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The diploma thesis deals with the rate of free chlorine dacay in the water supply systems. In the general part of the work are mentioned the forms of chlorine that can be encountered in its use for disinfection of drinking water. Another chapter is the modeling of chlorine decomposition in water during pipeline distribution. This chapter deals with the kinetic reactions of chlorine and their orders, and subsequently with factors influencing the rate of chlorine loss. Closer deals with pipe wall chlorine decay and the bulk water flow chlorine decay. The second part of the diploma thesis is focused on determining the constant kb, which is the rate coefficient of loss of chlorine in the pipeline caused by the flow of water. The work contains several researches of various world studies that deal with this issue. The following is a step-by-step guide to performing this test. The last part deals with the case studies at the waterworks in Kateřinice and Brno. On these tapes the chlorine concentration was measured over time and a constant kb was determined based on these results, expressing the rate of free chlorine decay in bulk water flow.