The presented work entitled "Mobility of Human Populations at the End of the Stone Age" describes, generalizes, summarizes and subsequently evaluates the factors of migratory tendencies in prehistory - for the period of Neolithic over Eneolithic to Early Bronze Age. Emphasis is placed on the clarification of all interdisciplinary issues. Further on a detailed description of individual processes and archaeological approaches to them. As a basis for my own research, a database of data impacted by articles based on the study of strontium isotopes was created. In the next steps, the database was processed using statistical tools and its outputs were contingency tabletables and charts. Spatial assessment and their contexts were conducted in the GIS (Geographic Information System).