The purpose of this study was to evaluate a programme aimed at equipping adult mine workers in the North-West Province in computer-based skills. The study describes how the use of computers for learning has transformed some of the mine workers in an Own-Time learning environment, where computers were integrated in their learning activities, compared to the Full-Time adult learning environment, which did not present a similar opportunity. The perspective of using a combination of two teaching methodologies in the Adult Education and Training (AET) curriculum provided at this mine sought to minimise the conventional didactic instructional approaches, whereby the facilitator does most of the talking while learners become passive listeners. Activity theory (AT), in tandem with the reviewed literature, was well suited as a theoretical framework for this study, since it takes a developmental view of minds and technological interaction in context. The activity system was used to map how existing work-related practices fitted into each component, which includes the subject, object, rule, community and division of labour. A case study was applied as a guiding design in the study in which seventeen mine workers participated. The computer activity was illustrated from multiple perspectives, using a qualitative approach and associated epistemologies and beliefs regarding the engagement with mediational tools. Boundary crossings, the zone of proximal development, and the eight-step-model from activity theory were used to scrutinise the learners’ beliefs, attitudes, knowledge, practices and contradictions. These important aspects assisted in obtaining data through participant observation, interviews and field notes. To discover patterns, concepts, themes and meanings from the notes and transcripts, data analysis was blended with these aspects. This study demonstrated the use of the above-mentioned methods at appropriate times. Without ideal and effective research methods, the quality of evidence of this study would have suffered and elucidations of connections would have been inadequate. The findings revealed that the AET programme made an impact on the company and on the individual participants, in their homes, their place of work, and their communities. Change in the workers’ performance after attending AET computer-based programmes was noticeable especially regarding their literate and numerate knowledge and skills. Contradictions and disturbances in the AET programme were also identified, and an intervention was proposed to facilitate a computer programme that includes the curriculum components such as mouse skills, keyboarding, data manipulation and desktop publishing. The study proposes that the AET policy needs to cover Own-Time learners, even though their learning is not obligatory, in the same way as it covers the Full-Time learners. A modified activity theory, contextualised for a situation such as that in the mine in question, was contributed in the study. Furthermore, some limitations and recommendations for future research were identified from the findings. The proposed AT for adult learners suggests a new way of exploring the practices of mind and technology interaction for adult learners, incorporating cultural backgrounds, age, language, values and beliefs as well as reflective decision making. / Science and Technology Education / D. Ed. (Comparative Education)

Syfte – Studien syftade till att undersöka den interna interaktionen i en liten, tillverkande ETO-verksamhets kundorderprocess samt hur boundary object underlättar dess gränsöverskridande interaktion. För att uppfylla studiens syfte kommer följande frågeställningar att besvaras: Vad kännetecknar den gränsöverskridande interaktionen i en liten, tillverkande ETO-verksamhets kundorderprocess? Hur kan boundary object underlätta den gränsöverskridande interaktionen i en liten, tillverkande ETO-verksamhet? Metod – För att uppfylla studiens syfte har en fallstudie genomförts hos Premould AB. Via intervjuer och dokumentstudier har deras kundorderprocess undersökts och interaktionsobjekt i processen identifierats. Insamlad data låg sedan till grund för hur det teoretiska ramverket upprättades. Med hjälp av den empiriska insamlingen och det teoretiska ramverket har en analys genomförts som i sin tur genererat studiens resultat. Resultat – Genom att undersöka kundorderprocessen, i en ETO-verksamhet, har olika kännetecken för den gränsöverskridande interaktionen kunnat urskiljas. Interaktionsobjekt, som belyses som boundary object, har identifierats i processen. Boundary object kan underlätta den interna interaktionen om de används utefter den nivå av komplexitet av kunskapsridning som finns i gränssnitten mellan funktioner. Implikationer – Resultatet att boundary object underlättar den gränsöverskridande interaktionen ger ett praktiskt bidrag till små, tillverkande ETO-verksamheter. Genom valet av boundary object ska det gå att förenkla kunskapsspridning och genom det effektivisera arbetsprocesser. Det ges även ett teoretiskt bidrag till vidare forskning om boundary object i ETO-kontexter. Begränsningar – En av begränsningarna i studien är att det kan vara svårt att generalisera utifrån resultatet eftersom studien var av enfallsdesign. Gränsöverskridande interaktion är dessutom komplext att undersöka med strukturella metoder och det saknades därför möjlighet att djupt undersöka de identifierade interaktionsobjekten. Nyckelord – Engineer to order (ETO), gränsöverskridande interaktion, kunskapsgränser, kunskapsspridning, boundary object. / Purpose – The purpose of the study was to investigate the internal interaction of a small, manufacturing ETO-business's customer order process, as well as how boundary objects facilitate its boundary-crossing interaction. In order to fulfill the purpose of the study the following questions will be answered: What characterizes boundary-crossing interaction in a small, manufacturing ETO-business's customer ordering process? How can boundary objects facilitate boundary-crossing interaction in a small, manufacturing ETO-business? Method – To fulfil the purpose of the study, a case study has been conducted at Premould AB. Through interviews and document studies, their customer order process has been investigated and interaction objects in the process been identified. The collected data then formed a base for the establishment of the theoretical framework. With the empirical collection and the theoretical framework, an analysis has been conducted which, in turn, generated the results of the study. Findings – By examining the customer ordering process, in an ETO-business, different characteristics of boundary-crossing interaction has been identified. Interaction objects, which are used according to the principles of boundary objects, have been identified in the process. Boundary objects can facilitate internal interaction if used at the proper level of complexity of knowledge sharing found in the interfaces. Implications – The result that boundary objects facilitate boundary-crossing interaction provides a practical contribution to small, manufacturing ETO-business. Through the choice of boundary object used, it is possible to simplify knowledge sharing and make more effective work processes. There is also a theoretical contribution to further research on boundary objects in ETO-contexts. Limitations – One of the limitations of the study is that it can be difficult to generalize from the result, due to the use of a single-case design. Moreover, boundary-crossing interaction is complex to investigate with structural methods, and it was therefore difficult to closely investigate the identified objects. Keywords – Engineer to order (ETO), boundary-crossing interaction, knowledge boundaries, boundary object.

