Subtropical deforestation in Latin America is thought to be driven by demand for agricultural land, particularly to grow soybeans. However, existing remote sensing methods that can differentiate crop types to verify this hypothesis require high spatial or spectral resolution data, or extensive ground truth information to develop training sites, none of which are freely available for much of the world. I developed a new method of crop classification based on the phenological signatures of crops extracted from multi-temporal MODIS vegetation indices. I tested and refined this method using the USDA Cropland Data Layer from Kansas, USA as a reference. I then applied the method to classify crop types for a study site in Pellegrini, Santiago Del Estero, Argentina. The results show that this method is unable to effectively separate summer crops in Pellegrini, but can differentiate summer crops and non-summer crops. Unmet assumptions about agricultural practices are primarily responsible for the ineffective summer crop classification, underlining the need for researchers to have a complete understanding of ground conditions when designing a remote sensing analysis.

This study’s main goal was to evaluate Bacillus subtilis inoculation and mine water irrigation effect on germination, growth, nodulation, physiology and shoot/grain quality of cowpea genotypes exposed to extreme climatic conditions (elevated temperatures). The first experiment evaluated the interactive effect of Bacillus subtilis (BD233) inoculation and elevated temperature on germination indices and plumule lengths of three genotypes (Asetanapa, Soronko and Nyira) of cowpea. The results showed that interaction between B. subtilis (BD233) and temperature significantly (p<0.05) influenced the germination indices (germination percentage (G%), germination index (GI) and germination rate index (GRI)) and plumule length of cowpea seedlings and genotype responses were significantly different. At elevated temperature (35oC), inoculation with B. subtilis (BD233) enhanced seed germination and growth of cowpea. The second experiment evaluated the effect of temperature on growth and nutritional content of cowpea incubated for seven days in a growth chamber. The results showed that when cowpea genotype, Soronko, was incubated at different temperature regimes, the whole plant biomass, shoot carbon and crude protein contents were significantly affected with temperature increases at all three stages of the plants’ life cycle. The results suggest that the pre-flowering (40 DAP) and flowering (90 DAP) stages of cowpea compared to post-flowering (123 DAP) are more susceptible to elevated temperatures (30-35oC). The third experiment evaluated Bacillus subtilis inoculation and mine water irrigation effect on growth, nodulation, physiology and nutritional content of cowpea under glasshouse conditions. The results revealed that the interaction of B. subtilis (BD233) inoculation and mine water (75% AMD) irrigation was significant for the growth, nodulation, stomatal conductance, chlorophyll contents and shoot/grain nutritional quality of cowpea genotypes. In comparison with control, generally, B. subtilis inoculation enhanced the growth, nodulation and yield of all tested cowpea genotypes and irrigation with mine water significantly influenced the mineral contents in both shoot and grain of cowpea. Taken together, findings in this study have implications for cultivation of cowpea, an important candidate for food/nutrition security in Africa, under future climate change scenarios. / Environmental Sciences / M. Sc. (Environmental Sciences)

Doctor of Philosophy / Department of Agronomy / P. V. Vara Prasad / Leguminous cover crops systems have been envisaged as a critical component of sustainable agriculture due to their potential to increase soil productivity through cycling of carbon (C) and nitrogen (N) in agricultural systems. The objectives of this study were to evaluate the performance of leguminous summer cover crops; cowpea [Vigna unguiculata (L.) Walp.], pigeon pea [Cajanus cajan (L.) Millsp], sunn hemp (Crotalaria juncea L.) and double-cropped grain crops; grain sorghum [Sorghum bicolor (L.) Moench] and soybean [Glycine max (L.) Merr.] after winter wheat (Triticum aestivum L.) and to determine the effects of these crops and varying N rates in the cropping system on nitrous oxide (N[subscript]2O) emissions, growth and yield of succeeding grain sorghum and maize (Zea mays L.) crop, soil aggregation, aggregate-associated C, and N. Field and laboratory studies were conducted for two years. The cover crops and double-cropped grain crops were planted immediately after winter wheat harvest. The cover crops were terminated at the beginning of flowering. Nitrogen fertilizer (urea 46% N) rates of 0, 45, 90, 135, and 180 kg N ha[superscript]-1 were applied to grain sorghum or maize in fallow plots. Pigeon pea and grain sorghum had more C accumulation than cowpea, sunn hemp and double-cropped soybean. Pigeon pea and cowpea had more N uptake than sunn hemp and the double-cropped grain crops. Fallow with N fertilizer application produced significantly greater N[subscript]2O emissions than all the cover crops systems. Nitrous oxide emissions were relatively similar in the various cover crop systems and fallow with 0 kg N ha[superscript]-1. Grain yield of sorghum and maize in all the cover crop and double cropped soybean systems was similar to that in the fallow with 45 kg N ha[superscript]-1. Both grain sorghum and maize in the double-cropped soybean system and fallow with 90 kg N ha[superscript]-1 or 135 kg N ha[superscript]-1 gave profitable economic net returns over the years. The double-cropped grain sorghum system increased aggregate-associated C and whole soil total C, and all the cover crop and the double-cropped soybean systems increased aggregate-associated N and soil N pools. Inclusion of leguminous cover crops without N fertilizer application reduced N[subscript]2O emissions and provided additional C accumulation and N uptake, contributing to increased grain yield of the following cereal grain crop.

