Made available in DSpace on 2016-12-08T16:24:16Z (GMT). No. of bitstreams: 1 PGCA13MA120.pdf: 38413 bytes, checksum: d9d09a49a28d09946e0666448617c0b5 (MD5) Previous issue date: 2013-02-22 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / Hovenia dulcis Thunberg (Grape Japan) is a deciduous tree native of China and some parts of Japan. Over the last years, this plant has been used as form of shading in aviary in western Santa catarina and began to spread across the region. The cattle eat the ripe fruit of this plant when they fall to the ground. The fruit is suspected of poisoning, annually in the fall and early winter, period that coincide with the fruit maturation. A single dose of 24,5 g/Kg reproducer the disease in a experimental conducted in 2004. In subsequent years, there were no complaints about the toxicity of the fruits of this plant, although many breeders said the cattle, during the season of ripening, continued to eat the fruits. New experiments were conducted in the years 2011 and 2012 in order to evaluate experimentally the variation of toxicity of fruits Grape Japan , the amount needed to produced poisoning in cattle and compare the experimental clinical signs with spontaneous disease. Fruits were administered at single adoses ranging from 30 to 50 g/Kg to calves, only two bovine become seriously ill one died. The clinical and lesion were similar to experimentally reproduced by other authors in 2004, however, the dose required to reproduce the experimental disease was 100% higher than the toxic dose recommended as lethal in 2004 / Hovenia dulcis Thunberg (Uva Japão) é uma árvore caducifólia nativa da China e de alguns lugares do Japão. Nos últimos anos essa planta foi utilizada como forma de sombreamento para aviários no Oeste de Santa Catarina e passou a disseminar-se por toda a região. Os bovinos comem avidamente os frutos maduros dessa planta quando caem ao chão. Suspeitas de intoxicação pelos frutos ocorrem anualmente no outono e início de inverno, que coincidem com a maturação dos frutos. A doença foi reproduzida experimentalmente em 2004 através da administração dos frutos para bovinos em dose única, a partir de 24,5 g/Kg. Nos anos subsequentes não ocorreram reclamações sobre a intoxicação pelos frutos dessa planta, embora, muitos criadores afirmavam que os bovinos, na temporada de maturação, continuavam a ingerir os frutos. Com o objetivo de avaliar experimentalmente a variação da toxicidade de Uva Japão , a quantidade necessária para produzir intoxicação em bovinos e comparar o quadro clínico experimental com a doença espontânea. Novos experimentos foram conduzidos nos anos de 2011 e 2012. Dos 11 bovinos que receberam frutos da planta em doses únicas entre 30 e 50 g/Kg, apenas dois bovinos adoeceram gravemente e um morreu. O quadro clínico e lesional foram semelhantes ao reproduzido experimentalmente por outros autores em 2004, porém, a dose necessária para reproduzir a doença experimental foi 100% superior a dose tóxica preconizada como letal em 2004

Memoria para optar al título profesional de Ingeniero Agrónomo Mención Fruticultura / El almendro es una especie predominantemente autoincompatible entre algunas de sus variedades, lo que obliga a los productores a establecer huertos con al menos dos variedades intercompatibles. Esta incompatibilidad actúa para prevenir la autofecundación, la que es del tipo gametofítica, por la expresión de proteínas específicas dentro de los estilos. Es por esto que es de suma importancia conocer la compatibilidad entre las variedades, existiendo diversos métodos para determinarla. En los últimos años, se ha establecido la compatibilidad a través de evaluaciones moleculares, que utilizan técnicas específicas para determinar el genotipo S de cada variedad, y han existido avances importantes en el desarrollo de técnicas biológicas para la determinación de la compatibilidad. Durante la floración del año 2011, con las variedades de almendro Desmayo Largueta, Desmayo Rojo, Nonpareil, Fritz, Wood Colony, Carmel, Thompsom, Sonora, Solano, Masbovera, Francolí, Price, Ne plus Ultra, Glorieta y Marcona, se utilizó la técnica propuesta por Mori et al. (2006), basada en el protocolo del laboratorio Daphne Preuss, para