This work deals with part of the encryption algorithm, called S-box and its development. For its development is used evolutionary computing, such as classical genetic algorithm, Estimation of Distribution Algorithm, Cartesian genetic programming and multi-criteria VEGA and SPEA algorithms. This thesis aims to test the properties of substitution boxes to its evolutionary development. Firstly, the work deals with cryptography and issues of s-boxes. There are explained basic concepts and describes the selected criteria of safety. Next chapter explains evolutionary algorithms   and multi-criteria optimization. This knowledge is used to design and program implementation, which are described below. Finally discusses the application of the criteria studied. Discussed here is searching S-boxes in both single-criteria, and especially in multi-criteria genetic search.