Master of Science / Department of Agronomy / J. Anita Dille / Kraig Roozeboom / Increasing numbers of herbicide-resistant weed species require alternative methods of weed suppression to be examined. This study quantified the interaction between various cover crop or herbicide systems and horseweed [Conyza canadensis (L.)] growth. Fall cover crops of winter wheat [Triticum aestivum (L.)], winter rye [Secale cereal (L.)], barley [Hordeum vulgare (L.)] and annual ryegrass [Lolium multiflorum (L.)] were seeded in November 2012 and 2013. Spring cover crop of oat [Avena sativa (L.)] was seeded in April 2013 or rye was seeded in March 2014. All cover crops were no-till seeded into grain sorghum stubble [Sorghum bicolor (L.) Moench]. Four herbicide treatments were fall or spring applied, with and without residual. The spring non-residual treatment was also applied to plots of winter rye. Cover crop plots were split and terminated with a roller crimper or glyphosate application prior to soybean [Glycine max (L.) Merr.] planting to determine the effect of termination method on treatment performance. Soybean was planted in June 2013 and May 2014 and mechanically harvested in October of both years. Horseweed density, biomass accumulation, and soybean yield data were quantified. Horseweed height, whole plant seed production, and seed subsamples were recorded in the untreated fallow control, winter wheat, and winter rye plots in 2014. Horseweed suppression by winter rye approached 90%, levels similar to suppression by herbicide systems. In both years, herbicide plots had less than half the horseweed biomass than any of the cover crop systems. In 2013, soybean yields in herbicide plots were at least 1,500 kg ha[superscript]-1, nearly more than double yields in cover crop plots. Soybean yields in 2014 were more consistent across treatments; barley and spring rye plots achieved yields equal to or greater than 2,000 kg ha[superscript]-1. Winter rye and winter wheat reduced horseweed seed production by 60% compared to the untreated fallow control, with no effect on individual seed weight. Seed production varied across plants, with the untreated control producing the greatest number of seeds. Cover crops were successful at reducing horseweed biomass, suppressing horseweed pressure, preserving soybean biomass, and protecting soybean yields when compared to a fallow untreated control.

