Multi-view video is capable of presenting a full and accurate depth perception of a scene. The concept of multi-view video is becoming more useful especially in 3D display systems by enhancing the viewing of high resolution stereoscopic images from arbitrary viewpoints without the use of any special glasses. Like monoscopic video, the multi-view video is faced with different challenges such as: reliable compression, storage and bandwidth due to the increased number of views as well as the high sensitivity to transmission errors. All these may lead to a detrimental effect on the reconstructed views. The work in this thesis investigates the problems and challenges of transmission losses in a multi-view video bitstream over error prone wireless networks. Based on the network simulation results, the proposed technique is capable of addressing the problem of transmission losses. In practical wireless networks, transmission errors are inevitable and pose a serious challenge to the coded video data. The aim of this research effort is to examine the effect of these errors in a multi-view video bitstream when transmitted over a lossy channel. Moreover, this research work aims to develop a novel scheme that can make the multi-view coded videos more robust to transmission errors by minimizing the error effects and improving the perceptual quality. Multi-layer data partitioning as an error resilient technique is developed in JMVC 8.5 reference software in order to make the multi-view video bitstream more robust during transmission. In addition to that, we propose a simple decoding scheme that can support the decoding of the multi-layer data partitioning bitstream over channels with high error rate. The proposed technique is benchmarked with the already existing H.264/AVC data partitioning technique. The work in this thesis also employs the use of group of pictures as a coding parameter to investigate and reduce the effects of transmission errors in multi-view video transmitted over a very high error rate channel. The experiments are carried out with different error loss rates in order to evaluate the performance of these techniques in terms of perceptual quality when transmitted over a simulated erroneous channel. Errors are introduced using the Sirannon network simulator. The error performance of each technique is evaluated and analysed both objectively and subjectively after reconstruction. The results of the research investigation and simulation are presented and analysed in chapter six of the thesis.

In this study, the novel idea of in-vivo directed evolution was applied in order to achieve variants of the enzyme galactose oxidase with increased activity. This procedure was done under starvation conditions in Escherichia coli BL21 Star (DE3). Previous studies have been carried out in order to improve the activity of this enzyme using directed evolution methods. In this study, the same idea was used in-vivo, during stationary phase adaptive mutations inside the host organism, hence called in-vivo directed evolution. This method gave variants with improved enzyme activity as compared with the wild-type enzyme, and some variants showed activities that were even higher than the variants of previous directed evolution studies, hence making this method a promising approach for the random mutagenesis of genes of interest. The above mentioned mutations are carried out by a special group of polymerases, the error-prone polymerases. Phylogenetic analysis of these error-prone polymerases was also carried out in order to investigate the relationship between the number of error-prone polymerases and the level of complexity of organisms, and both the number of error-prone polymerases and the ratio of error-prone polymerases to total DNA polymerases of six organisms were studied. It was found that as the organism gets more complex, the number of error-prone polymerases and their ratio to the total polymerases increase.

Stuttgart, Univ., Diss., 2008.

Stuttgart, Univ., Diss., 2005.

Universiẗat, Diss., 2000--Stuttgart.

No description available.

Background: HIV drug resistance is presumed to be inevitable due to the error-prone nature of the virus. However, poor adherence to the antiretroviral drugs is proven to be an impending factor for HIV drug resistance development. Of these two explanations, which is the most common reason for HIV drug resistance?Method: A total of 40 published studies about HIV drug resistance, were retrospectively collected in Pubmed (May 2017), from 36 different countries for this paper. From each study was participants, percentage of HIV drug resistance and HIV-1 subtype extracted for analysis. All studies were than classified by either high-income, middle-income or low-income, based on a country income status, defined by the World Bank. HIV drug resistance was tested against: continents, HIV-1 subtypes, number of study participants, income levels, GDP per capita and EWI’s. All statistical analysis was performed in R: The R project for statistical computing.Result: This paper show, that HIV drug resistance primarily is caused by poor adherence which is closely associated with drug stock out. Highest HIV drug resistance levels was found in middle-income countries. However, number of participants enrolled per study was important for the outcome and this indicates that HIV drug resistance would be higher in low-income countries if larger studied had been carried out in these settings. This means that there is a large unrecorded prevalence of HIV drug resistance in low-income countries.