South Africa has had a free mandate theory of representation up to 1994. From 1994 to 2002 an imperative theory applied and in 2003 a limited hybrid free mandate was introduced. The origin of parliament, the development of representation as a concept in Public Law and the birth of political parties are studied. It is shown that parliament and representation were natural developments that occurred at the same time, not by grand design, but by chance. It is also shown that political parties appeared first as informal intra-parliamentary groupings that developed into extra-parliamentary organisations, organised to achieve and exercise power in the political system as the franchise became more liberal. The factors that influence a member's mandate and floor-crossing as such are discussed. Finally it is concluded that from a legal historical perspective, a free mandate of representation is the preferred theory of representation in public law. / Jurisprudence / LL.M. (Public Law)

The characterization of the Markan disciples has been and continues to be the object of much scholarly reflection and speculation. For many, the Markan author's presentation of Jesus' disciples holds a key, if not the key, to unlocking the purpose and function of the gospel as a whole. Commentators differ as to whether the Markan disciples ultimately serve a pedagogical or polemical function, yet they are generally agreed that the disciples in Mark come off rather badly, especially when compared to their literary counterparts in Matthew, Luke, and John. This narrative-critical study considers the characterization of the Markan disciples within the Sea Crossing movement (Mark 4:1-8:30). While commentators have, on the whole, interpreted the disciples' negative characterization in this movement in terms of lack of faith and/or incomprehension, neither of these, nor a combination of the two, fully accounts for the severity of language leveled against the disciples by the narrator (6:52) and Jesus (8:17-18). Taking as its starting point an argument by Jeffrey B. Gibson (1986) that the harshness of Jesus' rebuke in Mark 8:14-21 is occasioned not by the disciples' lack of faith or incomprehension but by their active resistance to his Gentile mission, this investigation uncovers additional examples of the disciples' resistance to Gentile mission, offering a better account of their negative portrayal within the Sea Crossing movement and helping explain many of their other failures. In short, this study argues that in Mark 4:1-8:26, the disciples are characterized as resistant to Jesus' Gentile mission and to their participation in that mission, the chief consequence being that they are rendered incapable of recognizing Jesus' vocational identity as Israel's Messiah (Thesis A). This leads to a secondary thesis, namely, that in Mark 8:27-30, Peter's recognition of Jesus' messianic identity indicates that the disciples have finally come to accept Jesus' Gentile mission and their participation in it (Thesis B). Chapter One: Introduction: offers a selective review of scholarly treatments of the Markan disciples, which shows that few scholars attribute resistance, let alone purposeful resistance, to the disciples. Chapter Two: The Rhetoric of Repetition: introduces the methodological tools, concepts, and perspectives employed in the study. It includes a section on narrative criticism, which focuses upon the story-as-discoursed and the implied author and reader, and a section on Construction Grammar, a branch of cognitive linguistics founded by Charles Fillmore and further developed by Paul Danove, which focuses upon semantic and narrative frames and case frame analysis. Chapter Three: The Sea Crossing Movement, Mark 4:1-8:30: addresses the question of Markan structure and argues that Mark 4:1-8:30 comprises a single, unified, narrative movement, whose action and plot is oriented to the Sea of Galilee and whose most distinctive feature is the network of sea crossings that transport Jesus and his disciples back and forth between Jewish and Gentile geopolitical spaces. Following William Freedman, Chapter Four: The Literary Motif: introduces two criteria (frequency and avoidability) for determining objectively what constitutes a literary motif and provides the methodological basis and starting point for the analyses performed in chapters five and six. Chapter Five: The Sea Crossing Motif: establishes and then carries out a lengthy narrative analysis of the Sea Crossing motif, which is oriented around Mark's use of ‎θάλασσα (thalassa) and πλοῖον (ploion), and Chapter Six: The Loaves Motif: does the same for The Loaves motif, oriented around Mark's use of ἄρτος (artos). Finally, Chapter Seven: The Narrative Logic of the Disciples (In)comprehension: draws together all narrative, linguistic, and exegetical insights of the previous chapters and offers a single coherent reading of the Sea Crossing movement that establishes Theses A and B.