Call number: LD2668 .T4 1961 M59

Call number: LD2668 .T4 1978 L43 / Master of Science / Chemical Engineering

Call number: LD2668 .T4 1979 M78 / Master of Science

Thesis (M.Sc.(Crop Science) North-West University, Mafikeng Campus, 2011

Thesis (MSc)--University of Stellenbosch, 2002. / ENGLISH ABSTRACT: The aim of this study was to determine (l) the influence of elevated dissolved inorganic carbon (DIC) on the nitrogen use efficiencies (NUE) of tomato seedlings grown with different nitrogen sources, (2) how changes in the regulation and activities of nitrate reductase (NR), phosphoenolpyruvate carboxylase (PEPc), carbonic anhydrase (CA) and subsequent changes in metabolites would account for observed changes in NUE, and (3) to what extent elevated DIC contributed to the carbon budget of plants grown with different nitrogen sources. Lycopersicon esculentum cv. Fl44 seedlings were grown in hydroponic culture (pH 5.8) with 2 mM of either N03- or NH4 + and the solutions were aerated with either 0 ppm or 5000 ppm CO2 concentrations. The similar NUEs of NH/-fed plants grown with either root-zone CO2 concentration were largely due to their similar RGRs and N uptake rates. Elevated root-zone DIC had an initial stimulatory effect on N~ + uptake rates, but it seems as if this effect of DIC physiological processes was cancelled out by the toxic effect of unassimilated NH/. The NUE for N03--fed plants supplied with 5000 ppm root-zone CO2 was higher relative to 0 ppm root-zone CO2 and it was possibly due to the higher relative growth rates for similar N uptake rates of 5000 ppm compared to 0 ppm root-zone CO2. Nitrate-fed plants grown with 5000 ppm compared to 0 ppm root-zone CO2 had higher in vivo NR and in vitro NR and PEPc activities. These increases in enzymes activities possibly lead to increases in organic acid synthesis, which could have been used for biomass accumulation. This would account for the increased relative growth rates of N03--fed plants grown with 5000 ppm compared to 0 ppm root-zone CO2. The increasing rootzone CO2 concentrations resulted in the Ó15N values of NH/-plants becoming more positive indicating an absence of enzymatic discrimination. This may have been due to the inhibitory effect of DIC on Nll,+ uptake, causing plants to utilise both internal isotopes equally. The Ól3C studies showed that PEPc contributed equally to both N03-- and NH/-fed plants over the long term. From this it can be concluded that the lower NUE of NH/-compared to N03--fed plants grown with 5000 ppm root-zone C02 was due to increased N uptake and exudation of organic compounds into the nutrient solution. Experiments with 813C also showed that at increasing rootzone CO2 concentrations, PEPc made a bigger contribution to the carbon budget via the anaplerotic reaction. / AFRIKAANSE OPSOMMING: Die doel van hierdie studie was om (1) die invloed van verhoogde opgeloste anorganiese koolstof dioksied (DIC) op die stikstofverbruiksdoeltreffenheid (NUE) van plante wat op verkillende stikstofbronne gekweek is, te bepaal. (2) Veranderinge in die regulering van nitraat reduktase (NR), fosfo-enolpirovaatkarboksilase (PEPc) en karboonsuuranhidrase (CA) is bestudeer en gekorreleer met waargeneemde verskille in NUE. (3) 'n Beraming van die mate waartoe verhoogde DIC bydra tot die koolstofbegroting van plante, gekweek op verskillende stikstofbronne, word bespreek. Lycopersicon esculentum cv. F144 saailinge is in waterkultuur (pH 5.8) met 2 mM N03- of NH/ gekweek en die oplossings is alternatiewelik met 0 ppm of 5000 ppm CO2 belug. Die NUEs van plante gekweek met NH/ en belug met albei C02 konsentrasies was vergelykbaar grootliks as gevolg van hulooreenkomstige relatiewe groeitempo's en Nopname. DIC het aanvanklik NH/ opname gestimuleer, maar enige latere stimulerende effek van DIC op fisiologiese prosesse was klaarblyklik uitgekanselleer deur N~ + toksiteit veroorsaak deur vertraagde assimilasie. Die NUE van plante gekweek met N03- en 5000 ppm CO2 was hoër as dié van plante gekweek met N03- en 0 ppm CO2. Dit is moontlik gekoppel aan hoër relatiewe groeitempo's teenoor onveranderde N opname tempo's. Plante gekweek met N03- en 5000 ppm CO2 het hoër in vivo NR en in vitro NR en PEPc aktiwiteite getoon as plante gekweek met N03- en 0 ppm CO2. Bogenoemde toenames in ensiem aktiwiteite word verbind met biomassa toename deur verhoogde organiese suur sintese. Dit bied 'n moontlike verklaring vir die hoër relatiewe groeitempo's van plante gekweek met N03- en 5000 ppm CO2 teenoor plante gegroei met N03- en 0 ppm CO2. Die 015N waardes van plante gekweek met NH/ en 5000 ppm CO2 was meer positief as dié van plante gekweek met Nl-l,+ en 0 ppm CO2 wat gedui het op die afwesigheid van ensiematiese diskriminasie. Dit kon as gevolg gewees het van die vertragende effek van DIC op Nl-la + opname wat daartoe sou lei dat die plante beide isotope eweveel inkorporeer. Eksperimente met ol3C het getoon dat PEPc oor 'n lang tydperk eweveel begedra het tot die koolstofbegroting van plante gekweek met beide N03- and N~+. Hiervan kan afgelei word dat die laer NUE van plante gekweek met NH4 + en 5000 ppm C02 in vergelyking met dié van plante gekweek met N03- en 5000 ppm CO2 die gevolg was van verhoogde NH/ opname en uitskeiding van aminosure in die voedingsoplossing. Eksperimente met 0"c het ook getoon dat verhoogde DIe konsentrasies die bydrae van PEPc tot die plant se koolstofbegroting laat toeneem.

