Made available in DSpace on 2016-08-29T15:37:21Z (GMT). No. of bitstreams: 1 tese_4888_.pdf: 792984 bytes, checksum: 55b49c255f7a7e0cb47a61520b8b1901 (MD5) Previous issue date: 2011-02-21 / O aumento da demanda em reprodução assistida de cães, bem como a crescente preocupação com a preservação de espécies ameaçadas de extinção têm impulsionado as pesquisas com reprodução canina, e o uso do cão como modelo experimental, em muitos casos, é um modelo mais relevante que os tradicionalmente utilizados para humanos. Além disso, a criopreservação de embriões caninos ainda é um desafio para a comunidade científica. Objetivou-se com este estudo avaliar as taxas de reexpansão da blastocele, taxas de eclosão, a viabilidade embrionária pós-descongelação e o desenvolvimento in vitro de blastocistos caninos criopreservados em glicerol 10% (GLI) e em etilenoglicol 1,5M (EG), por congelação lenta. Foram recuperadas 72 estruturas embrionárias e um total de 125 corpos lúteos foram identificados nos ovários, perfazendo uma taxa de recuperação embrionária de 57,6%. As concentrações plasmáticas de progesterona foram de 4,57±3,77 ng/mL no dia 0 (primeira cópula ou inseminação artificial) e 28,56±3,21 ng/mL no dia 12 (dia da colheita dos embriões). Cinquenta e um blastocistos foram separados aleatoriamente em dois grupos, GLI (n=26) e EG (n=25). Cada grupo foi subdividido em três (M0 = avaliação imediatamente após a descongelação, GLI = 9 e EG = 9; M3 = avaliação três dias após a descongelação e cultivo in vitro, GLI = 8 e EG = 8; e M6 = avaliação seis dias após a descongelação e cultivo in vitro, GLI = 9 e EG = 8). A criopreservação dos embriões foi realizada em máquina de congelação programável, em curva de resfriamento decrescente de 0,6°C até a temperatura de -35°C. Imediatamente após a descongelação, os embriões do M0 foram corados com a associação das sondas fluorescentes iodeto de propídeo (125 μg/ml) e Hoechst 33342 (1mg/ml) para avaliação da viabilidade celular; os embriões do M3 e M6 foram descongelados, cultivados in vitro em estufa com umidade saturada e atmosfera de 5% de CO2 em ar a uma temperatura de 38,5ºC, em meio SOFaa acrescido de 10% de soro fetal bovino (SFB), por três e seis dias, respectivamente, e corados de forma similar. A taxa de reexpansão da blastocele após 24h de cultivo in vitro não diferiu (P = 0,6196) entre os grupos GLI (76,5%) e EG (68,8%), respectivamente. Não foi observada eclosão embrionária em ambos os grupos. As taxas de viabilidade embrionária pós-descongelação dos grupos GLI e EG foram de 60,6±9,7 e 64,4±9,9, respectivamente, e não diferiram (P = 0, 8275). Além disso, não foi verificada diferença (P = 0, 3105) na viabilidade embrionária entre os momentos M0 (61,1±11,6%), M3 (50,0±12,4%) e M6 (76,4±12,0%). Conclui-se que a taxa de reexpansão da blastocele pode ser um indicativo de viabilidade embrionária pós-descongelação; que não há eclosão de blastocistos caninos criopreservados em glicerol 10% ou etilenoglicol 1,5M por congelação lenta e cultivados in vitro em meio SOFaa por até 6 dias; que blastocistos caninos criopreservados em glicerol 10% ou etilenoglicol 1,5M, por congelação lenta, apresentam viabilidade similar pós-descongelação e mantêm-se viáveis por até seis dias em cultivo in vitro.

Stem cell research offers hope to many people suffering from incurable diseases such as Alzheimer's disease, diabetes, heart disease and spinal back injuries. However this poses a moral dilemma because embryos are destroyed during embryonic stem cell research. To determine whether embryonic stem cell research is morally justifiable, two views in respect of a human being were considered: i. a human has a dualistic nature in which his body and soul are two separate entities or ii. his body and soul forms a unity which can not be separated. If a human has a dualistic nature, it means that the embryo is not a human, it does not have a soul because the soul is added later to form a human. The implication of this is that it will be morally justifiable to kill an embryo during embryonic stem cell research. However if body and soul forms a unity which can not be separated, the embryo is a human which is already developing into a full grown human with several stages of development. It will thus not be morally justifiable to kill an embryo as this will violate the sixth commandment, i.e. “Thou shalt not kill.” To determine whether a human’s body and soul is an inseparable unity or whether they are two separate entities, the Pauline letters' view on the human being was investigated. The research method employed was to do a comparative literary study to highlight the different aspects of stem cell research and then exegesis was done in respect of body (σoμα / sōma); soul (ψυχὴ / psychē) and spirit (πνεῦμα / pneuma) in the Pauline letters according to the grammatical-historical method. An electronic Bible Concordance was used to determine the texts in which the above concepts appear. A semantic word analysis was also done to analyse these concepts. Then authoritative commentaries were used to check the findings. The analysis indicated that Paul refers to a human as unity in which body and soul can not be separated. The implication of this finding is that embryonic stem cell research should be dismissed because it will result in the destruction of embryos. Humans will thus be killed in violation of the sixth commandment. On the other hand adult stem cell research should be encouraged because it has the potential to cure diseases which has up to now been incurable. / Thesis (MTh (Ethics))--North-West University, Potchefstroom Campus, 2013.

Stem cell research offers hope to many people suffering from incurable diseases such as Alzheimer's disease, diabetes, heart disease and spinal back injuries. However this poses a moral dilemma because embryos are destroyed during embryonic stem cell research. To determine whether embryonic stem cell research is morally justifiable, two views in respect of a human being were considered: i. a human has a dualistic nature in which his body and soul are two separate entities or ii. his body and soul forms a unity which can not be separated. If a human has a dualistic nature, it means that the embryo is not a human, it does not have a soul because the soul is added later to form a human. The implication of this is that it will be morally justifiable to kill an embryo during embryonic stem cell research. However if body and soul forms a unity which can not be separated, the embryo is a human which is already developing into a full grown human with several stages of development. It will thus not be morally justifiable to kill an embryo as this will violate the sixth commandment, i.e. “Thou shalt not kill.” To determine whether a human’s body and soul is an inseparable unity or whether they are two separate entities, the Pauline letters' view on the human being was investigated. The research method employed was to do a comparative literary study to highlight the different aspects of stem cell research and then exegesis was done in respect of body (σoμα / sōma); soul (ψυχὴ / psychē) and spirit (πνεῦμα / pneuma) in the Pauline letters according to the grammatical-historical method. An electronic Bible Concordance was used to determine the texts in which the above concepts appear. A semantic word analysis was also done to analyse these concepts. Then authoritative commentaries were used to check the findings. The analysis indicated that Paul refers to a human as unity in which body and soul can not be separated. The implication of this finding is that embryonic stem cell research should be dismissed because it will result in the destruction of embryos. Humans will thus be killed in violation of the sixth commandment. On the other hand adult stem cell research should be encouraged because it has the potential to cure diseases which has up to now been incurable. / Thesis (MTh (Ethics))--North-West University, Potchefstroom Campus, 2013.