Cette thèse traite de processus de détérioration en théorie du risque et en biomathématique.<p><p>En théorie du risque, le processus de détérioration étudié est celui des sinistres supportés par une compagnie d'assurance.<p><p>Le premier chapitre examine la distribution de Markov-Polya comme loi possible pour modéliser le nombre de sinistres et établit certains liens avec la famille de lois de Katz/Panjer. Nous construisons la loi de Markov-Polya sur base d'un modèle de survenance des sinistres et nous montrons qu'elle satisfait une récurrence élégante. Celle-ci permet notamment de déduire un algorithme efficace pour la loi composée correspondante. Nous déduisons la famille de Katz/Panjer comme famille limite de la loi de Markov-Polya.<p><p>Le second chapitre traite de la famille dite "Lagrangian Katz" qui étend celle de Katz/Panjer. Nous motivons par un problème de premier passage son utilisation comme loi du nombre de sinistres. Nous caractérisons toutes les lois qui en font partie et nous déduisons un algorithme efficace pour la loi composée. Nous examinons également son indice de dispersion ainsi que son comportement asymptotique. <p><p>Dans le troisième chapitre, nous étudions la probabilité de ruine sur horizon fini dans un modèle discret avec taux d'intérêt positifs. Nous déterminons un algorithme ainsi que différentes bornes pour cette probabilité. Une borne particulière nous permet de construire deux mesures de risque. Nous examinons également la possibilité de faire appel à de la réassurance proportionelle avec des niveaux de rétention égaux ou différents sur les périodes successives.<p><p>Dans le cadre de processus épidémiques, la détérioration étudiée consiste en la propagation d'une maladie de type SIE (susceptible - infecté - éliminé). La manière dont un infecté contamine les susceptibles est décrite par des distributions de survie particulières. Nous en déduisons la distribution du nombre total de personnes infectées à la fin de l'épidémie. Nous examinons en détails les épidémies dites de type Markov-Polya et hypergéométrique. Nous approximons ensuite cette loi par un processus de branchement. Nous étudions également un processus de détérioration similaire en théorie de la fiabilité où le processus de détérioration consiste en la propagation de pannes en cascade dans un système de composantes interconnectées. <p><p><p> / Doctorat en Sciences / info:eu-repo/semantics/nonPublished