abstract: With tremendous increase in the popularity of networked multimedia applications, video data is expected to account for a large portion of the traffic on the Internet and more importantly next-generation wireless systems. To be able to satisfy a broad range of customers requirements, two major problems need to be solved. The first problem is the need for a scalable representation of the input video. The recently developed scalable extension of the state-of-the art H.264/MPEG-4 AVC video coding standard, also known as H.264/SVC (Scalable Video Coding) provides a solution to this problem. The second problem is that wireless transmission medium typically introduce errors in the bit stream due to noise, congestion and fading on the channel. Protection against these channel impairments can be realized by the use of forward error correcting (FEC) codes. In this research study, the performance of scalable video coding in the presence of bit errors is studied. The encoded video is channel coded using Reed Solomon codes to provide acceptable performance in the presence of channel impairments. In the scalable bit stream, some parts of the bit stream are more important than other parts. Parity bytes are assigned to the video packets based on their importance in unequal error protection scheme. In equal error protection scheme, parity bytes are assigned based on the length of the message. A quantitative comparison of the two schemes, along with the case where no channel coding is employed is performed. H.264 SVC single layer video streams for long video sequences of different genres is considered in this study which serves as a means of effective video characterization. JSVM reference software, in its current version, does not support decoding of erroneous bit streams. A framework to obtain H.264 SVC compatible bit stream is modeled in this study. It is concluded that assigning of parity bytes based on the distribution of data for different types of frames provides optimum performance. Application of error protection to the bit stream enhances the quality of the decoded video with minimal overhead added to the bit stream. / Dissertation/Thesis / M.S. Electrical Engineering 2011

Mycolic acids are long chain lipids from the cell walls of Mycobacterium tuberculosis. The Nkuku phage display library was previously used to obtain monoclonal antibody binders to mycolic acids. In total 11 binders were obtained of which one was selected (MAC10) for further investigation by genetic engineering as presented in this dissertation. The antibodies of the Nkuku phage display library are in the format of single chain variable fragments (scFv). ScFv’s constitute only the epitope binding domains of an antibody consisting of the VH and VL domains fused into a single chain by a flexible linker protein. The selected anti-mycolic acid scFv is referred to as mycolic acid clone 10 (MAC10). Genes encoding the scFv’s of the Nkuku phage display library were cloned into the plasmid pHEN-1, a phage display vector. This vector is not commercially available or ideally suited for expression of scFv proteins. Therefore two vectors were investigated as possible targets for subcloning. The plasmids pGE20 and pAK400 were previously used for the expression of scFv antibody proteins. Subcloning into plasmid pAK400 proved to be the more efficient of the two investigated for subcloning. This subcloning yielded the recombinant plasmid pAKJS. Following the subcloning scFv protein expression was attempted using the plasmids pMAC10 (derived from pHEN-1) and pAKJS (derived from pAK400). Expression of MAC10 using plasmid pMAC10 in both Escherichia coli TG-1 and HB2151 was constitutive. This demonstrates that plasmid pHEN-1 is a non ideal vector as expression should not occur unless induced. Expression of MAC10 did not occur when pAKJS and Escherichia coli HB2151 were used. This was due to both the vector and expression host producing inhibitor protein for the Lac Z promoter controlling expression of the scFv. The MAC10 gene was subsequently randomized using the directed evolution method, error prone PCR. Sequence analysis of the five selected mutants indicated an average mutation rate of 8.6 mutations per 1000 base pairs. From the combined total of all five mutants, transversions made up the majority of substitutions. The majority of transversion mutations occurred at A-T base pairs. Transition substation mutations that made up the minority of total mutations occurred mostly at G-C base pairs. / Dissertation (MSc)--University of Pretoria, 2012. / Biochemistry / unrestricted

A central component of modern computing is the idea that computation requires determinism. Contrary to this belief, the primary contribution of this work shows that useful computation can be accomplished in an error-prone fashion. Focusing on low-power computing and the increasing push toward energy conservation, the work seeks to sacrifice accuracy in exchange for energy savings. Probabilistic computing forms the basis for this error-prone computation by diverging from the requirement of determinism and allowing for randomness within computing. Implemented as probabilistic CMOS (PCMOS), the approach realizes enormous energy sav- ings in applications that require probability at an algorithmic level. Extending probabilistic computing to applications that are inherently deterministic, the biased voltage overscaling (BIVOS) technique presented here constrains the randomness introduced through PCMOS. Doing so, BIVOS is able to limit the magnitude of any resulting deviations and realizes energy savings with minimal impact to application quality. Implemented for a ripple-carry adder, array multiplier, and finite-impulse-response (FIR) filter; a BIVOS solution substantially reduces energy consumption and does so with im- proved error rates compared to an energy equivalent reduced-precision solution. When applied to H.264 video decoding, a BIVOS solution is able to achieve a 33.9% reduction in energy consumption while maintaining a peak-signal-to-noise ratio of 35.0dB (compared to 14.3dB for a comparable reduced-precision solution). While the work presented here focuses on a specific technology, the technique realized through BIVOS has far broader implications. It is the departure from the conventional mindset that useful computation requires determinism that represents the primary innovation of this work. With applicability to emerging and yet to be discovered technologies, BIVOS has the potential to contribute to computing in a variety of fashions.