No description available.

No description available.

Magister en Psicología Clínica de Adultos / Las investigaciones centradas en la persona del terapeuta se comienzan a desarrollar hace más de 20 años. Si bien las investigaciones en el campo de la Psicoterapia Experiencial han aumentado sustancialmente en los últimos 10 años, al considerar la relevancia de la variable del terapeuta en el desarrollo de los procesos de psicoterapia, se vuelve necesario fortalecer esta área de investigación. Así, la presente investigación se centró en cómo la práctica de focusing aporta al desarrollo de la persona del terapeuta humanista. Respecto a la variable del terapeuta, se consideraron las habilidades clínicas, el auto cuidado, la visión de cambio terapéutico y la visión de la alianza terapéutica como los principales aspectos a explorar. Para el desarrollo de la investigación se utilizó un diseño de investigación cualitativo de carácter exploratorio-descriptivo. La muestra consistió en 12 terapeutas humanistas que practicaran el focusing en el trabajo psicoterapéutico. La información obtenida fue analizada a través de un análisis de discurso, utilizando la codificación abierta y la codificación axial de la teoría fundamentada. El fenómeno central que emergió en el transcurso de la investigación consiste en un modo de aproximarse y de estar con la experiencia de un otro, el cual emerge y se desarrolla a partir de la integración de la práctica de focusing en el contexto psicoterapéutico. Se observó que este modo permea tanto la dimensión profesional como la personal, constituyéndose así como una forma de funcionar de la persona en su totalidad

Generating droplets using flow-focusing microfluidics in multiphase flows has reached its limit that it cannot generate submicrometer droplets in size. Flow focusing geometry together with an electric field has been used to make smaller droplets in microchannels. The droplet size was controllable by the flow rate ratio as well as the electric field. The droplets size decreased as the voltage increased. A Taylor cone was formed to generate very fine droplets which were less than 1mμ in diameter. The tip made smaller droplets due to the tangential force by the electric field. A small inner flow rate and high electric field were required to form a stable Taylor cone in a DC electric field. The droplet size, however, was not stable at a small water flow rate because the flow rate was not as accuate as required. When I used a modified syringe pump with more accurate flow rate control, I was able to obtain a stable set of data. A small change in droplet size occurred at low voltage. The drop size changed dramatically, when the voltage was high enough. I also observed how an AC electric field affects the droplet size. The droplet size was not solely determined by the voltage. This is because of the imbalance of the supplied flow rate and the emitted flow rate. I also found that the droplet size is related to the tip position of the dispersed phase. The droplet size decreased as the tip stretched more. Typically, the microfluidic device generated monodispese droplets in narrow size distribution. It also generated a bigger droplet followed by a smaller one consecutively at low flow rate ratio of inner and outer fluid flow ()265.0/09.0≤≤oiQQ. To understand this instability of drop formation, a numerical calculation was conducted. The simulation results showed inside of the tip still pointed downstream after it generated a big droplet. Then, the tip generated another smaller droplet while the tip was stretched. Finally, the tip moved back and began a new cycle.

Generating droplets using flow-focusing microfluidics in multiphase flows has reached its limit that it cannot generate submicrometer droplets in size. Flow focusing geometry together with an electric field has been used to make smaller droplets in microchannels. The droplet size was controllable by the flow rate ratio as well as the electric field. The droplets size decreased as the voltage increased. A Taylor cone was formed to generate very fine droplets which were less than 1mμ in diameter. The tip made smaller droplets due to the tangential force by the electric field. A small inner flow rate and high electric field were required to form a stable Taylor cone in a DC electric field. The droplet size, however, was not stable at a small water flow rate because the flow rate was not as accuate as required. When I used a modified syringe pump with more accurate flow rate control, I was able to obtain a stable set of data. A small change in droplet size occurred at low voltage. The drop size changed dramatically, when the voltage was high enough. I also observed how an AC electric field affects the droplet size. The droplet size was not solely determined by the voltage. This is because of the imbalance of the supplied flow rate and the emitted flow rate. I also found that the droplet size is related to the tip position of the dispersed phase. The droplet size decreased as the tip stretched more. Typically, the microfluidic device generated monodispese droplets in narrow size distribution. It also generated a bigger droplet followed by a smaller one consecutively at low flow rate ratio of inner and outer fluid flow ()265.0/09.0≤≤oiQQ. To understand this instability of drop formation, a numerical calculation was conducted. The simulation results showed inside of the tip still pointed downstream after it generated a big droplet. Then, the tip generated another smaller droplet while the tip was stretched. Finally, the tip moved back and began a new cycle.

No description available.

Polyacrymalide gel isoelectric focusing (PAGIF) in thin-layer was used to resolve proteins of Mycoplasma spp., Acholeplasma spp. and of eight strains of Ureaplasma urealyticum (T-Strain). A mixture of urea, Triton X-100, and dithioerythritol was used to solubilize sonically disrupted cells.

国立情報学研究所で電子化したコンテンツを使用している。

This thesis presents an analysis of the signal-to-noise ratio in liquid core anti-resonant reflecting optical waveguides (ARROWs) and the application of hydrodynamic focusing to the waveguides. These concepts are presented as a method to improve the detection capabilities of the ARROW platform. The improvements are specifically targeted at achieving single molecule detection (SMD) with the devices. To analyze the SNR of the waveguides a test platform was designed and fabricated. This test platform was then used to examine relationship between the SNR and the location of the excitation region. It was determined that the excitation region should be moved closer to the solid-core. By moving the excitation region closer to the solid-core the distance the signal was required to travel in the hollow-core was reduced. This reduction led to a decrease in optical signal loss and resulted in a more than 2x increase in the SNR. Hydrodynamic focusing in the waveguides was developed as a method to increase the consistency of detection of the devices. In hydrodynamic focusing particles in the sample are forced towards the center of the waveguide with a buffer solution. With the particles focused to the center of the channel the percentage that passed through the excitation region can be increased improving the detection consistency of the device. ARROW chips designed for hydrodynamic focusing were simulated, fabricated, and preliminary testing was performed. Initial results have shown a more than 30% increase in particle focusing.

No description available.