No / Bio-energy availability, its potential and production, challenges, and opportunities in Europe are addressed in this chapter. In addition, a comprehensive comparison of different generations of biofuels, types of bioenergy resource availability for various EU countries, and the prediction of the supply rate has been explained. This book chapter helps us understand the progress of consumable bioenergy resources and expected future trends, which is highly important to know. The basis of this chapter is on the agreements in the European energy institutes and related policies. Furthermore, the potential resources of bio-energy in Europe, environmental impacts, and the challenges and barriers that may be faced in the present and future have been investigated.