determinar la compatibilidad entre ‘Marcona’ y 14 variedades de almendro. Esta técnica está basada en la tinción de la callosa de los tubos polínicos con anilina azul, permitiendo la observación del crecimiento, utilizando microscopio de fluorescencia. Además, se realizó un seguimiento de los estados fenológicos de las variedades analizadas, en campo, determinando que Desmayo Rojo, Thompson, Glorieta, Masbovera y Francolí coincidieron en menos de 5 días con la floración de ‘Marcona’. Se determinó la compatibilidad de todas las variedades con Marcona, resultando compatible con las 14 variedades ensayadas. Los resultados concuerdan con los análisis genéticos moleculares encontrados en diversos estudios, validando a este método como una herramienta para determinar compatibilidad, sobre todo en variedades donde aún los alelos S no han sido identificados. / Almond cultivars are predominantly self-incompatible which requires to plant at least two inter-compatible varieties. This incompatibility acts to prevent self-fertilization, which is the gametophytic type given by specific proteins produced in the styles. Because of this, knowing the compatibility between cultivars is required and different methods to determine it have been established in recent years through molecular or biological techniques. During the 2011 flowering, the compatibility between Marcona and the cultivars Desmayo Largueta, Desmayo Rojo, Nonpareil, Fritz, Wood Colony, Carmel, Thompsom, Sonora, Solano, Masbovera, Francolí, Price, Ne plus Ultra and Glorieta, were analyzed using the technique developed by Mori et al. (2006), based on Daphne Preuss laboratory protocol. This technique stains callose using aniline blue, allowing pollen tube growth to be observed with flourescence microscopy. Furthermore, flowering of analized cultivars was monitored in the field, showing that Desmayo Rojo, Thompson, Glorieta, Masbovera and Francolí cultivars coincided in less than five days with Marcona cultivar. Marcona was compatible with all studied cultivars, in accordance with genetic molecular analysis found in different studies. Also, these results validated this method as useful to determine genetic compatibility, especially for cultivars where the S alleles have not been identified yet.

Almond (Prunus dulcis) is the most important nut crop in terms of world production. Due to its health benefit and high nutritional value the consumption and world supply of almond is increasing. To remain competitive in the world market, the Australian almond breeding program was established to produce cultivars with better adaptation to Australian conditions. As part of this program an almond mapping population consisting of 93 F₁ progeny derived from a cross between the American cultivar ‘Nonpareil’ (NP) and the European self-compatible cultivar ‘Lauranne’ (LA) was produced to construct the genetic linkage maps. The first almond linkage map developed prior to the commencement of this project failed to produce the eight linkage groups similar to the basic chromosome number of almond (x = 8) and many large gaps were also observed on the linkage groups. Therefore, more markers were needed to saturate the maps. Microsatellite markers are considered one of the best choices for mapping studies. 195 microsatellite markers isolated from Prunus species were obtained from published papers or by personal communication. Polymorphism was revealed by three different methods, and in general, polyacrylamide gel electrophoresis (PAGE) compared to the fluorescent labelled marker detection using an automated DNA sequencer or agarose gel electrophoresis, showed the most efficient and cost effective method of genotyping. A subset of 54 markers which produced reliable and easily interpretable polymorphic bands was selected to screen the whole mapping population. Microsatellites originally isolated from almond species showed the highest rate of amplification and polymorphism followed by peach microsatellites and the least informative markers were isolated from cherry. It seems that the level of transportability and usefulness of microsatellite markers is related to the genetic distance of the closely related species. Almond and peach belong to the same