This thesis is focused on the issue of tourism planning within the context of sustainable competitive advantage which can be reached by an engagement of regional stakeholders into the phase of strategy implementation. Its aim is to evaluate cooperation between regional destination management organization and destination's stakeholders during the implementation process of strategic priorities defined in the Tourism Development Strategy in the South Bohemia Region. The thesis utilizes information from representatives of the destination management organization and regional stakeholders to be able to realize so-called Stakeholder analysis. It analyses the destination stakeholders from the point of view of free key attributes. It also pays its attention to the forms of their involvement in the planning of tourism development and their experience with this process. On this basis it formulates the basis for future actions which should lead to an improvement of the current state of cooperation with key regional stakeholders.