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The Autoradiographic Localization of Estrogen Binding Sites in Human Mammary LesionsBuell, Richard H. January 1984 (has links)
No description available.
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Oestrogen receptor subtypes in ovarian cancerWei, Na, 魏娜 January 2008 (has links)
published_or_final_version / abstract / Obstetrics and Gynaecology / Master / Master of Philosophy
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The identification and characterization of three distinct estrogen receptor subtypes in a teleost fish, the Atlantic croaker (Micropogonias undulatus)Hawkins, Mary Beth 28 August 2008 (has links)
Not available / text
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Genomic approaches to understanding oestrogen receptor alpha biologyRoss-Innes, Caryn Sarah January 2011 (has links)
No description available.
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Dynamics of oestrogen receptor regulation in breast cancerMohammed, Hisham January 2014 (has links)
No description available.
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Physiological factors affecting ovine uterine estrogen and progesterone receptor concentrationsPrater, Patrice L. 14 November 1990 (has links)
Two experiments were conducted to determine whether in
ewes uterine concentrations of estrogen and progesterone
receptors are affected by the presence of a conceptus or by
the hormonal milieu associated with extremes in photoperiod
to which ewes are exposed.
In Exp.1, nine mature ewes were unilaterally
ovariectomized by removing an ovary bearing the corpus luteum
(CL). The ipsilateral uterine horn was ligated at the
external bifurcation and a portion of the anterior ipsilateral
uterine horn was removed and assayed for endometrial nuclear
and cytosolic concentrations of estrogen receptor (ER) and
progesterone receptor (PR) by exchange assays. After a
recovery estrous cycle, ewes were bred to a fertile ram. On
day 18 of gestation a 10 ml jugular blood sample was collected
for measurement of serum concentrations of estradiol -17β (E₂)
and progesterone by radioimmunoassay. Ewes were
relaparotomized on day 18 and the remaining uterine tissue was
removed. Endometrium from both the pregnant and nonpregnant
uterine horn was assayed for nuclear and cytosolic ER and PR
concentrations. Nuclear and cytosolic ER concentrations on
day 10 of the cycle were greater than in endometrium of gravid
and nongravid uterine horns on day 18 of gestation (p<.01).
Endometrial nuclear PR levels were also greater on day 10 of
the cycle than in the pregnant (p<.05) and nonpregnant horn
(p<.01) on day 18 of gestation. There were no differences in
nuclear and cytosolic ER and PR concentrations between the
pregnant and nonpregnant uterine horn on day 18. Serum levels
of E₂ and progesterone on day 18 of gestation were 16.56 ±
2.43 pg/ml and 1.74 ± 0.57 ng/ml, respectively. These data
suggest that duration of exposure of the uterus to
progesterone and(or) the presence of the conceptus causes a
reduction in uterine concentrations of ER and PR. Further,
an effect of the conceptus, if any, is exerted via a systemic
route.
In Exp. 2, ten mature ewes were bilaterally
ovariectomized in early October. During the onset of the
winter solstice (late December), a 10 ml blood sample was
collected from five ewes for analysis of serum levels of E₂
and progesterone. Ewes were then laparotomized and
approximately one-third to one-half of a uterine horn was
removed and assayed for endometrial nuclear and cytosolic ER.
The contralateral horn was ligated at the external bifurcation
and 10 μg of E₂ in 3 ml of physiological saline was injected
into the uterine lumen of the ligated horn. After 48 h, a
jugular blood sample was collected for steroid analysis and
a section of the E₂ treated horn was removed and assayed for
endometrial cytosolic and nuclear ER. This procedure was
repeated on the remaining five ewes during the height of the
summer solstice (late June). Endometrial nuclear and
cytosolic concentrations of ER prior to and after exogenous
E₂ stimulation were similar during the winter and summer
solstice (p>.05). However, treatment with E₂ increased
endometrial nuclear and cytosolic concentrations of ER
compared with those of the nonstimulated uterine horn during
the winter and summer solstice (p<.05 for each). Serum levels
of E₂ prior to luminal treatment of ewes with E₂ during the
winter and summer solstice did not differ (16.55 ± 4.05 vs
16.00 ± 3.0 pg/ml, respectively, p>.05). Serum levels of E₂
48 h after administration of E₂ did not differ among ewes at
the winter and summer solstice (18.75 ± 2.4 vs 18.65 ± 1.65
pg/ml, respectively, p>.05). Serum levels of progesterone
were basal (<0.10 ng/ml) and did not differ in ewes prior to
and after E₂ treatment at the winter and summer solstice
(p>.05). These data indicate that physiological factors
and(or) hormones such as prolactin and melatonin secreted in
response to extremes in photoperiod do not appear to influence
uterine concentrations of ER in ovariectomized ewes. / Graduation date: 1991
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Bone mass in Chinese women around the menopause: the role of estrogen receptor beta gene polymorphisms andenvironmental risk factorsGu, Jing, 谷靜 January 2006 (has links)
published_or_final_version / abstract / Medicine / Master / Master of Philosophy
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Distribution of estrogen and progesterone receptors in the primate ovary, with emphasis on subpopulations of cells within the corpus luteum.Hild-Petito, Sheri Ann. January 1988 (has links)