subgenus (Amygdalus) and other Prunus species are classified in Prunophora subgenus. The nut, or kernel, is the commercial part of the almond tree, thus to improve the quality of fruit an understanding of environmental influence, heritability and correlation of traits is required. Pomological and quality characters such as: shell hardness, kernel size, shape, taste, pubescence, colour, and percentage of doubles were measured during three consecutive years (2005-2007) on the total mapping population, but data analysis (ANOVA) was performed only on trees that survived for all three years. Most of the traits showed high broad-sense heritability and kernel shape showed the highest heritability of H² = 0.92 suggesting high genetic control of this trait. Occasionally larger kernels than either parent were found in the progeny indicating potential for improvement of this trait even with smaller kernel size parent that encompass many desirable characters. High correlation was also found between the in-shell and kernel weight (r = 0.74), kernel length / kernel width (r = 0.67), kernel weight to kernel length (r = 0.78) and kernel width (r = 0.80). This correlation estimation pointed out in this study indicates that the improvement of one character may result the progress in another trait. Neither of the parents in the mapping population had bitter or obvious slightly bitter taste but slightly bitter kernels were observed among the progeny. Amygdalin was assumed to be responsible for bitter taste in almond; therefore we measured the amount of amygdalin in sweet and slightly bitter kernel progeny by HPLC. However, the results showed that amygdalin exists in sweet kernels as well. Although the average amount of amygdalin in slightly bitter kernels (20.34 mg kg⁻¹ FW) was higher than sweet kernels (3.67 mg kg⁻¹ FW), some sweet kernels had higher amounts of amygdalin suggesting the impact of other components on slightly bitter kernel. The highest variability within the traits was observed in the percentage of double kernel, which showed the highest standard error. Strong environmental effects, particularly low temperature at pre-blossom time is speculated to produce much higher double kernels. Three genetic linkage maps, one for each parent and an integrated map were constructed by the addition of 54 new microsatellite markers to the previous dataset. All the data was scored and coded according to the coding system necessary by JoinMap3 which was used for map construction. 131 markers including microsatellite, ISSR, RAPD, SCAR and S-allele markers were placed on the integrated map covering 590.7 cM with the average density of 4.5 cM/marker. The minimum number of six microsatellite markers was placed on linkage group 8 and the linkage group 1 which is the longest linkage group has 14 microsatellite markers. Comparative mapping study with other Prunus maps, especially with the highly saturated reference map showed complete synteny and minor changes in the order of four markers on linkage groups compared with Prunus reference map. The conservation of molecular marker order observed in this study supports the idea of looking at Prunus genome as a single genetic system and practical application of this similarity would be in cross-transportability of microsatellite markers from well developed linkage maps to the less studied species in Prunus. Ten microsatellite loci placed on our map have not been reported before and could be used to improve the density of other Prunus maps, especially the reference map. This study contributed to the better understanding of the mode of inheritance and environmental effect on morphological traits and the effect of amygdalin on kernel taste. The most saturated microsatellite based almond linkage map developed in this study can serve as a framework for future almond breeding program in Australia and benefit Prunus improvement programs internationally. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1348850 / Thesis (Ph.D.) - University of Adelaide, School of Agriculture, Food and Wine, 2008