Thesis (MSc)--Stellenbosch University, 2013. / ENGLISH ABSTRACT: The generally low concentrations of P and N in the soil, causes most plants to experience nutrient deficiency during their life cycle. Lupins can rely on both cluster roots and nodules for P acquisition and biological nitrogen fixation (BNF) respectively. The legume Lupinus albus is able to survive under low nutrient conditions, because it has two specialized belowground organs for the acquisition of N and P. In this regard, cluster roots increase P uptake and root nodules acquire atmospheric N2 via biological nitrogen fixation (BNF). Although these organs normally tolerates low P conditions, very little is known about their physiological and metabolic flexibility during variations in P supply. Furthermore, the resource allocation (C, N and P) between cluster roots and nodules has also been largely understudied. The aim of this investigation was therefore to determine the resource allocation, physiological and metabolic flexibility of these organs during variations in P supply. Although variation on P supply had no effect on the total biomass, there were significant differences in specialised below-ground organ allocation to cluster roots and nodule formation. Cluster root formation and the associated C-costs increased during low P supply. In contrast to the cluster root decline at high P supply, there was an increase in nodule growth allocation and corresponding C-costs. Since cluster roots were able to increase P acquisition under low P conditions, this below-ground investment may also have benefited the P nutrition of nodules. These findings provide evidence that when lupins acquire N via BNF in their nodules, there may be a trade-off in resource allocation between cluster roots and nodules. The short-term elevated P supply, caused an increased allocation of C and respiratory costs to nodules, at the expense of cluster roots. This alteration was also reflected in the increase in nodule enzyme activities related to organic acid synthesis, such as Phosphoenol-pyruvate Carboxylase (PEPC), Pyruvate Kinase (PK), Malate Dehydrogenase (NADH-MDH) and Malic Enzyme (ME). In cluster roots, the elevated P conditions, caused a decline in these organic acid synthesizing enzymes. This suggests that during short-term elevated P supply, there is a great degree of physiological and metabolic flexibility in the lupin nutrient acquiring structures. / AFRIKAANSE OPSOMMING: Die algemeen lae konsentrasies van fosfaat en stikstof in die grond , veroorsaak dat die meeste plante voedingstekorte ervaar tydens hul lewensiklus. Lupiene kan staatmaak op beide groep-wortels en wortel-knoppies vir P verkryging en biologiese stikstofbinding onderskeidelik. Die peulplant Lupinus albus is in staat om te oorleef onder lae voedings toestande , as gevolg van die twee gespesialiseerde ondergrondse organe vir die verkryging van stikstof en fosfaat. In hierdie verband verhoog groep-wortels fosfaat opname en wortel-knoppies verkry atmosferiese stikstof via biologiese stikstofbinding. Alhoewel hierdie organe normaalweg lae fosfaat toestande verdra , is baie min bekend oor hul fisiologiese en metaboliese buigsaamheid tydens variasies in fosfaat aanwending. Daar is verder ‘n tekort aan die studie van hulpbron toekenning tussen groep-wortels en wortel-knoppies. Die doel van hierdie ondersoek was dus om die toekenning van hulpbronne , fisiologiese en metaboliese buigsaamheid van hierdie organe tydens variasies in fosfaat aanwending te bepaal. Variesie in fosfaat verskaffing het geen invloed op die totale plant biomassa gehad nie, maar daar was wel ‘n beduidende verskil in gespesialiseerde ondergrondse toekenning tussen groep- wortels en wortel-knoppies. Groep-wortel vorming en die gepaardgaande koolstof koste het toegeneem met lae fosfaat verskaffing. In teenstelling met die groep-wortel daling met hoë fosfaat verskaffing, was daar 'n toename in groei van wortel-knoppies en die ooreenstemmende koolstof koste daarvan. Aangesien groep-wortels in staat was om fosfaat verkryging te verhoog onder lae fosfaat toestande, mag hierdie ondergrondse belegging bygedra het tot die voeding van wortel-knoppies . Hierdie bevindings bewys dat lupiene afhanklik van wortel-knoppies ‘n wisselwerking in toekenning van hulpbronne, tussen groep-wrotels en wortel-knoppies handaaf. Kort termyn verhoogde fosfaat aanwending veroorsaak 'n verhoogde toekenning van koolstof en respiratoriese energie na wortel-knoppiess, ten koste van groep-wortels . Hierdie verandering is ook weerspieël in die toename in wortel-knoppie ensiem aktiwiteit in verband met organiese suur sintese (PEPC PK,MDH,ME) . In groep-wortels, het die verhoogde P toestande verder 'n afname in die organiese suur produserende ensieme veroorsaak. Dit dui aan dat tydens kort termyn verhoogde P aanwending, daar 'n groot mate van fisiologiese en metaboliese buigsaamheid in die lupiene voedingstowwe verkryging strukture plaasvind.