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Denitrification in Flexibacter canadensisWu, Qitu January 1995 (has links)
Nitrate reductase (Nar) of F. canadensis is membrane-bound. Glucose is the most effective reductant to support nitrate uptake, and methyl and benzyl viologens are good electron donors to Nar both in intact cells and in membrane fractions. Nitrate uptake depends upon nitrate reduction, and requires the presence of active Nar. Nitrate transport depends upon the transmembrane pH gradient. / Oxygen reversibly inhibits nitrate uptake, and the minimal air saturation for this inhibition is about 2-4%. Oxygen inhibits denitrification at the level of nitrate transport rather than its reduction. The reduction of both nitric oxide (NO) and nitrous oxide by F. canadensis is relatively tolerant to oxygen, and its nitrite reductase (Nir) is much more sensitive to oxygen than the other reductases. Neither copper- nor heme-type Nir DNA probes from Pseudomonas species hybridized with the total DNA of F. canadensis, indicating that F. canadensis Nir may possess unique properties. / F. canadensis keeps the NO concentration very low under normal conditions. However, ionophores (carbonyl cyanide m-chlorophenylhydrazone (CCCP), carbonyl cyanide p-trifluoromethoxylphenylhydrazone (FCCP), and nigericin), high concentrations of nitrite, and low pH stimulate net NO production during reduction of nitrite. NO consumption by F. canadensis inhibited by several inhibitors. They are azide, cyanide, CCCP, FCCP, nigericin, sulfide, hydroxylamine, carbon monoxide, diethyldithiocarbamate, and Triton X-100. NO is toxic to Nor (nitric oxide reductase) only at concentrations $>$67 nM. / Studies on chloramphenicol inhibition of denitrification enzyme activity indicate that chloramphenicol inhibits denitrification at the levels of nitrate reduction and NO consumption in F. canadensis.
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Attachment of the pathogen Flexibacter columnaris to fish cells /Zaldivar, Mercedes. January 1985 (has links)
Thesis (M.S.)--Oregon State University, 1985. / Typescript (photocopy). Includes bibliographical references (leaves 60-65). Also available on the World Wide Web.
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Denitrification in Flexibacter canadensisWu, Qitu January 1995 (has links)
No description available.
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Hematology and histopathology of Coho salmon (Oncorhynchus kisutch) infected with Flexibacter psychrophilusKanchanakhan, Somkiat 30 November 1990 (has links)
Graduation date: 1991
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Erythrocytic inclusion body syndrome : salmonid stock susceptibility, secondary diseases, and vitamin therapyShanks, Carol A. 11 September 1991 (has links)
Erythrocytic inclusion body syndrome (EIBS) was artificially
established in selected stocks of juvenile fall and spring chinook
salmon (Oncorhynchus tshawytscha), chum salmon (0. keta), coho
salmon (0. kisutch), Atlantic salmon (Salmo salar), and rainbow
trout (0. mykiss). Adult spring chinook salmon were also
artificially infected with the EIBS virus. Adult male chinook had
higher prevalences of EIBS inclusion bodies than females.
Cytoplasmic inclusion bodies that are associated with EIBS were
not observed in steelhead (0. mykiss), brown (Salmo trutta) nor
brook (Salvelinus fontinalis) trout suggesting that these stocks are
less susceptible to the EIBS virus.
Coho salmon with EIBS were more susceptible to Flexibacter
psychrophilus, the causative agent of cold water disease (CWD)
than fish without EIBS. The fish with EIBS were most susceptible
to F. psychrophilus during the first 20 days after virus exposure,
when inclusion bodies were most prevalent. Coho salmon infected
with both the EIBS virus and F. psychrophilus required a longer
recovery period than fish exposed to either pathogen alone.
Most investigations of EIBS require in vivo experimentation
and artificial infections using diseased fish tissues. Heterologous
tissue used to establish EIBS did not contribute to anemia nor
mortality. Death was not attributed to the EIBS virus alone but to
the combined effects of the virus and a secondary pathogen.
The severity of EIBS may be reduced with dietary Vitamin C
prophylaxis. Fish fed 1,000 mg ascorbic acid/ Kg of diet had the
fewest signs of EIBS; they had the highest hematocrit values and
the lowest incidence of cytoplasmic inclusion bodies. However,
vitamin C therapy alone was not sufficient to prevent the disease. / Graduation date: 1992
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Hematological Parameters of the Bluegill, Lepomis machrochirus (Rafinesque), Including Effects of Turbidity, Chloramines, and Flexibacter columnarisJones, Betty Juanelle 05 1900 (has links)
Normal ranges of values for hematological parameters of bluegill gathered seasonally from three lakes were determined. Sexual, seasonal, and inter-lake variations were found. Effects of 2-wk exposure to turbidity on blood parameters included an increase in rbc size and a decrease in small lymphocytes. Effects of 3-hr exposure were increases in rbc count, hemoglobin, and pH and decreases in PG2 and large lymphocytes. The effects of 0.44 and 0.88 ppm chloramines were an increase in blood pH, a decrease in MEV, and severe spastic reactions resulting in loss of equilibrium or death in 90% of the fish. Effects of Flexibacter columnaris included an increase in transformed lymphocytes and a decrease in small lymphocytes.
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