Main topic of this thesis is development of software for capture and analysis of RFID communication in UHF band (standard EPC Class-1 Generation-2). One part of the work is focused on the basics of the RFID systems, software defined radio concept and graphical programming language LabVIEW. Main part of this thesis is discussing the developed software itself, its methods and principles. Next part is dedicated to present the graphical user interface of created application and its functionality. Last part of this thesis contains examples of captured and analysed processes in UHF RFID communication.