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Three cotton (Gossypium hirsutum L.) germplasms (DP62, 84027, and 84033) were used to investigate the physiology of salt tolerance. Lines 84027 and 84033 were developed from the parental line DP62 and showed superior vigor under varying NaCl conditions (0.5 to 2.0 M) during germination and emergence. Proline levels increased in the leaves of all germplasms in response to increasing salinity. Varietal differences in proline levels did not reflect their variation in salt tolerance. Several physiological characteristics were also evaluated under non-saline condition in the greenhouse. There were no significant differences among germplasm sources for all parameters measured. However, salinity reduced transpiration rate, increased leaf diffusive resistance and leaf temperature for all lines. Ribosomal-RNA levels in all germplasms were evaluated after seeds were stressed for 24 hrs in various concentrations of NaCl and then germinated under normal conditions for 72 hrs. Ribosomal-RNA levels were inversely related to salt concentrations. Line 84033 followed by line 84027 had highest ribosomal-RNA content than the parental line DP62 when averaged over the four salt concentrations. Sodium content (ppm/g FW) and Cl⁻ content (ppm/g FW) were evaluated in microsomal and cell walls fractions as well as a cytoplasmic fraction which consisted of vacuoles, mitochondria, and plastids. The Cl⁻ ion exhibited a greater consistency in a concentration shift from one fraction to another as a function of time than did the Na⁺ ion. As a result, there may be a correlation between the drop in ribosomal-RNA and the amount of Cl⁻ in the microsomal fraction. Other parameters measured in the germinating seed were soluble protein (globulin), insoluble proteins (prolamin and glutelin) and fiber percentage. Variations within the germplasms were shown to exist. This study shows that even among lines that have been selected for salt tolerance from a single variety, the possibility exists that each of these lines may have a different mechanism to cope with salt stress.

Crop coefficients (K(c)) are a useful means of predicting how much water is needed for irrigating a crop. The crop water stress index (CWSI), on the other hand, is a means of knowing when to irrigate. Two field experiments were conducted during the summers of 1990 and 1991 at Maricopa Agricultural Center and Marana Agricultural Center, respectively, to evaluate water use (evapotranspiration, ET) of different cotton varieties, to develop crop coefficients for cotton grown in the state of Arizona, and to evaluate empirical and theoretical crop water stress indices under field conditions. For the 1990 experiment, ET from the cotton variety DPL 77 was obtained using soil water balance (SWB) and steady state heat balance (SSHB) techniques. For the 1991 experiment, ET from two cotton varieties (DPL 20 and Pima S-6) was estimated using the Bowen ratio energy balance (BREB) method and the steady state heat balance method. Reference evapotranspiration (ETᵣ) was obtained from weather stations located close to the experimental plots. Average daily ET from the SSHB measurements ranged from 8.24 to 15.13 mm and from 10.34 to 12.12 mm for the 1990 and 1991 experiments, respectively. Total ET from the SWB was approximately 19% less than the total ET estimated by the SSHB. Total ET from individual plants was well correlated with average stem area over the evaluation periods. Daily ET from the two cotton varieties (DPL20 and Pima S-6) was approximately similar when irrigation conditions were the same, but differed later by as much as 48.4% as irrigation continued for the variety Pima S-6 only. Daily ET from the BREB measurements and ETᵣ were used to develop a crop coefficient curve for cotton grown at Marana, Arizona, which had a maximum smoothed value of 1.21. A critical value of CWSI equal to 0.3 was obtained by observing the pattern of the CWSI values over well-watered and drier conditions, and from previous research. Using the developed crop coefficient curve and the CWSI should provide a useful means of scheduling irrigation for cotton grown under climatic conditions similar to those at Marana, Arizona.

The succulent, annual euhalophyte, Salicornia bigelovii was grown in 1, 10, 35, 45 and 60 ppt Instant Ocean. This range represents approximately 1/35 to nearly twice the salinity of seawater. The plants in the 4 highest salinities had common final dry weights and seed yields of about 60 and 11 g, respectively, while the 1 ppt plants had 28 and nearly 5 g, respectively. The water relations data reflected the growth and seed production of the plants. The plants in the 4 higher salinities had water potentials sufficient to generate large import gradients and osmotic potentials which contributed to substantial turgors. The 1 ppt plants had a gradient like the rest, but a very low turgor of 0.11 MPa which was barely 23% of that of the lowest of the other treatments. Higher salinities resulted in slightly greater organic and inorganic osmotica contents. Overall, these results suggest a relatively fixed genetic response to a wide range of salinities, as well as an inability to function well at very low salinities. No plant grown at 0 ppt was ever able to reproduce. Therefore, this plant is an obligate halophyte. Experiments in the plant's native coastal estuary indicated meristem water potentials fluctuate with the tides, although they remain about 1.5 MPa below the corresponding soil water potentials. The plants occupy a discrete elevational range throughout the estuary, spending about 1/3 of their daylight hours submerged, and apparently never see dryness. Phenotype differences in the estuary suggest that, within the habitat, pacing and consequent resource domination may be important parameters affecting plant size and possibly fitness. Nitrogen, which is characteristically rare in this and other estuaries, may be critical in this regard. The plants produce large quantities of glycine-betaine, which may be for simultaneous osmoticum use and nitrogen storage. Most roots occur in the first 3 inches of soil. A mechanism is proposed, based on highly efficient compartmentation at the cellular level and the shuttling of organic osmoticum across the tonoplast, by which the tidally based cyclical water potentials could be explained.

