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NaCl-induced and -repressed cDNA clones had previously been isolated by differential screening of a cDNA library, prepared from poly(A(^+)) RNA isolated from Distichlis spicata (salt grass) cell cultures grown in the presence of 260 mM NaCl (Zhao, et al., 1989). Eight of these cDNA clones have now been subcloned and/or sequenced and the predicted polypeptides compared with owl sequence data base. Three clones pDZ6.2, pDZVIII 1.2.1 and pDZIX 3.1 encode proline rich proteins, containing an amino acid repeat [PPKKDH(H)Y(Y)]. They have similar amino acid usage to proline-rich cell wall proteins, being rich in P, K, H and Y. The first 20 amino acid residues encode a putative leader sequence, supporting the proposed extracellular role as a cell wall protein. This N-terminal sequence (MPLLVALLLVLAVVAAAGAD) shares some similarity with die leader sequence of a soyabean proline-rich cell wall protein precursor and other extracellular proteins (the conserved residues are underlined). There is an increase in abundance of transcripts hybridising to the inserts from pDZ6.2 and pDZVUI 1.2.1 in response to either 520 mM NaCl or 100 µM ABA, but a decrease in response to 5 mM exogenous proline. It is suggested that the corresponding gene(s) are regulated at the level of either transcription or transcript stability, in response to elevated NaCl, with ABA as a mediator of (or part of) tills response. pDZ6.2 and pDZXI 3.1 have identical nucleotide sequences, whilst pDZVni 1.2.1 differs in three base paks within the putative open reading frame, suggesting that there may be at least two members of a multi gene family. A 68 bp OA repeat has been found in the 5' untranslated region of pDZ6.2 and a corresponding transcript identified by northern analysis using this OA sequence as a probe. Such nucleotide repeats can form triplexes (DNA) or hakpin loops (RNA), which is dependent on pH and ionic conditions. Therefore this OA repeat may play a role in the regulation of the gene corresponding to pDZ6.2 at the level of transcription or translation, possibly by attenuation of these processes, either by the formation of triplexes or hah-pins, or the binding of a protein to this GA region, at low ionic strength. However initial in vitro ttanscription experiments, to compare the transcriptional activity of pDZ6.2 and pDZVin 5.1.1 at different ionic strengths, proved inconclusive. An attempt was also made to identify the corresponding genomic region from D. spicata by anchored PGR.A fourth clone pDZ2.8L encodes a histone 2B protein, having 97.9% similarity to a wheat histone 2B. Its transcript abundance decreased in response to either 520 mM NaCl, 5 mM proline or 100 µM ABA. The sequences of the remaining clones either revealed no significant similarity to any known sequences or were assigned as being cloning artefacts .D. spicata cells accumulate proline within eight hours of exposure to 260 mM NaCl (Heyser, et al., 1989b). An unsuccessful attempt was also made to isolate a pyrroline-5- carboxylate reductase gene homologue from D. spicata, by heterologous probing of Southern blots with a soyabean cDNA pProCl and PCR.

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In the 1990s social scientists researching public perceptions of GM foods recommended the introduction of new modes of public participation to make decision-making more inclusive. This research utilises categories and concepts from cultural theory (as developed by anthropologists Mary Douglas, Michael Thompson and Steve Rayner, and political scientists Aaron Wildavsky and Richard Ellis) as tools to consider how a range of opinions, illustrated by cultural theory's ideal types, were represented within the GM crop debate. By doing so, it considers whether this approach supports the notion that greater public participation would improve inclusiveness. Furthermore, it considers how best to model the ways that individuals utilised the discourse of cultural theory's ideal types, and whether this has implications for seeking greater inclusiveness. The research is based upon 55 one-to-one interviews that were conducted with various individuals engaged in the GM crop debate in 2000, and secondary sources of data such as media coverage, electronic mailing lists and public meetings between 1996 and 2002. It was determined that the discourses of cultural theory's ideal types were best presented along a continuum, with the hierarchists in the centre and the egalitarians and individualists at either extreme. Within the wider public debate, the media and public reflected the concerns of egalitarians, while government discourse reflected the concerns of hicrarchists. By enabling the public to have a greater say in decision-making,it is asserted that decisions will favour the egalitarian outlook and will ignore the wisdom of cultural theory's other ideal types. Furthermore, since the discourse of those engaged in the debate included all but the most fatalistic comments made by members of the public, it is argued that public participation is unlikely to bring any new voices to the debate. Rather than introducing new modes of public participation,t his research recommends that the government actively consults with individuals already engaged in the GM crop debate on all issues, including those of a more political nature. The aim of such an exercise would be to identify, communicate and consider the full range of opinions available so that decisions can become more fully informed, transparent and trusted.

This research is concerned with exploring differences in the meaning of entrepreneurial behaviour among owner-managers of agricultural enterprises in Bulgaria. It aims to gain a better understanding of the origin and development of small businesses in a postsocialist context. Entrepreneurial behaviour is understood as the start-up and development of a new business and is examined through the views and experiences of agricultural producers. An exploratory conceptual framework, encompassing the individual owner-manger, the enterprise and the family, is developed to guide the investigation. It facilitated an analysis of the entrepreneurial process and the relationship between agricultural producers and their enterprises, while also taking into account the possible influences of involved family members. Data were drawn from qualitative face-to-face interviews with a diverse sample of 83 agricultural producers in four rural settlements. The respondents represented different demographic characteristics and agricultural sub-sectors. The findings from this exploratory research provide useful insights into the entrepreneurial behaviour of Bulgarian agricultural producers. Different patterns of engagement with the agricultural enterprises emerge. These are explained through startup motivations and growth aspirations and achievement, which in turn are related to differences in the scale of enterprises. Furthermore, patterns of engagement are related to differences in orientation towards self-employment and adoption of the role of owner-manager. Start-up motivations are associated with a complex interaction of different factors. They are also strongly related to the post-socialist context as represented by macro-level changes. The interviewees recognised and reacted differently to these changes. Presence of involved family members could have either a positive or negative influence on the start-up of new additional activities or the enlargement of existing ones. Contextual issues like the post-socialist background, culture and family contributed to a better understanding of the businesses and entrepreneurial behaviour. The results suggest that the entrepreneurial behaviour of post-socialist agricultural producers requires further research in order to gain an indepth understanding of why they undertake their own business.