Objetivo: Determinar el efecto neuroprotector de la administración de la semilla de Prunus dulcis “almendra” sobre el tejido nervioso en ratones inducidos a estrés por desorientación motora. Diseño: Estudio analítico, transversal, experimental y prospectivo. Lugar: Laboratorios del Centro de Investigación de Bioquímica y Nutrición Alberto Guzmán Barrón, Facultad de Medicina, UNMSM, Lima, Perú. Materiales: Ratones albinos BALB/c (Mus musculus) machos y Prunus dulcis “almendra”. Métodos: Se utilizó 42 ratones, según expertos, de 3 meses de edad y 31 ± 4,4 g de peso, distribuidos aleatoriamente en seis grupos (n=7). Todos los grupos recibieron la misma dieta balanceada y agua ad libitum durante 5 días. Recibieron los siguientes tratamientos, por cinco días, vía peroral: grupo I y II: suero fisiológico (NaCl 0,9g% 10mL/kg), grupo III: vitamina E 400mg/kg, grupo IV: almendra 100 mg/kg, grupo V: almendra 500 mg/kg y grupo VI: almendra 1000 mg/kg; 12 horas antes de finalizar el Tto. se cortaron los bigotes de los ratones, excepto al grupo I; y luego de 12 horas se realizó el sacrificio. Principales medidas de los resultados: Nivel de lipoperoxidación expresado en sustancias reactivas al ácido tiobarbitúrico (TBARs) y nivel de Grupos sulfhídrilos no proteicos (GS-NP), además de cambios histopatológicos de tejido de cerebro y cerebelo. Resultados: La administración de Prunus dulcis “almendra” aumenta significativamente (p<0.05) los niveles de GS-NP en todos los grupos (excepto G VI) en comparación con el G II en cerebro; los niveles de TBARs disminuyen significativamente (p<0.05) en el grupo V y VI comparado con el grupo II, y en relación a los cambios histológicos se observa una mejora leve en el G V en comparación con el GII. Conclusiones: La administración de la suspensión de la semilla del Prunus dulcis “almendra” expreso un efecto neuroprotector en los indicadores bioquímicos (TBARs y GS-NP), sobre el tejido nervioso en ratones inducidos a estrés por desorientación motora. / Objetive: Determine the neuroprotective effect of administration of Prunus dulcis seed "almond" on the nervous tissue in motor stress induced disorientation mice. Design: Analytical, transverse, experimental and prospective study. Location: Laboratories of the Research Center of Biochemistry and Nutrition Alberto Guzman Barron, Faculty of Medicine, UNMSM, Lima, Peru. Materials: Mice albino BALB / c (Mus musculus) males and Prunus dulcis "almond". Methods: 42 mice was used, experts say, 3 months and 31 ± 4,4 g in weight, randomized into six groups (n = 7). All groups received the same balanced diet and water ad libitum for 5 days. They received the following treatments for five days, perorally: group I and II: saline (NaCl 0.9g% 10 mL / kg), group III: Vitamin E 400 mg / kg, group IV: almond 100 mg / kg, group V : almond 500 mg / kg and group VI: almond 1000 mg / kg; 12 hours before the end of Tto. Cut whiskers of mice, except the group I; and after 12 hours they were sacrificed. Main outcome measures: Level of lipid peroxidation expressed in thiobarbituric acid (TBARS) and level of non-protein sulfhydryl groups (GS-NP) substances in addition to histopathological changes of brain tissue and cerebellum. Results: Administration of Prunus dulcis "almond" significantly increased (p <0.05) levels of GS-NP in all groups (except G VI) compared to the G II in brain; TBARS levels decreased significantly (p <0.05) in the V and VI group compared with group II, and in relation to the histological changes seen a slight improvement in the GV compared to GII. Conclusions: The administration of the suspension of Prunus dulcis seed "almond" demonstrated the neuroprotective effect in biochemical (TBARs y GS-NP) on the nervous tissue in mice induced to stress motor disorientation. KEYWORDS: Prunus dulcis "almond" non-protein sulfhydryl groups (GS-NP), lipid peroxidation (TBARS), histopathological changes, disorientation motor stress.

