urban wetland that has been affected by pollutants and nutrients and invasive carp. Thecamoebian analyses paired with site specific environmental measurements (depth, sp. conductivity, temperature, DO and pH) and substrate characteristics (textural and organic content -LOI) show relationships (R² = 0.6) with depth (0-1m) and corresponding sp. conductivity (0.5 to 0.65 mS/cm) and temperature (26.5 to 30.5 °C). Q-mode cluster analysis recognized two biofacies. Biofacies 1 samples (n= 26) are found in the deeper areas (0.70 ± 0.27 m) and dominated by <em>C. tricuspis</em> 36 ± 8% (1 std), <em>L. vas</em> 18 ± 13% and <em>D. protaeiformis “claviformis”</em> 14 ± 6%. Mean water temperature is 28.0 ± 0.6 °C and conductivity at 0.56 ± 0.04 mS/cm. This assemblage has low species diversity (SDI=1.9 ± 0.3) which indicates a transitional environment. Biofacies 2 contains samples (n= 24) which are found in shallower areas (0.38 ± 0.15 m) and the assemblage is characterized by <em>C. constricta “aerophila”</em> 25 ± 8%, <em>C. tricuspis</em> 18 ± 5%, <em>Cyclopyxis sp.</em> 9 ± 6 % and <em>L. vas</em> 9 ± 4 %. The SDI for Biofacies 2 is 2.2 ± 0.2 and like Biofacies 1 shows a transitional environment. The average temperature is and 29.0 ± 1.0 °C with mean sp. conductivity also slightly higher than Biofacies 1 at 0.6 ± 0.04 mS/cm.</p> / Master of Science (MSc)