Both estradiol and progeterone are proposed autocrine or paracrine regulators of ovarian function in primate species. However, specific receptors for these steroids have not been localized to individual compartments of the primate ovary. Using immunocytochemical techniques, estradiol receptors were detected in the germinal epithelium, but not other structures, of ovaries obtained from rhesus or cynomolgus monkeys during the follicular and luteal phases of the menstrual cycle. In contrast, progesterone receptors were present in stromal and interstitial tissue, the thecal layers of healthy and atretic follicles, as well as the functional corpus luteum. These results are consistent with the concept of a receptor-mediated role for progesterone, but not estrogen, within the predominant gametogenic and endocrine structures, e.g., the follicle and corpus luteum, of the primate ovary. The recent discovery of distinct cell types in the corpus luteum of domestic ungulates has revised concepts on the control of luteal function in these species. Studies were designed to test the hypothesis that the primate corpus luteum consists of cell subpopulations that differ in physical characteristics, function and regulation. Cells enzymatically-dispersed from the monkey corpus luteum at mid-luteal phase of the menstrual cycle differed in size (diameter) and the presence of the steroidogenic enzyme, 3β-hydroxysteroid dehydrogenase (3β-HSD). Analysis of dispersed cells for forward and 90° light scatter properties by flow cytometry revealed two distinct continua (Cα and Cβ). These continua were isolated using the sorting capabilities of the flow cytometer. Cα contained single cells of ≤ 15 μm and cell clusters; the cells were typically 3β-HSD-negative nonsteroidogenic. Cβ consisted of single cells that increased in size up to 40 μm and were 3β-HSD-positive. Cβ was divided into two regions (R₁ and R₃) and the cells isolated. R₁ cells were ≤ 15 μm whereas R₃ cells were ≥ 20 μm. Basal progesterone and estrogen production by R₃ cells was greater than that produced by R₁ cells (as determined by radioimmunoassay of the incubation media). Relative stimulation of progesterone production by hCG, cAMP or PGE₂ was not different between R₁ and R₃ luteal cells. These results support the hypothesis that the primate corpus luteum consists of distinct cell subpopulations which differ in size and steroidogenic capacity. However, the cell types which secrete progesterone are typically responsive to gonadotropin and PGE₂, possibly via a cAMP-mediated pathway.
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ESTROGEN BINDING SITES IN HUMAN NEOPLASIA; DETECTION USING A MORPHOLOGIC TECHNIQUE.PENNY, ROBERT JAMES. January 1984 (has links)
Thirty six cases of human endometrium (9 normals, 9 adenomatous hyperplasias and 18 cancers) were established as finite monolayer tissue cultures. All were evaluated for the presence of estrogen binding sites (EBS) by an indirect immunofluorescent (IF) technique. Positive EBS were identified in 33% of normals, 67% of hyperplasias and 62% of cancers. Serial subpassage EBS evaluation was performed in fourteen cases. In was observed that the stability of EBS positivity in vitro was related to type of endometrium rather than culture longevity (2 of 2 normals, 2 of 4 hyperplasias and 1 of 8 cancers remained positive throughout the period of study). Twelve of the cancers were studied for estrogen receptor by cytosol assay and 11 (91.6%) correlated with the IF marker method. Thirty-eight cases of breast cancer were studied for EBS by a direct cytochemical and immunofluorescent technique. Evaluation by the direct method proved to be consistent and easy in performance. Morphologic positivity was 60% with the indirect method and 94.7% with the direct method. Correlation with the chemical cytosol was 77% with the indirect method and 86.8% with the direct method. These results confirm and compare favorably with other reported studies of morphologic methods. It was suggested that attention should be directed to cellular localization of receptors as a possible means for predicting endocrine responsiveness of neoplasms. Cancers from tissues presumed to be target-variants for estrogen stimulation were investigated with the direct cytochemical technique to determine if EBS were present. Forty-eight randomly selected tumors from multiple organ systems were assessed for EBS. Appropriate control tests were used to determine specificity. A total of 23 of the 48 cases were interpreted as positive for EBS. These sites were localized to cytoplasmic, nuclear and nucleolar cellular compartments. Estrogen and progesterone receptors in patients with breast carcinoma are of value in the selection of patients for hormonal adjuvant therapy. Whether this will prove to be true for EBS in a variety of neoplasms is currently unknown and is worthy of investigation.
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Neuroanatomical distribution of androgen and estrogen receptors in the brain of the roughskin newt, Taricha granulosaDavis, Glen Andrew 07 December 1994 (has links)
The gonadal steroids, testosterone and estradiol, are known to be important
modulators of neuronal functions and behaviors in most vertebrate species. These
steroid hormones also elicit changes in neuropeptide synthesis and secretion, alter
specific neurohormone receptor levels, and alter neuronal morphology and
electrophysiology. Many of the actions of androgens and estrogen are mediated by
specific intracellular receptors found in certain regions of the brain. But where are
these neuronal targets for androgens and estrogen found?
The research in this thesis investigates the neuroanatomical distribution of
androgen and estrogen receptors in the brain of a urodele amphibian, the roughskin
newt, Taricha granulosa. Using immunocytochemistry with antibodies against these
receptors, the distribution of both androgen and estrogen receptor-immunoreactive
cells is described in the brain of this species. This study found brain regions that
contain immunoreactive androgen receptors that have not previously been reported in
poikilothermic vertebrates using other techniques.
In addition, the distribution of estrogen receptor-immunoreactive cells in
most brain areas, and the distribution of androgen receptor-immunoreactive cells in
several brain areas, were found to be similar in this amphibian to those described in
studies that employed in vivo autoradiographic techniques in other vertebrate species.
This study suggests that the neuroanatomical distribution of gonadal steroid receptors
is a relatively conserved trait in vertebrates. The widespread distribution of these
receptors in the brain probably reflects the multiple functions that androgens and
estrogen are known to have in the brain. / Graduation date: 1995
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