牛樟芝 (Antrodia camphorata; AC) 為台灣特有的藥用真菌,是常見的傳統 中藥,富含三萜類化合物及多醣體。本研究使用牛樟芝、葛花、枳椇子與山楂共 四種萃取物製成之牛樟芝複方飲品 (A. camphorate drink, ACD)為原料,評估此一 複方飲品之抗疲勞功效。本研究從運動表現、疲勞生化指標、肌肉損傷生化指數 以及能量儲存等做為抗疲勞評估標準。將 5 週齡雄性 ICR (Institute of Cancer Research) 小鼠,分為三組,每組各 10 隻,分組如下:(1) 對照組 (vehicle, V); (2) 餵食 15.625 mL/kg 牛樟芝複方飲品 (ACD-1X);(3) 餵食 31.25 mL/kg 牛樟芝 複方飲品 (ACD-2X),連續經口餵食 28 天後進行以下試驗: 1. 測試小鼠前肢抓 力、2. 測試 5%負重游泳力竭時間、3. 進行游泳運動 15 分鐘後立即進行採血觀 察乳酸、血氨、血糖及肌酸激酶、4. 小鼠犧牲後,採集肝臟及肌肉組織進行肝醣 分析與切片。結果顯示,ACD-1X 組 (1124 g) 與 ACD-2X 組 (125 6 g) 在前肢 抓力與對照組 (95 6 g) 相比,分別顯著提升 1.19 與 1.32 倍 (p < 0.05)。ACD-1X 組 (56.714.3 min) 與 ACD-2X 組 (60.717.7 min) 在游泳力竭時間與對照組 (18.23.7 min) 比較分別延長 3.12 及 3.34 倍,均有顯著差異 (p < 0.05)。游泳運 動結束後,ACD-1X 組與 ACD-2X 組之乳酸、血氨及肌酸肌酶均顯著低於對照組 (p < 0.05),而血糖方面則顯著高於對照組(p < 0.05)。肌肉肝醣部分,ACD-1X 組 (1.79 ± 0.12 mg/g) 與 ACD-2X 組 (2.36 ± 0.21 mg/g) 相較於對照組 (1.54 ± 0.06 mg/g),2倍牛樟芝複方飲品組顯著提升1.53倍。肝臟肝醣部分,ACD-1X組 (48.9± 4.1 mg/g) 與 ACD-2X 組 (54.1 ± 5.8 mg/g) 與對照組 (27.0 ± 2.4 mg/g) 比較分別 增加 1.18 及 2.00 倍。研究結果顯示,牛樟芝複方飲品具有增加肝臟及肌肉組織 中的肝醣儲存量,且在游泳運動過程中降低血乳酸、血氨及肌酸激酶的產生。進 一步延長負重游泳力竭的時間及前肢抓力表現。因此,本研究證實牛樟芝複方飲 品具有抗疲勞及提升運動表現之功效。 / Antrodia camphorata is an endemic medical mushroom in Taiwan and has been reported to have multi-biological activities such as anti-fatigue, liver protection, anti-cancer, and anti-inflammation. This study was designed to ascertain the ergogenic and anti-fatigue properties of an A. camphorata drink (ACD) (comprising A. camphorata, Pueraria flos flowers, Hovenia dulcis Thunb, and Crataegus pinnatifida) by forelimb grip strength, load-weighted swimming test, and biochemical examinations in mouse model. Thirty male ICR mice (5-week-old) were randomly divided into three groups (n=10 per group): (1) vehicle; (2) a recommended dosage of 15 mg per 75 mL once per day for adult human (ACD-1X); (3) a 2-fold of recommended usage for adult human (ACD-2X). Mice were given orally either vehicle or A. camphorate drink by gavage for 28 days. The groups ACD-1X and ACD-2X showed significant increases in forelimb grip strength, swimming time to exhaustion skeletal, and muscle glycogen as compared to the vehicle group. Blood lactate and ammonia levels in the ACD-1X and ACD-2X groups were significantly lower than in the vehicle group post a swimming test. Moreover, the activity of plasma creatine kinase (CK), a marker of muscular damage, was significantly decreased in the ACD-1X and ACD-2X groups than vehicle group after swimming exercise. These results suggest that A. camphoratahas great potential for application in relevant fields for its ergogenic and anti-fatigue activities. / 第一章 緒論 .........................................................................................................................1 第一節 研究目的..............................................................................................................2 第二章 文獻探討 .................................................................................................................3 第一節 牛樟芝成份及相關研究......................................................................................3 第二節 葛花成份及相關研究..........................................................................................6 第三節 枳椇子成份及相關研究......................................................................................7 第四節 山楂成份及相關研究..........................................................................................8 第五節 運動與疲勞..........................................................................................................9 第三章 材料與方法 ...........................................................................................................12 第一節 實驗測試樣品....................................................................................................12 第二節 實驗動物之飼養與實驗流程............................................................................14 第三節 血液與組織樣本之收集與前處理....................................................................16 第四節 統計分析............................................................................................................17 第四章 結果與討論 ...........................................................................................................18 第一節 補充牛樟芝複方飲品對於小鼠游泳運動能力表現之測試............................18 第二節 補充牛樟芝複方飲品對於小鼠游泳運動後血液中乳酸、血氨、血糖濃 度以及 CK 活性之分析..................................................................................................19 第三節 補充牛樟芝複方飲品對於小鼠前肢抓力表現之測試....................................20 第四節 補充牛樟芝複方飲品對於小鼠肌肉與肝臟部位肝醣含量之分析................21 第五章 結論 .......................................................................................................................22 參考文獻.............................................................................................................31