[pt] IoT refere-se a um sistema de dispositivos de computação inter-relacionados que visa transferir dados através de uma rede sem exigir interação humanohumano ou humano-para-computador. Esses sistemas de comunicação modernos, exigem restrições de baixo consumo de energia e baixa complexidade no receptor. Nesse sentido, o conversor analógico-digital representa um gargalo para o desenvolvimento das aplicações dessas novas tecnologias, pois apresenta alto consumo de energia devido à sua alta resolução. A pesquisa realizada em relação aos conversores analógico-digitais com quantização grosseira mostrou que esses dispositivos são promissores para o projeto de futuros sistemas de comunicação. Para equilibrar a perda de informações, devido à quantização grosseira, a resolução no tempo é aumentada através da superamostragem. Esta tese considera um sistema com quantização de 1 bit e superamostragem no receptor com um canal de downlink MIMO multiusuário com banda ilimitada e apresenta, como principal contribuição, a nova modulação de cruzamento de zeros que implica que a informação é transmitida no instante de tempo zero-crossings. Este método é usado para a pré-codificação temporal através da otimização do design da forma de onda para dois pré-codificadores diferentes, a maximização temporal da distância mínima até o limiar de decisão com forçamento a zero espacial e a pré-codificação MMSE no espácio-temporal. Os resultados da simulação mostram que a abordagem de cruzamento de zeros proposta supera o estado da arte em termos da taxa de erro de bits para os dois pré-codificadores estudados. Além disso, essa nova modulação reduz a complexidade computacional, permite dispositivos de complexidade muito baixa e economiza recursos de banda em comparação com o método mais avançado. Análises adicionais mostram que a abordagem do cruzamento de zeros é benéfica em comparação com o método mais avançado em termos de maior distância mínima até o limiar de decisão e menor MSE para sistemas com limitações de banda. Além disso, foi desenvolvido um esquema de mapeamento de bits para modulação de cruzamento por zero, semelhante à codificação de Gray para reduzir ainda mais a taxa de erro de bits. / [en] The IoT refers to a system of interrelated computing devises which aims to transfer data over a network without requiring human-to-human or humanto- computer interaction. This Modern communication systems demand restrictions of low energy consumption and low complexity in the receiver. In this sense, the analog-to-digital converter represents a bottleneck for the development of the applications of these new technologies since it has a high energy consumption due to its high resolution. The research carried out concerning to the analog-to-digital converters with coarse quantization has shown that such devices are promising for the design of future communication systems. To balance the loss of information, due to the coarse quantization, the resolution in time is increased through oversampling. This thesis considers a system with 1-bit quantization and oversampling at the receiver with a bandlimited multiuser MIMO downlink channel and introduces, as the main contribution, the novel zero-crossing modulation which implies that the information is conveyed within the time instant of the zero-crossings. This method is used for the temporal precoding through the waveform design optimization for two different precoders, the temporal maximization of the minimum distance to the decision threshold with spatial zero forcing and the space-time MMSE precoding. The simulation results show that the proposed zero-crossing approach outperforms the state-of-theart in terms of the bit error rate for both precoders studied. In addition, this novel modulation reduces the computational complexity, allows very low complexity devices and saves band resources in comparison to the state-ofthe- art method. Additional analyses show that the zero-crossing approach is beneficial in comparison to the state-of-the-art method in terms of greater minimum distance to the decision threshold and lower MSE for systems with band limitations. Moreover, it was devised a bit-mapping scheme for zero-crossing modulation, similar to Gray-coding to further reduce the bit error rate.