A pearl millet hybrid (Pennisetum americanum (L.) Leeke) and its two parents were evaluated for their photosynthetic rates, diffusive resistance, canopy temperature, transpiration rates, stomatal aperture and frequency, and growth responses to various irrigation levels. The experiments were conducted on Brazito sandy loam soil at The University of Arizona Campus Agricultural Center, Tucson, AZ in 1985 and 1986. A sprinkler was used to create water treatments. Photosynthetic rates were not significantly different among genotypes at each water level. Transpiration rate, diffusive resistance, canopy minus ambient temperature, and photosynthetic rate were all significantly related to water treatments with correlation coefficients .ranging from 0.91 to 0.98. Under water stress, the female transpired more, exhibited lower diffusive resistance and had a cooler canopy compared to the hybrid and male parent. Based on these characteristics, the female seemed to expend more energy on heat dissipation than yield improvement. Water stress reduced stomatal aperture but increased stomata! frequency. The hybrid had significantly higher stomatal frequency at all water levels and smaller aperture between 63 and 125 mm irrigation levels. Dry matter, leaf area, leaf area index, and plant height were also reduced by water stress. In general, the hybrid and the male parent produced significantly more dry matter and were significantly taller than the female. The female parent had significantly higher leaf area and leaf area index late in 1986. Grain yield was reduced by water stress. Both years, the female grown under optimum moisture conditions had approximately half the grain yield as compared to the male and the hybrid. The major components contributing to the higher grain yield of the hybrid and male were larger panicles and more productive tillers.

Refuges of non-Bacillus thuringiensis (Bt) cotton are used to delay Bt resistance in several key insect pests. In 2004, I discovered that Bt cotton plants sometimes enter refuges via the seed bag, and hypothesized that this type of gene flow could have important effects on resistance evolution in insect pests. In the research described herein, I investigated the sources of Bt plants in the non-Bt cotton seed supply and assessed the potential implications of this gene flow on pest resistance evolution. I report results from an empirical study of gene flow in 15 non-Bt cotton seed production fields, as well as results from simulation modeling studies of gene flow from one-toxin and two-toxin Bt cotton. The current policy on gene flow from genetically engineered crops in the United States is also reviewed, including the implications of my research findings for policymakers. Key findings of this study included the prominent role of seed-mediated gene flow in the seed-production setting, and the utility of a geographic information system (GIS) ring analysis approach for describing pollen-mediated gene flow in cotton fields. Modeling results indicated that high rates of gene flow of Bt cotton into refuges could have large effects on pest resistance evolution under certain sets of assumptions, particularly in parts of the world where farm-saved seed is planted year after year in cotton fields. It appears that some of these effects could be mitigated by using non-cotton refuges or by using plants that contain linked transgenes that confer multiple toxins. There are no clear regulations in the United States regarding gene flow of Bt cotton into refuge seed or into seed production fields of non-Bt cotton, as Bt cotton has been deregulated following extensive safety testing. Nevertheless, results from this research suggest that limiting gene flow into refuge seed could be important for sustaining the efficacy of Bt cotton against targeted insect pests in regions where refuges are used.

This item was digitized as part of the Million Books Project led by Carnegie Mellon University and supported by grants from the National Science Foundation (NSF). Cornell University coordinated the participation of land-grant and agricultural libraries in providing historical agricultural information for the digitization project; the University of Arizona Libraries, the College of Agriculture and Life Sciences, and the Office of Arid Lands Studies collaborated in the selection and provision of material for the digitization project.

This item was digitized as part of the Million Books Project led by Carnegie Mellon University and supported by grants from the National Science Foundation (NSF). Cornell University coordinated the participation of land-grant and agricultural libraries in providing historical agricultural information for the digitization project; the University of Arizona Libraries, the College of Agriculture and Life Sciences, and the Office of Arid Lands Studies collaborated in the selection and provision of material for the digitization project.

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Two pinto bean variety trials were grown in Greenlee and southern Graham counties. UI 114, the most commonly grown variety in both areas, was out yielded in both locations by other varieties. In Greenlee county, Luna, a New Mexico variety, surpassed UI 114 by approximately 100 pounds per acre. In Graham county, Olathe, a Colorado variety, out yielded UI 114 by more than 250 pounds per acre. At $40 per hundredweight, the latter difference translates into more than $100 per acre.