The main objective of this dissertation is to build site index curves relating dominant height and age and to elaborate density management diagram (DMD) for individuals of Hovenia dulcis located in the central area of the state of Rio Grande do Sul, Brazil. To do that, it was used information from 4 stands, aged 25, located at Fundação de Pesquisa Agropecuária (FEPAGRO) and from a population of free trees, with DAPs varying from 5 to 45 cm, located at the campus of Universidade Federal de Santa Maria (UFSM), both in the municipal district of Santa Maria. For the construction of the sites indexes, information from 18 dominant trees was gathered in the 4 stands located at FEPAGRO, observing the methodology for trunk analysis. In places where trees were under competition, data for the elaboration of the density management diagrams were collected in trees of dominant, intermediate and dominated strata, considering the method of the punctual density. In the place where trees were free from competition, census was made. As a result, in the elaboration of the site index curves, the Backman model was selected, being verified the necessity of construction of two groups of index curves, from 5 to 25 years-old, with interval of 2 meters and index of 25. For the Site I-A, 3 index curves were created from 22 to 24 meters and, for the join of the Sites I-B+II+III, 6 index curves from 8 to 18 meters were produced. It was also possible to conclude that the Reineke model adjusted itself to calculate the closing density lines of the dossal and of the self-thinning, with angular coefficients very close to -1.5, for both of them, being demonstrated that the potency law of -3/2 of self-thinning was taken into account and that it was very similar to the hypothesis postulated by Reineke. The population density, for the beginning of the total capacity of the dossal, was of 42.5% of the maximum number of trees that the place allows, and that, with the collected data, it was possible to build diagrams for the density management, from the 5 to the 31 cm of diameter of the tree of medium basal area. / O objetivo principal deste trabalho é construir curvas de índice de sítio relacionando altura dominante e idade e elaborar diagramas de manejo de densidade (DMD), para populações de Hovenia dulcis localizadas na região central do estado do Rio Grande do Sul, Brasil. Para tanto, foram utilizadas informações de 4 povoamentos, com idades de 25 anos, localizado na Fundação de Pesquisa Agropecuária (FEPAGRO), e de uma população de árvores livres, com DAPs variando de 5 a 45 cm, localizado no campus da Universidade Federal de Santa Maria (UFSM), ambos no município de Santa Maria. Para a construção dos índices de sítios, coletaram-se informações de 18 árvores dominantes, nos 4 povoamentos localizados na FEPAGRO, observando a metodologia para análise de tronco. Nos locais onde as árvores estavam sob concorrência, os dados para a elaboração dos diagramas de manejo da densidade, foram coletados em árvores dos estratos dominante, intermediário e dominado, pelo método da densidade pontual. No local onde as árvores estavam livres de concorrência, realizou-se censo. Como resultado, na elaboração das curvas índice de sítio, o modelo de Backman foi o selecionado, ficando constatada a necessidade da construção de dois conjuntos de curvas índices, da idade de 5 a 25 anos, com intervalo de 2 metros e idade índice de 25 anos. Para o sítio I-A, foram geradas 3 curvas índice, de 22 a 24 metros e, para a união dos Sítios I-B+II+III, 6 curvas índices de 8 a 18 metros. Também foi possível concluir que o modelo de Reineke ajustou-se satisfatoriamente para estimar as linhas de densidade de fechamento do dossel e de autodesbaste, com coeficientes angular se aproximando muito de -1,5, para ambas, ficando demonstrado que a lei da potência de -3/2 de autodesbaste foi contemplada e que se aproximou muito da hipótese postulada por Reineke. A densidade populacional, para o início da total lotação do dossel, foi de 42,5% do número máximo de árvores que o local permite, sendo que com os dados coletados foi possível construir diagramas para o manejo da densidade, dos 5 aos 31 cm de diâmetro da árvore de área basal média.

