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Copper radionuclides possess physical properties suitable for utilization in nuclear medicine. Important part of their preparation is separation from mother elements, usually zinc or nickel. Currently used methods of separation have their limits, what opens field for finding new ways. One part of the Thesis contains preparation of simple ion exchangers and their selectivity study. The other part of the Thesis is dedicated to optimization of synthetic steps leading to new bifunctional ligands in suitable form for binding to macromolecular carriers and preparation of new set of exchangers.