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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Ocorrência de Listeria monocytogenes (Murray, 1911) em queijos artesanais comercializados na cidade de Manaus – AM

Guedes, Victoria Teresa de Morais 31 March 2003 (has links)
Submitted by Geyciane Santos (geyciane_thamires@hotmail.com) on 2015-09-04T11:04:45Z No. of bitstreams: 1 Dissertação - Victoria Teresa de Morais Guedes.pdf: 1880027 bytes, checksum: daafec25fd99e7a016851bdf562f4058 (MD5) / Approved for entry into archive by Divisão de Documentação/BC Biblioteca Central (ddbc@ufam.edu.br) on 2015-10-02T19:39:21Z (GMT) No. of bitstreams: 1 Dissertação - Victoria Teresa de Morais Guedes.pdf: 1880027 bytes, checksum: daafec25fd99e7a016851bdf562f4058 (MD5) / Approved for entry into archive by Divisão de Documentação/BC Biblioteca Central (ddbc@ufam.edu.br) on 2015-10-02T19:46:53Z (GMT) No. of bitstreams: 1 Dissertação - Victoria Teresa de Morais Guedes.pdf: 1880027 bytes, checksum: daafec25fd99e7a016851bdf562f4058 (MD5) / Made available in DSpace on 2015-10-02T19:46:54Z (GMT). No. of bitstreams: 1 Dissertação - Victoria Teresa de Morais Guedes.pdf: 1880027 bytes, checksum: daafec25fd99e7a016851bdf562f4058 (MD5) Previous issue date: 2003-03-31 / CAPES-Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / The occurrence of Listeria monocytogenes in cheeses craft type Coalho, Manteiga and Mussarela marketed in the city of Manaus - AM was researched. The collection of the 39 samples of the craft cheeses was made in a random way in free markets and they happened in the period of May to August of 2002. The microbiological analyses were accomplished at the Laboratory of Microbiology of Faculdade de Farmácia. The applied methodology the this research was adapted of Bacteriological Analytical Manual (BAM, 2001) and of the Compendium of Methods goes the Microbilogical Examination of Foods (APHA, 1992), Consisting of two pré-enrichments, before the selective isolation. Of the 39 collected samples, 19 (49%) samples were of cheese type Coalho, 14(36%) of cheese type Manteiga and 6(15%) of cheese type Mussarela. The absence of Listeria monocytogenes was observed in 100% of the samples, in the ones which 13 (33,33%) they were negative in the secondary enrichment and 26 (66,66%) in the isolation in Ágar OXA and Ágar PALCAM. The absence could be explained by the presence of some factors that they propitiate the inhibition of this bacterium, such as: the index very loud of Enterococcus spp. and also the presence some microorganisms producing of bacteriocins and commonly found in the milk, as the Lactobacillus lactis. By the inhibition possibility for coliformes, they were made countings of NMP/g for total coliformes, fecal coliformes and Escherichia coli, in 10 samples of cheeses of the type Manteiga and the obtained results were 70% of the polluted samples for total coliformes, 100% for fecal coliformes and Escherichia coli. Indicating the possibility, among other factors of inhibition of the Listeria monocytogenes in the analyzed cheeses. Then, in agreement with the cases told in the inhibition literature by polluting microbiota and the inadequate hygienic-sanitary conditions during the production, manipulation, packing, stockpiling and commercialization of the craft cheeses can be ended that all those factors might have inhibited the cresceimento of Listeria monocytogenes. / A ocorrência de Listeria monocytogenes em queijos artesanais tipo Coalho, Manteiga e Mussarela comercializados na cidade de Manaus - AM foi pesquisada. A coleta das 39 amostras dos queijos artesanais foi feita de modo aleatório em feiras livres e ocorreram no período de maio a agosto de 2002. As análises microbiológicas foram realizadas no Laboratório de Microbiologia da Faculdade de Farmácia. A metodologia aplicada a esta pesquisa foi adaptada do Bacteriological Analytical Manual (BAM, 2001) e do Compendium of Methods for the Microbilogical Examination of Foods (APHA, 1992), Constando de dois pré enriquecimentos, antes do isolamento seletivo. Das 39 amostras coletadas, 19 (49%) amostras eram de queijo tipo Coalho, 14(36%) de queijo tipo Manteiga e 6(15%) de queijo tipo Mussarela. A ausência de Listeria monocytogenes foi observada em 100% das amostras, nas quais 13 (33,33%) foram negativas no enriquecimento secundário e 26 (66,66%) no isolamento em Ágar OXA e Ágar PALCAM. A ausência poderia ser explicada pela presença de alguns fatores que propiciam a inibição desta bactéria, tais como: o índice muito alto de Enterococcus spp. e também a presença alguns microorganismos produtores de bactericionas e comumente encontrado no leite, como o Lactobacillus lactis. Mediante a possibilidade de inibição por coliformes, foram feitas contagens de NMP/g para coliformes totais, coliformes fecais e Escherichia coli, em 10 amostras de queijos do tipo Manteiga e os resultados obtidos foram 70% das amostras contaminadas por coliformes totais, 100% para coliformes fecais e Escherichia coli. Indicando a possibilidade, dentre outros fatores de inibição da Listeria monocytogenes nos queijos analisados. Então, de acordo com os casos relatados na literatura de inibição por microbiota contaminante e as condições higiênico-sanitárias inadequadas durante a produção, manipulação, embalagem, estocagem e comercialização dos queijos artesanais pode-se concluir que todos esses fatores podem ter inibido o cresceimento de Listeria monocytogenes.

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