Submitted by Johnny Rodrigues (johnnyrodrigues@ufcg.edu.br) on 2018-06-01T20:24:51Z No. of bitstreams: 1 SILMARA TAVARES BANDEIRA - DISSERTAÇÃO PPGSA PROFISSIONAL 2017..pdf: 1612468 bytes, checksum: 9d5ad6c257c433d58171497c7c9ba43c (MD5) / Made available in DSpace on 2018-06-01T20:24:51Z (GMT). No. of bitstreams: 1 SILMARA TAVARES BANDEIRA - DISSERTAÇÃO PPGSA PROFISSIONAL 2017..pdf: 1612468 bytes, checksum: 9d5ad6c257c433d58171497c7c9ba43c (MD5) Previous issue date: 2017-06-01 / Os óleos essenciais são misturas naturais, complexas de substâncias voláteis que possuem aroma intenso, são líquidos de aspecto oleoso a temperatura ambiente, mas se volatilizam em exposição ao ar em temperaturas específicas. Considerados como os agentes antimicrobianos mais importantes presentes nas plantas, os óleos essenciais possuem também propriedades antioxidante, anti-inflamatória, inseticida, entre outras. O óleo essencial de laranja constitui-se de compostos aromatizantes quimicamente instáveis. Estes se degradam facilmente na presença de luz, ar e umidade. Pensando nisso, este trabalho estudou a microencapsulação do óleo essencial de laranja doce (Citrus sinensis v. dulcis) utilizando maltodextrina e gelatina como encapsulantes de baixo custo, como alternativa para aumento da estabilidade, além de incentivar a agregação de valor às cascas, rejeito do qual o óleo essencial é obtido, com vistas à preservação ambiental. O estudo foi conduzido no Laboratório de Nutrição Animal, Centro de Saúde e Tecnologia Rural (CSTR), Universidade Federal de Campina Grande – UFCG, Campus de Patos – PB. Três formulações microencapsuladas foram desenvolvidas, em que os sistemas microencapsulados foram preparados com teor fixo de óleo essencial de laranja (10% m/m), variando-se a proporção de encapsulantes das formulações, em que a primeira incluiu apenas maltodextrina e as demais englobaram proporção de maltrodextrina e gelatina de 2:1 e 1:1, respectivamente. As formulações foram obtidas por emulsificação/liofilização. O estudo resultou em um pedido de patente (BR 10 2017 004722 9), depositado junto ao Instituto Nacional de Propriedade Industrial. O produto final obtido possui forte caráter de inovação tecnológica, além de maior segurança no manuseio, maior vida de prateleira e, portanto, o máximo de manutenção de integridade, somando-se, ainda, a possibilidade de inserção de um produto de alto valor agregado no mercado nacional, em que, dentre inúmeros setores, um dos grandes favorecidos é a indústria alimentícia, incluindo, ainda, o fortalecimento da interação entre Universidade e Indústria e também a preservação do meio ambiente. / Essential oils are natural, complex mixtures of volatile substances that have intense aroma; they are liquids of oily appearance at room temperature, but they volatilize in exposure to air at specific temperatures. Considered as the most important antimicrobial agents present in plants, essential oils also have antioxidant, anti-inflammatory, insecticidal properties, among others. Orange essential oil consists of chemically unstable flavoring compounds, which easily degrade in the presence of light, air and moisture. With this in mind, this work studied the microencapsulation of sweet orange essential oil (Citrus sinensis v. dulcis) using maltodextrin and gelatin as low cost encapsulants, as an alternative to increase stability, as well as to encourage the aggregation of value to the peels, from which the essential oil is obtained, aiming at environmental preservation. The study was conducted at the Animal Nutrition Laboratory, Center for Health and Rural Technology (CSTR), Federal University of Campina Grande - UFCG, Campus Patos - PB. Three microencapsulated formulations were developed in which the microencapsulated systems were prepared with fixed content of orange essential oil (10% m/m), varying the ratio of encapsulants of the formulations, in which the former included only maltodextrin and the others included proportion of maltodextrin and gelatin of 2: 1 and 1: 1, respectively. The formulations were obtained by emulsification / lyophilization. The study resulted in a patent application (BR 10 2017 004722 9), deposited with the National Institute of Industrial Property. The final product obtained has strong potential for technological innovation, as well as greater handling safety, longer shelf life and, therefore, the maximum integrity maintenance, also adding the possibility of insertion of a high - value product in the national market, where, among many sectors, one of the big beneficiaries is the food industry, including the strengthening of the interaction between University and Industry and also the preservation of the environment.