<p>The crossing of host barriers (intestinal, blood-brain, and placental) is a critical step for systemic infections caused by entero-invasive pathogens. In the intestine, the epithelial cells are the first line of defense against enteric pathogens. <i>Listeria monocytogenes</i> is a facultative-intracellular foodborne pathogen that first crosses the intestinal barrier to cause a systemic infection. However, the underlying mechanism is not well understood.</p><p><br></p> <p>We demonstrate that <i>Listeria</i> adhesion protein (LAP) promotes the translocation of <i>L. monocytogenes </i>across the intestinal barrier in mouse models (A/J and C57BL/6). Relative to the wild-type (WT; serotype 4b) or the isogenic bacterial invasion protein Internalin A mutant (Δ<i>inlA</i>) strain, the <i>lap^─</i> strain showed significant defect in translocation across the intestinal barrier and colonization of the mesenteric-lymph nodes, liver and spleen in the early phase of infection (24 h and 48 h). LAP induces intestinal epithelial barrier dysfunction for increased translocation as evidenced by increased permeability to 4-kDa FITC-dextran (FD4), a marker of paracellular permeability, in the serum and urine of WT and Δ<i>inlA</i>- infected mice and across Caco-2 cell barrier, but not the <i>lap^─</i> mutant strain. Microscopic examination confirmed localization of the WT and Δ<i>inlA</i> strains in the tight junction, a crucial barrier of intestinal paracellular permeability, in the mouse ileal tissue but the <i>lap^─</i> strain remained confined in the lumen. LAP also upregulates TNF-α and IL-6 in intestinal epithelia of mice and in Caco-2 cells for increased permeability. </p><p><br></p> <p>Investigation of the underlying molecular mechanisms of LAP-mediated increase in intestinal permeability by using <i>lap^─</i> mutant strain, purified LAP and shRNA-mediated Hsp60 suppression, we demonstrate that LAP interacts with its host receptor, Hsp60, and activates the canonical NF-κB signaling, which in turn facilitates myosin light-chain kinase (MLCK)-mediated opening of the epithelial barrier via the cellular redistribution of major epithelial junctional proteins claudin-1, occludin, and E-cadherin. Pharmacological inhibition of NF-κB or MLCK in cells or genetic ablation of MLCK in mice (C57BL/6) prevents mislocalization of epithelial junctional proteins, intestinal permeability and <i>L. monocytogenes</i> translocation across the intestinal barrier.</p> <p><br></p><p>Furthermore, LAP also promotes <i>L. monocytogenes </i>translocation across the intestinal barrier and systemic dissemination in a Mongolian gerbil that are permissive to the bacterial invasion proteins; InlA-and InlB-mediated pathways; similar to that in humans. We show a direct LAP-dependent and InlA-independent pathway<i> </i>for <i>L. monocytogenes</i> paracellular translocation across the intestinal epithelial cells that do not express luminally accessible E-cadherin. Additionally, we show a functional InlA/E-cadherin interaction pathway that aids <i>L. monocytogenes</i> translocation by targeting cells with luminally accessible E-cadherin such as cells at the site of epithelial cell extrusion, epithelial folds and mucus-expelling goblet cells. Thus, <i>L. monocytogenes</i> uses LAP to exploit epithelial innate defense in the early phase of infection to cross the intestinal epithelial barrier, independent of other invasion proteins.</p><p><br></p> <p>This work fills a critical gap in our understanding of <i>L. monocytogenes </i>pathogenesis and sheds light to the complex interplay between host-pathogen interactions for bacterial crossing of the crucial intestinal barrier.</p> <br>

The doctoral thesis analyses driver’s conduct while solving situations associated with crossing of pedestrians across the road in cases of various design of pedestrian crossings and at different conditions. The thesis deepens the knowledge of the human factor impact on the occurrence of a traffic accident involved vehicle and pedestrian and thesis also complements knowledge for the needs of the analysis of traffic accidents, especially in solving the pre collision phase and at assessment of possibilities for collision prevention by involved participants. The thesis deals with driver’s conduct, various models of the conduct as well as the thesis focuses on the visual perception, the process of information processing, the driver’s conduct and the reaction time. In terms of the solution suitable types of experiments were designed and implemented. Based on performed measurements a method of processing and evaluating data on drivers’ conduct was found as well as more significant data set was obtained for a detailed analysis of drivers' conduct in different driving situations. The assessed quantities of drivers' conduct were analysed with respect to the dangerousness of driving situations. For these purposes, the categories of the dangerousness of driving situations were defined (situations completely safe, with increased danger, dangerous and critical), into which the analysed driving situations were subsequently included. To enable the quantification of this classification of situations into the categories of the dangerousness, the coefficient of the dangerousness (so called K) was defined. From the detailed analysis of the obtained data, the limit values of this coefficient were determined, and these were subsequently verified using data from the solution of real traffic accidents. Concurrently the analysis verified the suitability of using this hazard coefficient in the analysis of traffic accidents, especially for a detailed assessment of the possibilities of collision prevention.

Aim of this Master´s thesis is the road adjustment and design of new grade separated junction highway R43 and road I/43,of Černá Hora – Svitávka and to design optimal distribution of transport hubs including the design II/150 and III/37429, which should be connected with existing roads.The adjustment of the routes will be designed in the necessary extent with the optimal distribution of transport hubs regarding the best transport and economical solution and with minimal impact on the enviroment.

The thesis deals with design of the tram power collecting route and the tram track of the route no. 3 in reconstruction area recently called “Zbrojovka Brno”. Connection to the present infrastructure is also considered. Housing, resting, administration sectors and tram stops are designed for easy availability in the new area. Because the railroad bounds the area on the East and will be extended in the future, joining the tram transport with integrated transport system in Židenice is possible. Design includes the new bridge across Svitava river and connection with surrounding areas. If we divide individual car transport (IAD) from the tram route, the trolley car transportation is designed in place IAD.